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Citations for Cell Signaling Technology :
901 - 950 of 10000+ citations for Recombinant Mouse Scavenger Receptor Class B Member 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... BSA was removed with PBS washes and primary antibodies detecting IdU and a protein of interest were diluted in 1% BSA/0.3% TritonX-100 and added to cells overnight at 4°C (IdU: mouse anti-BrdU, 1:20, Becton Dickinson; Total Histone H4: rabbit anti-Total Histone H4, 1:500, Upstate Cell Signaling Solutions 05-858 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were rinsed and incubated with Horseradish Peroxidase (HRP) conjugated anti-rabbit (1:5000) and anti-mouse (1:5000) secondary antibodies (Cell Signaling) for the rapamycin blot or with the fluorescent StarBright Blue 700 anti-mouse (1:5000 ...
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bioRxiv - Genetics 2022Quote: ... human anticentromere (1:100; Antibodies Inc., cat# 15-234) and mouse monoclonal anti-H3K9me2/3 (1:100, Cell Signaling, cat# 5327) primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... The dilutions of HRP-conjugated secondary antibodies were 1:2000 for goat-anti-guinea pig IgG and 1:2000 for anti-mouse IgG (Cell Signaling). Signal Fire ECL substrates (Cell Signaling ...
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bioRxiv - Neuroscience 2019Quote: ... Then the slices were incubated for 24 h at 4°C with primary antibodies, anti-mouse TH (dilution 1:1000, M318, millipore) and anti-rabbit c-Fos (dilution 1:200, 2250, Cell Signaling). The secondary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse lung cryosections were stained overnight with anti-BrdU (1:3, BD Biosciences, or 1:1000 Cell Signaling Technology, Danvers, MA) and incubated with Alexa Fluo 488-conjugated secondary antibody (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the following primary antibodies overnight: rabbit anti-RAD18 NB100-61063 (Novus) (1:200) and mouse anti-biotin (D5A7) (1:200) (Cell signaling). The rest of the protocol follows Proximity Ligation Assay as above.
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-Isl1/2 (Developmental Studies Hybridoma Bank, 1:100) and rabbit anti-β3-tubulin (Cell Signaling Technology, 5568S 1:200).
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bioRxiv - Microbiology 2020Quote: ... Nanobodies were incubated for 1 h at RT and detected using Mouse-anti-Myc antibody (1:1000, 9B11 clone, Cell Signaling). US28 was visualized with the rabbit-anti-US28 antibody (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.05% Tween 20] plus 5% BSA before incubation with the primary antibody (1:1000 for mouse monoclonal 12C11 anti-REST and 1:2000 for anti-LaminB1 rabbit polyclonal, Cell Signaling). Anti-rabbit IgG or anti-mouse IgG conjugated to horseradish peroxidase (1:2000 ...
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bioRxiv - Cell Biology 2019Quote: ... which was used at a 1:50 dilution in combination with a secondary anti-mouse HRP antibody (1:10,000, Cell Signaling #7076S). ECL Select (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated goat anti-mouse (1:4000) and goat anti-rabbit (1:4000) secondary antibodies were obtained from Cell Signaling Technology, Danvers ...
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bioRxiv - Cell Biology 2019Quote: ... anti-Mena (1:300, mouse monoclonal, a kind gift from Frank Gertler) and anti-Cre Recombinase (1:300, Cell Signaling 12830). Secondary antibodies used were goat anti-mouse or anti-rabbit IgG conjugated to Alexa Fluor-488 ...
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bioRxiv - Neuroscience 2023Quote: ... Respective expression was assessed by immunostaining using a mouse anti-HA antibody (1:500; Cell Signaling#2367, RRID AB_10691311 and a rabbit anti-Flag (1:500; Cell Signaling#14793 RRID AB_2572291 followed by Cy5-conjugated donkey anti-mouse (1.9 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2 ...
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bioRxiv - Cancer Biology 2023Quote: ... each membrane was probed with the appropriate horseradish peroxidase-conjugated secondary antibody (anti-mouse dilution 1:5,000; GTX213112-01, Gene Tex; anti-rabbit dilution 1:3000; 7074S, Cell Signaling Technology), and incubated for 1□h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were labeled with mouse anti-GFP (1:500) antibody and rabbit anti-FOXP1 antibody (Cell Signaling Technology; 1:400) and detected by biotinylated secondary antibody/streptavidin-Alexa Fluor 488 (GFP ...
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bioRxiv - Immunology 2023Quote: ... Tissues were then stained with the following fluorophore-conjugated anti-mouse antibodies for (1:100, 1.5 hr, room temperature, gentle agitation) in blocking buffer: Iba-1 (78060S, Cell Signaling Technology), F4/80 (123122 ...
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bioRxiv - Cell Biology 2024Quote: ... following incubation with the primary antibodies anti-p53 (1:500, mouse, (Ab-6) Pantropic OP43 Sigma-Aldrich) and anti-GAPDH (1:1000, rabbit, 14C10 Cell Signaling) for 1h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were incubated with conjugated antibodies LC3A/B-Alexa Fluor488 (Cell Signaling, #13082) and LAMP-1-Alexa Fluor546 (Santa Cruz ...
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bioRxiv - Neuroscience 2022Quote: ... LC3(A/B) (D3U4C) XP Rabbit monoclonal antibody was purchased from Cell Signaling (12741). All Alexa-fluorescent secondary antibodies were purchased from Fisher Scientific.
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 400 μL buffer B containing 2 μL Micrococcal Nuclease (#10011, Cell Signaling), and incubated for 20 min at 37°C with frequent mixing ...
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bioRxiv - Cell Biology 2023Quote: ... Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (Cell signaling technology, #2914), Aurora A (Cell signaling technology ...
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bioRxiv - Neuroscience 2023Quote: Staining for b-galactosidase was carried out using a commercial kit (Cell Signaling Technology) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The pull-down assay of ZFP982-MYC tagged protein was performed through co-Immunoprecipitation (co-IP) using MYC-Tag (9B11) antibody (#2276, Cell Signaling). For MYC-co-IP ...
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bioRxiv - Bioengineering 2019Quote: ... and stained following standard protocols previously described (Xue et al. 2011) with primary antibodies against 3xHA-tagged Nme2Cas9 (anti-HA; Cell Signaling) and mCherry (anti-RFP ...
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bioRxiv - Cancer Biology 2022Quote: Cells with stable expression of FLAG-tagged human PDE6D were lysed in 1X cell lysis buffer (#9803, Cell Signaling, Danvers, MA) and incubated with anti-FLAG M2 Affinity Gel (A2220 ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged and myc-tagged proteins were detected with rabbit anti-DYKDDDDK (FLAG) monoclonal antibody and rabbit anti-myc monoclonal antibody (Cell signaling), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... 1D4, DSHB), mouse anti-Fasciclin III (1:100; 7G10, DSHB) and rabbit anti-phospho-JNK/SAPK (1:100; 81E11 Cell Signaling Technology). Appropriate secondary antibodies conjugated either with Cy3 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... The primary antibody against phosphorylated RSKS-1 is anti-phospho-p70 S6 kinase (Thr389) (1A5) Mouse mAb (Cell Signaling, #9206, 1:1,000), which detects the conserved residue Thr404 in worms and was tested for its specificity using wildtype and rsks-1 deletion mutant lysates ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were washed with chilled FACS buffer (DPBS with 1% FBS and 2mM EDTA) and then incubated with the mouse anti-SSEA4 antibody (1: 50; 4755S, Cell Signaling Technology) on ice for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Neuroscience 2019Quote: ... 11A5(Anderson et al., 2006) (mouse monoclonal 11A5 kindly provided by Imago Pharmaceuticals, 1:25,000) and D1R1R (rabbit mAb #20706S, Cell signaling. 1:1000), NF-L (mouse mAb #2835 ...
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bioRxiv - Microbiology 2019Quote: ... 1:3000 diluted in 1% BSA) and mouse monoclonal anti- HA (6E2) tag antibody (1:1000 dilution in 3% bovine serum albumin, Cell signaling, #2367) for 1hr at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBS-T buffer and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit antibodies (1:5000; Cell Signaling Technology). The membranes were washed three times in TBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... as manufacturer’s recommended protocol following overnight incubation with anti-PAX8 mouse antibody 1:250 (Novus: NBP2-29903) and anti-SOX17 rabbit antibody 1:250 (Cell Signaling: 81778S) at 4 °C ...
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bioRxiv - Physiology 2021Quote: ... A mouse primary anti-CaSR monoclonal antibody (1:2000, Gentex, Zeeland, MI) or a mouse primary anti-Myc (9B11) monoclonal antibody (1:1000, Cell Signaling Technology) was applied followed by a secondary horseradish peroxidase-coupled secondary antibody (1:5000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the following antibodies were used anti-Bassoon (1:400, SAP7F407; mouse monoclonal; Enzo Life) and anti α-synuclein (1:500, D37A6; rabbit polyclonal; Cell Signaling). Species-specific secondary antibodies coupled to Alexa 488 and 350 (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... ERK1/2 (#9102, Rabbit, WB 1:1000) and MEK1/2 (#4694, Mouse, WB 1:1000) antibodies were purchased from Cell Signaling Technology, MA ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed in TTBS/1% skim milk, incubated with secondary antibodies (1:5000 anti-mouse IgG, HRP-linked antibody (Cell Signaling #7076S)) ...
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bioRxiv - Cell Biology 2023Quote: ... washed with TBST (3 ×10 min), incubated (1 hr, 20°C) in TBST containing HRP-conjugated secondary antibody (1:3000 anti-mouse, Cell Signaling Technology Cat# 7076S ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were combined with QD-fibril complexes in a total volume of 200 μL with 1% milk and 1:1000 mouse anti-Myc antibody (Cell Signaling, 2276S) and allowed to incubate with end-over-end mixing at room temperature for approximately 3 h ...
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bioRxiv - Immunology 2023Quote: ... The Ce3D™ Permeabilization/Blocking Buffer was discarded and replaced with Alexa Fluor 647 conjugated anti-mouse Iba-1 (1:100, 78060S, Cell Signaling Technology) in Ce3D™ Antibody Diluent Buffer (48 hr ...
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bioRxiv - Cell Biology 2023Quote: ... Cat. 97166 [D4C6R], mouse mAb, 1:1000; Akt, Cell Signaling, Cat. 4685 [11E7], rabbit mAb, 1:1000; p-Akt [Ser473], Cell Signaling, Cat ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibody solution contained goat anti-mouse antibodies FITCI at 1:1000 dilution and goat anti-rabbit dilution TRITCI at 1:1000 dilution (from Cell Signaling Technologies) and incubated in humidity chambers at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies used were as follows: HRP-linked anti-mouse IgG (Cell Signaling #7076, 1:5000); HRP-linked anti-rabbit IgG (Cell Signaling #7074 ...
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bioRxiv - Immunology 2021Quote: ... and anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2,000, Cell Signaling, Cat. no: 7076S).
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies used were as follows: mouse anti-SSEA4 (1: 200; 4755S, Cell Signaling Technology), rabbit anti-UCHL1 (1 ...
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bioRxiv - Molecular Biology 2021Quote: ... diluted 1:5000 or HRP mouse anti-rabbit IgG (conformation-specific) (Cell Signaling cat#L27A9; RRID:AB_10892860). All antibodies were diluted in 5% milk in TBS-Tween.
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bioRxiv - Molecular Biology 2021Quote: Cell lysates or mouse tissues were prepared using 1× cell lysis buffer (Cell Signaling Technology, USA) with 1 mM phenylmethylsulfonyl fluoride (PMSF ...