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Citations for Cell Signaling Technology :
51 - 100 of 206 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and blocked with IHC-blocking buffer (IHC-PBS-T with 10% normal goat serum (Cell Signaling Technology)) for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... resuspended in 200ul of blocking buffer and conjugated with 4ugr of anti-H2AK119ub antibody (Cell Signaling, 8240S) for 3h at 4C in a rotating platform ...
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bioRxiv - Bioengineering 2023Quote: ... SARS-CoV-2 Spike RBD-ACE2 Blocking Antibody Detection ELISA Kit was obtained from Cell Signaling Technologies.
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bioRxiv - Microbiology 2023Quote: ... then incubated with secondary antibody for 1 hour (1:10000 dilution in blocking buffer, Cell Signaling, 7074S). The horse-radish peroxidase conjugated to the secondary antibody was detected by incubating the membrane with 2 mL chemiluminescence substrate (Bio-Rad ...
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bioRxiv - Systems Biology 2022Quote: ... Peptides were then incubated with 4 µl of K-GG antibody bead-conjugate (Cell Signaling Technology, PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG ...
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bioRxiv - Cancer Biology 2022Quote: ... TMT10 labeled Peptides with acetylated K were enriched with Acetyl-Lysine Motif kit (Cell Signaling Technology) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Dry peptides were gently resuspended in 1.4 ml 1X immunoaffinity purification (IAP) buffer (Cell Signaling Technology) and cleared by centrifugation for 5 min at 10,000 ref at 4C ...
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bioRxiv - Pathology 2023Quote: ... and then succinylated peptides were enriched using the PTMScan Succinyl-Lysine Motif Kit (Cell Signaling Technologies) as previously described but with slight adaptations (also see Supplemental Appendix 1 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were incubated with primary antibodies in blocking buffer: rabbit anti-GAPDH (1:5000; Cell Signaling Technology #2118) and mouse anti-Nluc (0.5 μg·ml−1 ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked with a blocking buffer of 5% non-fat milk powder (Cell Signaling Technology, Danvers, MA) in phosphate buffered saline with 0.05% tween-20 (PBS-T ...
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bioRxiv - Systems Biology 2021Quote: ... The secondary antibodies were diluted in the blocking solution as follows: anti-mouse Alexa 647 (Cell signaling 4410S) 1/1000 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked using Odyssey blocking buffer and incubated in primary antibodies overnight (αTubulin, 1:1000, Cell Signaling; SIRT3 ...
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bioRxiv - Immunology 2023Quote: ... Blocking was followed by incubation with rabbit monoclonal anti-cFos primary antibody (1:1000 dilution, Cell Signaling 2250S) or rabbit polyclonal anti-Fluoro-Gold primary antibody (1:1000 Fluorochrome ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissue sections were then incubated with a blocking solution containing 5% goat serum (Cell Signaling Technology; 5425) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were added at 1:250 dilution in 1X Animal-Free Blocking Solution (Cell Signaling Technology, 15019L) to the apical side of each Transwell® for an overnight incubation at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Mono-methyarginine peptides were enriched by a PTMscan Mono-Methyl Arginine Motif [mme-RG] Kit (Cell Signaling) according to the manufacturer’s protocol followed by strong cation exchange (SCX ...
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bioRxiv - Cancer Biology 2022Quote: ... the peptides were lyophilized and resuspended in immunoaffinity purification (IAP) buffer (PTMScan, Cell Signaling Technology, Beverly, MA). The supernatant containing pure peptides was centrifuged for 5 minutes at 10,000 × g at 4°C in a microcentrifuge ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were purified for the K-GG modification using the K-GG motif antibody kit (Cell Signaling). Eluted peptides were desalted and concentrated with PerfectPure C18 tips following with LC-MS/MS analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were used at 1:2000 dilution in blocking buffer: Anti-rabbit IgG HRP-linked (7074S, Cell Signaling) and anti-mouse IgG HRP-linked (7076 ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was then incubated overnight in rabbit anti-ΔFosB (1:2000 in blocking solution, Cell Signaling, Danvers, MA) overnight ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... Blocking was followed by overnight incubation at 4 ºC with rabbit anti-cleaved caspase 3 antibody (Cell Signaling 9661) at 1:400 dilution in antibody dilution buffer (1% BSA ...
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bioRxiv - Genetics 2020Quote: ... followed by blocking with milk or BSA and antibody labelling with specific antibodies to phospho-eIF2α (Ser51) (Cell Signaling), puromycin (Merck Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... Blocking buffer was removed and replaced with primary antibody solution (rabbit α-HA (C29F4, 1:1000, Cell Signaling Technology) and mouse α-HSP70 (5A5 ...
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bioRxiv - Neuroscience 2023Quote: ... blots were incubated in blocking buffer for 1 hour and probed using primaries Lamin B/C (Cell Signaling, 4777S), STAT3 (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were first incubated with blocking and permeabilization buffer (1X PBS / 5% normal goat serum (Cell Signaling, cat #5425) / 0.3% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated overnight at 4 °C with primary antibody in the same solution used for blocking (Cell Signaling Technology rabbit antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then incubated overnight in blocking solution with antibodies recognizing CD45 (Cell Signaling cat. no 70257S; 1:200) or CD3 (Abcam cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then washed twice with PBS and incubated with blocking solution (10% goat serum (Cell Signaling; Danvers, MA) in PBS supplemented with 0.1% Tween-20 ...
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bioRxiv - Developmental Biology 2024Quote: ... the blocking solution was drained and replaced with primary antibody: Cleaved Caspase-3 (1:250, Cell Signaling cat#9661T), or αPhospho-histone H3 (Ser10 ...
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bioRxiv - Microbiology 2019Quote: Peptide samples were subjected to ubiquitin remnant immunoaffinity purification with 31 μg of ubiquitin remnant antibody (Cell Signaling). Peptides were lyophilized for two days to remove TFA in the elution ...
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bioRxiv - Genetics 2019Quote: Sample preparation and enrichment of monomethyl arginine peptides were conducted using PTMScan[mme-RG] kit (Cell Signaling Technology) according to manufacturer’s protocols with the following modifications ...
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bioRxiv - Cancer Biology 2021Quote: ... Bound PRKAR1a or HK2 were detected by incubation with rabbit anti- PRKAR1a antibody (Cell Signaling; 1:2000 in blocking buffer) or rabbit anti-HK2 antibody (Cell Signaling ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked in Odyssey Blocking Buffer (Licor) and probed with the following antibodies: pERK (Cell Signaling Technology, 1:1000), total ERK (Cell Signaling Technology ...
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bioRxiv - Immunology 2022Quote: ... Normal horse serum was used for non-specific blocking and Rabbit anti-mouse CD8α (clone D4W27, Cell Signaling Technology®) was used as detection antibody ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked with Odyssey blocking buffer (TBS; Li-Cor) and incubated with: i) monoclonal anti-STAT3 (12640S, 1:1,000; Cell Signaling); ii ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... Neurons were then incubated with blocking solution overnight with primary antibodies: rabbit anti-pCREB (Cell Signaling, catalog #9198, 1:1000), and mouse anti-CaMKIIα 6G9 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... at 1:1,000 dilution in blocking buffer or glucose 6-phosphate dehydrogenase (G6PDH) antibodies (8866, Cell Signaling Technology, 1:1,000). Immunoreactive species were detected with antibodies conjugated with horseradish peroxidase (715-035-150 ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were incubated overnight in primary antibody diluted in blocking buffer (1:1000 anti-cleaved caspase-3 Cell Signaling Technologies #9664 ...
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bioRxiv - Plant Biology 2019Quote: ... Detection of phosphorylated proteins was performed in blocking solution (5% BSA, 0.1% [v/v] Tween-20) with anti-phosphothreonine antibodies (9381, Cell Signaling) and with anti-rabbit-HRP (W4011 ...
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bioRxiv - Cancer Biology 2021Quote: ... Bound PRKAR1a or GSK3b were detected by incubation with rabbit anti- PRKAR1a antibody (Cell Signaling; 1:2000 in blocking buffer) or mouse anti-GSK3b antibody (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... and blocked in blocking buffer before incubation with 1:500 dilution of rabbit anti-HA primary (3725; Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... blots were incubated in blocking buffer (5%(w/v) milk powder (nonfat dry milk, Cell Signaling Technology, Danvers, MA, USA)) in TBST containing 25 mM Tris base ...
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bioRxiv - Cell Biology 2023Quote: ... and staining was performed after blocking with 5% normal goat serum with anti-Insulin (Linco) and anti-Glucagon (Cell Signaling) antibodies using established protocols ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were resuspended in 500µL blocking buffer and mixed with 7.5µg antibody (NUT monoclonal rabbit antibody (Cell Signaling Technology, #3625S), or H3K27Ac monoclonal rabbit antibody (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: Primary antibodies were diluted 1:1000 in blocking buffer and included rabbit α-TOMM20 (clone D8T4N, Cell Signaling Technologies #4240), mouse α-MT-CO1 (clone 1D6E1A8 ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary and secondary antibodies were diluted in blocking buffer and used 1:100 for anti-FoxO1 (rabbit; Cell Signaling, 2880), 1:100 for anti-ESRRB (mouse ...
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bioRxiv - Physiology 2020Quote: Ubiquitinated peptides were purified using immunoaffinity purification with the diglycine-lysine ubiquitin remnant motif antibody (Cell Signaling Technology, PTMScan) cross-linked to Protein A agarose beads ...
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bioRxiv - Cancer Biology 2020Quote: Phosphotyrosine peptides were enriched using a PhosphoScan Kit (p-Tyr-100 and p-Tyr-1000, Cell Signaling, Danvers, MA). The lyophilized peptide was dissolved in IAP buffer (50 mM MOPS ...
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bioRxiv - Immunology 2019Quote: ... Membranes were blocked for 1 h at RT in Odessy blocking buffer (Licor) and incubated overnight at 4°C with the following antibodies: IRF3 (Cell Signaling) 1:1000 ...
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bioRxiv - Developmental Biology 2019Quote: ... Western blots were then blocked with LI-COR blocking buffer and incubated overnight with primary antibodies anti-STAT3 (Cell Signaling, #4904), anti-pSTAT3 (Cell Signaling ...