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Citations for Cell Signaling Technology :
1151 - 1200 of 1230 citations for 3 Acetyl N ethylpyridinium bromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL of aprotinin and leupeptin] and incubated (16 hrs., 4°C) with either 3 µg non-immune control rabbit IgG (Cell Signaling #272) or with 3 µg of ERRα antibody (Cell Signaling #13826 ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in antibody solution containing 2% normal donkey serum and 0.3% Triton X-100 in PBS with primary rabbit anti-activated caspase 3 antibody (Cell Signaling, Cat# 9661). Sections were washed five times for five minutes in PBS ...
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bioRxiv - Immunology 2022Quote: ... Membranes were washed 3 times with TBST and then incubated with peroxidase-conjugated secondary antibody (Cell Signaling Technology, Cat.No. 7074S, 1:3000). The antigen-antibody reaction was visualized by enhanced chemiluminescence assay (ECL ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized with 0.3% Triton X-100 in PBS (PBST) prior to blocking and staining with rabbit anti-acetylated alpha tubulin antibody (Cell Signaling #5335). Confocal 3D images of whole islets were used for cilia frequency and length quantitation ...
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bioRxiv - Cell Biology 2022Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Physiology 2022Quote: ... The following day sections were washed in wash buffer 3 times for 5 min and co-stained for anti-Rabbit IgG Fab2 Alexa Fluor 488 (Cell Signaling, 4412S) at 1:1000 dilution at room temperature for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed in TBS-T (3 × 5 min) and probed with secondary antibodies: mouse (Cat #: 7076S; Cell Signaling Danvers, MA) or rabbit (Cat # ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated overnight at 4°C with primary antibody detecting light chain 3 type 1 and 2 (Cell Signaling technology, catalog #3868), SOAT1 (catalog #84476) ...
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bioRxiv - Cell Biology 2024Quote: ... Crude cell extract was lysed with an insulin syringe and pre-cleared by incubation with 3 μg Normal Rabbit IgG (Cell Signaling Technology) and 10uL Pierce™ Protein A/G Magnetic Beads (Thermo Scientific 88803 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rabbit, pan, 40D4), phospho-AKT [rabbit, Ser473, (D9E)XP] and GAPDH (glyceraldehyde-3-phosphate dehydrogenasemouse, D4C6R) were purchased from Cell Signaling (USA). Antibodies against human PCNA (proliferating cell nuclear antigen ...
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bioRxiv - Cancer Biology 2024Quote: ... Smad2/3 (#8685S), HIF-1α (#14179S), HIF-2α (#7096S), ASCT2 (#8057) and E-cadherin (#3195S, #14472S) antibodies were obtained from Cell Signaling Technology for immunoblot or fluorescence imaging analysis ...
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bioRxiv - Bioengineering 2024Quote: ... The antibodies against phospho-STAT3 (Tyr705, 9145), cleaved caspase-3 (Asp175, 9579) and PCNA (2586) were purchased from Cell Signaling Technology (USA). The antibodies against myeloperoxidase (ab208670 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation for 1–3 hr at RT with 1:5000 secondary antibody (Cell Signaling Technology goat HRP-linked Anti rabbit IgG #7074), and 3 more 5 min washes with TBST ...
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bioRxiv - Developmental Biology 2023Quote: Immunofluorescence was performed according to the protocol described in the data sheets of the anti-acetylated histone 3 lysine 27 (H3K27Ac) antibody (# 8173, Cell Signaling Technology) and anti-acetylate histone 3 lysine 9 (H3K9Ac ...
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bioRxiv - Cancer Biology 2023Quote: ... cleaved caspase-3 (#9662S, 1:500), GRP78 (BiP, #3177, 1:1000), phospho-eIF2α Ser 51 (#3398, 1:1000) from Cell Signaling Technology Inc ...
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bioRxiv - Cell Biology 2023Quote: ... washed with TBST (3 ×10 min), incubated (1 hr, 20°C) in TBST containing HRP-conjugated secondary antibody (1:3000 anti-mouse, Cell Signaling Technology Cat# 7076S ...
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bioRxiv - Cancer Biology 2023Quote: ... probed with primary antibodies (Supplementary Table 3) and detected with horseradish peroxidase-conjugated α-rabbit or α-mouse secondary antibodies (Cell Signaling). The blots were imaged using a ChemiDoc MP Imaging system (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: Immunoblot experiments were performed as previously described (17, 18) using antibodies against cleaved caspase-3 (Cell Signaling Technology, Cat#9664, RRID: AB_2070042), procaspase-3 (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-SF3B1 (1:500 dilution; D221-3, MBL Life Science) and anti-phosph-SF3B1(Thr313) (1:500 dilution; D8D8V, Cell Signaling Technology). After washing three times with PBS ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then labeled overnight at 4℃ with primary antibodies for neurofilament-heavy (RT-97, Developmental Studies Hybridoma Bank, University of Iowa) or cleaved caspase-3 (Cell Signaling 9664S). After three rounds of washing ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated for 45 min with 3 μg/ml Myc-Tag (9B11) Mouse mAb AlexaFluor 488 conjugate antibody (Cell Signaling Technology) and Phalloidin-Alexa 647 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for more than 3 hours with a protein complex of anti-HA antibody (Cell signaling technology) and Dynabeads Protein A (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2024Quote: ... Cells were treated and prepared as described above for synchronization and flow cytometry with the addition of cleaved caspase-3 antibody (Cell Signaling Technology) conjugated to Alexa Fluor 647 added together with pH3 antibody ...
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bioRxiv - Immunology 2024Quote: ... Samples were blocked in 1 % BSA in PBS for 3 h in a hydration chamber followed by primary antibody staining with pStat1 (Tyr701, Cell Signaling #9167) at 1:200 dilution overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The required primary antibody (JOSD1 [Abcam, Cambridge, United Kingdom], SOCS1 [Abclonal, Woburn, Massachusetts, United States of America], Cleaved Caspase 3 [Cell Signaling Technology, Danvers ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitation was carried out overnight at 4 °C using 5 μl of rabbit polyclonal FosB antibody (2251S, lot 3, Cell Signaling Technology) or 2 μg of rabbit polyclonal IgG isotype (3900S ...
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bioRxiv - Cell Biology 2024Quote: ... and directly lysed with RIPA buffer (150 mM NaCl, containing proteinase inhibitor cocktail 1 and 3 and phosphatase inhibitor cocktail (Cell Signaling Technology) on ice for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The antibodies used in the study is as follows: anti-glutaminase-1/GLS1 (E9H6H) antibody and anti-cleaved caspase-3 (Asp175) antibody obtained from Cell Signaling Technology and rabbit secondary antibody conjugated to biotin obtained from Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Extracts were centrifuged at 800 × g at 4 °C for 5 min and then incubated with 1 μg of rabbit anti-PC4 antibody or with 3 μg of mouse monoclonal anti-Myc antibody (Cell Signaling Technology) for 3 h at 4 °C on a rotating wheel ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit DA1E monoclonal antibody IgG isotype control (cat. # 3900) and cleaved caspase-3 rabbit monoclonal antibody (cat.# 9664) were all from Cell Signaling Technology. Anti-Iba1/AIF1 mouse monoclonal antibody (used for immunofluorescence in xenografts ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were resuspended in 3% BSA in PBS containing a 1:1000 dilution of LAMP1 antibody (Rabbit-anti human LAMP1, Cell Signaling Technology,). The mixture was incubated for 1-2 h at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... The plates were then washed 3 times with PBST before adding 100 μl of TMB substrate solution (Cell Signaling Technology, USA, Catalog: 7004S) to the plates after aspirating out the PBST and was incubated for 25 minutes at room temperature on a plate shaker ...
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bioRxiv - Neuroscience 2020Quote: ... blocked in TBST with 3% BSA for 1 hour at room temperature and incubated in primary antibody solutions (in TBST with 3% BSA) directed against: phosphorylated (pY816, #4168, 1:1000; pY515, #9141, 1:1000; pY706-7, #4621S, 1:1000, Cell signaling technology (CST), Danvers ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500; mouse anti-NeuN, Millipore, 1:500; rabbit anti-cleaved caspase-3, Cell Signaling, 1:500) overnight in 3 % BSA in PBS at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... the retinal explants were immuno-stained against microtubule with beta-3 tubulin (TUBB3) antibody (1:250, from rabbit, Cell Signaling Technology, 5568S, USA), washed in 0.5% Triton in PBS for 5 times ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed with 1× TBST (3 × 10 min) and incubated with anti-mouse IgG horseradish-peroxidase secondary antibody conjugate (1:10000 dilution; Cell Signaling Technology, 7076S) in 1× TBST for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and then immunoprecipitated for 3 hours at 4°C by agarose beads preincubated with rabbit anti-HTT D7F7 antibody (Cell Signaling, Cat#5656). To remove the non-specific binding ...
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bioRxiv - Genetics 2024Quote: ... and then fixed at different time points (1 hour, 3 hours, 6 hours and 24 hours) before staining for DNA damage markers: ψH2AX (Cell Signaling Technology # 9718T), 53BP1 ((Novus Biologicals # NB100-904 ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed by 1× TBST 3 times and incubated with a secondary anti-rabbit-HRP or anti-mouse antibody-HRP (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were blocked with 3% BSA in PBS for 1 hour and cells were stained with VEGFR2 (Cell Signaling; C#2479; 1:200) and EEA1 (R&D Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... Apoptotic cells were identified in paraffin-embedded sections by immuno-staining with activated cleaved caspase 3 antibody (Cell Signaling Technologies, Inc., Danvers, MA). Immunohistochemistry was performed using guinea pig antiinsulin primary antibody (Fitzgerald Labs ...
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bioRxiv - Cancer Biology 2023Quote: ... OV90 and CAOV3 cells were cultured in a glass bottom 96 well plate as described and stained with Cleaved caspase 3 (CC3) antibody (Cell signaling technology: 9661T) at 1:100 dilution after 12 hours incubation with T cells for CAOV3 and 48 hours incubation for OV90 cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and Western blot performed on resultant protein as described by us.[85] Samples were probed using antibody against cleaved caspase 3 (Cell Signaling Technology, #9664) and β-actin (Santa Cruz ...
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bioRxiv - Molecular Biology 2024Quote: ... All membranes were additionally normalized for variation in loading by incubation with vinculin or glyceraldehyde-3-phosphate dehydrogenase (GAPDH, both Cell Signaling, Danvers, MA). After overnight incubation ...
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bioRxiv - Microbiology 2020Quote: ... for evaluation of histopathologic changes by H&E staining or for immunohistochemical detection of reovirus antigen (1:45,000 dilution) or the cleaved (active) form of caspase-3 (Cell Signaling, 9664; 1:300 dilution). Images were captured at a magnification of ×20 and a resolution of 0.5 μm/pixel using a high-throughput Leica SCN400 slide scanner automated digital imaging system.
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bioRxiv - Molecular Biology 2020Quote: ... phospho-eIF2α (Ser51) clone D9G8 #3398) or mouse monoclonal antibodies (eIF2α clone D-3, Santa Cruz #sc-133132, β-Actin clone E4D9Z, Cell Signaling Technology #58169 ...
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bioRxiv - Cell Biology 2020Quote: ... A polyclonal antibody against phosphorylated Tyr416 of Src and a polyclonal antibody against mouse caspase-3 were from Cell Signaling Technology (Beverly, MA, USA). α-Thrombin was purchased from Enzyme Research Laboratories (South Bend ...
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bioRxiv - Genomics 2020Quote: ... sections were blocked in 3% bovine serum albumin (BSA) and incubated over-night with antibodies recognizing Birc5 (#2808, Cell Signaling Technology, Danvers, MA, USA), Cyclin D1 (#2978 ...
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bioRxiv - Cell Biology 2021Quote: ... PFA-fixed sections were incubated with the following primary antibodies overnight at 4°C: rabbit anti-cleaved caspase 3 (Cell signaling, #9661, 1:100), mouse anti-BrdU (Dako ...