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Citations for Cell Signaling Technology :
51 - 100 of 1652 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Blots were blocked in 5% bovine serum albumin in tris-buffered saline with 0.1% Tween 20 (TBS-T) and incubated overnight at 4°C with rabbit anti-phospo-Ser505-Akt (Cell Signaling Technology 4054) or mouse anti-α-Tubulin (DSHB 12G10 ...
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bioRxiv - Neuroscience 2024Quote: ... sheared chromatin was centrifuged at 16,000 x g for 20 minutes at 4 °C and dissolved in 1x ChIP buffer (Cell Signaling Technology, #57976). 2% input was taken and chromatin was incubated with antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 20% swine serum in PBS and then incubated with primary antibodies overnight at 4 °C (γH2AX ((#9718, Cell Signaling Technologies). The following day ...
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bioRxiv - Cancer Biology 2024Quote: Immunoblotting performed as previously described(22).Immunoblotting was performed with the following antibodies: ARID1A (Cell Signaling, 12354, 1:1000), ARID1B (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit polyclonal (5483, Lot 15, Cell Signaling); cingulin ...
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bioRxiv - Biochemistry 2024Quote: ... per sample were first conjugated with 4 μg (10 μL) mouse anti-myc-tag antibody (Cell Signaling, 9B11) for one hour at RT in a rotating wheel ...
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bioRxiv - Immunology 2020Quote: Cell lysates from 5-10 × 106 cells were generated using CHAPS Cell Extract Buffer (Cell Signaling Technology) supplemented with 1X Halt protease inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C in 5% BSA/TTBS containing rabbit anti-STING (0.058 µg/ml, Cat. # 13647, RRID: AB_2732796; Cell Signaling Technology), anti-phospho-TMEM173/STING S366 (1 µg/ml ...
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bioRxiv - Physiology 2023Quote: ... blocked with 5% goat serum and incubated overnight at 4 °C with anti-TMEM16A (Cell Signaling, Rabbit D1M9Q 1:100) and CD31 (Abcam ...
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bioRxiv - Genomics 2021Quote: ... Nuclear stain solution was prepared for 4 slides at a time: 20 µLSYTO13 (Nanostring) and 180 µL 1x TBS (Cell Signaling Technologies, 12498S) for a total volume of 200 µL/slide ...
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bioRxiv - Molecular Biology 2022Quote: ... The blocked membrane was incubated overnight at 4°C with primary antibodies diluted in 1x Tris buffered saline with Tween-20 (TBST, Cell Signaling Technology: 9997S). The membrane was washed twice using TBST ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for 20 hours with a protein complex of anti-HA antibody (Cell signaling technology, C29F4) and Sera-Mag SpeedBeads Protein A/G (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid-bound protein was run on 4-20% SDS-PAGE gel and immunoblotted with His-tag antibody (rabbit monoclonal, Cell Signaling Technology, 2365). Blots were visualized on a Li-Cor Odyssey imaging system ...
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bioRxiv - Immunology 2024Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20 μM EDTA + 4 μM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification.
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bioRxiv - Molecular Biology 2021Quote: ... washed three times with 1x PBS and incubated with 5 % horse serum/1xPBS for 1 h at 20 °C before incubating with the following antibodies (Cell Signaling Laboratories): anti-calnexin (clone C5C9) ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1% Tween-20 in PBS) for at least 1 h and then incubated in 1:100 anti-pSmad1/5/9 (Cell Signaling, #13820) in FBS-PBST at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... blocked with TBST (1x Tris buffered saline with 0.1% Tween 20) supplemented with 5% (wt/vol) BSA for 1 hr and blotted with PU.1 (9G7) (1:1000, Cell Signaling #2258) at 4°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were added overnight in 5% milk in TBS + 0.1% tween-20 (TBST): huntingtin (Clone D7F7, Cell Signaling 5656, 1:1,000), beta-actin (Clone 13E5 ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% skim milk in PBS containing 0.05% Tween 20 and then incubated with rabbit anti-RhoA (1:1000, Cell Signaling Technology, 67B9), rabbit anti- Pten (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... the blots were washed with PBS supplemented with 0.1% Tween 20 four times for 5 min before staining with the secondary antibody (7076, Cell Signaling; ab216773, Abcam) in blocking buffer with 0.1% Tween 20 for 1h at room temperature ...
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bioRxiv - Genetics 2020Quote: ... containing 15% Fetal Bovine Serum (Cell Signaling Technologies), 1000U/mL Leukemia Inhibitory Factor (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were blocked for 1 h at room temperature with 5% skim milk and then incubated overnight at 4°C with appropriate primary antibody dilution: anti-STAT1 (1:1000, Cell Signaling), anti-pSTAT1 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... overnight at 4°C and secondary HRP-conjugated anti-rabbit IgG (1:2,000 in 5% nonfat dry milk; Cell Signaling Technology) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was blocked for 1h with 5% BSA in TBS-T followed by overnight incubation at 4°C with antibody (Drosha 1:1000; D28B1, Cell Signaling, in 5 %BSA ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were blocked with 3% BSA in PBS overnight at 4°C and then incubated with a rabbit phospho-SMAD1/5/9 antibody at 1:400 (D5B10, Cell Signaling) and a mouse anti-YAP antibody at 1:200 (63.7 ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref: 901503; anti-H3K4me3 Cell Signaling, ref ...
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bioRxiv - Neuroscience 2023Quote: ... immunoblots were probed for anti-rabbit acetylated histone 3 at lysine 9 and histone 4 at lysine 5 (ac-H3K9 and ac-H4K5 respectively, Cell Signaling), anti-rabbit LC3B (Abcam) ...
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bioRxiv - Molecular Biology 2023Quote: ... washed twice in PBS and after centrifugation at 250 x g for 5 min at 4°C resuspended in cell lysing buffer (Cell Signaling Cat# 9803 ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibody incubation of the sample was carried out overnight at 4°C with the following primary antibody dilutions: rabbit anti-pSMAD1/5/9 1:200 (mAb #13820, Cell signaling, (Genikhovich et al. ...
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bioRxiv - Neuroscience 2024Quote: ... Revert stain was then removed and then membranes were blocked in 5% BSA for 2 hours at RT and then incubated overnight at 4°C in either anti-NLRP3 antibody (15101S, 1:1000 in 5% BSA, Cell Signaling), anti-NLRC4 antibody (12421 ...
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bioRxiv - Genomics 2022Quote: ... H3K27me3 immunoprecipitation was performed on 4 million cells with 10 µL of H3K27me3 antibody (Cell Signaling, C36B11 Lot #19). H3K9me3 immunoprecipitation was performed on 3 million cells with 8 µL of H3K9me3 antibody (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... 4°C for 10 mins and the obtained supernatant was incubated overnight with anti-V5 (#13202, Cell Signaling Technology) and Protein A-Sepharose beads at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with 200 μl of 1XDPBS + 1% BSA + 0.1% Tween-20 containing 4 μl of Alexa Fluor 488-conjugated phospho-histone H3 (Ser10) antibody (Cell Signaling Technologies, 1:50 dilution) for 90 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants (Triton X-100 insoluble fraction) were collected and analyzed on 4-20% SDS-PAGE with anti-ubiquitin (Cell Signaling Technologies P4D1, #3936) and anti-Ref(2)P/p62 (Abcam #178840 ...
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bioRxiv - Immunology 2024Quote: ... An equal volume of 2× STOP Buffer (Buffer 2 plus 20 µM EDTA plus 4 µM EGTA) and 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. no. 29987) were added ...
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bioRxiv - Cancer Biology 2023Quote: ... then probed overnight at 4°C with primary antibodies diluted in a 5% BSA/ 1X TBS-0.1% Tween 20 (TBS-T) solution using either Vinculin (E1E9V) (Cell Signaling, Cat. No. 13901) diluted 1:2000 ...
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bioRxiv - Bioengineering 2022Quote: ... The membranes were blocked with PBS with 0.1% Tween-20 (PBST) with 5% Blotto for one hour and then incubated with antibodies to phospho-p90RSK (Thr359/Ser363; #9344; Cell Signaling Technology, Inc.) and RSK1/RSK2/RSK3 (32D7 ...
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bioRxiv - Biochemistry 2024Quote: ... was first incubated in the chamber for 10 minutes followed by the addition of a biotinylated EphA2 or GFP antibody for 20 minutes (Cell Signaling and Rockland Immunochemical Inc. ...
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bioRxiv - Microbiology 2024Quote: For the identification of HSF2 association on specific regions of the KSHV ORF50 promoter 2μL of antibodies against HSF2 (SFI58)(22) and as control normal Rabbit IgG (#2729, Cell Signaling Technology) were used ...
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bioRxiv - Microbiology 2024Quote: ... LumiGLO @ Reagent (20×) and 20× peroxide (Cell Signaling Technology, Danvers, MA, USA) were used as substrates for signaling using a Lumino Image Analyzer LAS2000 (Fujifilm ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858), S6 (Cell Signaling 2217) ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated overnight at 4°C with a 1:1000 dilution of rabbit anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies) in block ...
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C with the following primary antibodies diluted 1:1000 in 5% BSA/TBST: anti-pro-IL-1β (#12703, Cell Signaling Technology) and anti-NLRP3 (AG-20B-0014-C100 ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... Astrocyte activation to a neurotoxic phenotype was induced in cells at 4-5 DIV by incubation with TNFα (25 ng/ml; Cell Signaling Technology), IL-1α (3 ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... + 5% milk and then incubated overnight at 4°C with the following primary antibodies in TBS+5% milk: anti-RPB1 (1:1000; Cell Signaling, 14958), anti-EZH2 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% skim milk and probed with primary antibodies overnight at 4°C: either rabbit monoclonal anti-TET2 (Cell Signaling Technology) or monoclonal mouse anti-GAPDH (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then cut and incubated with different primary antibodies diluted in 5% milk at 4°C overnight: αH3K27me3 (Cell Signaling C36B11) was used at 1:5000 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... Magnetic beads were allowed to bind with 5 μg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) by incubating for 8 hours at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitation was carried out overnight at 4 °C using 5 μl of rabbit polyclonal FosB antibody (2251S, lot 3, Cell Signaling Technology) or 2 μg of rabbit polyclonal IgG isotype (3900S ...
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bioRxiv - Physiology 2021Quote: ... which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST) and incubated with rabbit anti-pAkt antibody (Cell Signaling Technology, 4060S, 1:1,000 dilution) or rabbit anti-AKT antibody (Cell Signaling Technology 9272S ...