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Citations for Cell Signaling Technology :
6801 - 6850 of 10000+ citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Cell fixation and chromatin preparation was performed using SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling). Real-time PCR primers (site 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were built using DNA Library Prep Kit for Illumina (Cell Signaling Technology, Danvers, MA). Paired-end sequencing was performed on the Illumina NextSeq instrument at the Harvard University Bauer Core ...
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bioRxiv - Cell Biology 2022Quote: Cellular senescence was evaluated by β-galactosidase activity using β-galactosidase Senescence Kit (Cell signaling, #9860) after being cultured for designated times ...
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bioRxiv - Neuroscience 2022Quote: CUT & RUN was performed using Cell Signaling Technology CUT & RUN Assay kit (Cell Signaling Technology #86652) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Preparation of CUT&RUN libraries was done with the CUT&RUN assay kit (Cell Signaling Technology). Briefly ...
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bioRxiv - Systems Biology 2023Quote: ... the PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) kit (Cell Signaling Technology, 59322) was used following manufacturer instructions ...
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bioRxiv - Pathology 2023Quote: ... and then succinylated peptides were enriched using the PTMScan Succinyl-Lysine Motif Kit (Cell Signaling Technologies) as previously described but with slight adaptations (also see Supplemental Appendix 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... SA-β-gal assay was performed using the senescence β-galactosidase staining kit (Cell Signaling #9860). At least ten randomly selected fields from the 20X magnification of the bright-field microscope (∼500 cells ...
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bioRxiv - Genomics 2023Quote: ChIP was performed using a SimpleChIP Plus Sonication Chromatin IP Kit (Cell Signaling Technology, Danvers, MA) according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Biochemistry 2023Quote: For CHIP assays we used CUT&RUN Assay Kit #86652 (Cell Signaling Technology, Danvers, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: For CHIP assays we used CUT&RUN Assay Kit #86652 (Cell Signaling Technology, Danvers, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and GTP-bound RAS was quantified using active RAS detection kit (# 8821) from Cell Signaling Technology according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... with modifications for CUT&RUN as noted in the CUT&RUN Assay Kit (Cell Signaling, #86652S). All libraries were quantified on the Qubit 4.0 and library quality assessed using Agilent High Sensitivity DNA Tapestation (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... the chromatin was processed by the simpleChIP kit according to the manufacturer’s instructions (Cell Signaling Technology). The chromatin was precipitated using antibodies against Notch3 (M-134 Santa Cruz Biotechnology or 8G5 Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... and the SimpleChIP ChIP-seq DNA Library Prep Kit for Illumina (Cell Signaling Technology, Cat. #56795) were used with protocol modifications for CUT&RUN as recommended by Cell Signaling ...
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bioRxiv - Systems Biology 2023Quote: Chromatin-immunoprecipitation experiment was carried out using SimpleChIP Plus Enzymatic Chromatin IP kit (Cell Signaling, 9004), according to the manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2023Quote: ChIP was carried out using the Simple ChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9005) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: The senescent cells were stained by a senescence β-galactosidase staining Kit (#9860, Cell Signaling Technology), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... the PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) kit (Cell Signaling Technology, 59322) was used following manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and isotype control IgG (CST, 2729) and the CUT&RUN Assay Kit (Cell Signaling Technology, 86652). The resulting DNA fragment samples were generated into libraries using the SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed using the DNA Library Prep Kit for Illumina Systems (Cell Signaling Technology, 56795S) and Multiplex-oligos for Illumina Systems (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA binding assays were performed using the CUT&RUN Kit from Cell Signaling Technologies (CST 86652) following the manufacturer’s protocol with slight modifications ...
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bioRxiv - Cell Biology 2024Quote: ... and DNA Purification using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, #9003). Samples were sonicated using a Qsonica sonicator (Qsonica Q800R ...
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bioRxiv - Molecular Biology 2024Quote: ... and DNA purified using the SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling, #9003). Anti-AM tag (active motif ...
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bioRxiv - Microbiology 2020Quote: ... for evaluation of histopathologic changes by H&E staining or for immunohistochemical detection of reovirus antigen (1:45,000 dilution) or the cleaved (active) form of caspase-3 (Cell Signaling, 9664; 1:300 dilution). Images were captured at a magnification of ×20 and a resolution of 0.5 μm/pixel using a high-throughput Leica SCN400 slide scanner automated digital imaging system.
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bioRxiv - Molecular Biology 2020Quote: ... The following primary antibodies were diluted in 1% milk in TBST prior to usage at the indicated concentration: mouse monoclonal α-Tubulin (1:3000, Cell Signaling Technology, #3873), rabbit monoclonal p-p70 S6K (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... and subjected to immunoblotting with the antibodies for phosphorylated Erk-1/2 (Thr202/Tyr 204) or total Erk-1/2 from Cell Signaling Technology (Beverly, MA). Blots were visualized by enhanced chemiluminescence western detection system (Pierce ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were then incubated for 2 hours at room temperature (RT) in antibody binding buffer (PBSTr +1% (v/v) DMSO) before overnight incubation with 1:1000 α-pSmad2 antibody (Cell Signaling Technology #18338) and ...
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bioRxiv - Cancer Biology 2019Quote: ... monoclonal HSP27 (1:1500, catalog number 2402S), MK2 (1:1000, catalog number 3042) and p38 (1:1000, catalog number 9218S) all from Cell Signaling Technology (Danvers, MA); p-HSP27 Ser78 (1:2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1:1,000 for immunoblotting), PGK1 (68540, 1:1000 for immunoblotting), and PKM2 (3198, 1:1000 for immunoblotting) were purchased from Cell Signaling Technology (Danvers, MA). Mouse monoclonal antibodies for VEGF (C-1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sections were then incubated in rabbit polyclonal anti-αSMA or rabbit monoclonal anti-Runx2 at 4° C overnight (Abcam ab5694, 1:100; Cell Signaling Technology, 1:100). Slides were thoroughly washed the next day then incubated in goat anti-rabbit IgG Alexa Fluor 647 secondary antibody for 1 hour at room temperature (ThermoFisher A-21245 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed overnight at 4°C with Rabbit anti-KHSRP (1:1000; Novus) or Rabbit anti-GAPDH (1:2000; Cell Signaling Tech, Beverly, MA), Streptavidin-HRP (1:10000 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed with 1X TBST (3 × 5 min) and then incubated with respective secondary antibodies (Horse anti-mice 1:2000; Horse anti-rabbit 1:2000, (Cell signaling technology, MA, USA) and developed by using ECL chemiluminescent reagent (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... blocked with 5% skimmed milk powder at room temperature for 1 h, the membrane was incubated with the target primary antibody with desired dilution (anti-ACE-2, 1:1000 (Cell signaling technology, MA, USA); TMPRSS2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were probed with the following antibodies at 1/2500 or 1/5000 dilution: rabbit anti-p44/42 MAPK (ERK1/2) antibody (Cell Signaling, cat # 4695 S) to detect ERK2 ...
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bioRxiv - Cell Biology 2021Quote: ... then incubated for 1.5 h at RT with either CD36 (Novus NB400-144, 1:500 dilution) or PPARγ (Cell Signaling 2430S, 1:250 dilution) and GAPDH (Abcam ab181602 ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was washed with 1× TBS for 20 min and then incubated with anti-β-actin primary antibody (1:10000 dilution; Cell Signaling Technology, 3700S) in 1× TBST with 5% milk for 2 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-CEP135, (homemade, raised against a.a. 649-1140, rabbit anti-acetylated-α-Tubulin (1:1,000 IF, 1:500 IHC; Cell Signaling Technologies, cat. No. 5335), rabbit anti-CNTROB (1:1,000 ...
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bioRxiv - Physiology 2021Quote: ... #2217), phosphorylated S6(235/236) (1:1000, #2211), and phosphorylated S6(240/244) (1:1000, #5364) were purchased from Cell Signaling Technology (Danvers, MA). Anti-puromycin (1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... In order to positively identify the proteins, the following antibodies were used: MAN1B1 (SC-100543, 1:500) and actin (1:2000) (Cell Signaling Technology, Beverly, MA). Horse radish peroxidase (HRP)-conjugated secondary antibodies were incubated with the membrane at RT for 1 hr ...
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bioRxiv - Genetics 2019Quote: L4 worms were fed overnight on an NGM plate that had been seeded with 100 μL concentrated OP50 bacteria mixed with 1 μL of 1 mM MitoTracker Deep Red FM dye (Cell Signaling Technology, Cat #8778S).
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... Membranes were then incubated with primary antibody diluted in TBS-T buffer (1:1000 Total OXPHOS Rodent WB Antibody Cocktail [abcam Cat# ab110413] or 1:1000 HSP60 XP Rabbit mAB [Cell Signaling Technology CAT#12165]) overnight at 4°C with gentle rocking ...
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bioRxiv - Microbiology 2022Quote: ... guinea pig polyclonal NSP3 (Lot 55068, 1:2000), VP6 (Lot 53963, 1:2000) antisera or rabbit monoclonal β-actin (8457S, Cell Signaling Technology (CST), 1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-ionized calcium-binding adaptor molecule 1 (Iba-1, FUJIFILM Wako Chemicals USA, Richmond, VA) and anti-glial fibrillary acidic protein (GFAP, Cell Signaling Technology, Danvers, MA) antibodies were diluted to 1:1,000 and used to incubate tissue sections overnight at 4 °C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Membranes were incubated for 1 hour at room temperature with horseradish peroxidase-conjugated horse anti-mouse IgG solution (1:2500, Cell Signaling, Cat. No. 7074P2) diluted in 1% non-fat milk/TBST ...
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bioRxiv - Cell Biology 2024Quote: ... We also used immunofluorescence with Hepatocyte Nuclear Factor 4a (HNF4α) (1/350, PP-H1415- 00, Perseus Proteomics) to define hepatocytes in combination with phospho-histone H3 (pHH3) (9701S, Cell Signaling, diluted 1/200) or Ki67 (12202S ...
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bioRxiv - Cell Biology 2024Quote: ... followed by exposure to primary antibody (anti-Alk1 Rabbit pAb, Abcam #ab68703, 1:500; or, anti-Tubulin Rabbit Ab, Cell Signaling #2144S, 1:500) overnight in 1% BSA TBS-T at 4°C ...
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bioRxiv - Immunology 2024Quote: ... anti-phospho-AMPKα (Thr172) (40H9) (#2535, diluted at 1:1000), anti-AMPKα (#2532, diluted at 1:1000) antibodies from Cell Signaling Technology (Massachusetts, USA), anti-IRF5 (#ab21689 ...
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bioRxiv - Immunology 2023Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with primary anti–SYK (1:1000 dilution, Cell Signaling Technology, no. 2712S), anti–p-p38 (1:1000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk in TBST for 1 h and incubated with the appropriate primary antibody overnight at 4°C: anti-NANOG (D73G4) (Cell Signaling #4903; 1:1000), anti-FAK (D2R2E ...