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Citations for Cell Signaling Technology :
6901 - 6950 of 10000+ citations for Human Breast Cancer Metastasis Suppressor 1 BRMS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: SA-β-gal was stained using the Senescence Associated β-Galactosidase Staining kit (Cell Signaling) at pH6 ...
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bioRxiv - Cancer Biology 2023Quote: ChIP assay was conducted using the CUT&RUN Assay Kit (Cell Signaling Technology, cat# 86652), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Chromatin immunoprecipitation was performed using SimpleChIP enzymatic Chromatin IP kit (Cell Signaling Technology, Cat #9003) according to the manufacturers’ suggested protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were fixed and stained following manufacturer’s recommendations (Senescence βgalactosidase Staining kit from Cell signaling). After cell staining ...
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bioRxiv - Developmental Biology 2021Quote: About 3×108 cells stored in BD Phosflow™ Perm Buffer III were washed twice with 1% PBSA buffer (PBS containing 1% BSA) and then incubated with rabbit anti-SOX1 antibody (Cell Signaling Technology, #4194) at 1:150 dilution for 1 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... blocked with 5% milk powder in TBST and probed with a 1:1000 dilution of an antibody that recognizes the doubly-phosphorylated TGY motif of PMK-1 (Cell Signaling Technology, #9211), a previously characterized total PMK-1 antibody [50] ...
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bioRxiv - Pathology 2021Quote: ... and incubated with primary antibodies at 4°C overnight at the following concentrations: β-catenin (Cell Signaling, 8480, 1:1000 in 1% BSA), p65 (Cell Signaling ...
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bioRxiv - Immunology 2020Quote: ... anti-phospho-inhibitory subunit of nuclear factor κB (NF-κB) (IκB) kinase α (IKKα) (1:1□000 dilution, C84E11, Cell Signaling Technology, USA), anti-total-IKKα (1:1□000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Sections were permeabilized with 0.5 % Triton-X for 30 min at RT and stained over night with primary antibodies (rabbit anti-IFITM1 Cell Signaling 13126 S, 1:500 or rabbit anti-IFITM2 Cell Signaling #13530S, 1:500 or rabbit anti-IFITM3 Cell Signaling #59212S ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were washed with 1% BSA-PBS and incubated (150.000 cells per sample) with pIRF3 primary antibody (1:100, Cell signaling, #29047, clone D601M) for 1 hour at 4 °C and subsequently stained with AlexaFluor 488-conjugated goat anti-rabbit secondary antibody (1:300 ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed with 1× TBST (3 × 10 min) and incubated with anti-mouse IgG horseradish-peroxidase secondary antibody conjugate (1:10000 dilution; Cell Signaling Technology, 7076S) in 1× TBST for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with incubate for 1 hour at room temperature in 1:500 goat anti-rabbit Alexa Fluor 555 (Cell Signaling Technology, 44135), 1:400 Alexa Fluor 488 Phalloidin (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... MilliporeSigma, Cat# V8012), Rabbit anti-HA antibody (1:1000, MilliporeSigma, Cat# H6908) and Mouse anti-Myc antibody (1:1000, Cell Signaling, Cat# 2276S) as primary antibody ...
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bioRxiv - Physiology 2019Quote: ... phospho-p44/42 MAPK Thr202/Tyr204 antibody (rabbit, 1:2000) and Erk1/2 antibody (rabbit, 1:2000; all from Cell Signaling Technology, USA), and a-tubulin antibody (mouse,1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were incubated for 1 hour with 1:2000 dilution of either horseradish peroxidase (HRP)-linked horse anti-mouse IgG (Cell Signaling Technologies #7076) or HRP-linked goat anti-rabbit IgG (Cell Signaling Technologies #7074 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and incubated in 5% non-fat dry milk in 1× PBS and 0.1% Tween 20 with the primary antibodies (PHC1 Cell Signaling #13505 1:1000; Rybp Cell Signaling D8J7W 1:1000; SUZ12 Cell Signaling (D39F6) 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were blocked at room temperature with 1% BSA for 1 h and incubated overnight at 4 °C with primary antibodies: rabbit Collagen I antibody (Cell Signaling Technology, 84336), mouse E-cadherin antibody (Abcam ...
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bioRxiv - Physiology 2021Quote: ... 0.4% Triton and 1% BSA in KPBS for 20 minutes and then incubated in rabbit-α-pSTAT3 (1:500; 9145 from Cell Signaling Technology) or mouse-α-Oxytocin (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Loading for monoubiquityl-Histone H2B blots was normalized to mouse anti-β-tubulin (1:1000 dilution, DSHB #E7) with anti-mouse-HRP secondary (1:1000 dilution, Cell Signaling #7076).
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total extracellular signal-regulated kinase antibody (p/total ERK 1-2 1:1000 CST4396, CST4696, Cell Signaling Technology, Danvers, MA), anti-phospho and total protein kinase B antibody (AKT 1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... using heat-induced antigen retrieval at pH 9.0 for 20 minutes, followed by primary antibody (PD-1, clone EH33; dilution 1:200) (Cell Signaling, Beverly, MA, USA) incubation for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed twice with PBS and horse-anti-mouse-IgG-conjugated to HRP was added (1:2000 in 1% milk in PBS, Cell Signaling Technology, S7076) at room temperature for 45 min ...
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bioRxiv - Immunology 2020Quote: ... membranes were incubated with primary antibodies for 1h at RT with shaking (1:2000 anti-filaggrin clone Poly19058, Biolegend; 1:2000 anti-β-actin, clone 13E5, Cell Signaling Technology). After washing (3×5 min in Tris-buffered saline containing 0.1% v/v Tween20 ...
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bioRxiv - Plant Biology 2022Quote: ... membranes were incubated overnight at 4°C with the primary antibody in 1 % milk solution in TBS (phospho-AMPK, 1:2000, Cell Signaling Technology, #2535). The next day ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were subsequently blocked with 5% milk or 5% bovine serum albumin for 1 h at room temperature and probed separately with specific primary antibodies to AKT1 (1:1,000 dilution, 75692, Cell Signaling Technology, Danvers, MA), pan-AKT (1:1,000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were washed and then blocked using 1% Goat serum in TBS for one hour at room temperature before adding primary antibodies to detect Cd45 (Cell Signaling, 1:500) and Cd11b (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-otoferlin (Developmental Studies Hybridoma Bank, HCS-1) diluted at 1:100 and rabbit anti-cleaved caspase-3 (Cell Signaling Technology, 9661) diluted at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... or 5% non-fat dry milk in TBST for 1 h then incubated overnight at 4°C in blocking buffer containing antibodies against TMEM106B (1:500, Cell Signaling Technology 93334), TDP-43 (1:1,500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% non-fat dry milk in TBST for 1 h and then incubated overnight at 4°C in blocking buffer containing the antibody against TMEM106B (1:500, Cell Signaling Technology 93334). The secondary antibody ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... the sections were subjected to antibody immunofluorescent staining (pS6 Cell Signaling 2215, 1:1000; GFAP Cell Signaling #3670, 1:500; NeuN Cell Signaling D4G40 ...
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bioRxiv - Immunology 2022Quote: ... Intracellular phospho-stainings were performed 1 h at room temperature in the dark with antibodies against phospho-STAT5 (Tyr694; D47E7 XP Rabbit mAb #4322; Cell Signaling, 1:150), phospho-S6 ribosomal protein (Ser235/236 ...
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bioRxiv - Genomics 2023Quote: ... Western blotting was performed according to standard procedures using the following antibodies and dilutions: TP53 (DO-1, 1:500, Cell Signaling #18032, mouse), CDKN1A (F-5 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then blocked with immunofluorescence (IF) block buffer (3% BSA in PBS) for 1 hour and incubated with primary antibodies: rabbit anti-γH2A.x (Cell Signaling 9718, 1:2000), rabbit anti-RPA32 (GeneTex GTX113004 1:2000) ...
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bioRxiv - Cell Biology 2023Quote: ... 2μg/mL, Ab189034, Waltham, MA 02453, USA), Klf4 (1 μg/mL, CST4038T) and β-actin (1 μg/mL, 4967S) from Cell Signaling Technology, Danvers ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were blocked with 3% BSA in PBS for 1 hour and cells were stained with VEGFR2 (Cell Signaling; C#2479; 1:200) and EEA1 (R&D Systems ...
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Specific patterns of neural activity in the hippocampus after massed or distributed spatial trainingbioRxiv - Neuroscience 2023Quote: ... After 1 h of incubation in PBST containing 1% BSA and 1% NGS sections were incubated overnight in anti-phospho-c-Fos rabbit monoclonal antibody (5348S; Cell signaling Technology, USA) diluted 1:8000 in PBST– BSA–NGS ...
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bioRxiv - Bioengineering 2023Quote: ... Staining was performed by incubating the cells overnight at 4°C with primary antibodies diluted in 1% BSA targeting α-smooth muscle actin (1:200; 48938S, Cell Signaling Technology), gamma actin (1:500 ...
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bioRxiv - Genomics 2023Quote: ... Cells were proceeded for IF as described above using anti-CD56 mouse antibodies (1:10, Coulter #PN4235479-G) and anti-TCF7L2 rabbit antibodies (1:100, Cell Signaling Technology #C48H11), revealed by Cy3-coupled anti-mouse IgGs and Cy5-coupled anti-rabbit IgGs.
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Neuroscience 2024Quote: ... and 2 h later they were labeled with mouse anti-Iba1 (1:500; Fujifilm Wako Chemicals, VA, USA) or rabbit anti-p75NTR (1:750; Cell signaling, MA, USA) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were rat anti-ATF4 (1:200, (Hunt et al., 2019)) and rabbit anti-P-eIF2α (1:500, anti-Phospho-eIF2α [Ser51], Cell Signaling Technology 9721). Secondary antibodies were goat anti-rabbit Alexa Fluor 546 (Invitrogen A11035 ...
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bioRxiv - Bioengineering 2023Quote: ... secondary antibodies were incubated for 1 hour at room temperature (Secondary antibodies: Anti-Rabbit HRP-conjugated Cell Signaling Technology (CST-#7074S 1:5000); Anti-Mouse HRP-conjugated (R&D ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 hour and then incubated with a 1:250 dilution of monoclonal rabbit F4/80 or 1:150 of rabbit Ly-6G (Cell Signaling, Danvers, MA) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti phospho-myosin light chain 2 Thr18/Ser19 (pMLC, 1:50 for IF, 1:20 for IG) was from Cell Signaling Technologies (#3674). Chicken anti-map2 (against residues 2-314 of human map2 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were incubated for 1 h at room temperature with appropriate secondary antibodies: goat anti-rabbit (1:2000, Cell Signaling Technology, Cat. 7074), goat anti-rat (1:10000 ...
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bioRxiv - Physiology 2024Quote: ... The PVDF membrane was incubated with 5% w/v non-fat dry milk or 5% BSA for 1 h at room temperature and immunoblotted with anti-HSL (phospho-serine 660) (1:1000, Cell Signaling Technology, 45804S), anti-HSL (1:1000 ...
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bioRxiv - Physiology 2024Quote: ... and /or anti-TOM20 antibody (diluted 1:1000, SantaCruz; Cat#sc177615) or/and anti-DRP1 antibody (diluted 1:50 Cell signaling; Cat#8570). After several washes with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The blots were probed with the following primary antibodies and 0.2% Tween20 with overnight incubation at 4°C: anti-p-eIF2α (Abcam; ab32157; 1:1000)(Cell Signaling; 3398s; 1:1000), anti-total-eIF2α (Cell Signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% BSA in TBS for 1 hr and then incubated overnight at 4° C with anti-pSMAD1/5/8 (1:1000, Cell Signaling Technology, 13820), anti-SMAD1 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% Triton X-100 in PBS) for 1 hour at room temperature and with primary antibodies anti-Calbindin (Cell Signaling, 2173, 1:100), anti-KDEL (Novus Biologicals ...