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Citations for Cell Signaling Technology :
901 - 950 of 1036 citations for 3 Thiophen 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 × 10 min with TBST and incubated for 1 h with the appropriate secondary antibody (Cell Signaling Technologies) diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were incubated with a polyclonal cleaved caspase 3 primary antibody (rabbit, 1:100; 9661, Cell Signaling, Frankfurt am Main, Germany) for 60 min at room temperature followed by incubation with a monoclonal secondary horseradish peroxidase (HRP)-coupled horse-anti-rabbit antibody with AEC+ as chromogen ...
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bioRxiv - Cell Biology 2021Quote: ... Protein accumulation rate upon proteasome inhibition was measured after 3-hour treatment with 10μM of MG-132 (Cell Signaling Technology #2194) in the RPMI medium.
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bioRxiv - Neuroscience 2020Quote: ... They were then incubated overnight at 4°C with the following primary antibodies diluted in PBS - 0.3% Triton X100 - 1% normal serum: rabbit anti-cleaved caspase 3 (1/400, Cell Signaling, 9661), rabbit anti-doublecortin (1/2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse lung cryosections were stained overnight with anti-BrdU (1:3, BD Biosciences, or 1:1000 Cell Signaling Technology, Danvers, MA) and incubated with Alexa Fluo 488-conjugated secondary antibody (1:200 ...
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bioRxiv - Cell Biology 2021Quote: Human paraffin embedded pancreas biopsies were sectioned to 3 μm and immunostained with antibodies against MAFB (Cell Signaling Technologies, cat#41019), ARX (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 h of paraquat treatment cells were fixed with 4% PFA and stained for cleaved caspase 3 (Cell Signaling, 9661).
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bioRxiv - Cancer Biology 2022Quote: Human cancer tissue array staining was subjected to antigen retrieval at 98 °C for 20 min in EDTA buffer (pH 9.0) in a steamer and incubated with a rabbit anti-cleaved caspase 3 antibody (Cell Signaling, 9664L) 1:150 overnight at 4 °C followed by anti-rabbit 594 (Invitrogen A11012 ...
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bioRxiv - Microbiology 2020Quote: ... Lysed samples were centrifuged and incubated for 3 h with Pierce Protein A/G Magnetic beads (88802) which were pre-incubated over night with V5 antibody (Cell signaling E9H80 ...
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bioRxiv - Physiology 2020Quote: ... 1:100; rabbit anti-vinculin, 1:100, ab129002, Abcam, Cambridge, UK; rabbit anti-cleaved caspase-3, 1:400, 9446, Cell Signaling) were applied overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: Immunohistochemistry (IHC) was conducted on formalin fixed paraffin embedded tissues using cleaved caspase 3 antibody (1:300, Cell Signaling, Cat# 9661), EnVision+ Dual Link HRP secondary (Agilent ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... MCF7 cell lysate as prepared above was diluted to 0.8 mg/mL total protein in 1:3 lysis buffer:dilution buffer with 0.5 nM rabbit anti-BRD4 antibody (Cell Signaling Technology; E2A7X) and 1 nM CoraFluor-1-labeled anti-rabbit nano secondary ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were blocked with 3% BSA and incubated with primary antibodies against pERK1/2 (1:500, Cell Signaling Technology, 4370) or cleaved Caspase-3 (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... and 8 μg of total protein subjected to immunoblotting against cleaved caspase-3 and −7 (1:200 dilution; RRID: AB_2341188 and RRID: AB_11178377; cat. no. 9661S and 8438S, Cell Signaling, Danvers, MA) as well as phosphorylated (1:500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was washed 3 times with TBS-T and incubated with horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology #7074) for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... cleaved caspase 3 (9664, Cell Signalling, 0.5 μg ml-1) and cleaved-caspase 9 (9509, Cell Signaling, 1 μg ml-1). Secondary antibodies included Alexa Fluor 488 donkey anti-goat (A11055 ...
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bioRxiv - Neuroscience 2023Quote: 25 mg cortical tissues were dissected from wild type mice (n = 3) and chromatin was prepared using the SimpleChIP plus Enzymatic Chromatin IP Kit (Cell Signaling). UBE3A-bound chromatin was immunoprecipitated using rabbit anti-UBE3A antibody (Bethyl Laboratories) ...
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bioRxiv - Cancer Biology 2022Quote: ... #9188),Ki-67 (1:1,000 dilution; Novocastra; NCL-KI-67-P) and Cleaved Caspase 3 (1:200 dilution; Cell Signaling, #9661). p-CREB 1:100 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... immunoblots were probed for anti-rabbit acetylated histone 3 at lysine 9 and histone 4 at lysine 5 (ac-H3K9 and ac-H4K5 respectively, Cell Signaling), anti-rabbit LC3B (Abcam) ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h and incubated overnight at 4°C with the corresponding primary antibody diluted in blocking solution: Anti-Caspase 3 (rabbit, 1:100, Cell Signaling). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used for immunohistochemical studies included: anti-IGF-1R (#14534) and anti-cleaved-caspase 3 (#9661) from Cell Signaling Technology; and anti-CD99 (#ab-227738 ...
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bioRxiv - Microbiology 2023Quote: ... 3% BSA overnight at 4 □C (rabbit α-LC3B 1:1000 – Cell Signaling/ #4108, rabbit α-Atg5 1:1000 – Cell Signaling/#2630 ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used in this study were 1:2,500-diluted mouse anti-β-actin (Wako, #017-24551) and 1:1,000-diluted rabbit anti-cleaved caspase-3 (Cell Signaling, #9661). The membrane was washed three times with PBS for 10 minutes and incubated with secondary antibodies at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... The fixed and permeabilized cells were then labelled with antibodies against cleaved caspase 3 (Cell Signaling Technology; 9761; 1:500 dilution) and counterstained with DAPI ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were washed 3 times with 1x TBST,and incubated with either anti-rabbit IgG HRP-linked antibody (Cell signaling, 7074) or anti-mouse IgG HRP-linked antibody (Cell signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed for 10 minutes with PBS-T 3 times and then incubated in DyLight secondary antibodies (Cell Signaling Technologies) for one hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was then washed 3 times in 1x TBST and 1:3000 anti-rabbit secondary antibody (Cell Signaling Technology #7074) in 5% milk TBST was added ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by 3 5-minute washes by 1x TBST and probing with horseradish peroxidase conjugated secondary antibodies (Cell Signaling, 7074, 7076) for 1-1.5 hours at 25°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Single clones were picked out after 3 weeks and Cas9 expression level in each clone was determined by western blotting with Cas9 antibody (Cell Signaling, #14697). Cas9 activity was tested using the CRISPRtest™ Functional Cas9 Activity Kit for Human Cells (Cellecta ...
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bioRxiv - Cell Biology 2020Quote: ... Cleaved caspase-3 was probed in MuSCs by incubating the cells overnight in 1:100 Cleaved Caspase-3 (Asp175) (5A1E) rabbit mAb (Cell Signaling Technology). MuSCs were stained for 3 hours with 1:500 donkey anti-rabbit Alexa 594 secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... IL-1β and KC in the cell lysates and the culture supernatants were determined by western blot analysis using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as loading control with specific antibodies (Cell Signaling Technology) as previously described (19).
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bioRxiv - Cancer Biology 2020Quote: ... Slides were washed 3 times with PBS and incubated in secondary antibody (anti-mouse [Cell Signaling, #4408S]; anti-rabbit [Cell Signaling, #8889S]) for 1.5 hours ...
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bioRxiv - Cell Biology 2021Quote: ... and Cleaved Caspase 3 (#9664, 1:400 dilution for IF) and anti-Rsk2 (#5528; 1:200 dilution for IF) were purchased from Cell Signaling Technology. Anti-Tubulin α (66031-1-Ig ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 3% BSA or 5% milk and probed with the following primary antibodies-1:1000 rabbit anti-Flag (Cell Signaling technology); 1:100 chicken anti-E.histolytica CSN5 (custom) ...
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bioRxiv - Microbiology 2020Quote: ... Flare Biotech LLC) following by HRP conjugated goat anti-rabbit antibody (1:10,000 Ab in 3 % BSA in TBST; Cell Signaling Technology Inc). Images obtained were analyzed via ImageJ software.
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bioRxiv - Cancer Biology 2022Quote: ... and ɣH2AX (NB100-384) were from Novus; cleaved Caspase-3 (5A1E; 9664) and pS10 Histone H3 (9701) were from Cell Signaling Technology; GAPDH (6C5 ...
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bioRxiv - Physiology 2022Quote: ... antibody in 0.1% TBST containing 3% BSA overnight at +4°C followed by incubation with secondary HRP-linked anti-rabbit antibody (Cell Signaling, #7074). Immunoblotted proteins were detected with ProSignal Pico ECL Reagent (Genesee Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... sections were blocked with 3% bovine serum albumin / TBS and then incubated with primary antibodies (rabbit anti α-actinin (Cell Signaling, 3134)) overnight at 4 °C at 1:30 ...
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bioRxiv - Bioengineering 2022Quote: ... were washed with TBS buffer 3 times for 5 minutes and subjected to antigen retrieval using 1X SignalStain® Citrate Unmasking Solution (Cell signaling). The citrate buffer was bought to boiling temperature and added to the sections ...
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bioRxiv - Bioengineering 2021Quote: ... The fixed samples were permeabilized in PBS containing 0.1% Triton X-100 and 1% Fetal Bovine Serum for 30 min at room temperature before filling the channels with staining buffer (1.5% BSA in PBS) containing primary antibodies directed against cleaved caspase-3 (Cell Signaling Technology, 9661S) or VE-cadherin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Galectin-3 (#87985, IF: 1:400) and Na/K-ATPase α1 (#23565, IF: 1:100) antibodies were purchased from Cell Signaling Technology. Anti-beta-actin antibody (A2228 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then stained overnight at 4°C with AlexaFluor 647-conjugated anti-β-catenin antibody L54E2 at 1:300 dilution supplemented with 3% BSA (2677S, Cell Signaling). Between and after each of these steps ...
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bioRxiv - Bioengineering 2021Quote: ... brains were formalin fixed and paraffin embedded before staining with cleaved caspase-3 (CC3 Rabbit Mab, 1:800 #9664L [D175] Cell Signaling Technology) and rabbit polymer secondary (Biocare Medical # RMR 622L) ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies (diluted in 3% w/v BSA in TBST) were used: adipose triglyceride lipase (ATGL) (1:1000, Cell Signaling #2138), perilipin5 (PLIN5 ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL of aprotinin and leupeptin] and incubated (16 hrs., 4°C) with either 3 µg non-immune control rabbit IgG (Cell Signaling #272) or with 3 µg of ERRα antibody (Cell Signaling #13826 ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in antibody solution containing 2% normal donkey serum and 0.3% Triton X-100 in PBS with primary rabbit anti-activated caspase 3 antibody (Cell Signaling, Cat# 9661). Sections were washed five times for five minutes in PBS ...
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bioRxiv - Immunology 2022Quote: ... Membranes were washed 3 times with TBST and then incubated with peroxidase-conjugated secondary antibody (Cell Signaling Technology, Cat.No. 7074S, 1:3000). The antigen-antibody reaction was visualized by enhanced chemiluminescence assay (ECL ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized with 0.3% Triton X-100 in PBS (PBST) prior to blocking and staining with rabbit anti-acetylated alpha tubulin antibody (Cell Signaling #5335). Confocal 3D images of whole islets were used for cilia frequency and length quantitation ...
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bioRxiv - Cell Biology 2022Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Physiology 2022Quote: ... The following day sections were washed in wash buffer 3 times for 5 min and co-stained for anti-Rabbit IgG Fab2 Alexa Fluor 488 (Cell Signaling, 4412S) at 1:1000 dilution at room temperature for 1 h ...