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Citations for Cell Signaling Technology :
601 - 650 of 8000 citations for Vacuolar Protein Sorting Associated Protein 26B VPS26B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Protein samples were collected and lysed using a radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysates were quantified by Bradford assay (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was centrifuged at 12 000 g for 15 min and the supernatant with 0.25 mg total protein was incubated with 0.5 μg anti-Ire1α (Cell Signaling) antibody for 4 hours at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg of protein were added to pre-washed 50 μL streptavidin Sepharose bead conjugates (Cell Signaling Technology, 3419S) and rocked overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Equal protein loading of blots was confirmed by immunoblotting of GAPDH (rabbit monoclonal, #2118, Cell Signaling Technology, Danvers, USA), except β-tubulin (mouse monoclonal ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membranes (Prometheus) and probed with phospho-ROS1 Y2274 (3078; 1:1,000; Cell Signaling Technology), ROS1 (D4D6 ...
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bioRxiv - Genomics 2024Quote: ... nuclei was cleared by centrifugation and subjected to pre-clearance by incubating with Protein A agarose beads (Cell Signaling). Pre-cleared cell lysates were separately incubated with CTCF or non-specific IgG antibodies (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: Soluble protein was collected from cell pellets and human brain tissue by resuspension in Cell Lysis Buffer (Cell Signaling Technologies) supplemented with 10µM PMSF on ice ...
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bioRxiv - Physiology 2019Quote: ... Western blotting was performed as previously described (10) and samples were analyzed for protein abundance of FoxO1 (Cell Signaling, #2880), 4-HNE (Abcam ...
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bioRxiv - Systems Biology 2019Quote: ... — 10 mg of digested proteins were dissolved in 1X immunoprecipitation buffer (50 mM MOPS, 10 mM Na2HPO4, 50 mM NaCl, pH 7.2, Cell Signaling). Modified symmetric dimethyl arginine peptides ...
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bioRxiv - Microbiology 2021Quote: Kinase assay was performed using recombinant proteins purified from E.coli and commercially available 10X Kinase buffer (Cell Signaling Technology, #9802). Recombinant proteins (N’-His PINK1 and N’-His Ajuba ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of fusion proteins and 8 μg of FLAG-PKD1-CA were incubated in kinase buffer (Cell Signaling Technology) for 30 min at 30°C in the presence of 200 μM ATP ...
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bioRxiv - Plant Biology 2021Quote: ... 6xHis-tagged ALI1 and 6xHis-tagged control protein were incubated with streptavidin beads (Sepharose® Bead Conjugate, Cell Signaling Technology) that were already loaded with M4 (oxo-C14-HSL tagged with biotin ...
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bioRxiv - Cancer Biology 2022Quote: ... a rabbit mAb raised against synthetic peptide corresponding to residues surrounding Ser518 of human Merlin protein from Cell Signaling Technology Cat # 13281 and a mouse monoclonal antibody ...
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bioRxiv - Plant Biology 2022Quote: ... and probed with anti-phospho-p44/42 mitogen-activated protein kinase (Erk1/2) (Thr202/Tyr204) (Cell Signaling; 1:1,000 dilution), anti-MBP (New England Biolab ...
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bioRxiv - Cell Biology 2022Quote: ... manufacturer and catalog number shown in brackets): Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E, Alexa Fluor 555, 1:200, Cell Signaling, 39855); S6 Ribosomal Protein (54D2 ...
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bioRxiv - Genetics 2023Quote: ... Protein was extracted from frozen tissue by homogenization of tissue with RIPA lysis buffer and PMSF (Cell Signaling, Danvers, MA). Protein concentrations of samples were determined using Bradford Assay (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... anti-Phospho-S6 ribosomal protein (Ser240/244) (pS6) (rabbit polyclonal, #2215, RRID: AB_331682, Cell Signaling Technology; 1:1000 for WB), anti-S6 ribosomal protein (S6 ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were subjected to enrichment of tyrosine phosphorylated proteins using an the PTMScan HS phopshotyrosine kit (Cell Signaling Technology, #38572) following in-solution tryptic digestion and desalting ...
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bioRxiv - Cell Biology 2023Quote: ... Senescence-associated β-Galactosidase (SA β-gal) activity was measured in the hepatocytes using Senescence β-Galactosidase Staining Kit (Cell Signaling Technology-9860, Danvers, MA, USA) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Genetics 2021Quote: ... the samples were incubated with 30 ml of ChIP Grade Protein G magnetic beads (Cat# 9006S; Cell Signaling Technology; Danvers, MA) for 2 hours at 4°C under gentle rotation ...
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bioRxiv - Plant Biology 2022Quote: ... The prey and bait proteins were detected using anti-His (1:1000, No. #2365; Cell Signaling Technology [CST], Danvers, MA, USA) and anti-GST (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Expression of Anti-HA fusion protein was examined by staining cells with rabbit Anti-HA (1:250; #3724, Cell Signaling Technology) and Alexa Fluor@ 488 donkey anti-rabbit IgG (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein accumulation rate upon proteasome inhibition was measured after 3-hour treatment with 10μM of MG-132 (Cell Signaling Technology #2194) in the RPMI medium.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to nitrocellulose using standard methods and membranes were blocked in 5% non-fat dry milk (Cell Signaling Technology) in TBS with 0.2% Tween-20 (Boston BioProducts) ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates for protein analysis were prepared by scraping cells in 1x Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA, USA) containing 1x protease/phosphatase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantification of relative densitometry was obtained by normalizing to the background and to loading control proteins (ACTB, from Cell Signaling Technology) using Image Lab Software (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Molecular Biology 2020Quote: Western blot was performed after chemoenzymatic labeling to detect O-GlcNAcylated proteins using α-streptavidin-HRP (Cell Signaling Technologies, Danvers, MA) (1:3000) ...
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bioRxiv - Biochemistry 2021Quote: ... Primary antibody solution mix contains two primary antibodies raised in two different species (mouse and rabbit) targeting the proteins of interest: rabbit anti-mitofusin 2 (1:50, mAb D2D10, Cell Signaling), rabbit anti-VDAC (1:200 ...
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bioRxiv - Neuroscience 2021Quote: Antibodies for detection of cell surface proteins by western blot of cell lysates were used as provided and were from the following sources: anti-ErbB3 (Cell Signaling, HER3/ErbB3 ...
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bioRxiv - Genetics 2022Quote: ... Membranes were blocked in 4% BSA in 1X TBS and probed with S6 ribosomal protein 1:1000 (2217, Cell Signaling Technologies), phosphor-S6 ribosomal protein 1:1000 (2215 ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Human CDH5 and ICAM-1 protein levels were evaluated by SDS PAGE using rabbit anti-CDH5 (Cell signaling technology Cat# 2500) and mouse anti-ICAM-1 (Santa Cruz biotechnology ...
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bioRxiv - Microbiology 2023Quote: ... ADP-ribosylated proteins were visualised by chemiluminescence using the SignalFire ECL Reagent or the SignalFire Elite ECL Reagent (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Samples used for measuring HMGB1 protein levels in the stool and colonic mucus were homogenized in cell lysis buffer (Cell Signaling) containing complete protease inhibitor (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membranes at 100 volts for 90 minutes in cold 1X transfer buffer (Cell Signaling Technology, 12539S). Membranes were blocked with 5% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were collected and incubated with a 30 μl bed volume of protein-A-sepharose beads and 1.5 μl of α-IGF-1 receptor β (D23H3) XP (Cell Signaling) per sample ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then blotted overnight with anti-human phospho-Akt and anti-human phospho-Erk1/2 mAbs (Cell Signaling, MA, USA), followed by anti-rabbit or anti-mouse horseradish peroxidase(HRP)-conjugated secondary mAb (ThermoFisher scientific ...
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bioRxiv - Molecular Biology 2019Quote: 500 μg of unstimulated HT29 lysate was immunoprecipitated onto Protein A/G coated wells (Pierce #15138) following the kinase assay protocol but with 0.5 μg of IKKα (Cell Signaling Technology #2682) or 0.5 μg of naïve rabbit IgG (Jackson Immunoresearch #011-000-002) ...
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bioRxiv - Microbiology 2020Quote: ... 5 mg of tissue from distal ileal mucosal scrapings was lysed in 250 μL of ice-cold protein lysis buffer (Cell Signaling Technology Cell Lysis Buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The phosphorylated MAPK3 and MAPK6 proteins were detected using polyclonal anti-phospho-p44/42 MAPK (Erk1/2, 1:1000; Cell Signaling Technology), and the detection of the total MAPK3 and 6 protein levels were by using polyclonal anti-MAPK3 (1:1000 ...
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bioRxiv - Biochemistry 2019Quote: ... in tris-buffered saline Tween 20 (TBS-T) for 1 h at RT and then blotted for phosphorylated proteins (Cell Signaling Technology; phospho-EGFR Y1068 #3777 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were immunoprecipitated from precleared lysates overnight at 4°C using 4 μg of control IgG (rabbit or mouse, Cell Signaling Technology) or indicated antibody ...
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bioRxiv - Biophysics 2022Quote: ... 15 μg of total protein was loaded onto an SDS-PAGE (4-12%) to assess LRP6 and β-catenin protein levels using phospho- and total LRP6 (Cell Signaling), active- and total β-catenin (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then prepared for Western blot analysis and total protein was incubated with E-Cadherin (1:1000, Cell Signaling Technology, #3195), Fibronectin (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... Western blot analysis was performed to assess total and phosphorylated p70S6K1 (70kDa ribosomal protein S6 kinase) (T389, Cell Signaling Technology; Boston, MA) as well as 4EBP1 (4E-binding protein 1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Every gel included two molecular-weight markers for separate regions of interest (High Range Color-Coded Prestained Protein Marker; Cell Signaling Technology; and Novex Prestained Protein Standard ...
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bioRxiv - Neuroscience 2023Quote: ... anti-Phospho-p70 ribosomal protein S6 kinase (Thr389) (108D2) (pS6K) (rabbit monoclonal, #9234, RRID: AB_2269803, Cell Signaling Technology; 1:1000 for WB), anti-p70 S6K α (S6K)(mouse monoclonal clone H-9 ...
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bioRxiv - Immunology 2023Quote: ... cycloheximide (CHX) final concentration of 8 ug/mL was used to arrest protein translation and Actinomycin D (Cell Signaling Technology, Cat. 15021) was used to arrest transcription by treating cells with 10 ug/mL Actinomycin D where indicated ...
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bioRxiv - Cancer Biology 2023Quote: ... or EV fraction was loaded alongside a biotinylated ladder and/or pre-stained protein molecular weight markers (Catalog numbers 7727 and 59329, respectively, Cell Signaling Technology) on NuPAGE™ Bis-Tris™ pre-cast gels (ThermoFisher Scientific) ...