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• A Spatiotemporal Map of Co-Receptor Signaling Networks Underlying B Cell Activation

  Katherine J. Susa, et al., bioRxiv - Immunology 2023

  Quote: ... or Phospho-CD19 (Tyr531) antibody (Cell Signaling, 1:1000 dilution) in TBST containing 1% BSA ...
• MARCH5-dependent NLRP3 ubiquitination is an essential step for NEK7 docking on the mitochondria

  Yeon-Ji Park, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human IL-1β (1:1,000, #12242, Cell Signaling Technology), anti-IL-1β (1:1,000 ...
• A Cas9-fusion proximity-based approach generates an Irak1-Mecp2 tandem duplication mouse model for the study of MeCP2 duplication syndrome

  Eleonora Maino, et al., bioRxiv - Genetics 2023

  Quote: ... rabbit monoclonal D51G7 anti-IRAK1 (#4504, Cell Signaling, 1:1000), rabbit monoclonal anti-GAPDH (sc-47724 ...
• The myocardium utilizes Pdgfra-PI3K signaling to steer towards the midline during heart tube formation

  Rabina Shrestha, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and pan-AKT (1:2000, Cell Signaling 4691, RRID: AB_915783), Anti-rabbit HRP-conjugated (1:5000 ...
• The phosphodiesterase 2A regulates lymphatic endothelial development via cGMP-mediated control of Notch signaling

  Claudia Carlantoni, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-β-actin (Cat#: 4970, Cell Signaling, 1:1000), and anti-PROX1 (Cat# ...
• Intrinsic microtubule destabilization of multiciliated choroid plexus epithelial cells during postnatal lifetime

  Kim Hoa Ho, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-acetylated α-tubulin (1:800, #5335T, Cell Signaling), mouse anti-glutamylated tubulin GT335 (1:800 ...
• Control of gastruloid patterning and morphogenesis by the Erk and Akt signaling pathways

  Evan J. Underhill, Jared E. Toettcher, bioRxiv - Developmental Biology 2023

  Quote: ... Snail C15D3 rabbit (Cell Signaling Technology, 3879, 1:50 dilution), E-cadherin 24E10 rabbit (Cell Signaling Technology ...
• Mob4 is essential for spermatogenesis in Drosophila melanogaster

  Inês B. Santos, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and anti-cleaved caspase 3 (1:500, Cell Signaling #9661). The samples were washed thrice in PBST for 20 min and incubated with appropriate secondary antibodies in a humid chamber for 2 h at room temperature ...
• Dysregulated CD38 expression in blood and skin immune cells of patients with hidradenitis suppurativa

  Peter Dimitrion, et al., bioRxiv - Immunology 2023

  Quote: ... and CD14 (Cell Signaling D7A22T; 1:250, TRS-low pH) on a Dako Autostainer Link ...
• Gestational diabetes mellitus dysregulates the PD-1/PD-L1 axis at the feto-maternal interface

  ZN. Mihalic, et al., bioRxiv - Immunology 2023

  Quote: ... Anti-PD-L1 (clone E1L3N, 1:1000 dilution, Cell Signaling) and anti-GAPDH (clone 14C10 ...
• Gestational diabetes mellitus dysregulates the PD-1/PD-L1 axis at the feto-maternal interface

  ZN. Mihalic, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-GAPDH (clone 14C10, 1:500 dilution, Cell Signaling) monoclonal antibodies were used ...
• Mitochondrial metabolism in Drosophila macrophage-like cells regulates body growth via modulation of cytokine and insulin signaling

  Shrivani Sriskanthadevan-Pirahas, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and anti-phospho-JNK (1:500, Cell Signaling Technolog #4668S). Goat and donkey secondary antibodies were purchased from Santa Cruz Biotechnology (sc-2030 ...
• Oxidative stress-induced MMP- and γ-secretase-dependent VE-cadherin processing is modulated by the proteasome and BMP9/10

  Caterina Ivaldo, et al., bioRxiv - Cell Biology 2022

  Quote: ... and anti-β-catenin (rabbit, Cell Signaling Technology, 1:100) antibodies were used followed by specific secondary Alexa Fluor antibodies (1:500 anti-mouse Alexa Fluor 546 and 1:500 anti-rabbit Alexa 488 Thermo Fisher) ...
• The exon junction complex component EIF4A3 is essential for mouse and human cortical progenitor mitosis and neurogenesis

  Bianca M. Lupan, et al., bioRxiv - Developmental Biology 2023

  Quote: ... as well as Ki67 (Cell Signaling Technology, 12202, 1:250).
• Partial Activation of PPAR-γ by Synthesized Quercetin Derivatives Modulates TGF-β1-Induced EMT in Lung Cancer Cells

  Sangeeta Ballav, Amit Ranjan, Soumya Basu, bioRxiv - Cancer Biology 2022

  Quote: ... and anti-Zeb-1 (rabbit, Cell Signaling Technology, CA, USA). Anti-rabbit and anti-mouse antibodies conjugated to horseradish peroxidase (HRP ...
• A multifunctional Wnt regulator underlies the evolution of coat pattern in African striped mice

  Matthew R. Johnson, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Primary antibodies rabbit anti-LEF1 (Cell signaling #2230S, 1:100), rabbit anti-β-catenin (Sigma # C2206 ...
• Spatiotemporal analysis of SARS-CoV-2 infection reveals an expansive wave of monocyte-derived macrophages associated with vascular damage and virus clearance in hamster lungs

  Ola Bagato, et al., bioRxiv - Immunology 2023

  Quote: ... rabbit anti-Cleaved Caspase-3 (Cell Signaling, #9661, 1:200), rabbit anti-Uteroglobin (Abcam ...
• Paternal methamphetamine exposure induces higher sensitivity to methamphetamine in male offspring through driving ADRB1 on CaMKII-positive neurons in mPFC

  Yanyan Zheng, et al., bioRxiv - Neuroscience 2023

  Quote: ... Rabbit anti-CREB (1:1000, RRID:AB_2800317, Cell Signaling Technology, USA), Rabbit anti-phospho-CREB (1:1000 ...
• Regulation of Store-Operated Ca²⁺ Entry by IP₃ Receptors Independent of Their Ability to Release Ca²⁺

  Pragnya Chakraborty, et al., bioRxiv - Cell Biology 2023

  Quote: ... STIM1 (1:1000, Cell Signaling Technology, Cat# 5668S, RRID: AB_10828699); Orai1 (1:500 ...
• Regulation of Store-Operated Ca²⁺ Entry by IP₃ Receptors Independent of Their Ability to Release Ca²⁺

  Pragnya Chakraborty, et al., bioRxiv - Cell Biology 2023

  Quote: ... STIM1 (1:1000, Cell Signaling Technology, Cat# 5668S, RRID: AB_10828699); and Orai1 (1:500 ...
• Distinct Nrf2 Signaling Thresholds Mediate Lung Tumor Initiation and Progression

  Janine M. DeBlasi, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and Cleaved Caspase-3 (Cell Signaling Technology, RRID:AB_2070042, 1:1000). Following overnight incubation at 4°C in primary antibody ...
• Loss of polarity regulators initiates gasdermin E mediated pyroptosis in human maternal fetal interface trophoblasts

  Khushali Patel, et al., bioRxiv - Developmental Biology 2023

  Quote: ... mouse anti-AKT(pan) (1:2000; Cell Signaling Technology #2920), rabbit anti-phospho Ser473 AKT (1:2000 ...
• Loss of polarity regulators initiates gasdermin E mediated pyroptosis in human maternal fetal interface trophoblasts

  Khushali Patel, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-cleaved GSDMD Asp275 (1:500; Cell Signaling Technologies #36425); anti-GSDME (1:50 ...
• Lysosomal cystine export regulates mTORC1 signaling to guide kidney epithelial cell fate specialization

  Marine Berquez, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-S6 Ribosomal Protein (Cell Signaling Technology, 2217, 1:500), anti-phospho‒4E-BP1 (Ser65 ...
• Cellular mechanisms of heterogeneity in NF2-mutant schwannoma

  Christine Chiasson-MacKenzie, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-Akt rabbit mAb (1:1000; 11E7, Cell Signaling Technology); anti-phospho p44/42 (ERK1/2 ...
• Repair oligodendrocytes demyelinating and disintegrating damaged axons after injury

  Gianluigi Nocera, et al., bioRxiv - Neuroscience 2023

  Quote: ... Phospho-c-Jun (Rabbit, 1:500, Cell Signaling, D47G9, # 3270), p44/42 MAPK (Mouse ...
• Repair oligodendrocytes demyelinating and disintegrating damaged axons after injury

  Gianluigi Nocera, et al., bioRxiv - Neuroscience 2023

  Quote: ... p44/42 MAPK (Mouse, 1:1000, Cell signaling, L34F12, #4696), Phospho-p44/42 MAPK (Rabbit ...
• The hepatokine FGL1 regulates hepcidin and iron metabolism during the recovery from hemorrhage-induced anemia in mice

  Ugo Sardo, et al., bioRxiv - Physiology 2023

  Quote: ... or horse anti-mouse IgG (Cell signaling, 7076, 1/10000) secondary antibodies conjugated with HRP and diluted in TBST- NFDM (5%) ...
• Predicting gene level sensitivity to JAK-STAT signaling perturbation using a mechanistic-to-machine learning framework

  Neha Cheemalavagu, et al., bioRxiv - Systems Biology 2023

  Quote: ... and anti-pSTAT1 Y701 (Cell Signaling Technology 9167, 1:400). Cells were then washed and stained for 1 hour at room temperature with the following secondary antibodies ...
• Single Cell Resolution Spatial Mapping of Human Hematopoiesis Reveals Aging-Associated Topographic Remodeling

  Aleksandr Sarachakov, et al., bioRxiv - Systems Biology 2023

  Quote: ... 3) Opal 570/anti-CD117 (1:100, D3W6Y, Cell Signaling), 4 ...
• Ca²⁺/Calmodulin Dependent Protein Kinase Kinase-2 (CaMKK2) promotes Protein Kinase G (PKG)-dependent actin cytoskeletal assembly to increase tumor metastasis

  Debarati Mukherjee, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-phospho-VASP (Ser157) (1:1000, Catalog # 3111, Cell Signaling); anti-VASP (1:2000 ...
• Combinatorial genetic strategy accelerates the discovery of cancer genotype-phenotype associations

  Shan Li, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rabbit monoclonal GATA3 antibody (clone D13C9, Cell Signaling, 1:200); rabbit monoclonal CD44 antibody (clone E7K2Y ...
• Combinatorial genetic strategy accelerates the discovery of cancer genotype-phenotype associations

  Shan Li, et al., bioRxiv - Cancer Biology 2023

  Quote: ... mouse monoclonal p53 antibody (clone 1C12, Cell Signaling, 1:500); rabbit monoclonal HOXB13 antibody (clone D7N8O ...
• Combinatorial genetic strategy accelerates the discovery of cancer genotype-phenotype associations

  Shan Li, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rabbit monoclonal CD44 antibody (clone E7K2Y, Cell Signaling, 1:100).
• Combinatorial genetic strategy accelerates the discovery of cancer genotype-phenotype associations

  Shan Li, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rabbit monoclonal GFP antibody (clone D5.1, Cell Signaling, 1:100); rabbit polyclonal p63 antibody (12143-1-AP ...
• Oligodendrocyte-derived IL-33 functions as a microglial survival factor during neuroinvasive flavivirus infection

  Geoffrey T. Norris, et al., bioRxiv - Immunology 2023

  Quote: ... Rabbit anti-Phospho-c-Jun (1:1000; D47G9; Cell Signaling), and Donkey anti-Mouse IgG (1:2000 ...
• Combinatorial genetic strategy accelerates the discovery of cancer genotype-phenotype associations

  Shan Li, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rabbit monoclonal HOXB13 antibody (clone D7N8O, Cell Signaling, 1:50); rabbit polyclonal AR antibody (06-680 ...
• Global substrate identification and high throughput in vitro dephosphorylation reactions uncover PP1 and PP2A-B55 specificity principles

  Jamin B. Hein, et al., bioRxiv - Cell Biology 2023

  Quote: ... pan T(phos)P –mouse - 1:500 (Cell signaling #9391)
• Progranulin inhibits phospholipase sPLA2-IIA to control neuroinflammation

  Huan Du, et al., bioRxiv - Neuroscience 2023

  Quote: ... mouse anti-GFAP (Cell signaling, 3670S, 1:100 for immunostaining), mouse anti-ACBD3 (Santa Cruz Biotechnology ...
• CHAF1A promotes the translesion DNA synthesis pathway in response to DNA replication stress

  Bing Wen, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit polyclonal anti-RPA2 (35869S, Cell Signaling Technology; 1:1000), mouse monoclonal anti-Pol η (sc-17770 ...
• Periostin facilitates ovarian cancer recurrence by enhancing cancer stemness

  Zhiqing Huang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... including anti-phospho-Akt (1:2500, Cell Signaling, Cat# #9271), anti-Akt (1:1000 ...
• Repair oligodendrocytes demyelinating and disintegrating damaged axons after injury

  Gianluigi Nocera, et al., bioRxiv - Neuroscience 2023

  Quote: ... Phospho-c-Jun (Rabbit, 1:100, Cell Signaling, D47G9, # 3270), Phospho-p44/42 MAPK (Rabbit ...
• Focal adhesions are controlled by microtubules through local contractility regulation

  J. Aureille, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-ARP2 (Cell Signaling, cat. no. #3128, dilution 1:1000); anti-Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197)4 ...
• The adhesion GPCRs CELSR1-3 and LPHN3 engage G proteins via distinct activation mechanisms

  Duy Lan Huong Bui, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-HA rabbit (Cell Signaling Technologies Cat# 3724; 1:2,000), Alexa Fluor 647 Phalloidin (Invitrogen Cat# A22287 ...
• RhoB promotes Salmonella survival by regulating autophagy

  Marco Kirchenwitz, et al., bioRxiv - Cell Biology 2023

  Quote: ... AKT (9272, 1:1000 dilution, RRID: AB_329827, Cell Signaling Technology), phospho-AKT (Ser473 ...
• Senescent cells and macrophages cooperate through a multi-kinase signaling network to promote intestinal transformation in Drosophila

  Ishwaree Datta, Erdem Bangi, bioRxiv - Cancer Biology 2023

  Quote: ... mouse anti-phospho Akt (1:1000, Cell Signaling Technology #4054), mouse anti-Histone 2A gamma variant ...
• Senescent cells and macrophages cooperate through a multi-kinase signaling network to promote intestinal transformation in Drosophila

  Ishwaree Datta, Erdem Bangi, bioRxiv - Cancer Biology 2023

  Quote: ... mouse anti-phospho JNK (1:50, Cell Signaling Technology #9255), mouse anti-phospho Akt (1:1000 ...
• CAF variants control the tumor-immune microenvironment and predict skin cancer malignancy

  Agnes Forsthuber, et al., bioRxiv - Cancer Biology 2023

  Quote: ... FAP (1:200, rabbit monoclonal [D3V8A], Cell signaling #13801, RRID:AB_2798316), TAGLN (1:12.000 ...
• Bcl-xL is translocated to the nucleus via CtBP2 to epigenetically promote metastasis

  Tiantian Zhang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Histone H3K4me3 (1:1,000, Cell Signaling Technology, Cat# 9751, RRID:AB_2616028), and Histone 3 (1:1,000 ...
• Bcl-xL is translocated to the nucleus via CtBP2 to epigenetically promote metastasis

  Tiantian Zhang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and Histone 3 (1:1,000, Cell Signaling Technology, Cat# 5427) and then were incubated with horseradish peroxidase-conjugated secondary antibodies ...