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Citations for Cell Signaling Technology :
601 - 650 of 725 citations for 4 4' Bi 7H benz de anthracene 7 7' dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked in 5% BSA with 1% Tween 20 for 1 hour then probed overnight at 4°C with the following primary antibodies at a 1:1,000 dilution: Phospho-STAT1 (Cell Signaling Technologies, 9167S, monoclonal), SOCS1 (Cell Signaling Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked in 5% skimmed milk and incubated overnight at 4°C with primary antibodies for UTX (Cell Signaling Technologies #33510), GFP (JL-8 ...
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bioRxiv - Bioengineering 2023Quote: ... Staining was performed by incubating the cells overnight at 4°C with primary antibodies diluted in 1% BSA targeting α-smooth muscle actin (1:200; 48938S, Cell Signaling Technology), gamma actin (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated overnight at 4°C with the primary antibody mAb c-MYC (dilution 1:1000 in TBST/5% milk; Cell Signaling Technology, D84C12). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated overnight at 4 °C with rabbit anti-c-fos primary antibody (1:500; Cell Signaling Technology, catalog no. 2250, USA) in a permeable buffer containing 2% donkey serum ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated overnight at 4°C with the primary antibody diluted in blocking buffer or SignalStain® Antibody Diluent (cat. 8112, Cell Signaling Technology). Slides were incubated with biotinylated secondary antibodies for 1 hour at room temperature ...
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bioRxiv - Physiology 2024Quote: ... for one hour at room temperature before being incubated overnight at 4°C with the following primary antibodies: anti-phospho-p38 (1:1,000, #9211, Cell Signaling Technology, Billerica, MA); anti-p38 (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for 20 hours with a protein complex of anti-HA antibody (Cell signaling technology, C29F4) and Sera-Mag SpeedBeads Protein A/G (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with anti-HA antibody (Cell Signaling Technology, 1:100). The next day ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid-bound protein was run on 4-20% SDS-PAGE gel and immunoblotted with His-tag antibody (rabbit monoclonal, Cell Signaling Technology, 2365). Blots were visualized on a Li-Cor Odyssey imaging system ...
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bioRxiv - Physiology 2024Quote: ... The following primary antibodies were used to detect respective proteins by incubation overnight at 4°C in 5% BSA: CD44 (#E7k2y, Cell signaling, 1:1000), RHAMM (#E7S4Y ...
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bioRxiv - Bioengineering 2024Quote: ... then incubated at 4 °C overnight in TBST with 5% (w/v) BSA and anti-HLA-G (Cell Signaling catalog #79769, 1:1000) and α/β-tubulin (Cell Signaling catalog #2148 ...
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bioRxiv - Neuroscience 2024Quote: ... All sections were incubated overnight at 4⁰ C with rabbit anti-c-Fos primary antibody (5348, 1:2000; Cell Signaling Technology, RRID: AB_10557109) diluted in 2% NHS in PBS-Tx.
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bioRxiv - Physiology 2024Quote: ... Protein content was measured using DC Assay and samples processed as above to probe for activating transcription 4 (ATF4) abundance (Cell Signaling cat. 11815) as a marker of ER stress.
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bioRxiv - Immunology 2024Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20 μM EDTA + 4 μM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification.
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bioRxiv - Cancer Biology 2024Quote: ... Then 300 µl of lysate was immunoprecipitated by rotating overnight at 4° C with rabbit anti-HA epitope (Cell Signaling #3724, 1:100) for exogenous cyclin B ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked and then incubated overnight at 4°C with the primary antibodies: Tubulin (Sigma, Cat.#T6074; Cell signaling, Cat.#2144), HA-tag (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% BSA in TBS for 1 hr and then incubated overnight at 4° C with anti-pSMAD1/5/8 (1:1000, Cell Signaling Technology, 13820), anti-SMAD1 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The blots were probed with the following primary antibodies and 0.2% Tween20 with overnight incubation at 4°C: anti-p-eIF2α (Abcam; ab32157; 1:1000)(Cell Signaling; 3398s; 1:1000), anti-total-eIF2α (Cell Signaling ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were blocked with 5% milk in PBS-T for 1 h followed by an overnight incubation at 4°C with specific polyclonal primary antibodies anti- RPS6 (1/1000, Cell Signaling Technology, 2217S), anti-RPS6P (1/2000 ...
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bioRxiv - Bioengineering 2024Quote: ... for an hour with rocking at room temperature and were incubated with the following primary antibodies for overnight with rocking at 4 °C: anti-phosphoSRC(Tyr416) (1:1000, Cell Signaling Technology, #2101), anti-p110α (1:1000 ...
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bioRxiv - Biophysics 2024Quote: ... The following primary antibodies were used at 1:1000 dilution at 4 ºC overnight (5% BSA-TBST): Panx1 (91137, Cell Signaling Technologies [CST]), cleaved IL-1β (83186 ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were incubated with primary antibodies overnight at 4°C and then probed with secondary antibodies: horse anti-mouse IgG (Cell Signaling Technology #7076) or goat anti-rabbit IgG (Cell Signaling Technology #7074) ...
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bioRxiv - Cell Biology 2024Quote: ... cardiac myocytes were incubated with primary antibodies in blocking buffer at 4°C overnight as follows: P-S6 Ribosomal ProteinS235/2361:500 (cat# 2211, Cell signaling, Danvers, MA); RyR2 1:500 (cat# MA3-916 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were incubated with primary antibody in TBST overnight at 4°C: Phospho-Akt (Ser473) antibody (Cell Signaling Technology, #9271, 1:1000), Akt (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2022Quote: ... Pulling down of the pre-cleared chromatin was performed with the use of magnetic beads that were previously incubated with 5 µg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) for 8 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Sonicated lysates were cleared once by centrifugation and incubated overnight at 4°C with magnetic beads bound with NFκB antibody (Cell Signaling Technology, Cat. No. 8242s) to enrich for DNA fragments ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were blocked in 5% bovine serum albumin (BSA) and incubated overnight at 4 °C with primary anti-phospho-SYK (Cell Signaling Technologies, 1:1000) or anti-SYK (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The blots were incubated overnight at 4°C with the primary antibodies: His-Tag (D3I1O) XP® Rabbit mAb (1/1,000 dilution) (Cell Signaling Technology, Danvers, MA) and Myc-Tag (9B11 ...
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bioRxiv - Neuroscience 2021Quote: ... was used at dilution 1:20 and incubated with the cell solution at 4°C for 30 min before staining with AlexaFlour 488 goat anti-mouse (Cell Signaling, 1:100 dilution) for another 30 min at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sections were then incubated in rabbit polyclonal anti-αSMA or rabbit monoclonal anti-Runx2 at 4° C overnight (Abcam ab5694, 1:100; Cell Signaling Technology, 1:100). Slides were thoroughly washed the next day then incubated in goat anti-rabbit IgG Alexa Fluor 647 secondary antibody for 1 hour at room temperature (ThermoFisher A-21245 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed overnight at 4°C with Rabbit anti-KHSRP (1:1000; Novus) or Rabbit anti-GAPDH (1:2000; Cell Signaling Tech, Beverly, MA), Streptavidin-HRP (1:10000 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were incubated overnight at 4°C in the primary antibodies against c-Fos (1:6400; catalog #2250S; Cell Signaling Technology, Danvers, MA), and then incubated with the secondary Alexa Fluor® 448 (1:800 ...
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bioRxiv - Cell Biology 2021Quote: ... 50μg of chromatin were immunoprecipitated overnight at 4°C with an antibody against Rev-erb-α (13418S, Cell signaling Technology, Danvers, Massachusetts, United States). BSA/yeast tRNA-blocked Protein A/G dynabeads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... PFA-fixed sections were incubated with the following primary antibodies overnight at 4°C: rabbit anti-cleaved caspase 3 (Cell signaling, #9661, 1:100), mouse anti-BrdU (Dako ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted with blocking solution and incubated with cells overnight at 4°C: Cleaved caspase-3 (rabbit, Cell Signaling #9661, 1:500), Anti-Nestin (mouse ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then rinsed and blocked with 5% BSA for 1hr prior to overnight incubation (4°C) with monoclonal rabbit anti-Phospho-p38 MAPK (Thr180/Tyr182) (D3F9)-XP (Cell signaling, 4511T, 1:1,000) or monoclonal rabbit anti-p38 MAPK (D13E1 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... and subsequently incubated overnight at 4 °C with the primary anti-ADPr-antibody (diluted 1:10’000 in TBS-T; Cell Signaling Technology, Leiden, Netherlands25, 26). Membranes were washed 3x for 5 min with TBS-T and incubated IRDye goat-anti-rabbit IgG secondary antibody (diluted 1:15’000 in TBS-T ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on a 4–12% SDS-PAGE gel followed by standard immunoblot analysis using anti–CISH (Cell Signaling, Clone D4C10, 1:2000) and Vinculin (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were incubated at 4°C overnight with the following mouse-specific primary antibodies: anti-NLRP3 (D4D8T, rabbit monoclonal antibody, 1:1000, #15101, Cell Signaling Technology, Danvers, MA), anti-cleaved Caspase 1 (p20 ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were stained with Ponceau S solution and blocked with 5% nonfat milk in PBS/0.05% Tween 20 for 1 h at RT and subsequently incubated at 4 °C overnight with the following primary antibodies: rabbit anti-MBP (1:1000; Cell Signaling, Danvers, MA, USA), mouse anti-CRYAB (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control antibody (Cell Signaling Technology, 1:300), or anti-IRF1 antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... 300 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control (Cell Signaling Technology, 1:300), anti-Ollas (Novus ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked in 5% milk and incubated overnight at 4 °C in primary antibody (anti-PPARγ, Santa Cruz #sc-7273; or anti-β-actin, Cell Signaling #3700). Washes were performed in TBS-T (20 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were subsequently incubated overnight at 4° C with primary anti-phosphorylated ERK antibody (anti-pERK, Cell Signaling, Cat # 4370, RRID: AB_2315112, 1:400). After primary antibody incubation and PBS wash ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at 4°C overnight in rabbit anti-c-fos(1:1000 dilution, 2250T Rabbit mAb, Cell Signaling Technolog, Danvers, Massachusetts, USA) primary antibody or mouse anti-TPH2 (1:100 dilution ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were then precleared by incubation for 1 hour at 4°C while rotating with 50 μL protein A agarose beads (Cell Signaling Technologies, Cat #9863) CCNT1 antibody (Cell Signaling Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then stained with the appropriate primary antibody diluted in blocking solution and incubated overnight at 4°C: Phospho-Histone H3 (Cell Signaling, #9701, 1:200), Cleaved Caspase 3 (Cell Signaling ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then incubated overnight at 4°C with a variety of primary antibodies including anti-MFN2 (Cell Signaling Technology; diluted 1:200), anti-TRA-1-81 (diluted 1:200 ...