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• Cystin is required for maintaining fibrocystin (FPC) levels and safeguarding proteome integrity in mouse renal epithelial cells A mechanistic connection between the kidney defects in cpk mice and human ARPKD

  Yiming Zhang, et al., bioRxiv - Cell Biology 2023

  Quote: ... polyubiquitin K48 (rabbit pAb, Cell Signaling; 4289; 1:1000), ENaCα (rabbit pAb ...
• Rational development of a small-molecule activator of CK1γ2 that decreases C99 and beta-amyloid levels

  Victor Hugo Bustos, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... anti-p62 (rabbit polyclonal, 1:500; Cell Signaling Technology), anti-CK1γ2 (rabbit polyclonal ...
• Repair oligodendrocytes demyelinating and disintegrating damaged axons after injury

  Gianluigi Nocera, et al., bioRxiv - Neuroscience 2023

  Quote: ... c-Jun (Rabbit, 1:200, Cell signaling, 60A8, #9165), c-Jun (mouse ...
• Repair oligodendrocytes demyelinating and disintegrating damaged axons after injury

  Gianluigi Nocera, et al., bioRxiv - Neuroscience 2023

  Quote: ... c-Jun (Rabbit, 1:500, Cell signaling, 60A8, #9165), Phospho-c-Jun (Rabbit ...
• The hepatokine FGL1 regulates hepcidin and iron metabolism during the recovery from hemorrhage-induced anemia in mice

  Ugo Sardo, et al., bioRxiv - Physiology 2023

  Quote: ... goat anti-rabbit IgG (Cell signaling, 7074, 1/10000) or horse anti-mouse IgG (Cell signaling ...
• Predicting gene level sensitivity to JAK-STAT signaling perturbation using a mechanistic-to-machine learning framework

  Neha Cheemalavagu, et al., bioRxiv - Systems Biology 2023

  Quote: ... anti-pSTAT3 Y705 (Cell Signaling Technology 4113, 1:200), and anti-pSTAT1 Y701 (Cell Signaling Technology 9167 ...
• Mechanistic characterization of a Drosophila model of paraneoplastic nephrotic syndrome

  Jun Xu, et al., bioRxiv - Zoology 2023

  Quote: ... rabbit anti-ERK Antibody (Cell Signaling, 4695, 1:1000), rabbit phospho-ERK (Cell Signaling ...
• Ca²⁺/Calmodulin Dependent Protein Kinase Kinase-2 (CaMKK2) promotes Protein Kinase G (PKG)-dependent actin cytoskeletal assembly to increase tumor metastasis

  Debarati Mukherjee, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-Actin (1:10000, 8H10D10, Catalog # 3700, Cell Signaling). Secondary antibodies used were HRP-conjugated anti-mouse IgG (1:5000 ...
• Pan-cancer analysis of the ion permeome reveals functional regulators of glioblastoma aggression

  Alexander T. Bahcheli, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and rabbit anti-GAPDH (1:3000, Cell Signaling #2118S). Experiments were performed in three biological replicates ...
• SLC15A4 controls endolysosomal TLR7-9 responses by recruiting the innate immune adaptor TASL

  Haobo Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... rabbit anti-HA (no. 3724, Cell Signaling, 1:1,000) and mouse anti-LAMP2 (sc-18822 ...
• Fibronectin, DHPS and SLC3A2 Signaling Cooperate to Control Tumor Spheroid Growth, Subcellular eIF5A1/2 Distribution and CDK4/6 Inhibitor Resistance

  Cameron Geller, et al., bioRxiv - Cancer Biology 2023

  Quote: ... or SMAD3 (Cell Signaling Technology catalog #9523S, 1:100) in 2% BSA/PBS solution and the appropriate fluorescently-conjugated secondary antibody (R&D Systems catalog #NL006 ...
• Deletion of smooth muscle O-GlcNAc transferase prevents development of atherosclerosis in western diet-fed hyperglycemic ApoE^-/- mice in vivo

  Saugat Khanal, et al., bioRxiv - Pathology 2023

  Quote: ... pERK and tERK (1:1000, Cell Signaling, Danvers, MA); O-GlcNAc (RL2 ...
• Primary cilia promote the differentiation of human neurons through the WNT signaling pathway

  Andrea Coschiera, et al., bioRxiv - Neuroscience 2023

  Quote: ... and rabbit anti-PSD95 (Cell Signaling 2507; 1:100). Centrosome (centriole ...
• Astrocytic LRRK2 Controls Synaptic Connectivity through ERM Phosphorylation

  Shiyi Wang, et al., bioRxiv - Neuroscience 2023

  Quote: ... and phospho-ERM (Rabbit, 1:250; #3726, Cell Signaling). Following primary incubation ...
• Loss of presenilin-1 in smooth muscle cells ameliorates elastin aortopathy

  Junichi Saito, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-HES1 (Cell Signaling Technology, 11988; 1:1000), rabbit anti-Ki67 (Invitrogen ...
• mRNA Location and Translation Rate Determine Protein Targeting to Dual Destinations

  Alexander N. Gasparski, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-GAPDH (1:2000; Cell Signaling cat# 2118L). Anti-rabbit and anti-mouse secondary antibodies from Li-Cor were used at 1:10,000 ...
• Stem cells tightly regulate dead cell clearance to maintain tissue fitness

  Katherine S Stewart, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-RARγ (D3A4) (Cell Signaling, 8965, 1:250), rabbit anti-MFGE8 (Invitrogen ...
• p90RSK2, a new MLCK, rescues contractility in myosin light chain kinase null smooth muscle

  Jaspreet Kalra, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-Pyk2 phospho-Tyr402 (1:500) from Cell Signaling. For triple westerns proteins were separated on 1.5 mm thick ...
• The effect of estradiol during the early stages of osteoclast differentiation is associated with the accumulation of phosphorylated p53 in mitochondria and the inhibition of mitochondrial metabolism

  Adriana Marques-Carvalho, et al., bioRxiv - Cell Biology 2023

  Quote: ... Bax (1:1000, 2772, Cell Signaling, Danvers, MA, USA), denaturated form of OXPHOS complexes cocktail (1:1000 ...
• The mitotic surveillance pathway requires PLK1-dependent 53BP1 displacement from kinetochores

  Matteo Burigotto, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-53BP1 (Cell Signaling Technology, 4937, 1:500), mouse anti-cyclin A2 (Abcam ...
• Host genetic background regulates the capacity for anti-tumor antibody-dependent phagocytosis

  James E. Glassbrook, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and CD8α (1:400, tris, Cell Signaling Technology, 98941). Tumors were harvested ...
• NF-κB c-REL-OTUD4 axis regulates B-cell receptor in B-cell lymphoma

  Eslam Katab, et al., bioRxiv - Cancer Biology 2023

  Quote: ... c-REL (1:1,000, rabbit, Cell Signaling Technology #4727), CUL1 (1:500 ...
• Senescent cells and macrophages cooperate through a multi-kinase signaling network to promote intestinal transformation in Drosophila

  Ishwaree Datta, Erdem Bangi, bioRxiv - Cancer Biology 2023

  Quote: ... rabbit anti-Dcp1(1:1000, Cell Signaling Technology #9578), mouse anti-β-gal (1:100 ...
• Bcl-xL is translocated to the nucleus via CtBP2 to epigenetically promote metastasis

  Tiantian Zhang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Myc tag (1:1,000, Cell Signaling Technology, Cat# 2276), V5 (1:1,000 ...
• Human USP18 protects diverse cancer lineages from Type I Interferon independently of its canonical catalytic function

  Veronica Jové, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rabbit anti-USP18 (D4E7) 1:25 (Cell Signaling, 4813S), and rabbit anti-Vinculin 1:300 (Abcam ...
• Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastoma

  Lise Finotto, et al., bioRxiv - Cancer Biology 2023

  Quote: ... with anti-GAL1 (Cell Signaling Technology, 13888, 1:375) and anti-SOX2 (ThermoFisher Scientific ...
• Bcl-xL is translocated to the nucleus via CtBP2 to epigenetically promote metastasis

  Tiantian Zhang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Mek1/2 (1:1,000, Cell Signaling Technology, Cat# 9122), alpha-tubulin (Sigma ...
• Lack of mTORC2 signaling in CD11c⁺ myeloid cells inhibits their migration and ameliorates experimental colitis

  Aline Ignacio, et al., bioRxiv - Immunology 2023

  Quote: ... -pS6 (T389) (1:1.000, Cell Signaling Technology, MA, USA), -Claudin 2 (1:500) ...
• RSV infection does not induce EMT

  Sattya N. Talukdar, et al., bioRxiv - Microbiology 2023

  Quote: ... rabbit monoclonal (1:1000) E-cadherin (Cell Signaling Technologies), and rabbit monoclonal (1:1000 ...
• DISTINCT ROLES OF VIRAL US3 AND UL13 PROTEIN KINASES IN HERPES VIRUS SIMPLEX TYPE 1 (HSV-1) NUCLEAR EGRESS

  Masoudeh Masoud Bahnamiri, Richard J. Roller, bioRxiv - Microbiology 2023

  Quote: ... rabbit anti-phospho-Akt substrates (1:500) (Cell Signaling), mouse monoclonal anti-actin (Sigma-Aldrich) ...
• PARP inhibition and pharmacological ascorbate demonstrate synergy in castration-resistant prostate cancer

  Nicolas Gordon, et al., bioRxiv - Cancer Biology 2023

  Quote: ... phospho-CDC2 (Tyr15) (1:1500, Cell Signaling Technology 9111S), CDC2 p34 (1:200 ...
• Wnt5a-Ror2 signaling activates p62-Nrf2 axis in reactive astrocytes after brain injury

  Mitsuharu Endo, et al., bioRxiv - Molecular Biology 2023

  Quote: ... rabbit anti-Wnt5a (Cell Signaling Technology, 2530, 1:1000), mouse anti-α-Tubulin (Sigma-Aldrich ...
• Wnt5a-Ror2 signaling activates p62-Nrf2 axis in reactive astrocytes after brain injury

  Mitsuharu Endo, et al., bioRxiv - Molecular Biology 2023

  Quote: ... rabbit anti-Nrf2 (Cell Signaling Technology, 12721, 1:500), and rabbit anti-Pgd (Proteintech ...
• Wnt5a-Ror2 signaling activates p62-Nrf2 axis in reactive astrocytes after brain injury

  Mitsuharu Endo, et al., bioRxiv - Molecular Biology 2023

  Quote: ... rabbit anti-NBR1 (Cell Signaling Technology, 20145, 1:1000), mouse anti-Pgd (Santa Cruz Biotechnology ...
• A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB

  Rachel E. Cherney, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Antibodies used were: EZH2 (Cell Signaling #5246, 1:1000), EZH2 (Cell Signaling #3147 ...
• The TRIP6/LATS1 complex constitutes the tension sensor of α-catenin/vinculin at both bicellular and tricellular junctions

  Lin Xie, et al., bioRxiv - Cell Biology 2023

  Quote: ... HRP-linked Antibody (Cell Signaling technology, 7074S, 1:4000), Anti-mouse IgG ...
• Phencyclidine disrupts neural coordination and cognitive control by dysregulating translation

  Eun Hye Park, et al., bioRxiv - Neuroscience 2023

  Quote: ... phospho-AKT (Cell Signaling Technology, Cat.No.9271S, 1:1000), AKT (Cell Signaling Technology ...
• Phencyclidine disrupts neural coordination and cognitive control by dysregulating translation

  Eun Hye Park, et al., bioRxiv - Neuroscience 2023

  Quote: ... phospho-4EBP1 (Cell Signaling Technology, Cat.No.9459S, 1:1000), 4EBP1 (Cell Signaling Technology ...
• EZH2 deletion does not impact acinar cell regeneration but restricts progression to pancreatic cancer in mice

  Emilie Jaune-Pons, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rabbit α-SMA (Cell signaling technology, #19245, 1:200), rabbit Vimentin (Cell signaling technology ...
• EZH2 deletion does not impact acinar cell regeneration but restricts progression to pancreatic cancer in mice

  Emilie Jaune-Pons, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rabbit Total ERK (Cell signaling technology, #9102, 1:1000). After washing ...
• The response of Dual-leucine zipper kinase (DLK) to nocodazole: evidence for a homeostatic cytoskeletal repair mechanism

  Laura DeVault, et al., bioRxiv - Neuroscience 2023

  Quote: ... 1:1000 rabbit anti-cJun (Cell Signaling Technologies 9165P), 1:1000 rabbit anti-HSP90 (Cell Signaling Technologies 4877S) ...
• The response of Dual-leucine zipper kinase (DLK) to nocodazole: evidence for a homeostatic cytoskeletal repair mechanism

  Laura DeVault, et al., bioRxiv - Neuroscience 2023

  Quote: ... 1:1000 rabbit anti-HSP90 (Cell Signaling Technologies 4877S). Goat Anti-Rabbit IgG (Jackson ImmunoResearch Laboratories 111–035-045 ...
• Death Induced by Survival gene Elimination (DISE) is correlated with neurotoxicity in Alzheimer’s disease and aging

  Bidur Paudel, et al., bioRxiv - Neuroscience 2023

  Quote: ... Drosha (D28B1) rabbit mAb (Cell Signaling #3364, 1:1000), Dicer (D38E7 ...
• APC/C prevents non-canonical order of cyclin/CDK activity to maintain CDK4/6 inhibitor-induced arrest

  Brandon L Mouery, et al., bioRxiv - Cell Biology 2023

  Quote: ... p27 (1:1000; rabbit; 3686; Cell Signaling Technology; BSA), Cyclin B1 (1:1000 ...
• APC/C prevents non-canonical order of cyclin/CDK activity to maintain CDK4/6 inhibitor-induced arrest

  Brandon L Mouery, et al., bioRxiv - Cell Biology 2023

  Quote: ... E2F1 (1:1000; rabbit; 3742; Cell Signaling Technology; BSA), E2F2 (1:1000 ...
• c-Src induced vascular malformations require localised matrix degradation at focal adhesions

  Patricia Essebier, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-paxillin (Cell Signaling, 2542, WB 1:1000), rabbit anti- phospho-FAK (Tyr576 ...
• Obesogenic diet in mice leads to inflammation and oxidative stress in the mother in association with sex-specific changes in fetal development, inflammatory markers and placental transcriptome

  Alejandro A. Candia, et al., bioRxiv - Physiology 2023

  Quote: ... 3-nitrotyrosine (3-NT, #9691 Cell signaling, 1:10000), 4-hydroxynonenal (4HNE ...
• CCR2-targeting pepducins reduce T cell-nociceptor interaction driving bone cancer pain

  Élora Midavaine, et al., bioRxiv - Neuroscience 2023

  Quote: ... and mouse anti-pJNK (1:100, Cell Signaling #9251), chicken anti-CCR2 (1:200 ...
• Tenofovir Activation is Diminished in the Brain and Liver of Creatine Kinase Brain-Type Knockout Mice

  Colten D. Eberhard, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... and 1:1,000 anti-GAPDH (#5174, Cell Signaling Technologies) in 5% bovine serum albumin in TBST at 4°C overnight ...
• Oligodendrocyte maturation alters the cell death mechanisms that cause demyelination

  Timothy W. Chapman, et al., bioRxiv - Neuroscience 2023

  Quote: ... Rabbit anti-gammaH2AX (Cell Signaling, 1:500, Cat#9718). All staining was done using 0.3% TritonX100 in PBS with 1% Bovine Serum Albumin ...