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Citations for Cell Signaling Technology :
801 - 850 of 947 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... SA-β-gal staining was performed at pH 6.0 (Kurz et al., 2000) using a commercial kit (Cell Signaling, #9860). To compare the intensity of SA-β-gal staining ...
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bioRxiv - Cancer Biology 2021Quote: ... underwent antigen retrieval and were incubated with primary antibodies (KIT, DakoCytomation/Agilent; Ki-67, GeneTex; cleaved caspase 3, Cell Signaling). The signal was detected using biotin-conjugated secondary antibodies (Abcam) ...
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bioRxiv - Immunology 2021Quote: SA-β-gal activity was detected in IMR-90 cells using a SA-β-gal staining kit (Cell Signaling, #9860). IMR-90 cells (40,000 cells/well in a 12-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... The BrdU assay was performed with the BD Accuri C6 flow cytometer using the APC-BrdU flow kit according to the manufacturer’s instructions (Cell Signaling).
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bioRxiv - Cell Biology 2020Quote: Cryo-embedded sections were stained using the senescence associated β-galactosidase (SA-β-Gal) staining kit (Cell Signaling Technology, 9860) as per the manufacturer’s instructions with the following modifications for tissue sections ...
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bioRxiv - Cancer Biology 2021Quote: ... RAS-GTP pulldown was performed according to the manufacturers’ instructions using the Active Ras Detection Kit (Cell Signaling Technology, #8821). In brief ...
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bioRxiv - Genetics 2022Quote: ... Chromatin from crosslinked cells was fractionated by digestion with micrococcal nuclease using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling) according to the manufacturer protocol ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... lysed and active RAS levels were measured by affinity purification using an Active Ras Detection Kit (Cell Signaling Technologies, 8821S). Pulldown samples were loaded on a 4-12% Bis-Tris NuPage Protein gel (NuPage ...
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular cAMP concentrations were determined using a cyclic AMP XP Assay Kit (Cell Signaling Technology, catalog number 4339S, Danvers, MA) using 50μg total protein per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... K-□-GG remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-□-GG) Kit (Cell Signaling Technology). Crosslinking of antibodies to beads and subsequent immunopurification was performed with slight modifications as previously described62 ...
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bioRxiv - Cancer Biology 2020Quote: Senescence-associated-β-galactosidase (SA--β-gal) staining was performed using the Senescence β-Galactosidase Staining Kit (Cell Signaling, #9860) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: We subjected freshly fixed samples to β-galactosidase (β-gal) staining by using either β-gal staining kit (Cell Signaling), following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... cGMP was measured in duplicate according to manufacturer’s directions using the Cyclic GMP XP® Assay Kit (Cell Signaling #4360) after the supernatant was diluted 1:10 in 1× Cell Lysis Buffer (Cell Signaling #9803) ...
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bioRxiv - Cancer Biology 2023Quote: ... β-galactosidase activity in cells was detected using the Senescence β-galactosidase staining kit (Cell Signaling Technology, Danvers, MA, USA) at pH 6.0 ...
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bioRxiv - Cancer Biology 2023Quote: Samples for ChIP-seq experiments were prepared using the SimpleChIP Plus Enzymatic chromatin IP kit (Cell Signaling Technology, Cat. 9005) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Immunoprecipitation was performed using a PAR-CLIP kit (Axl-Bio, China) and anti-HuR antibody (Cell Signaling Technology, USA, 12582S) according to the instructions ...
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bioRxiv - Cell Biology 2022Quote: S2R+ cells expressing Flag-dwg were subjected to ChIP assays using SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ChIP-seq analysis from P35-42 mouse cortex was performed using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9003) following manufacturer’s instructions with slight modifications ...
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bioRxiv - Biochemistry 2023Quote: Kε-GG peptides were enriched using the PTMScan HS Ubiquitin/SUMO Remnant Motif (Kε-GG) Kit from Cell Signaling Technologies using the provided reagents and protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were subjected to enrichment of tyrosine phosphorylated proteins using an the PTMScan HS phopshotyrosine kit (Cell Signaling Technology, #38572) following in-solution tryptic digestion and desalting ...
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bioRxiv - Cancer Biology 2022Quote: Senescence was assessed using the Senescence β-Galactosidase Staining Kit at pH 6 following the manufacturer’s instructions (Cell Signaling Technology). For SAHF staining ...
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bioRxiv - Molecular Biology 2023Quote: ... Senescence-associated (SA) β-galactosidase activity was determined by using the Senescence SA-β-Galactosidase Staining Kit (Cell Signaling, #9860), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... DiGly peptide enrichment was performed using the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology (CST)) ...
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bioRxiv - Cancer Biology 2023Quote: Senescence-associated β-galactosidase staining was performed using a senescence β-galactosidase staining kit (Cell Signaling Technology, cat. no. 9860) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... was used as secondary for 30 min and the signal was developed using the SignalStain DAB Substrate Kit (Cell Signaling). For IF ...
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bioRxiv - Neuroscience 2024Quote: β-Gal staining was performed with a Senescence β-Galactosidase Staining Kit (Cat. number 9860; Cell Signaling Technology, Danvers, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The SA-β Gal-staining assay for culture cells was performed using the Senescence β-Galactosidase Kit (Cell Signaling Technology) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... and incubated with annexin V and propidium iodide (PI) as per the manufacturer’s instructions (Early Apoptosis Detection Kit, Cell Signaling Technology). Cells were analyzed by flow cytometry using a BD FACSCanto cell analyzer (BD Biosciences) ...
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bioRxiv - Physiology 2022Quote: The acetyl peptide enrichment was performed on 3 mg of digested peptides using PTMscan Acetyl-Lysine Motif kit (Cell Signaling #13416). The peptides were mixed with 100 µL of 10x IP buffer (500 mM MOPS ...
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bioRxiv - Molecular Biology 2021Quote: ... SA-β-gal activity staining was performed at pH 6.0 (Kurz et al., 2000) using a commercial kit (Cell Signaling, #9860). To compare the intensity of SA-β-gal staining ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed on ice at 1000 cells/µL lysis buffer according to the manufacturer’s protocol of the active RAS detection kit (Cat.#8821; Cell Signaling Technology). Total protein concentrations of samples were measured using a BCA protein-assay kit (Cat.#23225 ...
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bioRxiv - Cell Biology 2021Quote: Chromatin immunoprecipitation assay was performed using SimpleChIP® Enzymatic Chromatin IP Kit - Magnetic Beads (Cell Signaling Technology, Inc., Danvers, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... DiGly remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-ɛ-GG) Kit (Cell Signaling Technology (CST)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins samples were processed in SDS free lysis buffer for the assessment of Ras-GTP level using Active Ras Detection Kit (Cell Signaling) by following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Senescence-Associated β-galactosidase activity was assessed using a SA-β-galactosidase staining kit according to the manufacturer’s instructions (Cell Signaling). Positive cells were counted manually out of more than 100 cells total.
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bioRxiv - Cancer Biology 2021Quote: ... Detection of the HRP/ peroxidase enzyme was performed with SignalStain® DAB Substrate Kit from Cell Signaling (cat. no. 8059; www.cellsignal.com), per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: CUT&RUN experiments were conducted using 200,000 PS or PSP cells using the CUT&RUN assay kit (Cell Signaling Technology, 86652). Briefly ...
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bioRxiv - Genomics 2022Quote: ... Cleaved Caspase1 and cleaved Gasdermin D antibodies were purchased as a part of Pyroptosis Antibody Sampler Kit (#43811T, Cell Signaling Technology). After overnight primary incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... Ubiquitinated peptides were enriched by immunoaffinity purification using the PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology, 5562) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... ChIP was performed using an amount of BEL-ChIP nuclear extract corresponding to 3.4 μg of input genomic DNA in 250 μL of ChIP Buffer + 1X PIC (SimpleChIP Kit #9003, Cell Signaling Technology). Antibody was added according to the vendor’s specifications and incubated overnight with end-over-end mixing at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... All ChIP was performed using the SimpleChIP Enzymatic Chromatin IP kit with magnetic beads according to the manufacturer’s instructions (Cell Signaling Technologies). DNA levels were quantified by qPCR amplification using Power SYBR Green Mastermix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: GTP-bound GTPase pull-down assays were performed using the Active Rac1/CDC42 Detection Kit (Cell Signaling Technology, Cat. #8815, 8819). After transfection or verteporfin treatment ...
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bioRxiv - Neuroscience 2020Quote: ... or WT N2aC plated in chamber slides using the Annexin V-FITC Early Apoptosis Detection Kit (Cell Signaling Technology, Danvers, MA). As a positive control for apoptosis and necrosis ...
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bioRxiv - Developmental Biology 2023Quote: ChIP-qPCR and ChIP-seq samples were prepared following the instructions of the SampleChIPR Enzymatic Chromatin IP Kit (Cell Signaling, #9005). Briefly ...
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bioRxiv - Neuroscience 2023Quote: Senescence-associated β galactosidase assay was performed according to the manufacturer’s protocol and as described above (Cell Signaling Technology, Kit #9860). 5,000 cells were plated on each well of a 96-well dish ...
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bioRxiv - Genomics 2023Quote: CUT&RUN experiments were conducted using the CUT&RUN (Cleavage Under Targets & Release Using Nuclease) assay kit (Cell Signaling Technology, #86652) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: A glutathione assay was developed for in-chip measurements by adapting the Cellular Glutathione Detection Assay Kit (Cell Signaling Technology #13859). The monochlorobimane reagent was prepared by reconstitution in DMSO per manufacturer’s directions ...
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bioRxiv - Molecular Biology 2023Quote: The chromatin immunoprecipitation (ChIP) assay was performed with SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell signaling technology, #9005) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Chromatin was sheared using a Covaris Sonicator for 20 minutes in sonication buffer (SimpleChIP Plus Sonication Kit, Cell Signaling Technology, #57976) to obtain fragments in the range of 200-500 bp ...
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bioRxiv - Developmental Biology 2022Quote: ... The soft palatal tissue from E13.5 embryos was collected and fixed according to the manufacturer’s instructions based on Cut and Run assay kit (Cell Signaling, 86652). Ten μl of IgG isotype control (Cell Signaling ...