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Citations for Cell Signaling Technology :
6901 - 6950 of 8290 citations for Coatomer Subunit Epsilon COPE Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Blots were washed (3 × 10 mins) in TBST before and after incubating with a 1:10,000 anti-mouse secondary antibody (Cell Signaling, 7076S) overnight ...
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bioRxiv - Genetics 2021Quote: ... The blots were washed (3 × 10 mins) in TBST before and after incubating with a 1:10,000 anti-rabbit secondary antibody (Cell Signaling, 7074S). The blots were washed overnight before being incubated with a 1:5000 dilution of anti-HSP70-HRP (need catalog number ...
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bioRxiv - Genetics 2021Quote: ... The blots were incubated overnight at 4 degrees Celcius in a 1:2000 dilution of anti-AUF1 antibody (Cell Signaling D6O4F). The blots were washed (3 × 10 mins ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed (3 × 10 mins) in TBST before and after incubating with either a 1:10,000 anti-mouse secondary antibody (Cell Signaling, 7076S) or 1:10,000 anti-rabbit secondary antibody (Cell Signaling ...
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bioRxiv - Genetics 2021Quote: ... containing 5% (w/v) skimmed dried milk powder and immunoblotted using Phospho-(Ser/Thr) ATM/ATR Substrate Antibody (#2851 Cell Signaling) (dilution 1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... The bead-immobilized GTP-bound RhoA and total RhoA in the lysates were probed by immunoblotting with anti-RhoA antibody (Cell Signaling).
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bioRxiv - Molecular Biology 2021Quote: ... monoclonal antibodies followed by the by the anti-mouse IgG Alexa fluor 488 or anti-rabbit IgG Alexa fuor 555 secondary antibody (Cell Signaling). Then samples were re-fixed in 2% formaldehyde ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies used at a dilution of 1:4000 were goat anti-rabbit conjugated to Alexa Fluor 488 (4412S; Cell Signaling) and goat anti-mouse conjugated to Alexa Fluor 488 (A11029 ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were diluted with lysis buffer without inhibitors and incubated overnight at 4°C with anti-PCNA antibody (1:500, #2586, Cell signaling) under mild agitation ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were labeled overnight at 4 °C with the primary antibodies anti-Ki67 (1:200, 275R, Cell Marque) and anti-phosphorylated histone 3 (1:200, 9701, Cell Signaling) for ...
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bioRxiv - Neuroscience 2022Quote: ... in primary antibodies for 48 hours at 4°C [Rabbit anti-P-4E-BP (1:600; Cell Signaling Technology, Danvers, MA), goat anti-MAP-1A (1:360 ...
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bioRxiv - Genetics 2022Quote: ... human anticentromere (1:100; Antibodies Inc., cat# 15-234) and mouse monoclonal anti-H3K9me2/3 (1:100, Cell Signaling, cat# 5327) primary antibodies ...
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bioRxiv - Physiology 2022Quote: ... The primary antibodies used were the following: anti-cleaved Casp3 (catalog n°9661, 1/500, Cell Signaling Technology, Danvers, MA, USA), anti-Smpd3 (Merck clone 14G5.1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight with primary rabbit antibodies raised against total or active β-Catenin (Cell Signaling Technology, #8480 and #8814 respectively), and primary mouse antibodies raised against α-tubulin (Santa Cruz Biotechnology #sc-5286 ...
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bioRxiv - Neuroscience 2021Quote: ... Fixed fish were immunofluorescently labeled pERK (Phospho-pp44/42 MAP Kinase) and tERK (pp44/42 MAP Kinase) antibodies (Cell Signaling Technologies) and Alexa fluorophore conjugated secondary antibodies at 1:500 dilutions ...
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bioRxiv - Neuroscience 2020Quote: ... Chromatin solutions were incubated with 10 ug of anti-HIF1α (Cayman, Rabbit, #10006421) antibody or anti-rabbit IgG (Cell Signaling #2729) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Actin was detected in parallel with O-GlcNAc by incubation with anti-β-actin (Cell Signaling Technology, Actin Antibody 13E5, #4790) at 1:5000 with detection by goat anti-rabbit secondary at 1:10000.
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies: anti-pSTAT3 (9145) and anti-Histone H3 (9515; all from Cell Signaling). Histone H3 antibody was used as a loading control ...
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bioRxiv - Immunology 2020Quote: ... Horseradish peroxidase conjugated secondary antibodies were goat anti-rabbit IgG (Agilent Cat# P0448, RRID:AB_2617138 or Cell Signaling Technology Cat# 7074, RRID:AB_209923) or sheep anti-mouse IgG (GE Healthcare Cat# NA931 ...
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bioRxiv - Neuroscience 2021Quote: ... The dilutions of HRP-conjugated secondary antibodies were 1:2000 for goat-anti-guinea pig IgG and 1:2000 for anti-mouse IgG (Cell Signaling). Signal Fire ECL substrates (Cell Signaling ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies (Anti-3-hydroxybutyryllysine [PTM Biolabs 1201], anti-p84 [GeneTex GTX70220], anti-α-Tubulin [Sigma T5168], anti-COXIV [Cell Signaling 11967S] ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked for 1 h using 5% BSA in TBS-T (0.01% Triton-X) and incubated overnight with primary antibodies (1:500 pSTAT1, 7649 Cell Signaling; 1:500 pSTAT3, 9145 Cell Signaling; 1:500 pSTAT5 ...
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bioRxiv - Microbiology 2020Quote: ... After this anti-rabbit IgG secondary antibody (for Sap and EA1) or anti-mouse IgG secondary antibody (for BslO) conjugated with horseradish peroxide (1:10,000 - Cell Signaling Technology) was used and the blots were incubated for another 60 min ...
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bioRxiv - Genomics 2020Quote: ... The following antibodies were used for SIM and immunoblot analysis: rabbit anti-HDAC6 (clone D2E5, Cell Signaling technology, Danvers, MA, USA), mouse anti-acetylated tubulin antibody (clone 6-11B-1 ...
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bioRxiv - Immunology 2021Quote: ... anti-β-actin (3700) and the secondary antibodies used for western blot and immunofluorescence assay were purchased from Cell Signaling Technology. Anti-SYVN1 (ab170901 ...
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bioRxiv - Immunology 2021Quote: ... followed by washing and 2 hour incubation with goat-anti-rabbit-HRP antibody at 1:5000 (all from Cell Signaling Technology). Membranes were washed again and detected with SuperSignal West Pico Chemiluminescent substrate (Thermofisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... Multiple western blots were performed as described above using an anti-HA antibody (Cell signaling technology #3724, dilution 1:1 000). Quantification was done using the Fujifilm Multi Gauge software V3.0.
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bioRxiv - Bioengineering 2020Quote: ... The sections were cooled to room temperature and blocked with 20% fetal bovine serum for 45 minutes and then immunostained overnight with 1:100 dilution of primary antibody to PECAM-1 (Cell Signaling), αSMA (Abcam) ...
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bioRxiv - Bioengineering 2020Quote: ... The sections were cooled to room temperature and blocked with 20% fetal bovine serum for 45 minutes and then immunostained overnight with 1:100 dilution of primary antibody to PECAM-1 (Cell Signaling), SCF (Abcam) ...
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bioRxiv - Genetics 2020Quote: ... 4°C) and immunoprecipitation was performed overnight at 4°C using an anti-NFkBp65 antibody (clone D14E12; Cell Signaling Technology 8242) or an isotype control (rabbit IgG monoclonal antibody ...
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bioRxiv - Immunology 2020Quote: ... Staining was performed as described previously31 using a primary antibody specific for a linear epitope of the N-term portion of GFP (clone D5.1, Cell Signaling Technology) or for a conformational epitope of GFP to detect correctly folded GFP (clone 3E6 ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times with TBS-T (0.05% v/v Tween-20 in TBS) before incubation with HRP conjugated antibody (Cell Signaling Technology 7076S ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were blocked for 1 h at room temperature with 5% skim milk and then incubated overnight at 4°C with appropriate primary antibody dilution: anti-STAT1 (1:1000, Cell Signaling), anti-pSTAT1 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... which was performed as previously described (27,28) using the following antibodies: rabbit monoclonal anti-Caveolin-1 (1:100, Cell Signaling #3267); mouse monoclonal anti-Glutamine Synthetase (for Müller glia-specific marker ...
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bioRxiv - Biophysics 2021Quote: ... and phospho-myosin (rabbit phospho-myosin light chain 2 (Thr18/Ser19) antibody (dilution 1:50 in PBS 0, 5% BSA, #3674, Cell Signaling) for 2 h at RT ...
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bioRxiv - Biophysics 2021Quote: ... Both primary antibodies were coupled with an Alexa Fluor 488 goat anti-rabbit secondary antibody (dilution 1:500 in PBS 0,5% BSA, #4412, Cell Signaling Technology) for 2h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... The following HRP-linked secondary antibodies were used by dilution in PBST: anti-rabbit IgG (Cell Signaling Technology, 7074S, 1:10,000), anti-mouse IgG (Zsbio ...
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bioRxiv - Biochemistry 2020Quote: IP was performed on hepatocytes lysates using antibodies against LxRxx[S*/T*] and Rxx[S*/T*] phospho-motifs (both Cell Signaling) with Pierce Protein A/G Magnetic Beads according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and probed with the following antibodies diluted in 5% skim milk/PBS (w/v): anti-HA (1:4000, clone C29F4, Cell Signaling), anti-cMyc (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... HRP-linked anti-mouse IgG or HRP-linked anti-rat IgG were used as secondary antibodies (Cell Signaling Technology, Danvers, MA). Membranes were imaged with ChemiDoc imaging system (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Blocking buffer was manually removed and replaced with either anti-Asymmetric Di-Methyl Arginine antibody (1:500, Cell Signaling Technology 13522) or anti-Symmetric Di-Methyl Arginine antibody (1:800 Cell Signaling Technology 13222 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 × 10 min with TBST and incubated for 1 h with the appropriate secondary antibody (Cell Signaling Technologies) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The digested chromatin (500 μg) was subjected to immunoprecipitation with specific TCF7L2 antibody (Celling Signaling) or anti-Rabbit IgG (#2729 Cell signaling) as negative control overnight at 4° C with rotation and followed by ChIP-Grade protein G magnetic beads incubated for 2 hours at 4° C with rotation ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were incubated with a polyclonal cleaved caspase 3 primary antibody (rabbit, 1:100; 9661, Cell Signaling, Frankfurt am Main, Germany) for 60 min at room temperature followed by incubation with a monoclonal secondary horseradish peroxidase (HRP)-coupled horse-anti-rabbit antibody with AEC+ as chromogen ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were treated with rabbit anti-V5 antibody at a 1:2000 dilution (Clone D3H8Q, #13202S, Cell Signaling Technology, Danvers, MA) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1h with A647-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling).
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1h with A488-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling).
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1h with A647-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling). Sections were washed in PBS and incubated with Goat Fab Fragment Anti-Rabbit IgG (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1 h with A647-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling). Sections were then mounted and cover-slipped.