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Citations for Cell Signaling Technology :
901 - 950 of 1117 citations for 7 benzyl 2 7 diazaspiro 4.5 decane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... followed by addition of secondary Alexa Fluor 488 anti-mouse IgG Fab 2 antibody (Cell Signaling Technology 4408S) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used : Phospho-myosin light chain 2 (Ser19) (1:200; Cell Signaling Technology #3671), Non-muscle myosin IIA (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... or (2) with a cocktail of rabbit anti c-Fos (1:500, Cell Signaling Technology, Cat. No. 2250) and mouse anti PV (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... MAPK phosphorylation was determined using an anti-phospho-p44/42 MAPK (Erk1/2) antibody (anti-pMAPK; Cell Signaling).
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with an anti-SARS-CoV-1/2 nucleocapsid primary antibody (Cell Signaling Technology; 68344) followed an Alexa Flour 555 fluorescent anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by staining with an AlexaFluor647-conjugated anti-rabbit IgG F(ab’)2 Fragment (1:800, Cell Signaling). 0.5% BSA in 1X PBS was used as a staining and washing buffer ...
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bioRxiv - Cell Biology 2022Quote: ... F(ab’)2 Fragment (Alexa Fluor® 555 Conjugate) (catalog 4413) antibodies were obtained from Cell Signaling Technology; α-actin (catalog 53141 ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit polyclonal against Phospho-Myosin Regulatory Light Chain 2 at position Ser19 (Cell Signaling, Danvers, MA; catalog # 3671) was used at 1/100 ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (1:500, 4370, Cell Signaling Technology, MA, USA), mouse anti-beta Catenin (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... Lysed cells were pelleted and precleared with 2 μg/ml anti-rabbit isotype IgG antibody (Cell Signaling #2729) with protein A/G magnetic beads (Pierce ...
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bioRxiv - Microbiology 2024Quote: ... then nutated in 75 µl PBS BSA with 2 µl Cas9 antibody (Cell Signaling Technology Cas9 (7A9-3A3) Mouse mAb #14697 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with a primary antibody for Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling) for 48 hours at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... or Phospho-p44/42 MAPK (erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit monoclonal antibody (Cell Signaling Technology; 4370). Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat (A0545 ...
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bioRxiv - Cell Biology 2019Quote: ... anti-phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204) rabbit antibody (no. 9101, Cell Signaling Technology, 1:2,000 dilution); anti-α-Actin rabbit antibody (no ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... Western Blots were performed with the following primary antibodies: rabbit-anti-p-ERK1/2 (1:500, Cell Signaling 9101), rabbit-anti-Erk1/2 (1:500 ...
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bioRxiv - Cancer Biology 2019Quote: ... samples were blocked with 2% BSA and incubated with primary antibodies including Rabbit anti-ALK (1:200, Cell Signaling), Rabbit anti-MYCN (1:50 ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μg of nuclear protein lysates were incubated with 2 μg of either Myc-Tag antibody (Cell Signaling Technology) or an endogenous NUP210-specific antibody (Bethyl Laboratories) ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used for western blotting: Rabbit anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19) (3674, Cell Signaling Technology), rabbit anti-Myosin Light Chain 2 (3672 ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody used for immunofluorescence include anti-phospho-p44/p42 ERK1/2 (Thr202/Tyr204) (Cat# 4370) from Cell signaling technologies used at 1:200 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (clone D13.14.4E) (#4370; 1:1000) were purchased from Cell signaling. M2 anti Flag Mouse antibody (#SLBT7654 ...
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bioRxiv - Cell Biology 2022Quote: ... and the supernatant containing fragmented chromatin was incubated with 2 μl of anti-YY1 antibody (D5D92Z, Cell Signaling, 46395) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... 10 μM of p38 inhibitor (SB203580) or MEK1/2 inhibitor (U0126) was used (Cell Signaling Technology, Danvers, MA, USA). Cells were monitored by live cell imaging using the BD Pathway 855 Bioimaging System (BD Biosciences) ...
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bioRxiv - Plant Biology 2019Quote: ... or Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit monoclonal antibody (4370; Cell Signaling Technology). Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat (A0545 ...
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bioRxiv - Cell Biology 2019Quote: The following small molecule inhibitors were used in this study: MEK inhibitors: 2 μM PD 184352 (Cell Signaling 12147) and 10 μM Selumetinib (Selleckchem S1008) ...
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bioRxiv - Cell Biology 2019Quote: ... 555 or 647 Goat anti-Mouse or Goat anti-Rabbit IgG secondary antibodies (2 μg/mL, Cell Signaling Technology), Alexa Fluor 488 or 555 Phalloidin (33 mM ...
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bioRxiv - Developmental Biology 2021Quote: ... Ward) at 1:400 concentration or a rabbit anti-phospho-Myosin Light Chain 2 (Ser19) (#3671, Cell Signaling Technology) at 1:50 concentration ...
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bioRxiv - Developmental Biology 2020Quote: Cell lysates were prepared in 1x SDS Sample Buffer (62.5 mM Tris-HCl pH 6.8, 2%SDS, 42mM DTT, 10% glycerol and 0.01% bromophenol blue; Cell Signaling Technology), sonicated to shear DNA and reduce viscosity and then heat denatured ...
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bioRxiv - Developmental Biology 2022Quote: ... the following primary antibodies were used: Rabbit monoclonal antibody (mAb) to Snail-2 (clone C19G7, Cell Signaling, 1/300), mouse mAb to Sox-10 (Clone A2 ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining was carried out by using 4’,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA). Four different sections with no tissue loss were counted for the quantification of cells ...
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bioRxiv - Cancer Biology 2022Quote: ... antibodies (RRID:AB_330744) and monoclonal mouse anti-phospho-p44/42 MAPK antibodies (pERK1/2; pT202/pY204) (RRID:AB_331768) were from Cell Signaling.
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bioRxiv - Developmental Biology 2023Quote: ... Nuclear staining was carried out by using 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA). Larvae were imaged using fluorescence confocal microscopy.
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg of protein were added to pre-washed 50 μL streptavidin Sepharose bead conjugates (Cell Signaling Technology, 3419S) and rocked overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (4370, 1:1000, Cell Signaling Technology), anti-CD9 (ab307085 ...
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bioRxiv - Bioengineering 2023Quote: ... anti-pSmad 1/5/9 (ref: 13820S) and anti-pSmad 2 (ref: 18338S) antibodies were obtained from Cell signaling. The secondary antibody conjugated to Alexa555 or Alexa488 were purchased from Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was incubated overnight at 4 °C with primary antibodies against phospho-ERK1/2 (1:1,000; Cell Signaling), ERK1/2 (1:400 ...
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bioRxiv - Immunology 2024Quote: ... The fixed cells were incubated for 2 hrs at room temperature with β-actin antibody (Cell Signaling Technology, 4967) at 1:1000 dilution and then incubated with VRDye 549 Goat anti-Rabbit IgG Secondary Antibody (LI-COR biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... blocked in 2% BSA for 1h and stained with primary anti-UBA1a/b antibodies (Cell Signaling, 4891S, 1:500) for 1h ...
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bioRxiv - Molecular Biology 2024Quote: ... and the anti-phospho-Thr202/Tyr204-ERK1/2 polyclonal antibody were purchased from Cell Signaling Technologies (Beverly, MA, USA). The anti-cyclin B1 monoclonal antibody was from BD Biosciences Pharmingen ...
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bioRxiv - Cancer Biology 2021Quote: ... permeabilised with 90% (v/v) methanol and then intracellularly stained with anti-phospho-p44/42 MAPK (Erk1/2; Cell Signaling) for 30 min at room temperature and then with secondary antibodies against rabbit IgG (H+L ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: The kinase inhibitors used were against cSrc (Dasatinib 100nM), AKT (Perifosine 10μM), ERK1/2 (U0126, 10μM) (0952, 14240 and 9903, respectively, Cell Signaling Technologies) and pNaKtide (1μM ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated overnight at 4°C with the rabbit anti-phospho-p44/42 MAPK (for pErk1/2) (Cell Signaling Technology) at 1:250 ...
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bioRxiv - Cancer Biology 2019Quote: ... Other primary antibodies used are anti–Phospho-p44/42 MAPK (phosphorylated ERK1/2, Thr202/Tyr204, 197G2, Cell Signaling Technology #4377), anti-p44/42 MAPK (ERK1/2 ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated overnight with rabbit polyclonal primary antibodies against phospho-p44/42 MAPK (Erk1/2) (Cell Signaling, 1:2000) at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The following antibodies were used: rabbit anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370, 1:100, Cell Signaling), rabbit anti-Phospho-Drosophila p70 S6 Kinase (Thr398 ...
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bioRxiv - Physiology 2022Quote: ... anti-p44/42 MAPK (Erk1/2) (#4695) and anti-Acetyl-α-Tubulin (Lys40) (#5335) antibodies were from Cell Signaling Technology. Anti-Lamin A/C (sc-20681) ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibodies used for immunofluorescence staining were as follows: anti-Phospho Myosin Light Chain 2 (Cell Signaling Technology, #3671), anti-ZO-1 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... and the plate was extensively washed with TBST + 100 mM EDTA and a 1:8,000 dilution of primary antibody (p-MEK1/2 (S217/S221) Rabbit Antibody (Cell Signaling)) was added to the plate and incubated for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies against the following proteins were used in the indicated dilutions: ERK1/2 (#4695, RRID:AB_390779, Cell Signaling Technology, 1:50), JUN (#9165 ...
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bioRxiv - Cancer Biology 2020Quote: ... phospho-Erk1/2 T202/Y404 (4370) 1:2000 and Erk1/1 (4695) 1:2000-1:5000 (all from Cell Signaling); and Tubulin (T5168 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of fusion proteins and 8 μg of FLAG-PKD1-CA were incubated in kinase buffer (Cell Signaling Technology) for 30 min at 30°C in the presence of 200 μM ATP ...