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Citations for Cell Signaling Technology :
851 - 900 of 1024 citations for 3 3 Bromomethyl phenyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Tissue (n=3 tendon per sample) was homogenized in RIPA buffer (including 1X protease/phosphatase inhibitor, Cell Signaling #58725) to extract the total protein with the Biomasher II system and Powermasher II electric stirrer (Nippi Inc ...
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bioRxiv - Neuroscience 2022Quote: ... Samples from nuclear fractions were incubated with antibodies for nuclear loading control histone 3 (H3; 1:1000, Cell Signaling). Whole-cell tissue samples were incubated with antibodies for phospho-MAPK (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... rat TS cells were exposed to a phosphatidylinositol 3-kinase (PI3K) inhibitor (LY294002, 10 μM, 9901, Cell Signaling Technology), chloroquine (50 μM for 24 h ...
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bioRxiv - Molecular Biology 2024Quote: The following primary antibodies were used: anti-cleaved caspase-3 (Asp175) (rabbit polyclonal, #9661, RRID: AB_2341188, Cell Signaling Technology, Danvers ...
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bioRxiv - Developmental Biology 2024Quote: ... the blocking solution was drained and replaced with primary antibody: Cleaved Caspase-3 (1:250, Cell Signaling cat#9661T), or αPhospho-histone H3 (Ser10 ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were treated with primary antibody against cleaved-caspase 3 (1:300, 9661S; Cell Signaling Technology, Danvers, MA) and incubated at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were rinsed 3 times with PBS for 5 minutes before adding fluorescent secondary antibody (Cell Signaling Technology 4413S) diluted 1:2000 in PBS containing 1% BSA and 0.1% Triton-X at 37°C for 25 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... immunoprecipitation assays were performed for 3-h at 4°C using anti-cMyc (Cell Signaling technology, catalog no. 2276), anti-HA (Cell Signaling technology ...
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bioRxiv - Cancer Biology 2024Quote: ... 1-3 million cells were lysed with IP lysis buffer including protease/phosphatase inhibitor cocktail (Cell Signaling Technology, 5872) for 10 mins ...
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bioRxiv - Biophysics 2024Quote: ... Membranes were then washed 3 times with TBST and incubated for 1h with the anti-mouse (#7076, Cell Signaling) or anti-rabbit (#7074 ...
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bioRxiv - Cell Biology 2024Quote: ... Then organoids were incubated overnight at 4°C in 1:400 anti-Cleaved caspase 3 (CC3; Cell Signaling; 9661S). Organoids were rinsed 3 x 2 hours in OWB on a rocking platform before incubating overnight at 4°C in 1:250 donkey anti-rabbit Alexa Fluor 555 (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... probed for ACSS2 with anti-AceCS1 (1:50 dilution in 3% BSA in PBS, clone: D19C6, Cell Signaling Technology), detected with FITC-conjugated AffiniPure Donkey Anti-Rabbit IgG (1:1000 dilution in 3% BSA in PBS ...
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bioRxiv - Developmental Biology 2024Quote: Rabbit polyclonal antibodies to cleaved caspase-3 and to GAPDH were purchased from Cell Signaling Technologies (Danvers, MA, USA). Mouse monoclonal antibodies to GFAP ...
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bioRxiv - Cell Biology 2020Quote: ... The 6E2 mouse IgG anti-hemagglutinin tag and rabbit IgG anti-caspase 3 were from Cell Signaling (Danvers, MA, USA). The anti-CyPA goat polyclonal (cat #PA5-18463 ...
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bioRxiv - Cancer Biology 2021Quote: ... underwent antigen retrieval and were incubated with primary antibodies (KIT, DakoCytomation/Agilent; Ki-67, GeneTex; cleaved caspase 3, Cell Signaling). The signal was detected using biotin-conjugated secondary antibodies (Abcam) ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were eluted by precipitating beads on a magnetic rack and resuspending in 40 μL of 3× blue sample buffer 30× reducing agent: 1.25 M DTT (1:30; 7722s, Cell Signaling). Resulting samples were heated at 95°C (5 min) ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunohistochemistry was performed on 5-μm thick sections using antibodies against mouse cleaved-caspase 3 (Cell Signaling #9661S, 1:400) and Ki67 (Thermo RM-9106-S ...
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bioRxiv - Molecular Biology 2020Quote: ... Apoptosis in the plaque was analyzed by staining the plaques with anti-cleaved caspase 3 (1:100, 9664, Cell signaling). Cell proliferation was analyzed by staining the plaques for Ki67 (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were incubated overnight in primary antibody diluted in blocking buffer (1:1000 anti-cleaved caspase-3 Cell Signaling Technologies #9664 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with Perm/Wash buffer twice before stained with rabbit anti-Cleaved Caspase 3 (Asp175) (Cell Signaling Technologies) at a 1:200 dilution for 30 mins at room temperature and was detected with anti-rabbit BV421 (Dnk ...
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bioRxiv - Cell Biology 2020Quote: ... was applied and the sections were washed 3 times and were detected using the DAB method (#80259P, Cell Signaling Technology). Standard hematoxylin and eosin staining was performed on rehydrated fat paraffin sections ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... Slides were then incubated at 4°C overnight with the corresponding primary antibody (anti-acetylated tubulin, Sigma T7451, mouse monoclonal, 1/60,000 dilution; anti-cleaved caspase 3, Cell Signaling 9579 ...
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bioRxiv - Microbiology 2023Quote: Cell lysis was performed using Radioimmunoprecipitation (RIP) assay buffer (#9806, Cell Signaling Technology, 3 Trask Lane, Danvers, MA 01923, USA) supplemented with protease and phosphatase inhibitor (#5872) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked in 2 % goat or donkey anti-serum in KPBS and 3 % BSA plus 0.1 % Triton X-100 and incubated with primary antibody (rabbit anti-c-Fos, 1:200, Cell Signaling, Danvers ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary Antibody cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 647 Conjugate) (Cell Signaling Technology, Danvers, MA, USA)
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bioRxiv - Developmental Biology 2022Quote: ... To stain and detect the mitotic cells in the tissue, polyclonal rabbit phospho-histone 3 (pH3, ser10) antibody (Cell Signaling) together with Alexa Fluor 647 conjugated anti-rabbit secondary antibody (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were next incubated with rabbit active caspase-3 primary antibody (0.768 ng/mL, Cell Signaling Technology, Leiden, The Netherlands), followed by incubation in goat anti-mouse Texas Red secondary antibody (20 μg/mL in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated overnight at 4°C with the following primary antibodies diluted in PHEM/0.1% Triton/3% BSA: anti-NANOG (D73G4; 1:200, #mAb 4903, Cell Signaling), anti-OCT4 (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were incubated with 0.5 ml PBS BSA 3% (A1391, Applichem) with primary antibodies (mouse-anti-HA monoclonal antibodies (C29F4, Cell Signaling) at 1:500 ...
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bioRxiv - Immunology 2024Quote: ... HDAC1 activity was measured by ELISA assay detecting the level acetylated Histone 3 (anti-acetil-H3; K27; 8173S; Cell Signaling).
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bioRxiv - Cancer Biology 2024Quote: ... 5 µl SureBeads were washed in 0.1% TBST for 3 times and then incubated with 1:50 ACSS2 primary antibody (Rabbit, Cell Signaling) or 1:50 IgG control (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissues were stained with Alexa Fluor 488 anti-Cleaved caspase-3 (1:20, clone 5A1E, Cell Signaling Technology, 67308) for 1 hour in the dark.
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bioRxiv - Developmental Biology 2024Quote: The following antibodies were used for the chicken data: rabbit anti-cleaved caspase 3 (CASP3, 1:500, 9661, Cell Signaling); rabbit anti-caveolin 1 (CAV1 ...
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bioRxiv - Developmental Biology 2024Quote: ... on 4% paraformaldehyde-fixed larvae using anti-HCS-1 (otoferlin, Developmental Studies Hybridoma Bank / NICHD, RRID:AB_10804296, 1:500) or anti-cleaved caspase 3 (CC3, Cell Signaling Technology 9661 ...
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bioRxiv - Cancer Biology 2021Quote: ... and sectioned tissues were stained with hematoxylin and eosin or with anti-cleaved caspase 3 (Cell Signaling Technology, #4850, 1:200) and anti-RSK3 antibodies (Novus Biologicals ...
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bioRxiv - Cell Biology 2020Quote: ... The following primary antibodies were incubated at 4°C overnight: Rabbit anti-Cleaved Caspase 3 (Cat# 9661, Cell Signaling Technologies, MA), Rabbit anti-OLFM4 (Cat# 39141T ...
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bioRxiv - Genetics 2021Quote: ... The following antibodies were used in this study: rabbit anti-cleaved caspase 3 D175 at 1:100 (Cell Signaling Technology #9661); Alexa 647 goat anti-HRP at 1:500 (Jackson ImmunoResearch Laboratories Inc ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed (3 × 10 mins) in TBST before and after incubating with a 1:10,000 anti-mouse secondary antibody (Cell Signaling, 7076S) overnight ...
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bioRxiv - Genetics 2021Quote: ... The blots were washed (3 × 10 mins) in TBST before and after incubating with a 1:10,000 anti-rabbit secondary antibody (Cell Signaling, 7074S). The blots were washed overnight before being incubated with a 1:5000 dilution of anti-HSP70-HRP (need catalog number ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed (3 × 10 mins) in TBST before and after incubating with either a 1:10,000 anti-mouse secondary antibody (Cell Signaling, 7076S) or 1:10,000 anti-rabbit secondary antibody (Cell Signaling ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were labeled overnight at 4 °C with the primary antibodies anti-Ki67 (1:200, 275R, Cell Marque) and anti-phosphorylated histone 3 (1:200, 9701, Cell Signaling) for ...
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bioRxiv - Physiology 2022Quote: The acetyl peptide enrichment was performed on 3 mg of digested peptides using PTMscan Acetyl-Lysine Motif kit (Cell Signaling #13416). The peptides were mixed with 100 µL of 10x IP buffer (500 mM MOPS ...
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bioRxiv - Genetics 2022Quote: ... human anticentromere (1:100; Antibodies Inc., cat# 15-234) and mouse monoclonal anti-H3K9me2/3 (1:100, Cell Signaling, cat# 5327) primary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies (Anti-3-hydroxybutyryllysine [PTM Biolabs 1201], anti-p84 [GeneTex GTX70220], anti-α-Tubulin [Sigma T5168], anti-COXIV [Cell Signaling 11967S] ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in 8 well chamber slides and fixed in 4% paraformaldehyde and permeabilized using 0.1% saponin in blocking buffer (HBSS containing 3% BSA, 0.2% gelatin, and 0.02% NaN3) and stained with Rabbit anti-mouse YAP1 (Cell Signaling Technology, D8H1X), FITC-conjugated phalloidin ...
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bioRxiv - Immunology 2021Quote: ... Δagr and in-vitro-transcribed RNAIII (3 µg ml-1, 1 µg ml-1) were lysed in 30 µl of 1X cell lysis buffer (Cell Signaling) supplemented with 1X protease cocktail inhibitor (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times with TBS-T (0.05% v/v Tween-20 in TBS) before incubation with HRP conjugated antibody (Cell Signaling Technology 7076S ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies and dilutions (in TBST for primary or 3% BSA in TBST for secondary) were used for immunofluorescence: E-cadherin (#14472, monoclonal mouse, 1:100, Cell Signaling), mouse-IgG Alexa Fluor 594-conjugated secondary (#8890 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 × 10 min with TBST and incubated for 1 h with the appropriate secondary antibody (Cell Signaling Technologies) diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were incubated with a polyclonal cleaved caspase 3 primary antibody (rabbit, 1:100; 9661, Cell Signaling, Frankfurt am Main, Germany) for 60 min at room temperature followed by incubation with a monoclonal secondary horseradish peroxidase (HRP)-coupled horse-anti-rabbit antibody with AEC+ as chromogen ...