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Citations for Cell Signaling Technology :
51 - 100 of 102 citations for Brucella Blood Agar with Hemin and Vitamin K 15x100mm plate 26ml fill since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Protein lysates were incubated overnight at 4 °C with rabbit acetyl-lysine (Ac-K, #9441) from Cell Signaling Technology. Protein densitometry was measured using Image J software (National Institutes of Health ...
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bioRxiv - Biochemistry 2022Quote: ... DiGly peptide enrichment was performed using the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology (CST)) ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... minced and incubated with 5 ml 1x Red Blood Cell Lysis Buffer (Cell Signaling Technology) for 5 min with manual shaking ...
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bioRxiv - Systems Biology 2020Quote: ... DiGly remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-ɛ-GG) Kit (Cell Signaling Technology (CST)) ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatants (pH ~7.5) were incubated with the washed PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) (Cell Signaling Technology) immunoaffinity magnetic beads for 2 hours at 4°C with gentle end-over-end rotation ...
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bioRxiv - Cancer Biology 2020Quote: ... Ubiquitinated peptides were enriched by immunoaffinity purification using the PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology, 5562) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... quantified with Quantitative Colorimetric Peptide Assay (Thermo Fischer Scientific) and dried on Speed-Vacuum before enrichment with Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling). After Sep Pak desalting ...
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bioRxiv - Cell Biology 2020Quote: ... Galectin-3 (#87985, IF: 1:400) and Na/K-ATPase α1 (#23565, IF: 1:100) antibodies were purchased from Cell Signaling Technology. Anti-beta-actin antibody (A2228 ...
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bioRxiv - Neuroscience 2023Quote: ... Enrichment for ubiquitinated peptides was conducted using a PTM Scan® Ubiquitin Remnant Motif [K-ε-GG] kit (Cell Signaling, Danvers, MA) following the vendor’s recommended procedures ...
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bioRxiv - Molecular Biology 2024Quote: ... DiGly-modified peptides were enriched by immunoprecipitation using PTMScan® ubiquitin remnant motif (K-Ɛ-GG) antibody bead conjugate (Cell Signaling Technology), essentially according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Immunocapture of GG-modified peptides was then performed using the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The enrichment of di-Gly peptides was performed using PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-GG) Kit (Cell Signaling Technology) as previously described99 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were alkylated and digested with trypsin at 37°C overnight and ubiquitinated peptides were enriched with PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology). After Sep Pak desalting ...
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bioRxiv - Biochemistry 2021Quote: ... Magnetic beads for all experiments come from PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology, Kit #59322), along with 1X HS IAP Bind Buffer #1 and 1X HS IAP Wash Buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... The rest of the eluate was dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology) as described in the manual ...
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bioRxiv - Systems Biology 2024Quote: The enrichment of lysine acetylated peptides was performed using a PTMScan HS Acetyl-Lysine Motif [Ac-K] Kit (Cell Signaling Technology, Cat #50071) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were later enriched using di-Gly antibody immunoprecipitation using the PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling catalog# 5562). Post immunoprecipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were dried by lyophilization and resuspended in IAP buffer from PTMScan HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling #59322). K-GG containing heavy reference peptides were pooled and diluted into the IAP buffer resuspension fat a final concentration of 1 fmol per peptide ...
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bioRxiv - Biochemistry 2021Quote: Agarose beads used in all antibody comparisons were from the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology, Kit #5562). Preparation of agarose beads included cross-linking as described previously 15 ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein was extracted with RIPA buffer and processed for acetylomic mass spectrometry as described previously55 using PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology #46784).
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bioRxiv - Molecular Biology 2022Quote: ... The following steps were performed according to the manual of the PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling #59322). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... The rest was split into 8 aliquots dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology #46784) as described in the manual and peptides were eluted twice with 200µL of 50% acetonitrile (ACN) ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane parts were probed for 18 h with anti-hsTRPM2 antibody from rabbit (Novus #nb500-241 at 1:50000 dilution) and anti-Na+/K+-ATPase antibody from rabbit (Cell Signaling #3010 at 1:1000 dilution), respectively ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plates were then incubated with rabbit-anti-LC3B (Cell Signaling Technologies, Danvers, MA) antibodies in Cell Staining Buffer for 2 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed then 100 uL of secondary anti-mouse (Cell Signaling Technology) (1:2,000 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... Plates were incubated with primary antibodies rabbit anti-ERK1/2 (1/500, Cell Signaling 4695), rabbit anti-p-ERK1/2 (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... 80-90% confluent plates were supplemented with 4µg/ml of MMC in DMSO (Cell Signaling Technology) for 4-6 hours at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 80-90% confluent plates were supplemented with 4µg/ml of MMC in DMSO (Cell Signaling Technology) for 4-6 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: Cells in plates washed twice with ice-cold PBS and lysed in RIPA buffer (Cell Signaling #9806S) with a cocktail of protease inhibitors (Sigma-Aldrich # S8830) ...
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bioRxiv - Immunology 2021Quote: ... 96-well plates were coated with human ACE2 recombinant protein (1μg/ml, mFc tag; #83986, Cell Signaling Technology), and then blocked using PBS containing 5% skim milk for 2 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Microlon ELISA Plates were coated with antibody against VDR (anti-VDR antibody) (Cell Signaling Technology, Boston, MA, USA) overnight at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... the 96 well plate was coated with human ACE2 recombinant protein (1μg/ml, mFc tag; Cell Signaling Technology). The plate was blocked with PBS containing 5% skim milk for 2 h at RT ...
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bioRxiv - Cell Biology 2021Quote: Cells cultured on 6-well plates were stained using SA-β-gal activity assay kit (Cell Signaling Technology) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... Plates were then incubated overnight at 4°C with primary antibodies (rabbit anti-IRF3 antibody [Cell Signaling Technology 11904S] ...
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bioRxiv - Cell Biology 2024Quote: Senescence associated β-galactosidase was measured in cell cultures using a plate-based fluorescent assay (Cell Signaling, 23833). In tissues β-galactosidase activity at pH 6 was determined using either Senescence β-Galactosidase Staining Kit (cell signaling ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell viability was measured from the cells growing in 96-well plate using XTT assay kit (Cell Signaling Technology) following manufacturer’s instruction using BioTek Synergy microplate reader.
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bioRxiv - Neuroscience 2022Quote: ... The plates were washed 3x with PBST and incubated overnight at 4°C under agitation with rabbit anti-phosphorylated TRKB (Cell Signaling, either #4168 against pY816 or #4619 against pY515 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The plates were incubated overnight at 4°C with rabbit anti-HA antibody (1:1000, catalog #3724S, Cell Signaling, Danvers, MA) diluted in 5% bovine serum albumin ...
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bioRxiv - Neuroscience 2020Quote: ... 5 days after irradiation the plates were assayed for senescence via the Senescence β-galactosidase Cell Staining Kit (Cell Signaling, Catalog # 9860) and the staining was performed as per manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: Cell death was induced by treating cells seeded on fibronectin-coated 96 well plates with varying concentrations of staurosporine (Cell Signaling Technology), FasL (PeproTech) ...
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bioRxiv - Genetics 2021Quote: MCF7 cells were grown in 6 well plates for Western blots and were washed with cold PBS twice and scraped in RIPA buffer (Cell signaling, USA). Protein concentration in each sample was measured through Bradford assay using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: ... The plates were then washed 3 times with PBST before adding 100 μl of TMB substrate solution (Cell Signaling Technology, USA, Catalog: 7004S) to the plates after aspirating out the PBST and was incubated for 25 minutes at room temperature on a plate shaker ...
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bioRxiv - Bioengineering 2021Quote: ... A2 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1β (30 ng/ml, Cell Signaling Technology, 8900SF) and TNF-α (30 ng/ml ...
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bioRxiv - Molecular Biology 2024Quote: Senescence-associated beta-galactosidase in HSVSMC lysates was quantified using the Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) (Cell signaling Technology). Measurement was done on cell lysates with the same total protein concentration ...
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bioRxiv - Neuroscience 2024Quote: ... 96-well Meso Scale Discovery assay plates (MSD, L15XA-6) were coated with 30 μl of pS65-Ub capturing antibody (Cell Signaling Technology, 62802) with a final concentration of 1 µg/ml in 200 mM sodium carbonate coating buffer (pH 9.7 ...
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bioRxiv - Cancer Biology 2023Quote: ... OV90 and CAOV3 cells were cultured in a glass bottom 96 well plate as described and stained with Cleaved caspase 3 (CC3) antibody (Cell signaling technology: 9661T) at 1:100 dilution after 12 hours incubation with T cells for CAOV3 and 48 hours incubation for OV90 cells ...
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bioRxiv - Cancer Biology 2024Quote: Adherent cells were washed in 1x PBS and lysed on the plate by the addition of complete RIPA buffer with protease (Cell Signaling Technology #5871S) and phosphatase (Cell Signaling Technologies #5870S ...
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bioRxiv - Neuroscience 2024Quote: ... the 96-well MSD plates (Meso Scale Diagnostics, L15XA) were coated with 1 µg/mL p-S65-Ub capturing antibody (Cell Signaling Technology, 62802), blocked with 1% BSA in TBST ...
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bioRxiv - Cell Biology 2020Quote: SMC were seeded at a density of 75000/well in 6 well plates and cultured in FGM for 48 h prior to fixation and staining using a commercial β-galactosidase assay (Cell Signaling Technologies #9860) as described previously (41) ...
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bioRxiv - Cancer Biology 2023Quote: IMR90 cells seeded on the plate were fixed and stained for SA-β-gal activity with the senescence β-Galactosidase Staining Kit according to the protocol (Cell Signaling, #9860S).