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Citations for Cell Signaling Technology :
7851 - 7900 of 10000+ citations for 6 METHYL 4 4 NITRO PHENYL 2 OXO 1 2 3 4 TETRAHYDRO PYRIMIDINE 5 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... WB 1:1000), rabbit anti-TYRO3 (5585, WB 1:1000), rabbit anti-phospho-AXL (Tyr702) (5724, WB 1:1000) all from Cell Signaling Technology; mouse anti-α-tubulin (T5168 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein separation was achieved via SDS-PAGE and proteins were transferred to nitrocellulose for immunoblotting with the following primary antibody dilutions: rabbit monoclonal anti–Caveolin-1 (1:1000; Cell Signaling #3267), mouse monoclonal anti–Actin (1:1000 ...
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bioRxiv - Biophysics 2021Quote: ... and stained with 1:50 RBD-sfGFP expression medium (prepared as previously described and 1:250 anti-myc Alexa 647 (clone 9B11, Cell Signaling Technology) in PBS-BSA ...
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bioRxiv - Bioengineering 2020Quote: ... and blocked with 1% BSA for 1 hour prior to incubation with primary antibodies raised against OCT4 (Cell Signaling, Cat. # 28901:800), NANOG (Cell Signaling ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained with APC-conjugated CD45 (1:25 dilution; Miltenyi) and Alexa Fluor 488-conjugated pan-keratin (1:25 dilution, Cell Signaling Technology) antibodies for 10 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mCherry (AB0081; 1:300; SICGEN) for neuronal morphology and anti-phospho-Iϰβ-α (14D4 rabbit monoclonal antibody; 1:500; Cell Signaling) for labeling the AIS ...
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bioRxiv - Zoology 2021Quote: ... followed by sequential incubations at 4°C in one of the primary antibodies (1:1000 in blocking buffer) and an anti-rabbit HRP-conjugated secondary antibody (1:2000 in blocking buffer (Cell Signaling Technology). Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... 1:1,000, 9251), anti-c-jun (60A8, 1:1,000, 9165), anti-phospho-c-jun (S63, 1:1,000, 9261) (Cell Signaling, Beverly, MA, USA); anti-BECN1/Beclin-1 (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBS-T buffer and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit antibodies (1:5000; Cell Signaling Technology). The membranes were washed three times in TBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... from DSHB; anti-FoxO3 (1/1000; cat. no. 2497) and anti-phospho-Hsp27(Ser82) (1/1000; cat. no.2406) from Cell Signaling Technology; anti-HA (1/1000 ...
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bioRxiv - Cell Biology 2022Quote: ... and then subjected to western blot analysis using anti-Prdx5 (1:500, Ab Frontier) and anti-hnRNPK (1:500, Cell Signaling Technology) antibodies.
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bioRxiv - Cancer Biology 2022Quote: The following antibodies were used for immunoblotting (IB) at 1:1,000 unless otherwise stated: Anti-AXL (8661S, Cell Signaling, IHC 1:500), anti-phospho AXL y702 (5724 ...
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bioRxiv - Cancer Biology 2019Quote: ... and incubated for 1 hour at 25°C with a solution containing 1/400 goat α-rabbit-Alexa-488 (Cell Signaling 4412S) and 1 μM Hoechst 34580 to detect nuclear material (H21486 Molecular Probes. ...
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bioRxiv - Cancer Biology 2020Quote: ... as manufacturer’s recommended protocol following overnight incubation with anti-PAX8 mouse antibody 1:250 (Novus: NBP2-29903) and anti-SOX17 rabbit antibody 1:250 (Cell Signaling: 81778S) at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL of each substrate solution was added to 1 mL of PBS or RIPA lysis buffer (Cell Signaling Technology, Danvers, MA) for NLuc experiments ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were further incubated with α-Syn rabbit polyclonal antibody 1:500 (US biological) followed by goat anti-rabbit secondary at 1:1000 (Cell signaling) after which treated with TMB substrate ...
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bioRxiv - Cancer Biology 2020Quote: ... DyLight 800 conjugate (1:15000, #5257) and anti-rabbit IgG(H+L) DyLight 680 conjugate (1:15000, #5366) from Cell Signaling Technologies. The images were captured using Odyssey CLx Imager (LICOR Biosciences).
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bioRxiv - Cell Biology 2019Quote: ... 5% BSA (AmericanBio) in TBST (TBS with Tween 20) for 1 h at RT and incubated with AKT (1:1000, rabbit, Cell Signaling 9272), pAKT (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cat no.: 4370; Dilution 1:1000) and total p44/42 MAPK (Cat no.: 4695; Dilution 1:1000) were obtained from Cell Signaling Technology. Horseradish peroxidase (HRP)-conjugated anti-rabbit (Cat no. ...
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bioRxiv - Physiology 2021Quote: ... A mouse primary anti-CaSR monoclonal antibody (1:2000, Gentex, Zeeland, MI) or a mouse primary anti-Myc (9B11) monoclonal antibody (1:1000, Cell Signaling Technology) was applied followed by a secondary horseradish peroxidase-coupled secondary antibody (1:5000 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked using Odyssey blocking buffer and incubated in primary antibodies overnight (αTubulin, 1:1000, Cell Signaling; SIRT3, 1:1000, Cell Signaling; MCAD 1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... rabbit anti-Stat3K685ac (2523S, 1:2000 dilution), and anti-rabbit IgG HRP conjugate (7074P2, 1:10000 dilution) were from Cell Signaling Technology. Anti-tubulin hFAB Rhodamine (12004166 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the following antibodies were used anti-Bassoon (1:400, SAP7F407; mouse monoclonal; Enzo Life) and anti α-synuclein (1:500, D37A6; rabbit polyclonal; Cell Signaling). Species-specific secondary antibodies coupled to Alexa 488 and 350 (1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5% Tween-20) and incubated 1 hour at room temperature with 1/2500 of primary antibody anti-pERK (Cat.#4377; Cell Signaling Technology) diluted saline buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Depletion of ATGL16L1 and LC3B was verified on ALI 7 days by immunoblot analysis using antibodies to rabbit anti-ATG16L1 (1:1000 vol;vol ATG16L1 1:1000 vol;vol (Cell Signaling D6D5) or rabbit anti-LC3B (1:500 vol;vol Sigma-Aldrich L7543) ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 1 h incubation with the HRP-conjugated secondary antibody (Cell Signaling 7076S, 1:10,000 in 2.5% milk in TBS-T). After three washes in TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were blocked with 1% BSA plus 10% goat serum for 1 hr and stained with the anti-phospho-paxillin (Tyr118) antibody (Cell Signaling Technology) at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... and a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (1:1000 in blocking buffer for 1 hour, Cell Signaling Technology 7074S) with the peroxidase substrate KPL TrueBlue (30 minutes ...
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bioRxiv - Systems Biology 2022Quote: ... Membranes were probed with p-E2F1 (1:1,000 dilution; cat. no. MABE1782; MilliporeSigma) or E2F1 (1:1,000 dilution; cat. no. 3742S; Cell Signaling Technology, Beverly, MA) and ROCK2 (1:1,000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... gels were transferred to Nitrocelllose membranes and probed with anti-CFTR (ab596 from Cystic Fibrosis Foundation, 1:1750) or anti-Nav 1.5 (D9J7S, Cell Signaling, 1:5000) overnight in 5% milk in TBST ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibody solution contained goat anti-mouse antibodies FITCI at 1:1000 dilution and goat anti-rabbit dilution TRITCI at 1:1000 dilution (from Cell Signaling Technologies) and incubated in humidity chambers at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h at room temperature and blotted with an antibody against phosphorylated upstream frameshift protein 1 (phospho-UPF1) (pUPF1; 1:1000, Cell Signaling Technologies) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 488-conjugated rabbit monoclonal anti-phospho-histone H3 (pH3) (1:200 for IF and 1:50 for flow cytometry; D2C8; Cell Signaling Technology); and fluorescence (Alexa Fluor 488 or Alexa Fluor 594 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were detected secondaries antibodies diluted in PBS with 1% goat serum: polyclonal Alexafluor 594-conjugated anti-rabbit IgG (1:1000, Cell Signaling, 8889), and polyclonal Alexafluor 488-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... p-S6S235/236 (#2211; 1:500) and p-4EBP1T37/46 (#2855; 1:1000) antibodies were obtained from Cell Signaling (Danvers, MA, USA). Cre antibody (NB100-56135 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:3000), anti-p21 Waf1/Cip1 (clone 12D1, #2947, 1:3000), anti-Vinculin (clone E1E9V, #13901, 1:5000) were from Cell Signaling Technology. Goat anti-rabbit antibody linked to horseradish peroxidase (HRP ...
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bioRxiv - Systems Biology 2023Quote: ... Anti-caspase-8 antibody (4790, dilution 1:1000) and anti-cleaved caspase-8 antibody (8592, dilution 1:500) were purchased from Cell Signaling Technology. Anti-p-RIP3 antibody (ab222320 ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... The sections were incubated with primary antibody (pS6 Cell Signaling 2215, 1:1000; GFAP Cell Signaling #3670, 1:500; NeuN Cell Signaling D4G40 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed in TTBS/1% skim milk, incubated with secondary antibodies (1:5000 anti-mouse IgG, HRP-linked antibody (Cell Signaling #7076S)) ...
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bioRxiv - Systems Biology 2023Quote: ... rinsed in wash buffer and incubated for one hour in the primary antibodies (PAK4 Santa Cruz Biotechnology sc-390507 1:75 primary dilution, TGFBI Abcam ab170874 1:300 primary dilution, DDR1 Cell Signaling #5583 primary dilution 1:2000 ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used in this study: rabbit anti-cleaved Dcp-1 (Asp215) (1:250, Cell Signaling, Danvers, MA, USA), rabbit anti-peroxidasin (Pxn ...
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bioRxiv - Genomics 2023Quote: ... The membrane was washed five times with TBST and then incubated for 1 h with secondary goat-anti-rabbit-HRP conjugate (1:4000, Cell Signaling Technology) in TBST with 5% non-fat powdered milk ...
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bioRxiv - Immunology 2023Quote: ... blocked with 5% fat-free milk in TBST and probed with a 1:1000 dilution of an antibody that recognizes the doubly-phosphorylated TGY motif of PMK-1 (Cell Signaling Technology) or a monoclonal anti-tubulin antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were incubated with appropriate primary antibodies (phospho-ATM, 1:1000, Cell Signaling Technology, #13050; E-Cadherin, 1:1000, Cell Signaling Technology, #3195 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-phospho-IRF3 (4947S, 1:1,000 dilution) and anti-phospho-TBK1 (5483T, 1:2,000 dilution) were purchased from Cell Signaling Technology (Massachusetts, USA). Anti-phospho-p65 (A19653 ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were combined with QD-fibril complexes in a total volume of 200 μL with 1% milk and 1:1000 mouse anti-Myc antibody (Cell Signaling, 2276S) and allowed to incubate with end-over-end mixing at room temperature for approximately 3 h ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated overnight at 4°C with rotation in the respective antibody (IgG [1:100; Jackson ImmunoResearch, 111-005-003, RRID: AB_2337913], H3K27ac [1:66; Cell Signaling Technology, 4353S ...
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bioRxiv - Physiology 2023Quote: ... 1:1000 dilution), antianti-p-MOB1 (#8699, 1:1000 dilution), and anti-MOB1 (#13730, 1:1000 dilution) antibodies were purchased from Cell Signaling Technology. Anti-phospho-Mst1 (AP0906 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit polyclonal CaMKII (1:2000, 3362), rabbit monoclonal anti-phopho-GluA1Ser831 (1:2000, 75574) and rabbit monoclonal GluA1 (1:2000, 13185) purchased from Cell Signaling Technology, Inc. ...
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bioRxiv - Pathology 2023Quote: ... 0.01%Tween 20) for 1 hour at room temperature and was followed by incubation with anti-EZH2 (1:1000, 5246S, Cell Signaling Technology) antibody ...