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Citations for Cell Signaling Technology :
51 - 100 of 101 citations for Recombinant Cynomolgus PDCD1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... GST tagged PTP-PEST was pulled down via immunoprecipitation using GST antibody (Cell Signaling Technology, USA). Immunoprecipitated proteins were loaded on to SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... Myc-tagged proteins were detected using myc-tag (9B11) mouse monoclonal antibody (Cell Signaling Technology #2276S) at 1:5000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... or 0.05ng/nL human CSF-1 recombinant protein (Cell Signaling Technology, 8929SC).
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bioRxiv - Immunology 2023Quote: ... recombinant ancestral S protein (S1+S2, Cell Signaling Technology; 1 µg/ml) or recombinant nucleocapsid protein (Acrobiosystems ...
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bioRxiv - Bioengineering 2023Quote: ... Nanobody proteins were confirmed by immunoblotting using anti-His-tag (2365S, Cell Signaling Technology, MA) and anti-VHH (A01860 ...
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bioRxiv - Genetics 2020Quote: ... and Myc-tagged Rrp4 proteins were detected with anti-Myc monoclonal antibody 9B11 (1:2000; Cell Signaling). 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Genetics 2022Quote: ... Myc-tagged Rrp4 proteins were detected with mouse anti-Myc monoclonal antibody 9B11 (1:2000; Cell Signaling). TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP ...
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bioRxiv - Genetics 2022Quote: ... and Myc-tagged Rrp4 proteins were detected with anti-Myc monoclonal antibody 9B11 (1:2000; Cell Signaling). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Molecular Biology 2021Quote: Mouse recombinant IFN-gamma (Ifng) was purchased from Cell Signaling Technology (catalog #5222). The typical concentration used was 10 ng/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Human recombinant IFN-gamma (IFNG) was purchased from Cell Signaling Technology (catalog #8901). The concentration used was 100 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... Membranes were incubated with goat anti-mouse or goat anti-rabbit antibody tagged with horseradish peroxidase (Cell Signaling) and immunoreactive bands were detected by enhanced chemiluminesence (GE Healthcare and Boston Bioproducts) ...
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bioRxiv - Immunology 2020Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). Flag-tagged proteins were immunoprecipitated with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2024Quote: ... Myc-tagged mCherry-PIF5 was detected using rabbit anti-Myc polyclonal antibodies (2272, Cell Signaling Technology, Danvers, MA) as the primary antibody at 1:100 dilution and donkey anti-rabbit AlexaFluor 555 antibodies (A31572 ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-HA tag and anti-His tag antibodies (Cell Signaling Technology Inc., Danvers, MA, United States).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the membrane was incubated with Rabbit His-Tag antibody (1:1000) (#12698, Cell Signaling Technology, USA) at 4 °C overnight and IRDye® 800CW Goat anti-Rabbit IgG (1:15000 ...
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bioRxiv - Biophysics 2019Quote: ... cells were treated with recombinant human TGF-β1 (10 ng/ml, Cell Signaling Technology) 59 ...
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bioRxiv - Genetics 2020Quote: ... and Myc-tagged Rrp46 and EXOSC5 proteins were detected with anti-Myc monoclonal antibody 9B11 (1:2000; Cell Signaling). As loading controls ...
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bioRxiv - Microbiology 2020Quote: ... Alexa flour 488 conjugated rabbit monoclonal His-tag was purchased from Cell Signaling Technology (Danvers, MA, USA). Fluorescein-conjugated goat anti-human IgG (#ZF-0308 ...
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bioRxiv - Molecular Biology 2019Quote: ... the cell lysates were immunoprecipitated with the monoclonal anti-his-tag antibody (Cell Signaling Technology, Inc.; USA) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... followed by detection with 1μg/mL of AF647-conjugated secondary anti-HIS antibodies (Cell Signaling, Danvers, MA) for 30 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... The protein complex was isolated by immunoprecipitation using a mouse anti-His antibody (Cell Signaling, catalog #: 2366S) followed by SDS-PAGE and Western blot analysis with a rabbit anti-GABAA receptor α1 subunit antibody (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti-His tag antibody (catalog #: 2366S) was obtained from Cell Signaling Technology (Danvers, MA).
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bioRxiv - Developmental Biology 2020Quote: ... overnight in)and probed again with fluorescent-tagged goat IgG to rabbit IgG (1:500, Cell Signaling Technology; room temperature, 1 h), both in PBS supplemented with 1% BSA and 0.3% Triton X-100 ...
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bioRxiv - Microbiology 2023Quote: ... HA- and EGFP-tagged proteins were incubated with anti-HA antibody (1: 2,000; 2999, Cell Signaling Technology, Danvers, MA, USA) or anti-EGFP antibody (1 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with Alexa Fluor 488 or Alexa Fluor 555 tagged anti-mouse antibody (1:1000, Cell Signaling Technologies) for 1hr at RT in a humid black chamber ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then incubated with the recombinant pAG-MNase (Cell Signaling, 40366; 1:33 volume) in 150 µl Perm buffer per well for an hour at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... U-2 OS cells underwent treatment with 50 ng/mL recombinant IFN-γ (Cell Signaling). The cytokine was diluted in 0.5% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein was run on an SDS PAGE and probed with anti-his antibody (Cat # 2365, Cell Signaling Technologies).
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bioRxiv - Cell Biology 2020Quote: ... The pull-down assay of ZFP982-MYC tagged protein was performed through co-Immunoprecipitation (co-IP) using MYC-Tag (9B11) antibody (#2276, Cell Signaling). For MYC-co-IP ...
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bioRxiv - Bioengineering 2019Quote: ... and stained following standard protocols previously described (Xue et al. 2011) with primary antibodies against 3xHA-tagged Nme2Cas9 (anti-HA; Cell Signaling) and mCherry (anti-RFP ...
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bioRxiv - Cancer Biology 2022Quote: Cells with stable expression of FLAG-tagged human PDE6D were lysed in 1X cell lysis buffer (#9803, Cell Signaling, Danvers, MA) and incubated with anti-FLAG M2 Affinity Gel (A2220 ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged and myc-tagged proteins were detected with rabbit anti-DYKDDDDK (FLAG) monoclonal antibody and rabbit anti-myc monoclonal antibody (Cell signaling), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... and myc- and HA-tagged proteins were immunoprecipitated with Protein A/G beads (GeneBio, 22202B-1) coated with anti-myc antibody (Cell signaling, 2276S) at a dilution of 1:250 or anti-HA antibody (Cell signaling ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were sorted for the coexpression of each component via fluorescence-activated cell sorting on a FACS ARIA II (Beckton-Dickinson) by staining with appropriate fluorescently tagged anti-Myc and anti-Flag antibody for 30 minutes at 4°C (Cell Signaling Technologies) or expression of the transgenes ...
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bioRxiv - Neuroscience 2024Quote: ... the top five recombinant p-S65-Ub antibodies or the reference antibody (Cell Signaling Technology, 62802, E2J6T) were used as capturing agents ...
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bioRxiv - Molecular Biology 2022Quote: Synchronized young adult animals expressing N-terminal HA-tagged PMK-1 were used for immunoprecipitation with anti-HA-Tag (C29F4) rabbit mAb (Cell Signaling Technology 11846) and eluted with 2x laemmli buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The prey and bait proteins were detected using anti-His (1:1000, No. #2365; Cell Signaling Technology [CST], Danvers, MA, USA) and anti-GST (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in 5% milk in PBST for 1 hour at room temperature and incubated overnight at 4°C with agitation in primary antibody at the following dilutions: rabbit anti-His (1:1,000, Cell Signaling, 2365), rabbit anti-GST (1:1,000 ...
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bioRxiv - Immunology 2021Quote: ... 96-well plates were coated with human ACE2 recombinant protein (1μg/ml, mFc tag; #83986, Cell Signaling Technology), and then blocked using PBS containing 5% skim milk for 2 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the 96 well plate was coated with human ACE2 recombinant protein (1μg/ml, mFc tag; Cell Signaling Technology). The plate was blocked with PBS containing 5% skim milk for 2 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μg/ml of recombinant antibodies and 0.2 μg/ml of the reference antibody (Cell Signaling Technology, 62802) were used ...
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bioRxiv - Genomics 2023Quote: ... The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara ...
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bioRxiv - Microbiology 2023Quote: ... The spike protein and fragments were detected by rabbit monoclonal antibodies (mAb) against the 6-His tag (12698S) from Cell Signaling Technology. The nitrocellulose (NC ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant mouse Ifng and human IFNG proteins (with carrier) were purchased from Cell Signaling Technology (catalog #39127 and 8901). They were reconstituted with sterile water at a concentration of 0.1 mg/ml ...
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bioRxiv - Genomics 2020Quote: ... rabbit anti-GATA1 (NF that was produced against recombinant N-terminal zinc finger76, rabbit anti-GAPDH (14C10, Cell Signaling) and anti-β3 integrin (sc-14009 ...
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bioRxiv - Microbiology 2021Quote: Kinase assay was performed using recombinant proteins purified from E.coli and commercially available 10X Kinase buffer (Cell Signaling Technology, #9802). Recombinant proteins (N’-His PINK1 and N’-His Ajuba ...
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bioRxiv - Plant Biology 2023Quote: ... Hemagglutinin (HA)-tagged mutant PpRopGAP1 in yeasts was detected with an anti-HA primary antibody (C29F4) of rabbit origin (Cell Signaling Technology, cat. 3724T, 5000× dilution) and an anti-rabbit-HRP (goat ...
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bioRxiv - Microbiology 2021Quote: ... The blots were incubated overnight at 4°C with the primary antibodies: His-Tag (D3I1O) XP® Rabbit mAb (1/1,000 dilution) (Cell Signaling Technology, Danvers, MA) and Myc-Tag (9B11 ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with a 1:500 dilution of Alexa Fluor® 488-labeled rabbit anti-His tag antibody (Cell Signaling Technology, Danvers, MA) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit monoclonal anti-CREB1 1:2,000 diluted in the corresponding blocking solution (5% BSA-TBST) (Cat# 9197, RRID: AB_331277, immunogen: recombinant protein specific to the amino terminus of human CREB1 protein, Cell Signaling Technology, Danvers, MA, USA); rabbit affinity isolated anti-actin 1:3,500 diluted in PBST (Cat# A2066 ...