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Citations for Cell Signaling Technology :
1151 - 1200 of 10000+ citations for 8 CHLORO 2 CHLOROMETHYL 6 TRIFLUOROMETHYL IMIDAZO 1 2 A PYRIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (rabbit anti-p53 [7F5] (Cell Signaling 2527S, Lot 8), mouse anti-Mdm2 [2A10] (Abcam ab16895 ...
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bioRxiv - Biochemistry 2022Quote: ... or rabbit pAb anti-phospho SMAD1/5/8 (#9511, Cell Signaling) at 1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated overnight at 4°C with the primary antibodies Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370S, Cell Signaling) at 1:2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Other commercially available primary antibodies used were: anti-Phospho-p44/42 MAPK (phosphorylated ERK1/2, Thr202/Tyr204, Cell Signaling Technology #4377), anti-p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit antibodies for anti-myosin-light-chain-2 (Cat #3672) and anti-phospho-myosin-light-chain-2 (raised against phospho-Thr18/Ser19; Cat #3674) were purchased from Cell Signaling and used at 1:500 (Western blot) ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Cell Biology 2022Quote: ... then seeded in DMEM/F12 and 1% serum plus antibiotic-antimycotic solution 48 hrs post-transfection cells were blocked and treated with Ab against α5β1 (Novus, 2-52680) and αv (Cell Signaling 4711) at 10ug/ml for 30 minutes prior to cell surface stripping (0.2M acetic acid ...
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bioRxiv - Neuroscience 2021Quote: Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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bioRxiv - Plant Biology 2021Quote: ... the membrane was incubated overnight with the primary phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, #9101). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... 10-15 μg of proteins were separated by SDS-PAGE and immunoblotted using the following antibodies: p-ERK1/2 (9101, Cell Signaling), ERK1/2 (4695 ...
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bioRxiv - Cancer Biology 2022Quote: ... Pre-cleared lysates were incubated over night at 4°C with end-over-end rotation with 2 µg of anti-Notch1 (D1E11, Cell Signaling) or anti-Cbl-b (G1 ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed with TBST prior to incubation for 2 hrs at room temperature in the appropriate secondary antibody (anti-rabbit or anti-mouse; Cell Signaling) at 1:2000 dilutions ...
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bioRxiv - Genetics 2022Quote: ... Beads were then resuspended in 250 μl blocking solution and 2 μg antibody (RAD21, Abcam ab992; CTCF, Cell Signaling Technology 3418) was added ...
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bioRxiv - Microbiology 2019Quote: ... Where stated, cells were treated with 2 μM ruxolitinib (INCB018424, Stratech, S1378-SEL) or 50 nM bortezomib (Cell Signaling Technology #2204) or 1000 units of IFNβ (pbl Assay Science ...
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bioRxiv - Microbiology 2020Quote: ... 250 μL of lysate were incubated with 2 μL of mouse Myc-Tag antibody clone 9B11 (Cell Signaling Technology, Cat. 2276S) for 4 h with rotation at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-rabbit IgG (H+L)-F(ab’)2 Fragment-Alexa Fluor 555 Conjugate (cat. No. 4413, Cell Signaling Technology, Beverly, USA), CRLS1 pAb (cat ...
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bioRxiv - Pathology 2020Quote: ... for one hour at 25°C and incubated overnight with the rabbit anti-phospho-MEK1/2 (Ser217/221) antibody (Catalog #9154; Cell Signaling), the mouse anti-MEK1/2 antibody (Catalog #4694 ...
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bioRxiv - Physiology 2021Quote: ... were subjected to western blot analysis with antibodies against c-Jun NH(2)-terminal kinase (JNK) and phospho-JNK (Cell Signaling). Immunocomplexes were detected using the Licor Odyssey secondary detection system.
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bioRxiv - Cancer Biology 2021Quote: ... the degree of staining of the tissue specimens was observed under the Zeiss Apotome.2 microscope (20X objective, 0.8 NA) after DAB staining (Cell Signaling Technology) and hematoxylin (IHC World ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with rabbit antibodies against phospho (S473)-AKT (p-AKT) and phospho (Thr202/Tyr204) p44/42 ERK1/2 (p-ERK) (4060, 9101; Cell Signaling) for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated with the primary antibodies: polyclonal rabbit antibody against PTP1B (2 μg/mL; #5311; Cell Signaling Technology, Boston, MA, USA) and monoclonal mouse antibody against Cdk3 (2 μg/mL ...
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bioRxiv - Plant Biology 2022Quote: ... Phosphorylated MAPK proteins were detected by immunoblot analysis with antiphospho-p44/42 MAPK (Erk1/2)(Thr202/Tyr204) (D13.14.4E) rabbit mAb (Cell Signaling Technology, USA). The blotted membrane was stained with Coomassie Brilliant blue (CBB ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated overnight at 4°C on a rocker with primary antibodies (Supplemental Table 2) according to the manufacturer’s instructions and then developed with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 hour at room temperature on an orbital shaker ...
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bioRxiv - Microbiology 2024Quote: ... They were passed through a 0.45 µm filter and precleared with 2 μg/ml anti-rabbit isotype IgG antibody (Cell Signaling #2729) with protein A/G magnetic beads (Pierce ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mg of lysate representing one biological replicate was incubated for 2 hours at 4 °C with anti-Kv11.1 antibody (Cell Signaling #12889) covalently coupled to protein A/G magnetic beads (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Double resistant cell lines with dual integration of 4EBP1/2 shRNAs were validated by western blot (Cell Signaling Technology #9452, #2845) for efficient stable knockdown of the targeted proteins.
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bioRxiv - Plant Biology 2023Quote: ... Blots for detecting phosphorylated MAPK were blocked in 5% BSA and immunoblotted with a phosphor-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling). Horseradish peroxidase-conjugated anti-rabbit antibody produced in goat (Agrisera ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then permeabilized and blocked with 0.1% saponin/0.1% BSA in PBS before incubated with α-SARS-CoV-2 Nucleocapsid primary antibody (Cell Signaling Technology) at 1:1000 in permeabilization/blocking buffer overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Cells were then permeabilized and blocked with 0.1% saponin/0.1% BSA in PBS before incubated with α-SARS-CoV-2 Nucleocapsid primary antibody (Cell Signaling Technology) at 1:1000 in permeabilization/blocking buffer overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... every 8th section of the brain was processed for double-fluorescent immunostaining for Ku80 and one of the following: Microtubule-associated protein 2 (MAP2; 8707, Cell Signaling), doublecortin (ab18723 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2 h blocking with 15% milk powder (in TBST) and overnight incubation with rabbit anti-BIP (3183, Cell signaling, 1/1000 for retina ...
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bioRxiv - Microbiology 2024Quote: ... The following antibodies were used for viral protein analysis: SARS-CoV-2 anti-Spike and anti-N protein (cat #569996 and #33336, respectively, Cell Signaling) and anti-β-actin (Sigma – cat #a2228) ...
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bioRxiv - Cell Biology 2020Quote: ... Phospho-LRP5/6 (Ser1493/Ser1490; Cell signaling, Cat.no. #2568), AXIN1 (Cell signaling ...
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bioRxiv - Immunology 2021Quote: ... Gata-6 (D61E4; Cell Signaling Technology Cat# 26452, RRID:AB_2798924) or purified polyclonal rabbit anti-Relmα (PeproTech Cat# 500-P214-50ug ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-p-S6 (S235/6) (Cell Signaling # 62016S), rabbit anti-RHEB (Fisher #PIPA520129 ...
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bioRxiv - Plant Biology 2020Quote: ... membranes were incubated with the antibody against Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® (Cell Signaling Technology, USA). As a loading control membranes were incubated with anti-tubulin antibody (Abiocode ...
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour and probed overnight at 4°C with the primary antibodies of interest directed against: rabbit anti-BCL-2 (Cell Signaling, CST4223), rabbit anti-BCL-xL (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% milk and incubated in 2% BSA with primary antibodies to GAPDH (Cell Signaling Technology Cat# 13084, RRID:AB_2713924), Eg5 (Sigma Aldrich Cat# SAB4501650 ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then blotted overnight with anti-human phospho-Akt and anti-human phospho-Erk1/2 mAbs (Cell Signaling, MA, USA), followed by anti-rabbit or anti-mouse horseradish peroxidase(HRP)-conjugated secondary mAb (ThermoFisher scientific ...
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bioRxiv - Systems Biology 2019Quote: ... Membranes were probed with rabbit monoclonal antibodies directed against the diphosphorylated (Thr202/Tyr204) form of ERK1/2 (p42/44 MAPK, Cat. No. 4372, Cell Signaling Technology) and total ERK1/2 (Cat ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with appropriate secondary antibodies for 1h: Alexa Fluor 488 or 555 or 647 Goat anti-Mouse IgG (2 μg/mL, Cell Signaling Technology). Cells were then permeabilized as previously described (Souriant et al. ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-human myosin light chain 2 (D18E2) [with cross-reactivity to mouse] (catalog # 8505, RRID:AB_2728760; Cell Signaling Technology, Danvers, MA, USA), and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Vero E6 hTMPRSS2 cells infected with SARS-Cov-2 were lysed and analyzed by Western blot as done in with Rabbit Anti-SARS CoV-2 (COVID-19) Spike (Cat No. GTX135356) (Gene Tex) and Rabbit anti-Vinculin #13901 (E1E9V XP®) (Cell signaling) as loading control ...
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bioRxiv - Pathology 2021Quote: ... The separated proteins were transferred onto a nitrocellulose membrane for detection with polyclonal rabbit anti-Phospho-Myosin Light Chain 2 (Ser19) antibody (Cell Signaling Technologies) or monoclonal rabbit anti-Myosin Light Chain 2 (D18E2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were spun down and washed three times with cold Flow Wash Buffer (FWB) (PBS, 2% FBS, 2mM EDTA) and incubated with primary antibody (EGFR-D1D4J, Cell Signaling Technology) diluted in FWB for 60 minutes on ice ...
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bioRxiv - Plant Biology 2022Quote: ... Protein electrophoresis and Western-Blot were performed as described previously using the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology) (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... was done overnight at 4°C and was followed by incubation with anti-mouse IgG (H+L) F(ab’)2 conjugated to Alexa Fluor 647 (Cell Signaling Technology). Images were acquired with ZEISS LSM 980 laser-scanning confocal microscope and analysed using ZEN software (Carl Zeiss MicroImaging).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells (2 x106/sample) were collected by centrifugation at 300 g for 4 min and resuspended in cell lysis buffer (Cell Signaling #9803) supplemented with protease inhibitors (Sigma #5056489001) ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies to EGR1, EGR3, phospho-ERK1/2, GAPDH and actin, and an inhibitor of EphA2 (dasatinib, 100 nM) were from Cell Signaling Technologies. Secondary HRP-conjugated secondary antibodies used were goat anti-rabbit (Jackson ImmunoResearch ...