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Citations for Cell Signaling Technology :
801 - 850 of 10000+ citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Hep3B cells were stimulated with human IL-6 (Cell Signaling Technology) at a concentration of 20ng/ml for 24 hours.
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated at 4 °C with primary antibodies against rabbit phospho-ERK (1:200 D13.14.4E Cell Signaling®) and mouse NeuN (1:500 MAB377 MERCK® ...
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bioRxiv - Biochemistry 2020Quote: ... supernatant was mixing end over end for 1 hour at 4°C in mTOR IgG (2983, Cell Signaling Technology) or control IgG ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at 4 °C overnight with the following antibodies: anti-PKR (1:1000; Cell Signaling cat. No. 3072), anti-p-PKR (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by incubation with the primary antibodies over night at 4°C: ATG5 (1:1000; Cell Signaling Technology, 2630s), ATG7 (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated overnight at 4□°C with primary antibodies to galectin1 (1:100, Cell Signaling, #12936S) diluted in 5% NDS-1X PBS ...
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bioRxiv - Cell Biology 2021Quote: ... blots were probed with primary antibodies at 4°C overnight: rabbit anti-phospho-eIF2αS51 (Cell Signaling, #9721, 1:1000), rabbit anti-eIF2α(Cell Signaling ...
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bioRxiv - Pathology 2022Quote: ... Sections were incubated with rabbit monoclonal antibodies against olfactomedin-4 [D6Y5A] (Cell Signaling Technology Cat# 39141, RRID:AB_2650511, 1:400), Transferrin Receptor [EPR20584] (Abcam Cat# ab214039 ...
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bioRxiv - Neuroscience 2021Quote: ... and subsequently incubated with primary antibodies overnight at 4°C (anti-PKD1 (1:1000, Cell Signaling Technology #2052, RRID:AB_2172539), anti-pS916PKD (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were applied for 24 h at 4°C: anti-LC3b (1:100; Cell Signaling Technology), anti-p62 (1:400 ...
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bioRxiv - Cancer Biology 2022Quote: ... the lysates were incubated at 4°C for 5 h with antibodies for BRD4 (Cell Signaling Technology; 1:100). Antibody/antigen complexes were recovered using Dynabeads Protein A/G (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The blots were incubated with the following primary antibodies overnight at 4 °C: SOX2 (1:1000, D9B8N, Cell Signaling), OCT4 (1:1000 ...
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bioRxiv - Genetics 2022Quote: ... Protein lysates were incubated overnight at 4°C with mouse α-Myc antibody (1:400, [Cell Signaling Technologies, #2276]) on a rotisserie tube rotator ...
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bioRxiv - Plant Biology 2022Quote: ... AtRsmD-4×Myc fusion proteins were detected using a mouse anti-Myc monoclonal antibody (1:10,000; Cell Signaling Technology).
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bioRxiv - Neuroscience 2024Quote: ... they were incubated overnight at 4 °C with primary antibodies: rabbit anti-HA (1:200, Cell Signaling Technology, 3724S), chicken anti-GFP (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated overnight at 4℃ with rabbit anti-c-Fos antibody (1:1000 dilution, #2250, Cell Signaling Technology), mouse anti-OT antibody (1:1000 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated overnight at 4°C with rabbit V5-Tag (D3H8Q) antibody (Cell Signaling Technology, 13202, 1:2000) in blocking buffer with 0.2% Tween 20 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated overnight at 4°C with rabbit α-phospho-ERK 42/44 (1:500, Cell Signaling Technologies). Slides were next incubated with AlexaFluor-555 tagged donkey-α-rabbit secondary antibody (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was incubated overnight at 4 °C with primary antibodies against phospho-ERK1/2 (1:1,000; Cell Signaling), ERK1/2 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were probed overnight at 4°C with the following specific primary antibodies: GSK3β 1:1000 (Cell Signaling, #9336), pSer9-GSK3β 1:500 (BD Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... and probed overnight at 4°C with primary antibodies: anti-cMyc (Cell Signaling technology, catalog no. 2276, 1:1000), anti-HA (Cell Signaling technology ...
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bioRxiv - Cell Biology 2024Quote: ... IPs were incubated rotating overnight at 4°C with either IgG Isotype control (1:300, Cell Signaling Technology, 2729) or anti-ERH (1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were incubated overnight at 4°C with primary antibodies: anti-phosphotyrosine antibody (1:10,000; 8959; Cell Signaling), anti-GLUT4 antibody (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were incubated overnight at 4°C in primary antibody: F4/80(1:150, Cell Signaling, Cat. No.:70076), slides were incubated in secondary antibody for 1hr at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated with primary antibody rabbit-anti-FES (85704, Cell Signaling Technologies, 1:1000, o/n, 4 °C) in 5% (w/v ...
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bioRxiv - Genomics 2022Quote: ... H3K9me3 immunoprecipitation was performed on 3 million cells with 8 µL of H3K9me3 antibody (Cell Signaling, 13969S Lot #3). Samples were sequenced using a NovaSeq SP kit (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto, CA, USA] and anti-Gapdh [1:5000; Cell Signaling, Davers, MA, USA; 14C10]).
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag ...
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bioRxiv - Microbiology 2022Quote: ... the blot was incubated at 4 °C with primary antibodies (rabbit anti-Flag, Cell Signaling, Cat #: 14793, 1:3,000 dilution) (rabbit anti-Calnexin, Cell Signaling, Cat #: 4691, 1:2,000 dilution) (rabbit anti-COX4 ...
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bioRxiv - Cancer Biology 2021Quote: ... and sectioned tissues were stained with hematoxylin and eosin or with anti-cleaved caspase 3 (Cell Signaling Technology, #4850, 1:200) and anti-RSK3 antibodies (Novus Biologicals ...
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bioRxiv - Genetics 2021Quote: ... The following antibodies were used in this study: rabbit anti-cleaved caspase 3 D175 at 1:100 (Cell Signaling Technology #9661); Alexa 647 goat anti-HRP at 1:500 (Jackson ImmunoResearch Laboratories Inc ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed (3 × 10 mins) in TBST before and after incubating with a 1:10,000 anti-mouse secondary antibody (Cell Signaling, 7076S) overnight ...
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bioRxiv - Genetics 2021Quote: ... The blots were washed (3 × 10 mins) in TBST before and after incubating with a 1:10,000 anti-rabbit secondary antibody (Cell Signaling, 7074S). The blots were washed overnight before being incubated with a 1:5000 dilution of anti-HSP70-HRP (need catalog number ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed (3 × 10 mins) in TBST before and after incubating with either a 1:10,000 anti-mouse secondary antibody (Cell Signaling, 7076S) or 1:10,000 anti-rabbit secondary antibody (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies and dilutions (in TBST for primary or 3% BSA in TBST for secondary) were used for immunofluorescence: E-cadherin (#14472, monoclonal mouse, 1:100, Cell Signaling), mouse-IgG Alexa Fluor 594-conjugated secondary (#8890 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 × 10 min with TBST and incubated for 1 h with the appropriate secondary antibody (Cell Signaling Technologies) diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were incubated with a polyclonal cleaved caspase 3 primary antibody (rabbit, 1:100; 9661, Cell Signaling, Frankfurt am Main, Germany) for 60 min at room temperature followed by incubation with a monoclonal secondary horseradish peroxidase (HRP)-coupled horse-anti-rabbit antibody with AEC+ as chromogen ...
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bioRxiv - Cancer Biology 2020Quote: Immunohistochemistry (IHC) was conducted on formalin fixed paraffin embedded tissues using cleaved caspase 3 antibody (1:300, Cell Signaling, Cat# 9661), EnVision+ Dual Link HRP secondary (Agilent ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... MCF7 cell lysate as prepared above was diluted to 0.8 mg/mL total protein in 1:3 lysis buffer:dilution buffer with 0.5 nM rabbit anti-BRD4 antibody (Cell Signaling Technology; E2A7X) and 1 nM CoraFluor-1-labeled anti-rabbit nano secondary ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were blocked with 3% BSA and incubated with primary antibodies against pERK1/2 (1:500, Cell Signaling Technology, 4370) or cleaved Caspase-3 (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... and 8 μg of total protein subjected to immunoblotting against cleaved caspase-3 and −7 (1:200 dilution; RRID: AB_2341188 and RRID: AB_11178377; cat. no. 9661S and 8438S, Cell Signaling, Danvers, MA) as well as phosphorylated (1:500 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used in this study were 1:2,500-diluted mouse anti-β-actin (Wako, #017-24551) and 1:1,000-diluted rabbit anti-cleaved caspase-3 (Cell Signaling, #9661). The membrane was washed three times with PBS for 10 minutes and incubated with secondary antibodies at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... The fixed and permeabilized cells were then labelled with antibodies against cleaved caspase 3 (Cell Signaling Technology; 9761; 1:500 dilution) and counterstained with DAPI ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was then washed 3 times in 1x TBST and 1:3000 anti-rabbit secondary antibody (Cell Signaling Technology #7074) in 5% milk TBST was added ...
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bioRxiv - Immunology 2022Quote: ... αvinculin (Cell Signaling 4650T, lot 4), αlaminB1 (Cell Signaling 13435 ...
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bioRxiv - Immunology 2022Quote: ... caspase-4 (#4450; Cell Signaling Technology), caspase-8 (#9746 ...
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bioRxiv - Pathology 2023Quote: ... total Histone 4 (Cell Signaling #2592), a-SMA (Dako M085101 ...
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bioRxiv - Neuroscience 2021Quote: Brains were homogenized using Qiagen Tissue-Lyser II (28 Hz, 3 min, 3 times) in lysis buffer (Cell Signaling #9803) containing protease inhibitor cocktail (Roche #4693159001 ...
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bioRxiv - Immunology 2023Quote: ... or fixed for caspase 3 analysis using Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor® 488 Conjugate) (Cell signaling, 9669S). Apoptosis and pyroptosis was assessed by adding Annexin V-FITC (Biolegend ...
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bioRxiv - Developmental Biology 2023Quote: ... or normal rabbit IgG (6 μg per ChIP, Cell Signaling Tech., 2729P) as a negative control was incubated with digested chromatin overnight with rotation at 4 °C and then incubated with 30 μl Protein G magnetic beads for 2 h at 4 °C with rotation ...