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Citations for Cell Signaling Technology :
1101 - 1150 of 2729 citations for 4' Bromo 2' fluoro 3 2 methoxyphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells were then permeabilized and blocked with 0.1% saponin/0.1% BSA in PBS before incubated with α-SARS-CoV-2 Nucleocapsid primary antibody (Cell Signaling Technology) at 1:1000 in permeabilization/blocking buffer overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody incubation was carried out overnight on a rotor at 4ºC in 100 μL antibody solution (wash buffer with 0.1% digitonin and 2 mM EDTA) containing 1 μL of αH3K27me3 Ab (Cell Signaling C36B11). Samples were then washed in dig-wash buffer (wash buffer with 0.1% digitonin) ...
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bioRxiv - Neuroscience 2024Quote: ... every 8th section of the brain was processed for double-fluorescent immunostaining for Ku80 and one of the following: Microtubule-associated protein 2 (MAP2; 8707, Cell Signaling), doublecortin (ab18723 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2 h blocking with 15% milk powder (in TBST) and overnight incubation with rabbit anti-BIP (3183, Cell signaling, 1/1000 for retina ...
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bioRxiv - Microbiology 2024Quote: ... The following antibodies were used for viral protein analysis: SARS-CoV-2 anti-Spike and anti-N protein (cat #569996 and #33336, respectively, Cell Signaling) and anti-β-actin (Sigma – cat #a2228) ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was quantified by BCA assay and 200 μg of protein was incubated with 2 μg/ml of primary antibody or with isotype control (rabbit IgG or mouse IgG isotype controls; Cell Signaling). Protein-antibody complexes were incubated overnight with constant rotation at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... at room temperature for 2 hrs in blocking buffer and treated with horseradish peroxidase (HRP) conjugated Goat anti-Rabbit IgG secondary antibody (Cell Signaling) or Goat anti-mouse IgG secondary antibody respectively for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-phospho Myosin Light Chain 2 (Ser19) (pMLC2) (#3671 from Cell Signaling Technology, 1:1000 for IB; 1:200 for IF); anti-phospho Cofilin-1 (Ser3 ...
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bioRxiv - Molecular Biology 2024Quote: ... the following antibodies were used: Cleaved caspase-3 (Casp-3, #9661, Cell Signaling, 1:50), vimentin (#PA5-142829 ...
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bioRxiv - Plant Biology 2020Quote: ... membranes were incubated with the antibody against Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® (Cell Signaling Technology, USA). As a loading control membranes were incubated with anti-tubulin antibody (Abiocode ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% milk and incubated in 2% BSA with primary antibodies to GAPDH (Cell Signaling Technology Cat# 13084, RRID:AB_2713924), Eg5 (Sigma Aldrich Cat# SAB4501650 ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then blotted overnight with anti-human phospho-Akt and anti-human phospho-Erk1/2 mAbs (Cell Signaling, MA, USA), followed by anti-rabbit or anti-mouse horseradish peroxidase(HRP)-conjugated secondary mAb (ThermoFisher scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were permeabilized with 0.1% Triton-X and stained for intracellular phospho-p44/42 Erk1/2 Thr202/Tyr204 at 1:700 dilution (Cell Signaling Technology, 4370) and p44/42 Erk1/2 at 1:700 dilution (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... and the pellet containing the DOC-insoluble material was resuspended in 100 μl SDS solubilization buffer, pH8.8 (20 mM Tris-Cl, 2 mM EDTA, 1% w/v SDS, 1X protease inhibitor cocktail (Cell Signaling Technology, 5871). The DOC-insoluble pellet was thoroughly dissolved by heating the sample to 95 °C and vortexing ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei pellets were washed twice with 200 μL of Dig-wash buffer before resuspending in 100 μL Dig-wash buffer supplemented with 2 mM EDTA and a 1:50 dilution of H3K27ac primary antibody (Cell Signaling, 8173) and incubated overnight at 4°C on an overhead rotator ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-ERK1/2 (dilution 1:2000) and rabbit α-tubulin (dilution 1:2000) all purchased from Cell Signaling Technology (Danvers, MA). The phospho-ERK1/2 expression was evaluated first using a 680-nm secondary anti-mouse antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... discs were washed 5 times for a total of 30 minutes with 0.1% PBST and incubated overnight in goat anti-mouse F(ab)’2 AlexaFluor-555 secondary antibody (Cell Signaling, 1:500). Finally ...
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bioRxiv - Plant Biology 2020Quote: ... Phosphorylated MAPKs were detected using α-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) rabbit monoclonal antibody (1:2000, Cell Signaling Technology, USA) for 1 h at room temperature in a solution of 5 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Anti-SARS-CoV-2 spike antibody (BEI Resources NR-52947) was used at 1:1000 and anti-rabbit HRP (Cell Signaling Technology) at 1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubation with the following primary antibodies: phospho-p44/42 MAPK (EER1/2) (T202/Y204) (Cell Signaling Technology, Cat. #: 9101, 1:1000), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Plant Biology 2021Quote: ... The phosphorylated MAPK3 and MAPK6 proteins were detected using polyclonal anti-phospho-p44/42 MAPK (Erk1/2, 1:1000; Cell Signaling Technology), and the detection of the total MAPK3 and 6 protein levels were by using polyclonal anti-MAPK3 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse brain lysates or cell lysates (1-2 mg) with protease inhibitor cocktail were incubated with anti-MeCP2 (Cell Signaling Technology, 3456) or anti-OGT (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-human myosin light chain 2 (D18E2) [with cross-reactivity to mouse] (catalog # 8505, RRID:AB_2728760; Cell Signaling Technology, Danvers, MA, USA), and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Vero E6 hTMPRSS2 cells infected with SARS-Cov-2 were lysed and analyzed by Western blot as done in with Rabbit Anti-SARS CoV-2 (COVID-19) Spike (Cat No. GTX135356) (Gene Tex) and Rabbit anti-Vinculin #13901 (E1E9V XP®) (Cell signaling) as loading control ...
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bioRxiv - Plant Biology 2020Quote: ... or phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (anti-phospho-TEY, #9101, 1:200, Cell Signaling Technology, Danvers, MA, USA). Membranes were washed 3 times for 5 min with TBST and incubated for 1 h with the appropriate secondary antibody – goat anti-rabbit (1:20,000 ...
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bioRxiv - Cancer Biology 2021Quote: ... MAPK activity was assessed using phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) primary antibody (1:200, rabbit, Cell Signaling Technologies #9101), total p44/42 MAPK (ERK1/2 ...
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bioRxiv - Pathology 2021Quote: ... The separated proteins were transferred onto a nitrocellulose membrane for detection with polyclonal rabbit anti-Phospho-Myosin Light Chain 2 (Ser19) antibody (Cell Signaling Technologies) or monoclonal rabbit anti-Myosin Light Chain 2 (D18E2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were spun down and washed three times with cold Flow Wash Buffer (FWB) (PBS, 2% FBS, 2mM EDTA) and incubated with primary antibody (EGFR-D1D4J, Cell Signaling Technology) diluted in FWB for 60 minutes on ice ...
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bioRxiv - Microbiology 2022Quote: ... cells were blocked with 5% milk in PBS for 1 h and then incubated with α-SARS-CoV-2 nucleocapsid antibody (1:3,000; #26369 Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein electrophoresis and Western-Blot were performed as described previously using the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology) (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... was done overnight at 4°C and was followed by incubation with anti-mouse IgG (H+L) F(ab’)2 conjugated to Alexa Fluor 647 (Cell Signaling Technology). Images were acquired with ZEISS LSM 980 laser-scanning confocal microscope and analysed using ZEN software (Carl Zeiss MicroImaging).
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bioRxiv - Immunology 2023Quote: ... Cells were then washed twice in 1 ml FACS buffer and resuspended in 50-100 μl of 1:50 diluted primary PE-conjugated antibodies against phosphorylated p38 or Erk1/2 (Cell Signaling Technology) and stained for 1 h at room temperature on a rotor ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies to EGR1, EGR3, phospho-ERK1/2, GAPDH and actin, and an inhibitor of EphA2 (dasatinib, 100 nM) were from Cell Signaling Technologies. Secondary HRP-conjugated secondary antibodies used were goat anti-rabbit (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... These steps were followed by the incubation with primary antibodies overnight at 4°C in a solution of 2% NGS in PBS 1x: chicken anti-mCherry (1:1000, HenBiotech, Portugal) and rabbit anti-NeuN (1:100, Cell Signaling, USA), rabbit anti-Iba1 (1:750 ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryos were incubated in rabbit anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody (1:200, catalog # 3678, Cell Signaling Technology) in 1% BSA/PBST overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibody incubation was performed for 1h at room temperature in phospho-p44/42 MAPK (ERK1/2) (Thr202/Thr304) antibody (#4370, 1:200, Cell Signaling Technology) or human VE-Cadherin antibody (#AF938 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1–2 hours at room temperature with the following primary antibodies: anti-E-cadherin antibody (Cell Signaling Technology, Danvers ...
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bioRxiv - Cell Biology 2023Quote: ... anti-PKM 1/2 (C103A3, #3190), anti-PKM2 (D78A4, #4053), and anti-pY849 PDGFRα/pY857PDGFRβ (C43E9, #3170S) antibodies were purchased from Cell Signaling Technology. Rabbit monoclonal anti-GLUT1 (ab115730) ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated using a 10% acrylamide SDS-PAGE gel and immunoblotted with anti-phospho-p44/42 MAPK (Erk1/2) (anti-pMAPK; Cell Signaling Technology).
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of SARS-CoV-2 nucleocapsid protein (NP) (E8R1L) fragmented mouse monoclonal antibody f-mAb (Cell Signaling Technology Inc., Massachusetts, USA) are introduced to the sensor surface after diluting in PBS and incubated for 2 hours at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... The membrane was blotted with 1:1,000 dilutions of antibodies for phospho-p44/42 MAPK (phospho-Erk1/2; Cell Signaling Technology #9101), p44/42 MAPK (Erk1/2 ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylation of MAP Kinases were detected by an antiphospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) HRP-conjugated antibody (Cell Signaling Technology) and actin was detected by HRP conjugated Anti-Plant Actin Mouse Monoclonal Antiboty (3T3 ...
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bioRxiv - Neuroscience 2024Quote: ... then permeabilized and blocked simultaneously in PBS containing 0.1% Triton-X-100 and 2% Normal Goat Serum (NGS) (Cell Signaling Technology, 5425S) for 1 hour at RT ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylated MAP kinases were detected by western blot using Phospho-p44/42 MAPK (Erk1/2; Thr-202/Tyr-204) rabbit monoclonal antibodies #9101 (Cell Signaling Technology). Blots were stained with PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Equally normalized protein samples were subjected to immunoblot analysis using Anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cat # 9101S, Cell Signaling Technology) as the primary antibody and peroxidase-conjugated goat Anti-rabbit IgG (Cat # AB205718 ...
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bioRxiv - Developmental Biology 2024Quote: ... The sections were then washed with 1X PBS (2 times, 5 minutes each) and incubated with the primary antibody (COL1A1, Cell signaling #72026S) at 1:50 dilution for 24h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then subjected to SDS-PAGE and Western blot analysis using a combination of p44/42 MAPK (Erk1/2) (3A7) Mouse mAb (Cell Signaling Technology) and Phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Genomics 2024Quote: ... Phospho-MAPK was detected using the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (clone 4370, Cell Signaling, 1:100 dilution) by the UCSF Histology and Biomarkers Core on an automated Ventana BenchMark Ultra platform ...
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bioRxiv - Molecular Biology 2024Quote: ... Plates were then incubated overnight at 4°C with primary antibodies (mouse anti-LgBIT antibody at 1:750 dilution [Promega N7100]; mouse anti-SARS-CoV-2 N antibody diluted 1:1,000 [Cell Signaling Technology 33717]) diluted in PBS + 0.1% (v/v ...