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• Prediction of Resistant Mutations against Upcoming ALK-TKIs, Repotrectinib (TPX-0005) and Ensartinib (X-396)

  Yuta Doi, et al., bioRxiv - Cancer Biology 2022

  Quote: ... HRP-linked antibody (Cell Signaling Technologies, #7074, 1:2000) and anti-mouse IgG ...
• Prediction of Resistant Mutations against Upcoming ALK-TKIs, Repotrectinib (TPX-0005) and Ensartinib (X-396)

  Yuta Doi, et al., bioRxiv - Cancer Biology 2022

  Quote: ... HRP-linked antibody (Cell Signaling Technologies, #7076, 1:2000). Then the membranes were washed with TBST and incubated with Immobilon® Western Chemiluminescent HRP Substrate (EMD Millipore Corporation ...
• Focal adhesion-based cell migration is differentially regulated in vivo versus in vitro by Paxillin phosphorylation

  Qian Xue, et al., bioRxiv - Cell Biology 2022

  Quote: ... Rabbit anti-pFAK397 (Cell Signaling Technology, 3283, 1:1000), Chicken anti-GFP (Abcam ...
• Focal adhesion-based cell migration is differentially regulated in vivo versus in vitro by Paxillin phosphorylation

  Qian Xue, et al., bioRxiv - Cell Biology 2022

  Quote: ... Rabbit anti-FAK (Cell Signaling Technology, 3285, 1:1000), Rabbit anti-pFAK397 (Cell Signaling Technology ...
• On optimal temozolomide scheduling for slowly growing gliomas

  Berta Segura-Collar, et al., bioRxiv - Cancer Biology 2022

  Quote: ... rabbit anti-pTyr1068-EGFR (1:1.000, Cell Signaling #3777) and rabbit anti-phospho-NF-kB p65 (Ser536 ...
• Profiling DNA break sites and transcriptional changes in response to contextual fear learning

  Ryan T. Stott, Oleg Kritsky, Li-Huei Tsai, bioRxiv - Neuroscience 2021

  Quote: ... GFAP AF647 (1:200; clone GA5; Cell Signaling Technology), and PU.1 PE (1:200 ...
• GDAP1 loss of function inhibits the mitochondrial pyruvate dehydrogenase complex by altering the actin cytoskeleton

  Christina Wolf, et al., bioRxiv - Neuroscience 2021

  Quote: ... anti-p-PDH (Ser293, 1:1000, Cell Signaling 31866), anti-PKM1/2 mAb (clone C103A3 ...
• Oligodendrocyte progenitor proliferation is disinhibited following traumatic brain injury in LIF heterozygous mice

  Michelle J. Frondelli, et al., bioRxiv - Neuroscience 2020

  Quote: ... & total S6RP (1:1000, Cell Signaling Technology, 2217, RRID:AB_331355) overnight at 4°C ...
• Interferon signaling suppresses the unfolded protein response and induces cell death in hepatocytes accumulating hepatitis B surface antigen

  Ian Baudi, et al., bioRxiv - Microbiology 2020

  Quote: ... phospho-Stat-1 (#9167) (all from Cell Signaling Technologies), GAPDH (abcam#8245) ...
• FAM72A antagonizes UNG2 to promote mutagenic uracil repair during antibody maturation

  Yuqing Feng, et al., bioRxiv - Immunology 2020

  Quote: ... rabbit anti-His tag antibody (Cell signaling; 1:2000), and anti-UDG antibody (Santa Cruz ...
• Metalloproteinase-dependent and TMPRSS2-independnt cell surface entry pathway of SARS-CoV-2 requires the furin-cleavage site and the S2 domain of spike protein

  Mizuki Yamamoto, et al., bioRxiv - Microbiology 2021

  Quote: ... rabbit anti-ADAM10 (1:1000, 14194S, Cell Signaling Technology), rabbit anti-Flag-tag (1:1000 ...
• Bab2 activates JNK signaling to reprogram Drosophila wing disc development

  Yunpo Zhao, et al., bioRxiv - Developmental Biology 2020

  Quote: ... rabbit anti-Cleaved Caspase 3 1:300 (Cell Signaling), rat anti-Bab2 1:10000 (Jean-Louis Couderc) ...
• RAB6 GTPase is a crucial regulator of the mammary secretory function controlling STAT5 activation

  Surya Cayre, et al., bioRxiv - Developmental Biology 2020

  Quote: ... anti-RAB5 (Cell Signaling Technology, CST; #3547S; 1/1000), anti-RAB8 (BD Biosciences ...
• Sperm-specific COX6B2 enhances oxidative phosphorylation, proliferation, and survival in lung adenocarcinoma

  Chun-Chun Cheng, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Cleaved Caspase-3 (1:500, 9661, Cell Signaling Technology), PARP (1:1000 ...
• A ubiquitin-based mechanism for the oligogenic inheritance of heterotaxy and heart defects

  Jennifer H. Kong, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-GLI1 (clone L42B10, Cell Signaling, 1:1000); mouse anti-HA.11 (clone 16B12 ...
• Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD

  Jaspreet K. Osan, et al., bioRxiv - Microbiology 2020

  Quote: ... anti-MUC5AC rabbit monoclonal (1:200) (Cell Signaling Technology), anti-acetyl-alpha-tubulin rabbit monoclonal (1:500 ...
• Partial Inhibition of Mitochondrial Complex I Reduces Tau Pathology and Improves Energy Homeostasis and Synaptic Function in 3xTg-AD Male and Female Mice

  Andrea Stojakovic, et al., bioRxiv - Neuroscience 2020

  Quote: ... p35/p25 (1:1000, Cell Signaling Technology, cat. # 2680), GSK3β (1:1000 ...
• Reduction of Glut1 in retinal neurons but not the RPE alleviates polyol accumulation and normalizes early characteristics of diabetic retinopathy

  Nicholas C. Holoman, et al., bioRxiv - Neuroscience 2020

  Quote: ... and Mo anti-actin (Cell signaling #3700S, 1:1000). PVDF membranes were scanned with an Odyssey infrared scanner and densitometry was performed using LiCor Image Studio Software.
• SerpinB3 drives cancer stem cell survival in glioblastoma

  Adam Lauko, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and c-Myc (Cell Signaling Technology 5605, 1:5000). β-Actin (Bio-Rad 12004163 ...
• A chemical screen identifies a link between lipid metabolism and mRNA translation

  Alba Corman, et al., bioRxiv - Molecular Biology 2020

  Quote: ... 4E-BP1 (1:1000, CST Cell Signaling Tech, 9452S), p-eIF2a (phospho S51 ...
• Dynamin-related Irgm proteins modulate LPS-induced caspase-4 activation and septic shock

  Ryan Finethy, et al., bioRxiv - Immunology 2020

  Quote: ... rabbit anti-LC3 (2775; Cell Signaling Technology; 1:500), goat anti-CD14 (AF982 ...
• TGF-β-driven downregulation of the Wnt/β-Catenin transcription factor TCF7L2/TCF4 in PDGFRα⁺ fibroblasts

  Osvaldo Contreras, et al., bioRxiv - Cell Biology 2020

  Quote: ... rabbit anti-β-Catenin (1:2000; 9562, Cell Signaling), rabbit anti-histone 3 H3 (1:1000 ...
• Anti-inflammatory role of GM1 and modulatory effects of gangliosides on microglia functions

  Danny Galleguillos, et al., bioRxiv - Neuroscience 2020

  Quote: ... rabbit anti-p38 MAPK (Cell Signaling 9212; 1:1,000), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182 ...
• Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging

  Stephanie K. Bonney, et al., bioRxiv - Neuroscience 2021

  Quote: ... or rabbit anti-Pdgfrα (1:100; Cell signaling; D1E1E) overnight at 4°C ...
• A novel cholinergic neural pathway and its role in the drug relapse

  Teng He, et al., bioRxiv - Neuroscience 2021

  Quote: ... rabbit anti-NeuN (1:200, Cell Signaling Technology, USA) at 4 °C for 24 h ...
• Dysregulation of NRSF/REST via EHMT1 is associated with psychiatric disorders

  Mouhamed Alsaqati, et al., bioRxiv - Neuroscience 2021

  Quote: ... Oct-4 (1:200, 2750, Cell Signaling Technology, USA), Sox2 (1:200 ...
• Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, while the Alzheimer’s R47H mutant does the opposite

  Anna Vilalta, et al., bioRxiv - Biochemistry 2020

  Quote: ... GAPDH (#2118, 1:3000 for western blot; Cell Signaling); Anti-His (C-term)-HRP (#46-0707 ...
• Identification and Characterization of a B-Raf Kinase Alpha Helix Critical for the Activity of MEK Kinase in MAPK Signaling

  Diep Nguyen, et al., bioRxiv - Biochemistry 2020

  Quote: ... or anti-GST antibody (1:1000) (Cell Signaling, #2625). The next day ...
• Angiocrine IGFBP3 Spatially Coordinates IGF Signaling During Neonatal Cardiac Regeneration

  Shah R. Ali, et al., bioRxiv - Cell Biology 2021

  Quote: ... anti-phospho Akt (S473) (Cell Signaling, 4060S, 1:1000), anti-Myosin Light Chain 2/MLC-2V (Proteintech ...
• S-nitrosylated and non-nitrosylated COX2 has differential expression and distinct subcellular localization in normal and breast cancer tissue

  Sonali Jindal, et al., bioRxiv - Cancer Biology 2020

  Quote: ... COX-1 (Cell Signaling Technology # 4841, at 25ng/uL) and GAPDH (14C10 clone ...
• Epithelial-mesenchymal plasticity induced by discontinuous exposure to TGFβ1 promotes tumour growth

  Mafalda Santos, et al., bioRxiv - Cell Biology 2021

  Quote: ... incubated with anti-Snail (1:50,Cell Signaling,USA), anti-MMP2 (1:50,Calbiochem,USA ...
• Inhibiting proBDNF to mature BDNF conversion leads to autism-like phenotypes in vivo

  He You, et al., bioRxiv - Neuroscience 2020

  Quote: ... anti-SynGAP (1:1000; clone D88G1; Cell Signaling Technology), anti-GluN2B (1:1000 ...
• Global kinome silencing combined with 3D invasion screening of the tumor microenvironment identifies fibroblast-expressed PIK3Cδ involvement in triple-negative breast cancer progression

  Teresa Gagliano, et al., bioRxiv - Cancer Biology 2019

  Quote: ... pAkt-S473 (Cell Signaling Technology, CST, 4060, 1:100), pAkt-Thr308 (Millipore ...
• Both DNA binding domains of p53 are required for its ultra-rapid recruitment to sites of UV damage

  YH. Wang, et al., bioRxiv - Cell Biology 2020

  Quote: ... and p53 [clone DO-1] (Cell Signaling Technology #18032); followed by co-staining of secondary antibodies including AlexaFluor488-Goat anti-mouse IgG (A11001 ...
• The hallucinogenic serotonin_2A receptor agonist, DOI, promotes CREB-dependent gene expression of specific plasticity-associated genes in the rodent neocortex

  Lynette A. Desouza, et al., bioRxiv - Neuroscience 2021

  Quote: ... and rabbit anti-CREB (1:1000, Cell Signaling Technology). Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare ...
• “A feed-forward Ca²⁺-dependent mechanism boosting glycolysis and OXPHOS by activating Aralar-malate-aspartate shuttle, upon neuronal stimulation”

  Irene Pérez-Liébana, et al., bioRxiv - Neuroscience 2021

  Quote: ... anti-p-AMPK T172 (1:1,000, Cell Signaling Technology), anti-β-F1ATPase (1:5,000 ...
• Comparing synaptic proteomes across seven mouse models for autism reveals molecular subtypes and deficits in Rho GTPase signaling

  Abigail U. Carbonell, et al., bioRxiv - Neuroscience 2021

  Quote: ... and rat anti-tubulin 1:1000 (Cell Signaling Tech). Statistical analysis for Western blot was performed in JMP 14 (SAS ...
• A Complex Containing HNRNPA2B1 and N⁶-methyladenosine Modified Transcripts Mediates Actions of Toxic Tau Oligomers

  Lulu Jiang, et al., bioRxiv - Neuroscience 2020

  Quote: ... 1:500 (Cell Signaling Technology, Cat# 3597, RRID: AB_390740); Rabbit Polyclonal anti-eIF3l ...
• JAG1 intracellular domain acts as a transcriptional cofactor that forms an oncogenic transcriptional complex with DDX17/SMAD3/TGIF2

  Eun-Jung Kim, et al., bioRxiv - Molecular Biology 2021

  Quote: ... anti-HA (1:1000, Cat. #3724s; Cell Signaling Technology), anti-LAMIN B2 (1:500 ...
• α-Synuclein facilitates endocytosis by elevating the steady-state levels of phosphatidylinositol 4,5-bisphosphate

  Schechter Meir, et al., bioRxiv - Neuroscience 2020

  Quote: ... P-AP2M1-T156 (D4F3; Cell Signaling Technology; 1:300). The following antibodies were from Echelon Biosciences (Salt Lake City ...
• Homeostatic Plasticity Requires Remodeling of the Homer-Shank Interactome

  Whitney E. Heavner, et al., bioRxiv - Neuroscience 2020

  Quote: ... and total ERK (9102, Cell Signaling Technologies, 1:1000). Primary antibodies were detected using species-specific HRP-conjugated secondary antibodies ...
• The m⁶A reader YTHDF2 is a negative regulator for dendrite development and maintenance of retinal ganglion cells

  Fugui Niu, et al., bioRxiv - Neuroscience 2021

  Quote: ... rabbit anti-PKCα (1:1000, Cell Signaling CST-2056), rabbit anti-PSD95 (1:1000 ...
• A Locus Coeruleus- dorsal CA1 dopaminergic circuit modulates memory linking

  Ananya Chowdhury, et al., bioRxiv - Neuroscience 2021

  Quote: ... rabbit anti-cFos (Cell Signaling, 9F6, #2250, 1:500), chicken anti-TH (Abcam AB76442) ...
• Dose-dependent phorbol 12-myristate-13-acetate-mediated monocyte-to-macrophage differentiation induces unique proteomic signatures in THP-1 cells

  Sneha M. Pinto, et al., bioRxiv - Immunology 2020

  Quote: ... anti- IL1B (1:1000; cat#12242; Cell Signaling Technology) and SQSTM1 (1:1000 ...
• Dose-dependent phorbol 12-myristate-13-acetate-mediated monocyte-to-macrophage differentiation induces unique proteomic signatures in THP-1 cells

  Sneha M. Pinto, et al., bioRxiv - Immunology 2020

  Quote: ... anti-TBK1 (1:1000; cat#3504; Cell Signaling Technology), anti- IL1B (1:1000 ...
• A Focused Small-Molecule Screen Identifies PHA-680626 as an Amphosteric Inhibitor Disrupting the Interaction between Aurora-A and N-Myc

  Fani Souvalidou, et al., bioRxiv - Cancer Biology 2021

  Quote: ... rabbit anti-PARP (9542, Cell Signaling, 1:1000 o.n.). HRP-conjugated secondary antibodies (BioRad ...
• Sortilin exhibits tumor suppressor-like activity by limiting EGFR transducing function

  E. Lapeyronnie, et al., bioRxiv - Cancer Biology 2021

  Quote: ... pAKT (Ser 473, #4060, 1:1000 dilution, Cell Signaling), AKT (#4691 ...
• Automated compound testing in zebrafish xenografts identifies combined MCL-1 and BCL-X_L inhibition to be effective against Ewing sarcoma

  Sarah Grissenberger, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and GAPDH (1:5000, rabbit, 5174S, Cell Signaling, UK) and secondary antibody α-rabbit (1:5000 ...
• The long non-coding RNA SAMMSON is essential for uveal melanoma cell survival

  Shanna Dewaele, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and anti-mouse (7076P2, Cell Signaling, 1:10000 dilution) antibodies were used as secondary antibodies ...
• The DNA methyltransferase 1 (DNMT1) acts on neurodegeneration by modulating proteostasis-relevant intracellular processes

  Cathrin Bayer, et al., bioRxiv - Neuroscience 2020

  Quote: ... rabbit-anti-LC3B (#2775S, Cell Signaling Technology, 1:1000). The following secondary antibodies coupled to horseradish peroxidase (HRP ...