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• Cocaine Regulates Antiretroviral Therapy CNS Access Through Pregnane-X Receptor-Mediated Drug Transporter and Metabolizing Enzyme Modulation at the Blood Brain Barrier

  Lisa B. Fridman, et al., bioRxiv - Neuroscience 2023

  Quote: Endothelial cells were lysed with 1X RIPA buffer (Cell Signaling Technology, Danvers, MA) supplemented with 1X protease/phosphatase inhibitor (Cell Signaling Technology) ...
• Translational approach to social isolation during a global pandemic: Hippocampal somatic mutation

  Bomee Lee, et al., bioRxiv - Neuroscience 2023

  Quote: ... DCX antibody (1:1000, Cell Signaling Technology, rabbit, #4604), MAP2 antibody (1:1000 ...
• Translational approach to social isolation during a global pandemic: Hippocampal somatic mutation

  Bomee Lee, et al., bioRxiv - Neuroscience 2023

  Quote: ... NeuN antibody (1:1000, Cell Signaling Technology, rabbit, #24307), Erk antibody (1:1000 ...
• Germline mitotic quiescence and programmed cell death are induced in C. elegans by exposure to pathogenic P. aeruginosa

  Daniel P. Bollen, et al., bioRxiv - Genetics 2023

  Quote: ... An α-phospho-histone H3 (Ser10) primary rabbit antibody (Cell Signaling) was used at 1:2000 dilution ...
• ATRX guards against aberrant differentiation in mesenchymal progenitor cells

  Yan Fang, et al., bioRxiv - Genetics 2023

  Quote: ... H3K27me3 (Cell Signaling Technologies, #9733), H3K27ac (Active Motif ...
• Tempered IL-2 Signals Program PD-1 Checkpoint Blockade-Responsive Stem-like Exhausted T Cells During Priming

  Vandana Kalia, et al., bioRxiv - Immunology 2023

  Quote: ... USA) except for TCF-1 (Cell signaling) and Slamf6 (BD Biosciences) ...
• ARHGAP18-ezrin functions as an autoregulatory module for RhoA in the assembly of distinct actin-based structures

  Andrew T. Lombardo, et al., bioRxiv - Cell Biology 2023

  Quote: ... used at 1:500 (western blot) and 1:50 (immunofluorescence) and rabbit anti–phospho-MLC2 raised against phospho-Thr18/Ser19 (Cell Signaling Technology; catalog no. 3674) used at 1:500 (western blot ...
• Membrane-bound Interleukin-1α mediates leukocyte adhesion during atherogenesis

  Christina Mäder, et al., bioRxiv - Immunology 2023

  Quote: ... Subcellular fractionation was performed using the Cell Signaling Kit (Cell signaling, USA) according to the manufacturer’s instructions ...
• ABI1 regulates transcriptional activity of Androgen Receptor by novel DNA and AR binding mechanism

  Baylee A. Porter, et al., bioRxiv - Cancer Biology 2023

  Quote: rabbit anti-PARP (Cell Signaling 46D11): 9532 lot# 7 ...
• Regulation potential of transcribed simple repeated sequences in developing neurons

  Tek Hong Chung, et al., bioRxiv - Genomics 2023

  Quote: ... Fixed and permeabilized cells were then blocked in 0.5% BSA and 0.2% Tween-20 in PBS (IF blocking buffer) for 30 minutes at room temperature and incubated with anti-Ngn2 (Cell Signaling Technology, cat# 13144; 1:250 dilution) and anti-MAP2 (Biolegend ...
• ABI1 regulates transcriptional activity of Androgen Receptor by novel DNA and AR binding mechanism

  Baylee A. Porter, et al., bioRxiv - Cancer Biology 2023

  Quote: rabbit anti-Stat3 (Cell Signaling 4904): 4904 lot# 7 ...
• Analysis of histone H3K4me3 modifications in bovine placenta derived from different calf-production methods

  Kanoko Hattori, et al., bioRxiv - Genomics 2023

  Quote: ChIP was performed using a SimpleChIP Plus Sonication Chromatin IP Kit (Cell Signaling Technology, Danvers, MA) according to the manufacturer’s protocol with some modifications ...
• Genomic context sensitizes regulatory elements to genetic disruption

  Raquel Ordoñez, et al., bioRxiv - Genomics 2023

  Quote: ... The CTCF antibody (Cell Signaling Technology, #2899S) was used at a dilution of 1/50 for overnight incubation at 4°C ...
• DNA methylation insulates genic regions from CTCF loops near nuclear speckles

  Shelby A. Roseman, et al., bioRxiv - Genomics 2023

  Quote: ... Concavalin A beads (Cell Signaling Technologies, 93569S) were then activated with binding buffer (20 mM HEPES-KOH pH 7.9 ...
• DNA methylation insulates genic regions from CTCF loops near nuclear speckles

  Shelby A. Roseman, et al., bioRxiv - Genomics 2023

  Quote: ... Supernatant was transferred to new tubes and antibody was added and allowed to bind overnight at 4 °C with rotation (15uL antibody per tube, Cell Signaling Technology D31H2 anti-CTCF, 3418S). 30uL of pre-washed Protein G Dynabeads were added to each tube and rotated at 4 °C for 2 hours ...
• KAT6A mutations in Arboleda-Tham syndrome drive epigenetic regulation of posterior HOXC cluster

  Meghna Singh, et al., bioRxiv - Genomics 2023

  Quote: ... The pre-cleared chromatin was then incubated with 5 ul of H3K9ac (Cell signaling #9649), 2 ul of H3K23ac (AbCam #ab177275) ...
• KAT6A mutations drive transcriptional dysregulation of cell cycle and Autism risk genes in an Arboleda-Tham Syndrome cerebral organoid model

  Aileen A. Nava, et al., bioRxiv - Genetics 2023

  Quote: ... Cells were co-stained with a rabbit anti-NANOG (Cell Signaling #4903S) and a mouse anti-OCT3/4 (Santa Cruz #SC5279 ...
• KAT6A mutations drive transcriptional dysregulation of cell cycle and Autism risk genes in an Arboleda-Tham Syndrome cerebral organoid model

  Aileen A. Nava, et al., bioRxiv - Genetics 2023

  Quote: ... and mouse anti-OCT3/4 (Cell Signaling #75463 at 1/1000). The following day ...
• KAT6A mutations drive transcriptional dysregulation of cell cycle and Autism risk genes in an Arboleda-Tham Syndrome cerebral organoid model

  Aileen A. Nava, et al., bioRxiv - Genetics 2023

  Quote: ... rabbit anti-NANOG (Cell Signaling #4903S at 1/1000), and mouse anti-OCT3/4 (Cell Signaling #75463 at 1/1000) ...
• Two independent DNA repair pathways cause mutagenesis in template switching deficient Saccharomyces cerevisiae

  Yangyang Kate Jiang, et al., bioRxiv - Genetics 2023

  Quote: ... used at 1:2,000 dilution) and a monoclonal mouse anti-ubiquitin antibody (Cell Signaling Ubiquitin (P4D1) Mouse mAb ...
• Heat Stress-induced Activation of MAPK Pathway Attenuates Atf1-dependent Epigenetic Inheritance of Heterochromatin in Fission Yeast

  Li Sun, Yamei Wang, Quan-wen Jin, bioRxiv - Genetics 2023

  Quote: ... and rabbit monoclonal phospho-p38 MAPK (Cell Signaling, 4511T) (1:1000) ...
• Novel mouse model of Weaver syndrome displays overgrowth and excess osteogenesis reversible with KDM6A/6B inhibition

  Christine W Gao, et al., bioRxiv - Genetics 2023

  Quote: MEFs were washed twice with cold 1x PBS and lysed using RIPA buffer with a protease/phosphatase inhibitor cocktail (Cell Signaling Technology 5872) to obtain total protein samples ...
• Pharmacologic Targeting of PDIA1 Inhibits NLRP3 Inflammasome Assembly and Activation

  Jessica D. Rosarda, et al., bioRxiv - Cell Biology 2023

  Quote: ... ASC (1:1000, Cell Signaling cat. 13833), IL-1β (1:1000 ...
• Pharmacologic Targeting of PDIA1 Inhibits NLRP3 Inflammasome Assembly and Activation

  Jessica D. Rosarda, et al., bioRxiv - Cell Biology 2023

  Quote: ... CASP1 (1:1000; Cell Signaling cat. 3866), ASC (1:1000 ...
• TANGO-Light - optogenetic control of transcriptional modulators

  Alicja Przybyszewska-Podstawka, et al., bioRxiv - Cell Biology 2023

  Quote: ... Primary anti-FLAG mouse antibody (Cell Signaling Technology, Cat. Nr: #8146), and secondary anti-mouse antibody conjugated with Alexa Fluor 555 dye (Invitrogen Cat ...
• GLUT4 dynamic subcellular localization is controlled by AMP kinase activation as revealed by proximal proteome mapping in human muscle cells

  Anuttoma Ray, et al., bioRxiv - Cell Biology 2023

  Quote: ... phospho-acetyl-CoA carboxylase (Ser79 ACC) (3661, Cell Signaling Technology), phospho T308-AKT (2965S ...
• GLUT4 dynamic subcellular localization is controlled by AMP kinase activation as revealed by proximal proteome mapping in human muscle cells

  Anuttoma Ray, et al., bioRxiv - Cell Biology 2023

  Quote: ... total acetyl-CoA carboxylase (ACC) (3676, Cell Signaling Technology), phospho-acetyl-CoA carboxylase (Ser79 ACC ...
• GLUT4 dynamic subcellular localization is controlled by AMP kinase activation as revealed by proximal proteome mapping in human muscle cells

  Anuttoma Ray, et al., bioRxiv - Cell Biology 2023

  Quote: ... total-AKT (9272S, Cell Signaling Technology), phospho-Thr642 TBC1D4 (8881S ...
• GLUT4 dynamic subcellular localization is controlled by AMP kinase activation as revealed by proximal proteome mapping in human muscle cells

  Anuttoma Ray, et al., bioRxiv - Cell Biology 2023

  Quote: ... phospho T308-AKT (2965S, Cell Signaling Technology), total-AKT (9272S ...
• GLUT4 dynamic subcellular localization is controlled by AMP kinase activation as revealed by proximal proteome mapping in human muscle cells

  Anuttoma Ray, et al., bioRxiv - Cell Biology 2023

  Quote: ... Rab10 (4262S, Cell Signaling Technology), Rab8A (610844 ...
• Phenotyping the virulence of SARS-CoV-2 variants in hamsters by digital pathology and machine learning

  Gavin R Meehan, et al., bioRxiv - Microbiology 2023

  Quote: ... and MX1 (Cell Signaling). As negative control ...
• Cross-family small GTPase ubiquitination by the intracellular pathogen Legionella pneumophila

  Adriana M. Steinbach, et al., bioRxiv - Microbiology 2023

  Quote: ... 10 uL of PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Antibody Bead Conjugate purification (Cell Signaling) slurry was used per 1 mg peptide sample ...
• Nonstructural protein 1 of swine arterivirus PRRSV downregulates promyelocytic leukemia nuclear bodies and promotes viral replication

  Chia-Ming Su, et al., bioRxiv - Microbiology 2023

  Quote: ... Human tumor necrosis factor-α (TNF-α) (8902) was purchased from Cell Signaling (Danvers, MA). DAPI (4′ ...
• CREATION AND CHARACTERIZATION OF A RECOMBINANT MAMMALIAN ORTHOREOVIRUS EXPRESSING HUMAN EPIDERMAL GROWTH FACTOR RECEPTOR 2 PEPTIDES

  Nicole A. Jandick, Cathy L. Miller, bioRxiv - Microbiology 2023

  Quote: ... polyclonal rabbit α-Flag (Cell Signaling DYKDDDK Tag Antibody; #2368), monoclonal mouse α-Flag (Sigma-Aldrich 41106000 M2-Flag) ...
• Mechanistic basis for nigericin-induced NLRP1 inflammasome activation in human epithelial cells

  Pritisha Rozario, et al., bioRxiv - Immunology 2023

  Quote: ... The following antibodies were used in this study: IL1β p17 specific (Cell Signaling Technology, #83186S), pro–IL-1β (R&D Systems ...
• Mechanistic basis for nigericin-induced NLRP1 inflammasome activation in human epithelial cells

  Pritisha Rozario, et al., bioRxiv - Immunology 2023

  Quote: ... Total JNK (Cell Signaling Technology #9252), phospho-JNK (Cell Signaling Technology ...
• Mechanistic basis for nigericin-induced NLRP1 inflammasome activation in human epithelial cells

  Pritisha Rozario, et al., bioRxiv - Immunology 2023

  Quote: ... phospho-p38 (Cell Signaling Technology, #4511), Total JNK (Cell Signaling Technology #9252) ...
• A stress sensor IRE1α is required for bacterial exotoxin-induced inflammasome activation in tissue-resident macrophages

  Izumi Sasaki, et al., bioRxiv - Immunology 2023

  Quote: ... Dishes were stained with anti-IRE1α Ab (3294S, Cell Signaling Technology) or normal rabbit IgG (3900 ...
• A stress sensor IRE1α is required for bacterial exotoxin-induced inflammasome activation in tissue-resident macrophages

  Izumi Sasaki, et al., bioRxiv - Immunology 2023

  Quote: ... or normal rabbit IgG (3900, Cell Signaling Technology) for Isotype control ...
• Host ZCCHC3 blocks HIV-1 infection and production by a dual mechanism

  Binbin Yi, et al., bioRxiv - Microbiology 2023

  Quote: ... HA- and EGFP-tagged proteins were incubated with anti-HA antibody (1: 2,000; 2999, Cell Signaling Technology, Danvers, MA, USA) or anti-EGFP antibody (1 ...
• The pentameric complex is not required for vertical transmission of cytomegalovirus in seronegative pregnant rhesus macaques

  Hsuan-Yuan Wang, et al., bioRxiv - Microbiology 2023

  Quote: ... PE Conjugate (Cell Signaling Technology) to determine the HA tag expression ...
• KSHV vIL-6 Enhances Inflammatory Responses by Epigenetic Reprogramming

  Tomoki Inagaki, et al., bioRxiv - Microbiology 2023

  Quote: ... immunoblotting and flow cytometry: rabbit anti-BRD4 (Cell Signaling, E2A7X), rabbit anti-RNAPII (Millipore ...
• TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells

  D. Wünkhaus, et al., bioRxiv - Cell Biology 2023

  Quote: ... TFEB (Cell Signaling, 4240, 1:100), goat anti-mouse IgG (H+L) ...
• Specific prostaglandins are produced in the migratory cells and the surrounding substrate to promote Drosophila border cell migration

  Samuel Q. Mellentine, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-pMRLC (S19, 1:100; AB_330248; Cell Signaling), mouse anti-Hts (1:50 ...
• The Plasmodium falciparum CCCH zinc finger protein MD3 regulates male gametocytogenesis through its interaction with RNA-binding proteins

  Afia Farrukh, et al., bioRxiv - Microbiology 2023

  Quote: ... Percoll-enriched immature gametocytes were incubated with 0.26 μM TSA (Cell Signaling Technology, Danvers, US) in 0.5% (v/v ...
• Inflammasomes primarily restrict cytosolic Salmonella replication within human macrophages

  Marisa S. Egan, et al., bioRxiv - Microbiology 2023

  Quote: ... and β-actin (4967L; Cell Signaling) and HRP-conjugated secondary antibodies anti-mouse IgG (F00011 ...
• Inflammasomes primarily restrict cytosolic Salmonella replication within human macrophages

  Marisa S. Egan, et al., bioRxiv - Microbiology 2023

  Quote: ... and anti-rabbit IgG (7074S; Cell Signaling) were used ...
• Transgene-Free Ex Utero Derivation of A Human Post-Implantation Embryo Model Solely from Genetically Unmodified Naïve PSCs

  Bernardo Oldak, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Rabbit monoclonal Gata6 (Cell signaling Cat# 5951) 1:200 ...
• The zinc transporter Slc30a1 in macrophages plays a protective role against Salmonella infection

  Pinanong Na-Phatthalung, et al., bioRxiv - Immunology 2023

  Quote: ... or phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (#3033) (all from Cell Signaling Technology). A monoclonal anti-FLAG M2-Peroxidase (HRP ...
• DOT1L interaction partner AF10 controls patterning of H3K79 methylation and RNA polymerase II to maintain cell identity

  Coral K. Wille, et al., bioRxiv - Genetics 2023

  Quote: ... H3K27me3 (1:1000; Cell Signaling, 9733S), total Histone H3 (1:3000 ...