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• The autism-associated gene SYNGAP1 regulates human cortical neurogenesis

  Marcella Birtele, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Rabbit anti-SYNGAP1 (5539, Cell Signaling Technology) was diluted at a concentration of 1:200 in 0.3%TritonX-100 (Sigma ...
• GeneMAP: A discovery platform for metabolic gene function

  Artem Khan, et al., bioRxiv - Genomics 2023

  Quote: ... 1:3,000 for Western blot) and anti-rabbit IgG–HRP (7074S, 1:3,000 for Western blot) were obtained from Cell Signaling. Antibodies for immunofluorescence staining were goat anti-mouse Alexa Fluor 488 (A11029 ...
• Targeted protein degradation via intramolecular bivalent glues

  Oliver Hsia, et al., bioRxiv - Biochemistry 2023

  Quote: ... HRP anti-mouse IgG (7076, Cell Signaling Technology), IRDye® 680RD anti-mouse (no ...
• High fusion and cytopathy of SARS-CoV-2 variant B.1.640.1

  William Bolland, et al., bioRxiv - Microbiology 2023

  Quote: ... and rabbit anti-cleaved caspase-3 (D175; Cell Signaling Technology) and imaged using the LSM-700 confocal microscope (Zeiss).
• HP1 deficiency results in De-Repression of Endogenous Retroviruses and Induction of Neurodegeneration via Complement

  A.G. Newman, et al., bioRxiv - Neuroscience 2023

  Quote: ... 1.5μl of antibody (mouse anti-myc 9B11, Cell Signaling or goat anti-GFP, Rockland) was incubated per sample on a rocker for 2 hours at 4°C ...
• Single intravitreal administration of a tetravalent siRNA exhibits robust and efficient gene silencing in rodent and swine photoreceptors

  Shun-Yun Cheng, et al., bioRxiv - Neuroscience 2023

  Quote: ... Antibodies used for protein level detections are: mouse anti b-actin (1:1,000; Cell signaling, Cat#3700), rabbit anti HTT (1:1,000 ...
• Single intravitreal administration of a tetravalent siRNA exhibits robust and efficient gene silencing in rodent and swine photoreceptors

  Shun-Yun Cheng, et al., bioRxiv - Neuroscience 2023

  Quote: ... VGLUT2 (1:1,000; Cell signaling, Cat#71555).
• Molecular definition of distinct active zone protein machineries for Ca²⁺ channel clustering and synaptic vesicle priming

  Javier Emperador-Melero, et al., bioRxiv - Neuroscience 2023

  Quote: ... and mouse anti-LAR (A156, 1:500, clone E9B9S from Cell signaling). The secondary antibodies were peroxidase-conjugated goat anti-mouse IgG (S52 ...
• Acetylation-dependent coupling between G6PD activity and apoptotic signaling

  Fang Wu, et al., bioRxiv - Biochemistry 2023

  Quote: ... anti-Fyn (Cell Signaling Technology, #4023; 1:1000), anti-Flag (Sigma-Aldrich ...
• CelltypeR: A flow cytometry pipeline to annotate, characterize and isolate single cells from brain organoids

  Rhalena A. Thomas, et al., bioRxiv - Neuroscience 2023

  Quote: ... Rabbit anti-PDGFRa (Cell Signaling, 3174) was added post-fixation at a dilution of 1:200 and incubated overnight at 4°C ...
• NUDC is critical for rod photoreceptor function, maintenance, and survival

  Mary Anne Garner, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-arrestin (Cell Signaling Technologies [CST], 1:500); anti-transducin (Abcam ...
• GeneMAP: A discovery platform for metabolic gene function

  Artem Khan, et al., bioRxiv - Genomics 2023

  Quote: Antibodies against HA-Tag (3724S, 1:200 for immunofluorescence), citrate synthase (14309S, 1:1,000 for Western blot) were from Cell Signaling Technology; SLC25A12 (ab200201 ...
• Nonviral base editing of KCNJ13 mutation preserves vision in an inherited retinal channelopathy

  Meha Kabra, et al., bioRxiv - Genetics 2023

  Quote: ... GFP mouse monoclonal primary antibody (Cell Signaling#2955, 1:250) was used to enhance the Kir7.1 protein expression for its detection in the cells ...
• ARHGAP18-ezrin functions as an autoregulatory module for RhoA in the assembly of distinct actin-based structures

  Andrew T. Lombardo, et al., bioRxiv - Cell Biology 2023

  Quote: ... Rabbit anti-RhoA (Cell Signaling Technology; catalog no. 2117) was used at 1:500 (western blot) ...
• Genetic Diversity Modulates The Physical And Transcriptomic Response Of Skeletal Muscle To Simulated Microgravity

  Yasmina Zeineddine, et al., bioRxiv - Bioengineering 2023

  Quote: ... as well as 4EBP1 (4E-binding protein 1) (T37/46, Cell Signaling Technology). Primary antibodies were diluted at a 1:1000 ratio ...
• Genetic ablation of dentate hilar somatostatin-positive GABAergic interneurons is sufficient to induce cognitive impairment

  Rajasekar Nagarajan, et al., bioRxiv - Neuroscience 2023

  Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, catalog # 7074). Then the membranes were washed with TBST four times ...
• Mechano-signaling of prostate tumor initiating cells facilitates their tropism to stiff metastatic niche

  Lanpeng Chen, et al., bioRxiv - Cancer Biology 2023

  Quote: Protein samples were collected by lysing cells with lysis buffer containing phenylmethanesulfonyl fluoride (Cell Signaling Technology) and were separated by SDS-PAGE (Bio-Rad) ...
• Kras loss of heterozygosity promotes MAPK dependent pancreatic ductal adenocarcinoma and induces therapeutic sensitivity

  Sigrid K. Fey, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Primary antibodies used for immunohistochemistry were as follows: phospho-ERK1/2 (1:400, Cell Signaling Technologies #9101), cMYC (1:200 Abcam #ab32072) ...
• Mechano-signaling of prostate tumor initiating cells facilitates their tropism to stiff metastatic niche

  Lanpeng Chen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... After treating with enhanced chemoluminescence substrate mixture (Cell Signaling Technology), blots were scanned with ChemiDoc XRS+ System (Bio-rad) ...
• PRDM6 promotes medulloblastoma by repressing chromatin accessibility and altering gene expression

  Christin Schmidt, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Digestion was stopped by adding 1× stop buffer (digitonin solution, RNase A, and 50 pg S. cerevisiae spike-in DNA (Cell Signaling Technology) for normalization ...
• Modulation of Viability, Proliferation, and Stemness by Rosmarinic Acid in Medulloblastoma Cells: Involvement of HDACs and EGFR

  Alice Laschuk Herlinger, et al., bioRxiv - Cancer Biology 2023

  Quote: ... EGFR (1:1,000; Cell Signaling, #4267), pERK1/2 (1:1,000 ...
• Modulation of Viability, Proliferation, and Stemness by Rosmarinic Acid in Medulloblastoma Cells: Involvement of HDACs and EGFR

  Alice Laschuk Herlinger, et al., bioRxiv - Cancer Biology 2023

  Quote: ... pERK1/2 (1:1,000; Cell Signaling, #9101), ERK1/2 (1:1,000 ...
• Modulation of Viability, Proliferation, and Stemness by Rosmarinic Acid in Medulloblastoma Cells: Involvement of HDACs and EGFR

  Alice Laschuk Herlinger, et al., bioRxiv - Cancer Biology 2023

  Quote: ... AKT (1:1,000; Cell Signaling, #9272), p21 (1:1,000 ...
• Modulation of Viability, Proliferation, and Stemness by Rosmarinic Acid in Medulloblastoma Cells: Involvement of HDACs and EGFR

  Alice Laschuk Herlinger, et al., bioRxiv - Cancer Biology 2023

  Quote: ... pAKT (Thr308) (1:1,000; Cell Signaling, #13038), AKT (1:1,000 ...
• Modulation of Viability, Proliferation, and Stemness by Rosmarinic Acid in Medulloblastoma Cells: Involvement of HDACs and EGFR

  Alice Laschuk Herlinger, et al., bioRxiv - Cancer Biology 2023

  Quote: ... HDAC2 (1:1,000; Cell Signaling, #2540), histone H3K9ac (1:500 ...
• Modulation of Viability, Proliferation, and Stemness by Rosmarinic Acid in Medulloblastoma Cells: Involvement of HDACs and EGFR

  Alice Laschuk Herlinger, et al., bioRxiv - Cancer Biology 2023

  Quote: ... ERK1/2 (1:1,000; Cell Signaling, #4695), pAKT (Thr308 ...
• Modulation of Viability, Proliferation, and Stemness by Rosmarinic Acid in Medulloblastoma Cells: Involvement of HDACs and EGFR

  Alice Laschuk Herlinger, et al., bioRxiv - Cancer Biology 2023

  Quote: Cells were lysed after RA treatment for 48h with 1X lysis buffer (Cell Lysis Buffer 10 ×, Cell Signaling Technology, Danvers, USA), with protease and phosphatase inhibitors ...
• Transcriptional regulation of amino acid metabolism by KDM2B, in the context of ncPRC1.1 and in concert with MYC and ATF4

  Evangelia Chavdoula, et al., bioRxiv - Cancer Biology 2023

  Quote: Samples for ChIP-seq experiments were prepared using the SimpleChIP Plus Enzymatic chromatin IP kit (Cell Signaling Technology, Cat. 9005) according to the manufacturer’s protocol ...
• Differential modulation of miR-122 transcription by TGFβ1/BMP6: implications for nonresolving inflammation and hepatocarcinogenesis

  Martha Paluschinski, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and the Signal Silence Control siRNA (Cat.6568S; Cell Signaling, Danvers, MA, USA) were used ...
• Transcriptional regulation of amino acid metabolism by KDM2B, in the context of ncPRC1.1 and in concert with MYC and ATF4

  Evangelia Chavdoula, et al., bioRxiv - Cancer Biology 2023

  Quote: ... H3K27me3 1:50 (Cell Signaling Technology, Cat 9733), H2AK119ub 1:100 (Cell Signaling Technology ...
• Glioblastoma Mesenchymal Transition and Invasion are Dependent on a NF-κB/BRD2 Chromatin Complex

  Raghavendra Vadla, et al., bioRxiv - Cancer Biology 2023

  Quote: ... AKT (Cell Signaling Technology, #9272), anti-phospho-AKT Thr308 (Cell Signaling Technology ...
• NOP16 is a histone mimetic that regulates Histone H3K27 methylation and gene repression

  Ken Takashima, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-SP1(#9389, D4C3, Cell Signaling Technology, 1:1000), anti-HSP90 (#4874 ...
• NOP16 is a histone mimetic that regulates Histone H3K27 methylation and gene repression

  Ken Takashima, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-Cyclin E1(#20808, Cell Signaling Technology, 1:1000). For flow cytometry ...
• Systematic characterization of therapeutic vulnerabilities in Multiple Myeloma with Amp1q reveals increased sensitivity to the combination of MCL1 and PI3K inhibitors

  Romanos Sklavenitis-Pistofidis, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and Cas9 expression was confirmed by Western Blot (Cell Signaling Technology #14697). Subsequently ...
• AMPK targets PDZD8 to trigger carbon source shift to glutamine

  Mengqi Li, et al., bioRxiv - Cell Biology 2023

  Quote: ... Nonfat dry milk (cat. #9999) and normal goat serum (NGS; cat. #5425) were purchased from Cell Signaling Technology. ReverTra Ace qPCR RT master mix with a gDNA Remover kit was purchased from TOYOBO ...
• Selective inhibitors of the Aurora A-TPX2 protein-protein interaction exhibit in vivo efficacy as targeted anti-mitotic agents

  Simon R. Stockwell, et al., bioRxiv - Biochemistry 2023

  Quote: ... The cells were then sequentially stained in three steps with anti-Aurora A P-Thr288 (1:100, Cell Signaling #3079), goat anti-rabbit Alexafluor555 (1:500 ...
• Transgene-Free Ex Utero Derivation of A Human Post-Implantation Embryo Model Solely from Genetically Unmodified Naïve PSCs

  Bernardo Oldak, et al., bioRxiv - Developmental Biology 2023

  Quote: ... The antibodies and dilutions employed for cell immunofluorescence were the following: Rabbit polyclonal anti-Cdx2 (Cell Signaling Cat# 3977) 1:200 ...
• Polarized Desmosome and Hemidesmosome Shedding via Exosomes is an Early Indicator of Outer Blood-Retina Barrier Dysfunction

  Belinda J. Hernandez, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-Calreticulin (#12238 [clone D3E6]; Cell Signaling Technologies, Danvers, MA), HRP-conjugated donkey-anti-rat IgG (#712-035-153 ...
• ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulation

  Mohamed Abu-Farha, et al., bioRxiv - Biochemistry 2023

  Quote: ... Total NF-κB (8242s, Cell signaling), Phospho-IKKα/β (Ser176/180 ...
• ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulation

  Mohamed Abu-Farha, et al., bioRxiv - Biochemistry 2023

  Quote: ... Total IKKα (2682, Cell signaling), Phospho-IκBα (Ser32/36 ...
• Flow cytometric isolation of drug-like conformational antibodies specific for amyloid fibrils

  Alec A. Desai, et al., bioRxiv - Bioengineering 2023

  Quote: A human non-immune single-chain (scFv) library14 was sorted first against anti-Myc antibody (Cell Signaling, 2276S) to select for full-length scFv antibodies ...
• Identification of novel autophagy inducers by accelerating lysosomal clustering against Parkinson’s disease

  Yuki Date, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-ULK1 (Cell Signaling Technology Inc. AB_11178668), anti-phospho-p70S6K (Cell Signaling Technology Inc ...
• Conservation of the ancestral function of GTSF1 in transposon silencing in the unicellular eukaryote Paramecium tetraurelia

  Chundi Wang, et al., bioRxiv - Cell Biology 2023

  Quote: ... the membrane was incubated overnight at 4°C with a Rabbit anti-HA primary antibody (3724S, Cell Signaling Technology) at a dilution of 1:1000 ...
• S100A8/A9 predicts triple-negative breast cancer response to PIM kinase and PD-1/PD-L1 inhibition

  Lauren R. Begg, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PIM3 (clone D17C9, Cell Signaling Technology, 4165), human S100A8 (clone C-10 ...
• S100A8/A9 predicts triple-negative breast cancer response to PIM kinase and PD-1/PD-L1 inhibition

  Lauren R. Begg, et al., bioRxiv - Cancer Biology 2023

  Quote: ... STAT3 pS727 (Cell Signaling Technology, 9134), C/EBPβ (clone H-7 ...
• S100A8/A9 predicts triple-negative breast cancer response to PIM kinase and PD-1/PD-L1 inhibition

  Lauren R. Begg, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PPARG (clone C26H12, Cell Signaling Technology, 2435), Ubiquitin (clone P4D1 ...
• A mitochondria-regulated p53-CCF circuit integrates genome integrity with inflammation

  Karl N. Miller, et al., bioRxiv - Cell Biology 2023

  Quote: ... Phospho-p53 (Ser15) (Cell Signaling Technology Cat#9284S, RRID:AB_331464), Phospho-ATM (Ser1981 ...
• MET variants with activating N-lobe mutations identified in hereditary papillary renal cell carcinomas still require ligand stimulation

  Célia Guérin, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The folowing antibodies were used: MET (Cell Signaling #3148), MET-Phospho (Cell Signaling #3126) ...
• Aurora Kinase A Inhibition plus Tumor Treating Fields Suppress Glioma Cell Proliferation in a Cilium-Independent Manner

  Jia Tian, et al., bioRxiv - Cancer Biology 2023

  Quote: ... cells were harvested at indicated time points and lysed in 1x cell lysis buffer (Cell Signaling Technologies, Inc, Danvers, MA, USA; Cat#9803) or 1× radioimmunoprecipitation assay (RIPA ...
• A novel, RAS-independent role for NF1 in microtubular dynamics and damage repair dictates sensitivity to T-DM1 in HER2-positive breast cancer

  Bruno A. Duso, et al., bioRxiv - Cancer Biology 2023

  Quote: ... cells were incubated with primary antibody mouse anti-Tubulin Alexa Flour-488 conjugate (Cell Signaling Technologies, clone DM1A, #8058) diluted 1:100 in IF buffer for 5 hours ...