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Citations for Cell Signaling Technology :
901 - 950 of 1023 citations for Recombinant Human BSG Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Individual ChIP experiments were performed on pre-cleared chromatin using antibody-coupled ChIP grade Protein G magnetic beads (Cell Signaling Technology). POLR3A antibody was obtained from Abcam (ab96328 lot#GR318563) ...
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bioRxiv - Biochemistry 2021Quote: ... Primary antibody solution mix contains two primary antibodies raised in two different species (mouse and rabbit) targeting the proteins of interest: rabbit anti-mitofusin 2 (1:50, mAb D2D10, Cell Signaling), rabbit anti-VDAC (1:200 ...
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bioRxiv - Neuroscience 2021Quote: Antibodies for detection of cell surface proteins by western blot of cell lysates were used as provided and were from the following sources: anti-ErbB3 (Cell Signaling, HER3/ErbB3 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... MCF7 cell lysate as prepared above was diluted to 0.8 mg/mL total protein in 1:3 lysis buffer:dilution buffer with 0.5 nM rabbit anti-BRD4 antibody (Cell Signaling Technology; E2A7X) and 1 nM CoraFluor-1-labeled anti-rabbit nano secondary ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein levels were detected using antibodies against Intracellular Domain of Notch1 (ICN) (Val1744 Cell Signaling Technology #4147, D3B8, 1:500 dilution) and β-actin (Millipore-Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... The separated proteins on the gel were transferred onto PVDF membrane and were probed for specific antibodies against Rb (cat# 9309; Cell signaling) phospho-Rb (cat# 8516 ...
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bioRxiv - Cell Biology 2022Quote: ... protein blots were incubated with the following secondary antibodies Anti-Mouse IgG HRP Linked Antibody (Cell Signaling, Cat # 7076S, 1:2500) and Anti-Rabbit IgG HRP Linked Antibody (Cell Signaling ...
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bioRxiv - Genetics 2022Quote: ... Membranes were blocked in 4% BSA in 1X TBS and probed with S6 ribosomal protein 1:1000 (2217, Cell Signaling Technologies), phosphor-S6 ribosomal protein 1:1000 (2215 ...
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bioRxiv - Microbiology 2022Quote: ... The cells were harvested at 48 h pt and equal amounts of proteins were analyzed by western blot for p-eIF2α or total eIF2α (both antibodies were obtained from Cell Signaling). Expression of E was detected using an HA-tag antibody (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membranes at 100 volts for 90 minutes in cold 1X transfer buffer (Cell Signaling Technology, 12539S). Membranes were blocked with 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... ADP-ribosylated proteins were visualised by chemiluminescence using the SignalFire ECL Reagent or the SignalFire Elite ECL Reagent (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Samples used for measuring HMGB1 protein levels in the stool and colonic mucus were homogenized in cell lysis buffer (Cell Signaling) containing complete protease inhibitor (Roche ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Primary antibodies/concentrations used were as follows: rabbit anti-phospho-S6 ribosomal protein (Serine240/244) (1:100, Cell Signaling Technologies, #2215), rabbit anti-phospho-S6 ribosomal protein (Serine244/247 ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to nitrocellulose membrane (Cytiva) and revealed using following antibodies: rabbit AGR2 (Cell Signaling Technologies, Clone D9V2F, 1/1000), mouse FLAG-M2-HRP (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Protein accumulation in the purified fractions was confirmed with western blot using anti-MBP tag (8G1) mouse monoclonal antibody (Cell Signaling) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Primary antibodies against following proteins were used: MYPT1 (rabbit IgG mAb clone D6C1, #8574) from Cell Signaling Technology (Leiden, The Netherlands), MYPT1 (mouse IgG1 mAb clone C-6 ...
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bioRxiv - Neuroscience 2024Quote: ... every 8th section of the brain was processed for double-fluorescent immunostaining for Ku80 and one of the following: Microtubule-associated protein 2 (MAP2; 8707, Cell Signaling), doublecortin (ab18723 ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were collected and incubated with a 30 μl bed volume of protein-A-sepharose beads and 1.5 μl of α-IGF-1 receptor β (D23H3) XP (Cell Signaling) per sample ...
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bioRxiv - Molecular Biology 2019Quote: 500 μg of unstimulated HT29 lysate was immunoprecipitated onto Protein A/G coated wells (Pierce #15138) following the kinase assay protocol but with 0.5 μg of IKKα (Cell Signaling Technology #2682) or 0.5 μg of naïve rabbit IgG (Jackson Immunoresearch #011-000-002) ...
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bioRxiv - Immunology 2021Quote: ... Western blotting was used as the output assay to determine levels of the ribosomal protein S6 kinase (p-S6K) using an S6K and p-S6K antibodies (Cell Signaling Technology). To assess the impact of mTOR1 inhibition rapamycin (20ng/mL ...
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bioRxiv - Microbiology 2020Quote: ... 5 mg of tissue from distal ileal mucosal scrapings was lysed in 250 μL of ice-cold protein lysis buffer (Cell Signaling Technology Cell Lysis Buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein level could also be measured by immunofluorescence using fluorescently labeled antibodies (anti-Flag-Alexa 647, #15009; Cell Signaling, Danvers, MA, USA). These antibodies were flowed into the device and incubated with the immobilized proteins under the ‘button’ for 20 min at RT ...
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bioRxiv - Plant Biology 2021Quote: ... The phosphorylated MAPK3 and MAPK6 proteins were detected using polyclonal anti-phospho-p44/42 MAPK (Erk1/2, 1:1000; Cell Signaling Technology), and the detection of the total MAPK3 and 6 protein levels were by using polyclonal anti-MAPK3 (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Ten to 50 μg of proteins were separated by 10 % SDS-PAGE and visualized with primary Slug or E-cadherin antibody (C19G7, Cell Signaling Technology, #9585 ...
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bioRxiv - Biochemistry 2019Quote: ... in tris-buffered saline Tween 20 (TBS-T) for 1 h at RT and then blotted for phosphorylated proteins (Cell Signaling Technology; phospho-EGFR Y1068 #3777 ...
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bioRxiv - Neuroscience 2020Quote: ... 20 µg of total brain protein were loaded and anti-GFAP antibody (D1F4Q) XP Rabbit mAb (1:3000; Cell Signaling Technology, 12389), anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... The expression of PSMA protein in each cell line was investigated by western blot using anti-PSMA rabbit antibody (Cell Signaling Technology) [11].
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bioRxiv - Biochemistry 2021Quote: ... Proteins were immunoprecipitated from precleared lysates overnight at 4°C using 4 μg of control IgG (rabbit or mouse, Cell Signaling Technology) or indicated antibody ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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bioRxiv - Immunology 2020Quote: ... To detect phosphorylated ERM proteins a rabbit anti-phospho–ezrin (Thr567)/radixin (Thr564)/moesin (Thr558) (pERM) antibody was used (Cell Signaling Technology). Isotype control staining was performed using rabbit IgG isotype mAb Alexa Fluor 647 (DA1E ...
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bioRxiv - Biophysics 2022Quote: ... 15 μg of total protein was loaded onto an SDS-PAGE (4-12%) to assess LRP6 and β-catenin protein levels using phospho- and total LRP6 (Cell Signaling), active- and total β-catenin (Cell Signaling ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h at room temperature and blotted with an antibody against phosphorylated upstream frameshift protein 1 (phospho-UPF1) (pUPF1; 1:1000, Cell Signaling Technologies) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Specimens were incubated overnight at 4 °C with rabbit anti-SARS-CoV-2 nucleocapsid protein (Cell Signaling Technology, USA, #26369, 1:200), mouse anti-ACE2 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... cFos protein was labelled in the same manner using rabbit polyclonal antibody c-Fos (9F6) (1: 1000, Cell Signaling Technology Inc, 2250) and Alexa Fluor 488 AffiniPure F(ab’)□ Fragment Donkey Anti-Rabbit (1:200 ...
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bioRxiv - Developmental Biology 2024Quote: MCF10A cells from 2D collagen stimulation assays were pelleted by centrifugation and lysed using 1X RIPA Buffer containing both 1x protease inhibitor cocktail and 1x phosphatase inhibitor Lysate was precleared with Protein-A Magnetic Beads (73778, Cell Signaling Technology).
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bioRxiv - Microbiology 2024Quote: ... the ADP-ribosylated proteins were visualized using chemiluminescence using the SignalFire ECL Reagent or the SignalFire Elite ECL Reagent (Cell Signaling Technology) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... and then the supernatant fraction was incubated with anti-BCR-ABL antibody overnight at 4°C and subsequently with protein A magnetic beads (73778, Cell Signaling Technology) for another 4 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... For visualization of SARS-CoV-2 infected cells via detection of N protein in Calu-3 cells we used anti SARS-CoV-1/2 Nucleocapsid Mouse anti-N clone (Cell Signaling, 1C7C7) 1:1000 50uL/well.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then prepared for Western blot analysis and total protein was incubated with E-Cadherin (1:1000, Cell Signaling Technology, #3195), Fibronectin (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The phosphorylated proteins were detected by probing the membranes with an anti-phospho-c-Met antibody (Tyr1234/1235, #3077, Cell Signaling Technology) or an anti-phospho-EGFR antibody (Tyr1068 ...
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bioRxiv - Biophysics 2023Quote: ... blocked with 5% bovine serum albumin for 1 hour at room temperature and incubated overnight with a primary antibody against the principal ventricular GJ protein Cx43 phosphorylated at Ser368 (pCx43, 1:1000, #3511S, Cell Signaling Technologies), at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Western blot analysis was performed to assess total and phosphorylated p70S6K1 (70kDa ribosomal protein S6 kinase) (T389, Cell Signaling Technology; Boston, MA) as well as 4EBP1 (4E-binding protein 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... HER2 protein detection on EVs was performed by staining samples with HER2/ErbB2 (29D8) – PE-conjugated antibody or Isotype Control (Cell Signaling Technology) and CellMask plasma membrane stains (C10046 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein lysates were separated by SDS-PAGE and proteins of interests detected with the following antibodies: phosphorylated (p-VASP) Ser239 (rabbit, 1:1000, Cell Signaling #3114S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Every gel included two molecular-weight markers for separate regions of interest (High Range Color-Coded Prestained Protein Marker; Cell Signaling Technology; and Novex Prestained Protein Standard ...
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bioRxiv - Neuroscience 2023Quote: ... anti-Phospho-p70 ribosomal protein S6 kinase (Thr389) (108D2) (pS6K) (rabbit monoclonal, #9234, RRID: AB_2269803, Cell Signaling Technology; 1:1000 for WB), anti-p70 S6K α (S6K)(mouse monoclonal clone H-9 ...
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bioRxiv - Immunology 2023Quote: ... cycloheximide (CHX) final concentration of 8 ug/mL was used to arrest protein translation and Actinomycin D (Cell Signaling Technology, Cat. 15021) was used to arrest transcription by treating cells with 10 ug/mL Actinomycin D where indicated ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for more than 3 hours with a protein complex of anti-HA antibody (Cell signaling technology) and Dynabeads Protein A (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Protein expression levels were detected with the following antibodies: Jak-1 (6G4) and phospho-Jak-1 (Tyr1034/1035) (from Cell Signaling Technology), IL-10Rα (A3 ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained at 1:100 with anti-phospho S6 ribosomal protein (Ser235/236) Alexa Fluor 647 (D57.2.2E, Cell Signaling Technologies cat#4851S).