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• Focal adhesions are controlled by microtubules through local contractility regulation

  J. Aureille, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197)4) (Cell Signaling, cat. no. #2605, dilution 1:2000); anti-acetyl-α-Tubulin (Lys40 ...
• Focal adhesions are controlled by microtubules through local contractility regulation

  J. Aureille, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-acetyl-α-Tubulin (Lys40) (Cell Signaling, cat. no. #3971, 1:1000); anti-αTAT1 (Thermo Fisher Scientific ...
• Focal adhesions are controlled by microtubules through local contractility regulation

  J. Aureille, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-ARP2 (Cell Signaling, cat. no. #3128, dilution 1:1000); anti-Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197)4 ...
• Comparison of commercially available differentiation media on morphology, function, and virus-host interaction in conditionally reprogrammed human bronchial epithelial cells

  Nikhil T Awatade, et al., bioRxiv - Cell Biology 2023

  Quote: ... Membranes were washed in PBS and incubated with fluorescent secondary antibody Goat anti-mouse Alexafluor 594 (8890, Cell signaling technology, USA). Membranes were mounted on slides with Fluoromount-G containing DAPI (00-4959-52 ...
• Differential roles of CTP synthetases CTPS1 and CTPS2 in cell proliferation

  Norbert Minet, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-GFP (Cell Signaling 2956S). The following antibodies were used for detection ...
• ATP13A4 upregulation drives the elevated polyamine transport system in the breast cancer cell line MCF7

  Sarah van Veen, et al., bioRxiv - Cell Biology 2023

  Quote: ... The following antibodies were purchased from Cell Signaling Technology: phospho-JNK antibody (9251) ...
• The adhesion GPCRs CELSR1-3 and LPHN3 engage G proteins via distinct activation mechanisms

  Duy Lan Huong Bui, et al., bioRxiv - Cell Biology 2023

  Quote: ... and subsequently incubated with diluted primary HA tag antibody (anti-HA rabbit (Cell Signaling Technologies Cat# 3724; 1:2,000)) in blocking buffer for 2 hours at room temperature ...
• Xrp1 governs the stress response program to spliceosome dysfunction

  Dimitrije Stanković, Luke Tain, Mirka Uhlirova, bioRxiv - Cell Biology 2023

  Quote: ... anti-phospho-histone H3 (rabbit, 1:500; Cell Signaling Technology #9701; RRID:AB_331535), anti-Dcp-1 (rabbit ...
• Xrp1 governs the stress response program to spliceosome dysfunction

  Dimitrije Stanković, Luke Tain, Mirka Uhlirova, bioRxiv - Cell Biology 2023

  Quote: ... anti-Dcp-1 (rabbit, 1:500; Cell Signaling Technology #9578; RRID:AB_2721060), anti-Mmp1 antibody mix (mouse ...
• Adapting the endoplasmic reticulum proteostasis rescues epilepsy-associated NMDA receptor variants

  Pei-Pei Zhang, et al., bioRxiv - Cell Biology 2023

  Quote: ... The mouse polyclonal anti-CHOP antibody was obtained from Cell Signaling (catalog #: 2895).
• Anti-nucleolin aptamer, iSN04, inhibits the inflammatory responses in myoblasts by modulating the β-catenin/NF-κB signaling pathway

  Machi Yamamoto, et al., bioRxiv - Cell Biology 2023

  Quote: ... or rabbit polyclonal anti-β-catenin antibody (#9562; Cell Signaling Technology, Danvers, MA, USA). Alexa Fluor 488-conjugated donkey polyclonal anti-rabbit IgG antibody (Jackson ImmunoResearch ...
• The adhesion GPCRs CELSR1-3 and LPHN3 engage G proteins via distinct activation mechanisms

  Duy Lan Huong Bui, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-HA rabbit (Cell Signaling Technologies Cat# 3724; 1:2,000), Alexa Fluor 647 Phalloidin (Invitrogen Cat# A22287 ...
• Retroelement decay by the exonuclease XRN1 is a viral mimicry dependency in cancer

  Amir Hosseini, et al., bioRxiv - Cell Biology 2023

  Quote: ... F(ab’)2 Fragment (Alexa Fluor 647 Conjugate (Cell Signaling Technology, 4410) for 1 hour at room temperature ...
• RhoB promotes Salmonella survival by regulating autophagy

  Marco Kirchenwitz, et al., bioRxiv - Cell Biology 2023

  Quote: ... RhoC (3430, 1:1000 dilution, RRID: AB_2179246, Cell Signaling Technology, Danvers, MA, USA), caspase-3 (9662 ...
• RhoB promotes Salmonella survival by regulating autophagy

  Marco Kirchenwitz, et al., bioRxiv - Cell Biology 2023

  Quote: ... AKT (9272, 1:1000 dilution, RRID: AB_329827, Cell Signaling Technology), phospho-AKT (Ser473 ...
• RhoB promotes Salmonella survival by regulating autophagy

  Marco Kirchenwitz, et al., bioRxiv - Cell Biology 2023

  Quote: ... Myc-Tag (2276, 1:1000 dilution, RRID: AB_331783, Cell Signaling Technology, Danvers, MA, USA), RhoA (sc-418 ...
• RhoB promotes Salmonella survival by regulating autophagy

  Marco Kirchenwitz, et al., bioRxiv - Cell Biology 2023

  Quote: ... phospho-AKT (Ser473) (9271, 1:1000 dilution, RRID: AB_329825, Cell Signaling Technology), LC3A/B (4108 ...
• RhoB promotes Salmonella survival by regulating autophagy

  Marco Kirchenwitz, et al., bioRxiv - Cell Biology 2023

  Quote: ... cleaved-caspase-3 (9661, 1:1000 dilution, RRID: AB_2341188, Cell Signaling Technology), AKT (9272 ...
• RhoB promotes Salmonella survival by regulating autophagy

  Marco Kirchenwitz, et al., bioRxiv - Cell Biology 2023

  Quote: ... caspase-3 (9662, 1:1000 dilution, RRID: AB_331439, Cell Signaling Technology), cleaved-caspase-3 (9661 ...
• Frizzled-9 activates YAP to rescue simulated microgravity induced osteoblasts dysfunction

  Qiusheng Shi, et al., bioRxiv - Cell Biology 2023

  Quote: ... pGSK3β (1:2000, Cell Signaling Technology, USA), β-catenin (1:2000 ...
• The effect of estradiol during the early stages of osteoclast differentiation is associated with the accumulation of phosphorylated p53 in mitochondria and the inhibition of mitochondrial metabolism

  Adriana Marques-Carvalho, et al., bioRxiv - Cell Biology 2023

  Quote: ... and goat anti-rabbit IgG (1:5000, CS7074, Cell Signaling, Danvers, MA, USA) secondary antibodies for 1 h at room temperature ...
• Mitochondrial H₂O₂ release does not directly cause genomic DNA damage

  Daan M.K. van Soest, et al., bioRxiv - Cell Biology 2023

  Quote: ... Chk2 (Cell Signaling, CS3440), γH2AX (Millipore ...
• The effect of estradiol during the early stages of osteoclast differentiation is associated with the accumulation of phosphorylated p53 in mitochondria and the inhibition of mitochondrial metabolism

  Adriana Marques-Carvalho, et al., bioRxiv - Cell Biology 2023

  Quote: ... Bax (1:1000, 2772, Cell Signaling, Danvers, MA, USA), denaturated form of OXPHOS complexes cocktail (1:1000 ...
• Mitochondrial H₂O₂ release does not directly cause genomic DNA damage

  Daan M.K. van Soest, et al., bioRxiv - Cell Biology 2023

  Quote: Antibodies used for Western Blot were pChk1 S345 (Cell Signaling, CS2348), Chk1 (Santa Cruz ...
• Proteostasis and lysosomal quality control deficits in Alzheimer’s disease neurons

  Ching-Chieh Chou, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-LC3B (1:200; Cell Signaling, 2775). ...
• Proteostasis and lysosomal quality control deficits in Alzheimer’s disease neurons

  Ching-Chieh Chou, et al., bioRxiv - Cell Biology 2023

  Quote: ... and anti-Vimentin (1:400; Cell Signaling, 5741). Fibroblasts were washed with PBS three times and incubated with fluorophore-conjugated secondary antibody solution in the dark for 1 hr at room temperature ...
• Identification and Characterisation of Proteins Binding to a G-Quadruplex Origin G-rich Repeated Element in Mammalian Cells

  James R. A. Hutchins, et al., bioRxiv - Cell Biology 2023

  Quote: ... incubated in HRP-conjugated anti-IgG secondary antibodies (Cell Signaling Technology) in TBSTM ...
• Frizzled-9 activates YAP to rescue simulated microgravity induced osteoblasts dysfunction

  Qiusheng Shi, et al., bioRxiv - Cell Biology 2023

  Quote: ... pAkt (1:800, Cell Signaling Technology, USA), GAPDH (1:1000 ...
• Frizzled-9 activates YAP to rescue simulated microgravity induced osteoblasts dysfunction

  Qiusheng Shi, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-YAP (1:200, Cell Signaling Technology, USA) and anti-paxillin (1:200 ...
• A new ferrocene derivative blocks KRAS localization and function by oxidative modification at His95

  Kristen M. Rehl, et al., bioRxiv - Cell Biology 2023

  Quote: The following antibodies to measure Ras signaling were purchased from Cell Signaling Technology (Danvers, MA): pAkt ((Ser473 ...
• Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitis

  Masaru Sasaki, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-phospho-SMAD1/5/9 (1:1000;13820S; Cell Signaling Technology, Danvers, MA, USA), and anti-β-actin (1:5000 ...
• Quantitative proteomics and phosphoproteomics of PPP2R5D variants reveal deregulation of RPS6 phosphorylation through converging signaling cascades

  KA Smolen, et al., bioRxiv - Cell Biology 2023

  Quote: ... and P-Ribosomal Protein S6 (S240:244) (5364) were purchased from Cell Signaling Technology.
• The mitotic surveillance pathway requires PLK1-dependent 53BP1 displacement from kinetochores

  Matteo Burigotto, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-53BP1 (Cell Signaling Technology, 4937, 1:500), mouse anti-cyclin A2 (Abcam ...
• The mitotic surveillance pathway requires PLK1-dependent 53BP1 displacement from kinetochores

  Matteo Burigotto, et al., bioRxiv - Cell Biology 2023

  Quote: ... The following antibodies were used: rabbit anti-CENPF (Cell Signaling Technology, 58982,1:500), rabbit anti-53BP1 (Cell Signaling Technology ...
• The mitotic surveillance pathway requires PLK1-dependent 53BP1 displacement from kinetochores

  Matteo Burigotto, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-CENPF (Cell Signaling Technology, 58982,1:500), human anti-centromere/KT (Antibodies Inc ...
• Mitochondrial H₂O₂ release does not directly cause genomic DNA damage

  Daan M.K. van Soest, et al., bioRxiv - Cell Biology 2023

  Quote: ... pChk2 T68 (Cell Signaling, CS2661), Chk2 (Cell Signaling ...
• The effect of estradiol during the early stages of osteoclast differentiation is associated with the accumulation of phosphorylated p53 in mitochondria and the inhibition of mitochondrial metabolism

  Adriana Marques-Carvalho, et al., bioRxiv - Cell Biology 2023

  Quote: ... Membranes were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000, CS7076, Cell Signaling, Danvers, MA, USA) and goat anti-rabbit IgG (1:5000 ...
• Generation and characterization of a Dkk4-Cre knock-in mouse line

  Houda Khatif, Hisham Bazzi, bioRxiv - Developmental Biology 2023

  Quote: ... for 1 h at RT and mounted with Prolong™ Gold Antifade reagent (Cell Signaling Technology, Danvers, MA, USA). For whole- mount staining ...
• Arf1-PI4KIIIβ positive vesicles regulate phosphatidylinositol-3-phosphate signalling to facilitate the fission of lysosomal tubules

  Maxime Boutry, et al., bioRxiv - Cell Biology 2023

  Quote: ... Rabbit anti-β-Actin HRP conjugated (Cell signaling, 5125, dilution 1:10 000), rabbit anti-E ...
• Tissue-intrinsic Wnt signals antagonize Nodal-driven AVE differentiation

  Sina Schumacher, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-pERM (Cell Signaling Technology #3141, 1:200), anti-PODXL (R&D MAB1556 ...
• Proteostasis and lysosomal quality control deficits in Alzheimer’s disease neurons

  Ching-Chieh Chou, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-Nup98 (1:500; Cell Signaling, 2598), anti-p62/SQSTM1 (1:400 ...
• Proteostasis and lysosomal quality control deficits in Alzheimer’s disease neurons

  Ching-Chieh Chou, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-LAMP1 (1:2000; Cell Signaling, 9091), anti-LAMP2 (1:200 ...
• Apical-driven cell sorting optimised for tissue geometry ensures robust patterning

  Prachiti Moghe, et al., bioRxiv - Developmental Biology 2023

  Quote: ... bi-phosphorylated myosin regulatory light chain (ppMRLC) (Cell Signaling, 3674), and Laminin (Novus Biologicals ...
• Mitochondrial DNA Variations Modulate Alveolar Epithelial Mitochondrial Function and Oxidative Stress in Newborn Mice Exposed to Hyperoxia

  Jegen Kandasamy, et al., bioRxiv - Developmental Biology 2023

  Quote: ... with β-Actin (Cell Signaling Technology, 5125S) as loading control ...
• SPLIT HAND/FOOT VARIANTS FAIL TO RESCUE PRDM1A MUTANT CRANIOFACIAL DEFECTS

  Brittany T. Truong, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and incubated overnight at 4°C with rotation in the respective antibody (IgG [1:100; Jackson ImmunoResearch, 111-005-003, RRID: AB_2337913], H3K27ac [1:66; Cell Signaling Technology, 4353S ...
• Apical-driven cell sorting optimised for tissue geometry ensures robust patterning

  Prachiti Moghe, et al., bioRxiv - Developmental Biology 2023

  Quote: ... SOX2 (Cell Signaling, 23064), bi-phosphorylated myosin regulatory light chain (ppMRLC ...
• Parallel genome-scale CRISPR screens distinguish pluripotency and self-renewal

  Bess P. Rosen, et al., bioRxiv - Developmental Biology 2023

  Quote: ... with proteinase/phosphatase inhibitors (5872, Cell Signaling Technology) and 1 mM PMSF (ICN19538105 ...
• Parallel genome-scale CRISPR screens distinguish pluripotency and self-renewal

  Bess P. Rosen, et al., bioRxiv - Developmental Biology 2023

  Quote: Cell pellets were snap frozen in liquid nitrogen and lysed in cell lysis buffer (9803, Cell Signaling Technology) with proteinase/phosphatase inhibitors (5872 ...
• Wnt/β-catenin signaling controls mouse eyelid growth by mediating epithelial-mesenchymal interactions

  Xuming Zhu, et al., bioRxiv - Developmental Biology 2023

  Quote: ... LEF1 antibody (#2230s, Cell Signaling Technology), KRT5 antibody (#905501 ...
• Fetal manipulation of maternal metabolism is a critical function of Igf2 imprinting

  Jorge Lopez-Tello, et al., bioRxiv - Developmental Biology 2023

  Quote: ... portions of the entire pancreas were sectioned at 5μm thickness and ≥3 non-consecutive sections of each portion were stained with an antibody against insulin (1:100, Cell Signaling Technologies: 4590, Danvers, MA, USA), which was subsequently detected by biotinylated goat anti-rabbit secondary antibody (1:1000 ...