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• 5-HT_1A and 5-HT_2B receptor interaction and co-clustering regulates serotonergic neuron excitability

  Amina Benhadda, et al., bioRxiv - Neuroscience 2023

  Quote: ... or mouse anti-myc (1:1,000; Cell Signaling#2276, RRID AB_331783). Secondary antibodies (1:10,000 ...
• A Consequence of Immature Breathing induces Persistent Changes in Hippocampal Synaptic Plasticity and Behavior: A Role of Pro-Oxidant State and NMDA Receptor Imbalance

  Alejandra Arias-Cavieres, Alfredo J. Garcia III, bioRxiv - Neuroscience 2023

  Quote: ... anti-rabbit GluN2B (1:2000; Cell Signaling Technology Cat# 14544, RRID:AB_2798506), anti-rabbit NOX2 (1:2000 ...
• A Consequence of Immature Breathing induces Persistent Changes in Hippocampal Synaptic Plasticity and Behavior: A Role of Pro-Oxidant State and NMDA Receptor Imbalance

  Alejandra Arias-Cavieres, Alfredo J. Garcia III, bioRxiv - Neuroscience 2023

  Quote: ... anti-rabbit GluN2A (1:2000; Cell Signaling Technology Cat# 4205, RRID:AB_2112295), anti-rabbit GluN2B (1:2000 ...
• Riluzole suppresses growth and enhances response to endocrine therapy in ER+ breast cancer

  Ayodeji O. Olukoya, et al., bioRxiv - Cancer Biology 2023

  Quote: ... cleaved caspase 3 (1:300, #9661, Cell Signaling Technology, Danvers, MA), and Ki67 (1:500 ...
• SARM1 detection in oligodendrocytes but not Schwann cells though sarm1/Sarm1 deletion does not perturb CNS nor PNS myelination in zebrafish and mice

  Shaline V. Fazal, et al., bioRxiv - Neuroscience 2022

  Quote: ... Anti-rabbit HRP-linked antibody (Cell Signaling Technology, 1:2000, 7074S), Goat pAb to Chicken IgY H+L (HRP ...
• LIFR recruits HGF-producing neutrophils to promote liver injury repair and regeneration

  Yalan Deng, et al., bioRxiv - Cell Biology 2023

  Quote: ... phospho- Stat3 (Tyr705) (1:1,000, Cell Signaling Technology, 9145S, RRID: AB_2491009), and Stat3 (1:1,000 ...
• Propionate reinforces epithelial identity and reduces aggressiveness of non-small cell lung carcinoma via chromatin remodelling

  Vignesh Ramesh, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-rabbit Alexa Fluor 488 conjugate (1:200, Cell Signaling, 4412S) and anti-goat Alexa Fluor 488 conjugate (1:200 ...
• Interplay between acetylation and ubiquitination of imitation switch chromatin remodeler Isw1 confers multidrug resistance in Cryptococcus neoformans

  Yang Meng, et al., bioRxiv - Microbiology 2022

  Quote: ... anti-HA (C29F4) rabbit mAb (1:5000 dilution; Cell Signaling Technology), anti-Histone H3 (D1H2 ...
• Transcription regulation of SARS-CoV-2 receptor ACE2 by Sp1: a potential therapeutic target

  Hui Han, et al., bioRxiv - Microbiology 2023

  Quote: ... or anti-HNF4α antibodies (3113, 1:1000 dilution, Cell Signaling Technology) overnight at 4 °C ...
• Epoxyeicosatrienoic acids and sEH inhibition prevent cardiac dysfunction in CVB3-induced myocarditis by positively regulating type I interferon signaling

  Zhou Zhou, et al., bioRxiv - Immunology 2023

  Quote: ... phospho-IRF3 (Ser396) (1:1000, 29047s, Cell Signaling Tech, MA, US), TBK1(1:1000 ...
• Transient EZH2 suppression by Tazemetostat during in vitro expansion maintains T cell stemness and improves adoptive T cell therapy

  Yingqin Hou, et al., bioRxiv - Immunology 2023

  Quote: ... anti-H3 (1:1000, Cell Signaling Technology, Cat#4395S, clone: C36B11)) at 4 °C overnight ...
• Transcription regulation of SARS-CoV-2 receptor ACE2 by Sp1: a potential therapeutic target

  Hui Han, et al., bioRxiv - Microbiology 2023

  Quote: ... anti-phospho-AKT (Ser473) (4060, 1:1000 dilution, Cell Signaling Technology), anti-pan-AKT (4691 ...
• Epoxyeicosatrienoic acids and sEH inhibition prevent cardiac dysfunction in CVB3-induced myocarditis by positively regulating type I interferon signaling

  Zhou Zhou, et al., bioRxiv - Immunology 2023

  Quote: ... phospho-TBK1(Ser172) (1:1000, 5483, Cell Signaling Tech, MA, US), GSK3β(1:1000 ...
• Exploiting effector - host interactions using TurboID-based proximity labeling

  Wei Shi, et al., bioRxiv - Plant Biology 2022

  Quote: ... and anti-rabbit lgG secondary antibody (1:3,000 dilution; Cell Signaling). The membranes were probed with primary antibody for two hours or overnight and secondary antibody for one hour ...
• Human assembloids reveal the consequences of CACNA1G gene variants in the thalamocortical pathway

  Ji-il Kim, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-OCT4 (C30A3) (rabbit, Cell Signaling Technology, 2840, 1:200 dilution) diluted in PBS containing 2% NDS and 0.1% Triton X-100 ...
• The cancer-specific lncRNA LISR customizes ribosomes to suppress anti-tumour immunity

  Sonia Cinque, et al., bioRxiv - Cancer Biology 2023

  Quote: ... eIF2α-Phospho-S51 (#3398, clone D9G8; Cell Signaling Technology, 1:800), α-O-Linked N-Acetylglucosamine (MABS157 ...
• Calcium signals tune AMPK activity and mitochondrial homeostasis in dendrites of developing neurons

  Akane Hatsuda, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-phospho-ULK1 (Ser555) (1:1000, Cell Signaling Technology, 5869); rabbit anti-PGC1α (1:5000 ...
• Peroxisomal tail-anchored proteins do not reach peroxisomes via ER, instead mitochondria can be involved

  Tamara Somborac, et al., bioRxiv - Cell Biology 2023

  Quote: ... Primary antibodies: rabbit anti-GFP 1:2500 (Cell Signaling Technology, 2956), rabbit anti-PEX19 1:1000 (Abcam ...
• Dynamic Effects of Ventral Hippocampal NRG3/ERBB4 Signaling on Nicotine Withdrawal-Induced Responses

  Miranda L. Fisher, et al., bioRxiv - Neuroscience 2023

  Quote: ... and Beta-tubulin (1:2000, 2128L, Cell Signaling Technology, Danvers, MA). After washing in phosphate-buffered saline-Tween-20 (PBST) ...
• Tuned geometries of hippocampal representations meet the demands of social memory

  Lara M. Boyle, et al., bioRxiv - Neuroscience 2023

  Quote: ... or STEP (1:1000, mouse anti-STEP # 4396, Cell Signaling Technology) at 4°C in 0.1% Triton-X in PBS plus 5% goat serum ...
• SGC-CLK-1 (CAF-170) a chemical probe for the Cdc2-Like kinases CLK1, CLK2, and CLK4

  Deanna Tiek, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and anti–α/β-tubulin (1:1000, Cell Signaling Technology, 2148) antibodies ...
• A Potent and Selective CDKL5/GSK3 Chemical Probe is Neuroprotective

  Han Wee Ong, et al., bioRxiv - Cell Biology 2023

  Quote: ... and rabbit anti phospho-β-catenin (1:250, Cell Signaling 9561). The following secondary antibodies were used at a concentration of 1:10,000 ...
• 5-HT_1A and 5-HT_2B receptor interaction and co-clustering regulates serotonergic neuron excitability

  Amina Benhadda, et al., bioRxiv - Neuroscience 2023

  Quote: ... or mouse anti-myc (1:1,000; Cell Signaling#2276, RRID AB_331783). Antibodies (1:10,000 ...
• Spatial and temporal transcriptomics of SHH-medulloblastoma with chromothripsis identifies multiple genetic clones that resist to treatment and lead to relapse

  Ilia Kats, et al., bioRxiv - Cancer Biology 2023

  Quote: ... incubation of the primary antibodies Ki67 (#11882, Cell Signaling, 1:50) and NeuroD1 (#AF2746 ...
• GTP signaling links metabolism, DNA repair, and responses to genotoxic stress

  Weihua Zhou, et al., bioRxiv - Cancer Biology 2023

  Quote: ... p-γ-H2AX (1:1000 dilution; Cat# 9718, Cell Signaling Technology) at 4 °C overnight ...
• Gut barrier defects, increased intestinal innate immune response, and enhanced lipid catabolism drive lethality in N-glycanase 1 deficient Drosophila

  Ashutosh Pandey, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-pAkt 1:1000 (cat No. 4060, Cell Signaling technology) and mouse anti-actin 1:1000 (DSHB Cat ...
• Virally induced lipid droplets are a platform for innate immune signalling complexes

  EA Monson, et al., bioRxiv - Immunology 2023

  Quote: ... rabbit monoclonal αPhospho-STAT1 (Tyr701) (1:1000; #7649, Cell Signaling Technology), rabbit polyclonal αmCherry (1:1000 ...
• NRF2 connects Src tyrosine kinase to ferroptosis resistance in glioblastoma

  Claudia Cirotti, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-phospho-ERK 1/2 (Thr202/Tyr204) (D13.14.4E) (Cell Signaling Technology), anti-Ubiquitin (P4D1 ...
• Zmiz1 is a novel regulator of lymphatic endothelial cell gene expression and function

  Rajan K C, et al., bioRxiv - Molecular Biology 2023

  Quote: ... p44/42 MAPK (ERK1/2) (Cell Signaling Technology, 4695S, 1:1,000), p-p44/42 MAPK (pERK1/2 ...
• Electrophysiology of human iPSC-derived vascular smooth muscle cells and cell autonomous consequences of Cantu Syndrome mutations

  Alex Hanson, et al., bioRxiv - Physiology 2023

  Quote: ... rabbit anti-α-SMA (1:100; D4K9N, XP; Cell Signaling Technology), and rabbit anti-TAGLN (1:100 ...
• Hemin decreases cellular aging and enhances healthspan and lifespan through the AMPK pathway

  Yizhong Zhang, et al., bioRxiv - Genetics 2023

  Quote: ... and anti-AMPKα antibody (1:1000 dilution; D5A2; Cell Signaling Technology), respectively ...
• A large-scale sORF screen identifies putative microproteins and provides insights into their interaction partners, localisation and function

  Dörte Schlesinger, et al., bioRxiv - Molecular Biology 2023

  Quote: ... anti-β-Actin 1:4000 (13E5, Cell Signaling 4970, rabbit monoclonal). Secondary antibodies as follows ...
• The universal suppressor mutation in the HSV-1 nuclear egress complex restores membrane budding defects by stabilizing the oligomeric lattice

  Elizabeth B. Draganova, et al., bioRxiv - Biophysics 2023

  Quote: ... or rabbit antibody to calnexin (diluted 1:1000) (Cell Signaling Technology).
• APOE4/4 is linked to damaging lipid droplets in Alzheimer’s microglia

  Michael S. Haney, et al., bioRxiv - Neuroscience 2023

  Quote: ... and mouse anti-ý-amyloid (1:500; Cell Signaling Technologies, #15126). After primary antibody incubation ...
• Genetically perturbed myelin as a risk factor for neuroinflammation-driven axon degeneration

  Janos Groh, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-c-Jun (1:300, catalog no. 9165; Cell Signaling); rat anti-MHC-I (1:100 ...
• Anti-apoptotic and anti-inflammatory properties of grapefruit IntegroPectin on human microglial HMC3 cell line

  Miriana Scordino, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-p-ERK1/2 (1:1000, 9101 Cell Signaling Technology), rabbit anti-p-Akt (1:1000 ...
• Molecular signatures of hyperexcitability and lithium responsiveness in bipolar disorder patient neurons provide alternative therapeutic strategies

  Anouar Khayachi, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit monoclonal anti-phospho-Akt (Ser473) (1/1000; Cell Signaling #4060); rabbit monoclonal anti-phospho-AMPKβ1 (Ser108 ...
• 3D human retinal organoid model for the study of early diabetic retinopathy

  Luisa de Lemos, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-S6 Ribosomal Protein (1:1000, 2217, Cell Signaling Technology), mouse anti-β-Actin-peroxidase (1:25000) ...
• RNA-mediated ribonucleoprotein assembly controls TDP-43 nuclear retention

  Patricia M. dos Passos, et al., bioRxiv - Cell Biology 2023

  Quote: ... and immunostained with rabbit anti-HA (1:1500, Cell Signaling Technologies) overnight at 4°C in 10% NGS/PBS ...
• Inhibition of Cellular MEK/ERK Signaling Suppresses Murine Papillomavirus Type 1 Replicative Activities and Promotes Tumor Regression

  Adrian J. Luna, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Immunohistochemical detection of p-ERK1/2 (Cell Signaling #4370; 1:500) required the tyramide signal amplification (TSA ...
• Increased levels of eIF2A inhibit translation by sequestering 40S ribosomal subunits

  Daisy J. Grove, et al., bioRxiv - Biochemistry 2023

  Quote: ... Rabbit anti-GAPDH was used at 1:1,000 (Cell Signaling # 5174S). Rabbit anti-RPS3 (Bethyl # A303-840A ...
• Urothelium-specific expression of mutationally activated Pik3ca initiates early lesions of non-invasive bladder cancer

  Lauren Shuman, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rabbit anti-phospho-Akt (T308) (1:200; 13038; Cell Signaling Technology), goat anti-Foxa1 (1:1000 ...
• A Conserved Core Region of the Scaffold NEMO is Essential for Signal-induced Conformational Change and Liquid-liquid Phase Separation

  Christopher J. DiRusso, et al., bioRxiv - Molecular Biology 2023

  Quote: ... p65 or anti-mouse-HRP (1:3000, Cell Signaling Technology 7076) for anti-phospho-IκBα ...
• DENND6A couples Arl8b to a Rab34/RILP/dynein complex regulating retrograde lysosomal trafficking and autophagy

  Rahul Kumar, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-VDAC antibody (4866S) is from Cell Signaling (WB-1:1000), anti-Catalase antibody (D4P7B ...
• DENND6A couples Arl8b to a Rab34/RILP/dynein complex regulating retrograde lysosomal trafficking and autophagy

  Rahul Kumar, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-LC3B antibody (3868) is from Cell Signaling (WB-1:1000), anti-RILP antibody (13574-1-AP ...
• A high-content screen identified ingenol-3-angelate as an enhancer of B7-H3-CAR T cell activity by increasing B7-H3 protein expression on the target cell surface via PKCα activation

  Ha Won Lee, et al., bioRxiv - Cancer Biology 2023

  Quote: ... or 1:1000 anti-PKC-alpha antibody (Cell Signaling Technology, 2056S) with or without 1:10,000 anti-beta actin (Sigma ...
• Robust axis elongation by Nodal-dependent restriction of BMP signaling

  Alexandra Schauer, et al., bioRxiv - Developmental Biology 2023

  Quote: ... α-pSmad5 and α-pSmad2 (1:1000, cat.no. 8828, Cell Signaling Technology) double WMIF was performed as described in (Soh et al ...
• DNAJB1-PRKACA fusion protein-regulated LINC00473 promotes tumor growth and alters mitochondrial fitness in fibrolamellar carcinoma

  Rosanna K. Ma, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and rabbit IgG secondary antibody (1:2000, Cell Signaling Technology, 7074). Protein quantification was performed using FIJI software (normalized to vinculin ...
• Inhibition of OCT4 Binding at the MYCN Locus Induces Neuroblastoma Cell Death Accompanied by Downregulation of Transcripts with High-Open Reading Frame Dominance

  Kazuma Nakatani, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-p53 (1:1000 dilution; #2524, Cell Signaling Technology, Danvers, MA), anti-caspase-2 (1:1000 dilution ...
• PET imaging of fibroblast activation protein alpha (FAP) detects incipient cardiotoxicity due to anthracycline chemotherapy

  Chul-Hee Lee, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and anti-HSP60 (1:1000 dilution, 12165S, Cell Signaling Technology, USA). The chemical luminescent signals were measured by Azure c400 Gel imaging system (Azure Biosystems ...