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• Actomyosin-II Proactively Shields Axons of the Central Nervous System from Mild Mechanical Stress

  Xiaorong Pan, et al., bioRxiv - Neuroscience 2023

  Quote: ... Calyculin A (#9902S, Cell Signaling); BAPTA-AM (#T6245 ...
• Unraveling the link between Neuropathy Target Esterase NTE/SWS, lysosomal storage diseases, inflammation, and abnormal fatty acid metabolism

  Mariana I. Tsap, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-Cas3 (#9662, 1:1000) from Cell Signaling; and rabbit anti-NrxIV (1:1000 from Christian Klämbt) ...
• Neurotrophin-3 from the dentate gyrus supports postsynaptic sites of mossy fiber-CA3 synapses and contextual memory

  Ji-Wei Tan, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-calbindin (1:400; Cell Signaling Technology #13176S), goat anti-doublecortin (1:200 ...
• Intranasal CRMP2-Ubc9 Inhibitor Regulates Na_V1.7 to Alleviate Trigeminal Neuropathic Pain

  Santiago I. Loya-Lopez, et al., bioRxiv - Neuroscience 2023

  Quote: ... Cells were then incubated overnight with anti-NeuN antibody (1/500; Cat #: D3S3I, Cell Signaling, RRID:AB_2630395) in PBS with 3% bovine serum albumin at 4°C ...
• Orthogonal analysis of mitochondrial function in Parkinson’s disease patients

  Sander Barnhoorn, et al., bioRxiv - Neuroscience 2023

  Quote: ... Mitochondria were stained with Mitotracker Green FM (100 nm, Cell Signaling, 9074) and active mitochondria with TMRM (100 nm ...
• KMT2C knockout generates ASD-like behaviors in mice

  Bastian Brauer, et al., bioRxiv - Molecular Biology 2023

  Quote: ... rinsed and developed using chemiluminescence (Cell Signaling Technology, USA). Primary antibodies used ...
• Catalpol inhibits HHcy-induced EndMT in endothelial cells by modulating ROS/NF-κB signaling

  Chengyan Wu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... p-p65 protein antibodies were purchased from Cell Signaling Technology and p65 protein antibodies were purchased from Wan Lei Biology ...
• Chronic chemogenetic activation of hippocampal progenitors enhances adult neurogenesis and modulates anxiety-like behavior and fear extinction learning

  Megha Maheshwari, et al., bioRxiv - Neuroscience 2023

  Quote: ... immature neurons immunopositive for doublecortin (DCX; rabbit anti-DCX; 1:250; Cell Signaling, USA) and adult hippocampal progenitors labeled with the nestin-GFP transgene (mouse anti-GFP ...
• Chronic chemogenetic activation of hippocampal progenitors enhances adult neurogenesis and modulates anxiety-like behavior and fear extinction learning

  Megha Maheshwari, et al., bioRxiv - Neuroscience 2023

  Quote: ... and rabbit anti-DCX (1:250; Cell Signaling). Sections were then incubated with a secondary antibody cocktail ...
• Dendritic cell-mediated responses to secreted Cryptosporidium effectors are required for parasite-specific CD8⁺ T cell responses

  Breanne E. Haskins, et al., bioRxiv - Immunology 2023

  Quote: ... TCF1 AF488 (clone C63D9) from Cell Signaling. Endogenous and OT-I responses were measured by H2-Kb:SIINFEKL tetramer conjugated to PE or APC (NIH Tetramer Core ...
• Accurate determination of house dust mite sensitization in asthma and allergic rhinitis through cytometric detection of Der p 1 and Der p 2 binding on Basophils (CytoBas)

  Lin Hsin, et al., bioRxiv - Immunology 2023

  Quote: ... followed by a secondary goat-anti-mouse HRP antibody (clone NA9310 N; Cell signaling Technology, Danvers, MA, USA) and incubation with the Luminata forte western HRP substrate (Merck Millipore ...
• An unconventional mechanism of IL-1β secretion that requires Type I IFN in lupus monocytes

  Simone Caielli, et al., bioRxiv - Immunology 2023

  Quote: ... anti-MDA5 (Cell Signaling #5321S), anti-DHX33 (Abcam #ab182006) ...
• An unconventional mechanism of IL-1β secretion that requires Type I IFN in lupus monocytes

  Simone Caielli, et al., bioRxiv - Immunology 2023

  Quote: ... Rabbit anti-AIM2 (D5X7K; Cell Signaling) and Rabbit anti-MxA (Abcam ...
• An unconventional mechanism of IL-1β secretion that requires Type I IFN in lupus monocytes

  Simone Caielli, et al., bioRxiv - Immunology 2023

  Quote: ... anti-Caspase 1 (Cell Signaling #24232S), anti-polγ (Abcam #ab128899) ...
• A host AAA-ATPase exhibits bacteriolytic activity for clearance of microbial infection

  Sourav Ghosh, et al., bioRxiv - Microbiology 2023

  Quote: ... Phosphothreonine (Cell Signaling, Technology 9381S), K63-Ub linkage (Enzo ...
• Inflammasomes primarily restrict cytosolic Salmonella replication within human macrophages

  Marisa S. Egan, et al., bioRxiv - Microbiology 2023

  Quote: ... and HRP-conjugated secondary antibodies anti-mouse IgG (F00011; Cell Signaling) and anti-rabbit IgG (7074S ...
• Inflammasomes primarily restrict cytosolic Salmonella replication within human macrophages

  Marisa S. Egan, et al., bioRxiv - Microbiology 2023

  Quote: ... Primary antibodies specific for caspase-4 (4450S; Cell Signaling) and β-actin (4967L ...
• Mammalian cells-based platforms for the generation of SARS-CoV-2 virus-like particles

  Ghada Elfayres, et al., bioRxiv - Microbiology 2023

  Quote: ... HRP-conjugated secondary anti-mouse and anti-rabbit IgG (Cell Signaling Technology) were diluted at 1:10,000 ...
• Recombinant Rotavirus Expressing the Glycosylated S1 Protein of SARS-CoV-2

  Asha A. Philip, Sannoong Hu, John T. Patton, bioRxiv - Microbiology 2023

  Quote: ... or rabbit monoclonal β-actin antibody (D6A8, Cell Signaling Technology, 1:1000). In some cases ...
• Recombinant Rotavirus Expressing the Glycosylated S1 Protein of SARS-CoV-2

  Asha A. Philip, Sannoong Hu, John T. Patton, bioRxiv - Microbiology 2023

  Quote: ... or goat anti-rabbit IgG (Cell Signaling Technology). Signals were developed using Clarity Western ECL Substrate (Bio-Rad ...
• Host ZCCHC3 blocks HIV-1 infection and production by a dual mechanism

  Binbin Yi, et al., bioRxiv - Microbiology 2023

  Quote: ... the cell lysate was subjected to SDS-PAGE and western blot analyses using anti-HA antibody (1:2,000, 2999S, Cell Signaling Technology).
• Enterovirus D68 capsid formation and stability requires acidic compartments

  Ganna Galitska, et al., bioRxiv - Microbiology 2023

  Quote: ... Bafilomycin A1 (Baf A1; Cell Signaling) at 0.1µM ...
• Gene-to-Gene Coordinated Regulation of Transcription and Alternative splicing by 3D Chromatin Remodeling upon NF-κB activation

  Paul Marie, et al., bioRxiv - Molecular Biology 2023

  Quote: ... IkBa (L35A5 Cell Signaling Technology). Membranes were washed three times in 1X TBS-Tween and incubated with HRP-conjugated secondary antibodies for 1 h before washing for three times ...
• ULK1-regulated AMP sensing by AMPK and its application for the treatment of chronic kidney disease

  Tomoki Yanagi, et al., bioRxiv - Molecular Biology 2023

  Quote: ... anti-phospho-ULK1 (pSer555) (1:1,000, #5869, Cell Signaling Technology), anti-ACC (1:1,000 ...
• Synaptopodin is required for long-term depression at Schaffer collateral-CA1 synapses

  Yanis Inglebert, et al., bioRxiv - Neuroscience 2023

  Quote: ... IP3R (1:1000, Cell Signaling, D53A5). Horseradish peroxidase (HRP)-conjugated secondary antibodies goat anti-rabbit (abcam ...
• Neurodevelopmental defects in a mouse model of O-GlcNAc transferase intellectual disability

  Florence Authier, et al., bioRxiv - Neuroscience 2023

  Quote: Brain homogenates were lysed using 10x RIPA buffer (Cell Signaling), centrifuged at 14,000 rpm for 20 min at 4 °C ...
• Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice

  Adriana C. Norris, et al., bioRxiv - Molecular Biology 2023

  Quote: ... phospho-IRS-1S612 (#3203, Cell Signaling), IRS-1 (#3407 ...
• Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice

  Adriana C. Norris, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Antibody to β-actin (#4970, Cell Signaling) was used to evaluate protein loading in each lane ...
• Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice

  Adriana C. Norris, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Akt (#9272, Cell signaling), phospho-GSK-3βS9 (#5558 ...
• Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice

  Adriana C. Norris, et al., bioRxiv - Molecular Biology 2023

  Quote: ... phospho-AMPKαT172 (#2535, Cell Signaling), AMPKα (#2532 ...
• Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice

  Adriana C. Norris, et al., bioRxiv - Molecular Biology 2023

  Quote: ... phospho-GSK-3βS9 (#5558, Cell Signaling), GSK-3β (#9315 ...
• Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice

  Adriana C. Norris, et al., bioRxiv - Molecular Biology 2023

  Quote: ... phospho-ACCS79 (#3661, Cell Signaling), ACC (#3676 ...
• Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice

  Adriana C. Norris, et al., bioRxiv - Molecular Biology 2023

  Quote: ... IRβ (#3025, Cell Signaling), phospho-IRS-1S612 (#3203 ...
• Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice

  Adriana C. Norris, et al., bioRxiv - Molecular Biology 2023

  Quote: ... AMPKα (#2532, Cell Signaling), phospho-ACCS79 (#3661 ...
• Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrow

  Tongling Huang, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Chromatin complexes were immunoprecipitated with primary antibody as below and incubated with protein G magnetic beads (Cell Signaling Technology #9006) overnight ...
• Empagliflozin reduces neointimal formation and vascular smooth muscle cell proliferation via the suppression of PDGF-related signaling

  Wei-Jan Chen, et al., bioRxiv - Molecular Biology 2023

  Quote: ... cleaved caspase-3 (Cell Signaling, Beverly, MA), p-STAT3(Tyr705)/total-STAT3 ...
• Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrow

  Tongling Huang, et al., bioRxiv - Molecular Biology 2023

  Quote: ChIP assays were performed using a ChIP assay kit (Cell Signaling Technology #9005) per the manufacturer’s instructions ...
• Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrow

  Tongling Huang, et al., bioRxiv - Molecular Biology 2023

  Quote: ... or normal rabbit IgG (1 μg/IP, Cell Signaling Technology #2729). To determine physical binding of ESRRA on the Lepin promoter ...
• Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrow

  Tongling Huang, et al., bioRxiv - Molecular Biology 2023

  Quote: ... These cells were then subjected to ChIP assays with primary antibody against ESRRA (10 μl/IP, Cell Signaling Technology #13826) or normal rabbit IgG ...
• Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrow

  Tongling Huang, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 250 nM LEPTIN receptor antagonist Allo-aca (MCE #HY-P3212) or an equal volume of vehicle IgG (Cell Signaling Technology #2729). The formation of mineralized nodules was evaluated by alizarin red S staining ...
• Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrow

  Tongling Huang, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Sections were then washed and nuclei were counterstained with DAPI (Cell Signaling Technology #4083) for 30 min at room temperature ...
• MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylation

  Galina Boldina, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Antibodies specific for the following proteins were purchased from Cell Signaling Technology: MET (mouse ...
• KDM6A facilitates Xist upregulation at the onset of X inactivation

  Josephine Lin, et al., bioRxiv - Molecular Biology 2023

  Quote: Cut&Run for KDM6A was done in wt cells using an antibody against KDM6A (Cell signaling) according to the manufacturer’s protocol (EpiCypher) ...
• Glaucoma-associated Optineurin mutations increase transmitophagy in a vertebrate optic nerve

  Yaeram Jeong, et al., bioRxiv - Neuroscience 2023

  Quote: ... Fresh 200 µM of MitoTracker® Deep Red FM (Cell Signaling Technology, #8778) dye solution was prepared by diluting a 5 mM of stock solution prepared in DMSO with filtered 0.5X MMR on the day of injection ...
• Interrogating endothelial barrier regulation by temporally resolved kinase network generation

  Ling Wei, et al., bioRxiv - Systems Biology 2023

  Quote: ... Antibody to GAPDH (Cell Signaling Technology Cat# 97166, RRID AB_2756824) was used as loading control at 1:2000 dilution ...
• Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction

  Úrzula Franco-Enzástiga, et al., bioRxiv - Neuroscience 2023

  Quote: ... Immunodetection of p-IRF3 and p-eIF4E was performed using rabbit anti-p-IRF3 (0.23 µg/mL, Cat. #4947, RRID: AB_823547, Cell Signaling Technology) and rabbit anti-p-eIF4E (1.12 µg/mL ...
• Development of MAPT S305 mutation models exhibiting elevated 4R tau expression, resulting in altered neuronal and astrocytic function

  KR Bowles, et al., bioRxiv - Neuroscience 2023

  Quote: ... TRA-1-60 (Cell Signaling Technologies 4746, 1:1000), Nanog (Cell Signaling Technologies D73G4 ...
• Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction

  Úrzula Franco-Enzástiga, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-TBK1/NAK (0.19 µg/ml, Cat. #3504, RRID: AB_2255663; Cell Signaling Technology), anti-phospho-TBK1/NAK S172 (0.141 µg/ml ...
• Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction

  Úrzula Franco-Enzástiga, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-eIF4E (0.116 µg/ml, Cat. # 9742, RRID: AB_823488; Cell Signaling Technology), anti-phospho-eIF4E S209 (0.56 µg/ml Cat ...
• Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction

  Úrzula Franco-Enzástiga, et al., bioRxiv - Neuroscience 2023

  Quote: ... they were incubated overnight at 4°C in 5% BSA/TTBS containing rabbit anti-STING (0.058 µg/ml, Cat. # 13647, RRID: AB_2732796; Cell Signaling Technology), anti-phospho-TMEM173/STING S366 (1 µg/ml ...