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Citations for Cell Signaling Technology :
6451 - 6500 of 10000+ citations for Dl Serine 1 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were subsequently blocked with 5% milk or 5% bovine serum albumin for 1 h at room temperature and probed separately with specific primary antibodies to AKT1 (1:1,000 dilution, 75692, Cell Signaling Technology, Danvers, MA), pan-AKT (1:1,000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were washed and then blocked using 1% Goat serum in TBS for one hour at room temperature before adding primary antibodies to detect Cd45 (Cell Signaling, 1:500) and Cd11b (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-otoferlin (Developmental Studies Hybridoma Bank, HCS-1) diluted at 1:100 and rabbit anti-cleaved caspase-3 (Cell Signaling Technology, 9661) diluted at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... or 5% non-fat dry milk in TBST for 1 h then incubated overnight at 4°C in blocking buffer containing antibodies against TMEM106B (1:500, Cell Signaling Technology 93334), TDP-43 (1:1,500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% non-fat dry milk in TBST for 1 h and then incubated overnight at 4°C in blocking buffer containing the antibody against TMEM106B (1:500, Cell Signaling Technology 93334). The secondary antibody ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... the sections were subjected to antibody immunofluorescent staining (pS6 Cell Signaling 2215, 1:1000; GFAP Cell Signaling #3670, 1:500; NeuN Cell Signaling D4G40 ...
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bioRxiv - Immunology 2022Quote: ... Intracellular phospho-stainings were performed 1 h at room temperature in the dark with antibodies against phospho-STAT5 (Tyr694; D47E7 XP Rabbit mAb #4322; Cell Signaling, 1:150), phospho-S6 ribosomal protein (Ser235/236 ...
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bioRxiv - Genomics 2023Quote: ... Western blotting was performed according to standard procedures using the following antibodies and dilutions: TP53 (DO-1, 1:500, Cell Signaling #18032, mouse), CDKN1A (F-5 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then blocked with immunofluorescence (IF) block buffer (3% BSA in PBS) for 1 hour and incubated with primary antibodies: rabbit anti-γH2A.x (Cell Signaling 9718, 1:2000), rabbit anti-RPA32 (GeneTex GTX113004 1:2000) ...
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bioRxiv - Cell Biology 2023Quote: ... 2μg/mL, Ab189034, Waltham, MA 02453, USA), Klf4 (1 μg/mL, CST4038T) and β-actin (1 μg/mL, 4967S) from Cell Signaling Technology, Danvers ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were blocked with 3% BSA in PBS for 1 hour and cells were stained with VEGFR2 (Cell Signaling; C#2479; 1:200) and EEA1 (R&D Systems ...
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Specific patterns of neural activity in the hippocampus after massed or distributed spatial trainingbioRxiv - Neuroscience 2023Quote: ... After 1 h of incubation in PBST containing 1% BSA and 1% NGS sections were incubated overnight in anti-phospho-c-Fos rabbit monoclonal antibody (5348S; Cell signaling Technology, USA) diluted 1:8000 in PBST– BSA–NGS ...
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bioRxiv - Bioengineering 2023Quote: ... Staining was performed by incubating the cells overnight at 4°C with primary antibodies diluted in 1% BSA targeting α-smooth muscle actin (1:200; 48938S, Cell Signaling Technology), gamma actin (1:500 ...
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bioRxiv - Genomics 2023Quote: ... Cells were proceeded for IF as described above using anti-CD56 mouse antibodies (1:10, Coulter #PN4235479-G) and anti-TCF7L2 rabbit antibodies (1:100, Cell Signaling Technology #C48H11), revealed by Cy3-coupled anti-mouse IgGs and Cy5-coupled anti-rabbit IgGs.
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Neuroscience 2024Quote: ... and 2 h later they were labeled with mouse anti-Iba1 (1:500; Fujifilm Wako Chemicals, VA, USA) or rabbit anti-p75NTR (1:750; Cell signaling, MA, USA) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were rat anti-ATF4 (1:200, (Hunt et al., 2019)) and rabbit anti-P-eIF2α (1:500, anti-Phospho-eIF2α [Ser51], Cell Signaling Technology 9721). Secondary antibodies were goat anti-rabbit Alexa Fluor 546 (Invitrogen A11035 ...
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bioRxiv - Bioengineering 2023Quote: ... secondary antibodies were incubated for 1 hour at room temperature (Secondary antibodies: Anti-Rabbit HRP-conjugated Cell Signaling Technology (CST-#7074S 1:5000); Anti-Mouse HRP-conjugated (R&D ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 hour and then incubated with a 1:250 dilution of monoclonal rabbit F4/80 or 1:150 of rabbit Ly-6G (Cell Signaling, Danvers, MA) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti phospho-myosin light chain 2 Thr18/Ser19 (pMLC, 1:50 for IF, 1:20 for IG) was from Cell Signaling Technologies (#3674). Chicken anti-map2 (against residues 2-314 of human map2 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were incubated for 1 h at room temperature with appropriate secondary antibodies: goat anti-rabbit (1:2000, Cell Signaling Technology, Cat. 7074), goat anti-rat (1:10000 ...
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bioRxiv - Physiology 2024Quote: ... and /or anti-TOM20 antibody (diluted 1:1000, SantaCruz; Cat#sc177615) or/and anti-DRP1 antibody (diluted 1:50 Cell signaling; Cat#8570). After several washes with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The blots were probed with the following primary antibodies and 0.2% Tween20 with overnight incubation at 4°C: anti-p-eIF2α (Abcam; ab32157; 1:1000)(Cell Signaling; 3398s; 1:1000), anti-total-eIF2α (Cell Signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% BSA in TBS for 1 hr and then incubated overnight at 4° C with anti-pSMAD1/5/8 (1:1000, Cell Signaling Technology, 13820), anti-SMAD1 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% Triton X-100 in PBS) for 1 hour at room temperature and with primary antibodies anti-Calbindin (Cell Signaling, 2173, 1:100), anti-KDEL (Novus Biologicals ...
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bioRxiv - Microbiology 2020Quote: ... for evaluation of histopathologic changes by H&E staining or for immunohistochemical detection of reovirus antigen (1:45,000 dilution) or the cleaved (active) form of caspase-3 (Cell Signaling, 9664; 1:300 dilution). Images were captured at a magnification of ×20 and a resolution of 0.5 μm/pixel using a high-throughput Leica SCN400 slide scanner automated digital imaging system.
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bioRxiv - Molecular Biology 2020Quote: ... The following primary antibodies were diluted in 1% milk in TBST prior to usage at the indicated concentration: mouse monoclonal α-Tubulin (1:3000, Cell Signaling Technology, #3873), rabbit monoclonal p-p70 S6K (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... and subjected to immunoblotting with the antibodies for phosphorylated Erk-1/2 (Thr202/Tyr 204) or total Erk-1/2 from Cell Signaling Technology (Beverly, MA). Blots were visualized by enhanced chemiluminescence western detection system (Pierce ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were then incubated for 2 hours at room temperature (RT) in antibody binding buffer (PBSTr +1% (v/v) DMSO) before overnight incubation with 1:1000 α-pSmad2 antibody (Cell Signaling Technology #18338) and ...
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bioRxiv - Cancer Biology 2019Quote: ... monoclonal HSP27 (1:1500, catalog number 2402S), MK2 (1:1000, catalog number 3042) and p38 (1:1000, catalog number 9218S) all from Cell Signaling Technology (Danvers, MA); p-HSP27 Ser78 (1:2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1:1,000 for immunoblotting), PGK1 (68540, 1:1000 for immunoblotting), and PKM2 (3198, 1:1000 for immunoblotting) were purchased from Cell Signaling Technology (Danvers, MA). Mouse monoclonal antibodies for VEGF (C-1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sections were then incubated in rabbit polyclonal anti-αSMA or rabbit monoclonal anti-Runx2 at 4° C overnight (Abcam ab5694, 1:100; Cell Signaling Technology, 1:100). Slides were thoroughly washed the next day then incubated in goat anti-rabbit IgG Alexa Fluor 647 secondary antibody for 1 hour at room temperature (ThermoFisher A-21245 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed overnight at 4°C with Rabbit anti-KHSRP (1:1000; Novus) or Rabbit anti-GAPDH (1:2000; Cell Signaling Tech, Beverly, MA), Streptavidin-HRP (1:10000 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed with 1X TBST (3 × 5 min) and then incubated with respective secondary antibodies (Horse anti-mice 1:2000; Horse anti-rabbit 1:2000, (Cell signaling technology, MA, USA) and developed by using ECL chemiluminescent reagent (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... blocked with 5% skimmed milk powder at room temperature for 1 h, the membrane was incubated with the target primary antibody with desired dilution (anti-ACE-2, 1:1000 (Cell signaling technology, MA, USA); TMPRSS2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were probed with the following antibodies at 1/2500 or 1/5000 dilution: rabbit anti-p44/42 MAPK (ERK1/2) antibody (Cell Signaling, cat # 4695 S) to detect ERK2 ...
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bioRxiv - Cell Biology 2021Quote: ... then incubated for 1.5 h at RT with either CD36 (Novus NB400-144, 1:500 dilution) or PPARγ (Cell Signaling 2430S, 1:250 dilution) and GAPDH (Abcam ab181602 ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was washed with 1× TBS for 20 min and then incubated with anti-β-actin primary antibody (1:10000 dilution; Cell Signaling Technology, 3700S) in 1× TBST with 5% milk for 2 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-CEP135, (homemade, raised against a.a. 649-1140, rabbit anti-acetylated-α-Tubulin (1:1,000 IF, 1:500 IHC; Cell Signaling Technologies, cat. No. 5335), rabbit anti-CNTROB (1:1,000 ...
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bioRxiv - Physiology 2021Quote: ... #2217), phosphorylated S6(235/236) (1:1000, #2211), and phosphorylated S6(240/244) (1:1000, #5364) were purchased from Cell Signaling Technology (Danvers, MA). Anti-puromycin (1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... In order to positively identify the proteins, the following antibodies were used: MAN1B1 (SC-100543, 1:500) and actin (1:2000) (Cell Signaling Technology, Beverly, MA). Horse radish peroxidase (HRP)-conjugated secondary antibodies were incubated with the membrane at RT for 1 hr ...
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bioRxiv - Genetics 2019Quote: L4 worms were fed overnight on an NGM plate that had been seeded with 100 μL concentrated OP50 bacteria mixed with 1 μL of 1 mM MitoTracker Deep Red FM dye (Cell Signaling Technology, Cat #8778S).
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... Membranes were then incubated with primary antibody diluted in TBS-T buffer (1:1000 Total OXPHOS Rodent WB Antibody Cocktail [abcam Cat# ab110413] or 1:1000 HSP60 XP Rabbit mAB [Cell Signaling Technology CAT#12165]) overnight at 4°C with gentle rocking ...
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bioRxiv - Microbiology 2022Quote: ... guinea pig polyclonal NSP3 (Lot 55068, 1:2000), VP6 (Lot 53963, 1:2000) antisera or rabbit monoclonal β-actin (8457S, Cell Signaling Technology (CST), 1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-ionized calcium-binding adaptor molecule 1 (Iba-1, FUJIFILM Wako Chemicals USA, Richmond, VA) and anti-glial fibrillary acidic protein (GFAP, Cell Signaling Technology, Danvers, MA) antibodies were diluted to 1:1,000 and used to incubate tissue sections overnight at 4 °C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Membranes were incubated for 1 hour at room temperature with horseradish peroxidase-conjugated horse anti-mouse IgG solution (1:2500, Cell Signaling, Cat. No. 7074P2) diluted in 1% non-fat milk/TBST ...
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bioRxiv - Cell Biology 2024Quote: ... We also used immunofluorescence with Hepatocyte Nuclear Factor 4a (HNF4α) (1/350, PP-H1415- 00, Perseus Proteomics) to define hepatocytes in combination with phospho-histone H3 (pHH3) (9701S, Cell Signaling, diluted 1/200) or Ki67 (12202S ...
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bioRxiv - Cell Biology 2024Quote: ... followed by exposure to primary antibody (anti-Alk1 Rabbit pAb, Abcam #ab68703, 1:500; or, anti-Tubulin Rabbit Ab, Cell Signaling #2144S, 1:500) overnight in 1% BSA TBS-T at 4°C ...
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bioRxiv - Immunology 2024Quote: ... anti-phospho-AMPKα (Thr172) (40H9) (#2535, diluted at 1:1000), anti-AMPKα (#2532, diluted at 1:1000) antibodies from Cell Signaling Technology (Massachusetts, USA), anti-IRF5 (#ab21689 ...
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bioRxiv - Immunology 2023Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with primary anti–SYK (1:1000 dilution, Cell Signaling Technology, no. 2712S), anti–p-p38 (1:1000 dilution ...