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Citations for Cell Signaling Technology :
601 - 650 of 10000+ citations for 1 Piperidineacetamide 4 2 benzothiazolyl N 4 4 morpholinyl phenyl 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by incubation overnight at 4°C with a specific primary antibody to p-p53ser15 (Cell Signaling, 9284S), β-actin (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858), S6 (Cell Signaling 2217) ...
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bioRxiv - Genetics 2021Quote: ... The following primary antibodies were used at 1:1000 dilution in PBST (0.05% Tween-20) and 5% non-fat dry milk and incubated overnight at 4°C: Myc (Cell Signaling Technology #2276), RBPJ (Cell Signaling Technology #5313) ...
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bioRxiv - Molecular Biology 2021Quote: ... and Tween20 (0.02%)) for 20 minutes at RT followed by treatment overnight at 4 °C with anti-γ-H2AX (Cell Signaling #9718S) diluted 1:500 in Ab mix or anti-PCNA diluted 1:1000 (DAKO #M0879) ...
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bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074; Cell Signaling, Switzerland) and goat anti-mouse antibody (7076 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells grown on chamber microscope slides were fixed in 4% paraformaldehyde and treated with immunofluorescence blocking buffer (catalog #12411, Cell Signaling Technology). Staining with primary antibodies against involucrin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with 100 µl/well of diluted H3K9ac on a shaker at 4°C overnight and then treated with Alexa Fluor 488 conjugate secondary antibody (Cell Signaling #4412) at room temperature ...
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bioRxiv - Genomics 2020Quote: ... The samples were blocked in 10% goat serum and incubated overnight at 4°C with the mouse Cas9 antibody (7A9-3A3 Cell Signaling Technologies) 1:800 dilution in 1% BSA (Supp ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by incubation overnight at 4°C with a specific primary antibody to p-p53ser15 (Cell Signaling, 9284S), β-actin (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: Cells were trypsinized and washed with PBS and then lysed for 30 minutes on a rotating wheel at +4 °C in RIPA buffer supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling, 5872). Lysates were sonicated with a Bioruptor Soni cation System (UCD200 ...
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bioRxiv - Physiology 2021Quote: ... Blots were blocked in 5% bovine serum albumin in tris-buffered saline with 0.1% Tween 20 (TBS-T) and incubated overnight at 4°C with rabbit anti-phospo-Ser505-Akt (Cell Signaling Technology 4054) or mouse anti-α-Tubulin (DSHB 12G10 ...
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bioRxiv - Physiology 2021Quote: ... Sections were blocked with 5% donkey serum in the presence of 0.1% Triton followed by overnight incubation with primary antisera at 4 degrees (pCREB, Cell Signaling; Glucagon, Abcam). After extensive washing ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL of aprotinin and leupeptin] and incubated (16 hrs., 4°C) with either 3 µg non-immune control rabbit IgG (Cell Signaling #272) or with 3 µg of ERRα antibody (Cell Signaling #13826 ...
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bioRxiv - Biochemistry 2022Quote: ... The blots were subsequently incubated overnight at 4°C with primary antibodies against p53 (DO-7 mouse mAb, Cell Signaling, Cat # 48818) and β actin (13E5 rabbit mAb ...
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bioRxiv - Biochemistry 2022Quote: ... 30-50 μg of chromatin was used per sample and incubated with antibodies in a total volume of 600 μL overnight at 4° C with H3K27me3 antibody (Cell Signaling, 9733) or H4K20me0 antibody (Abcam ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... Astrocyte activation to a neurotoxic phenotype was induced in cells at 4-5 DIV by incubation with TNFα (25 ng/ml; Cell Signaling Technology), IL-1α (3 ng/ml ...
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bioRxiv - Neuroscience 2024Quote: ... sheared chromatin was centrifuged at 16,000 x g for 20 minutes at 4 °C and dissolved in 1x ChIP buffer (Cell Signaling Technology, #57976). 2% input was taken and chromatin was incubated with antibodies overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The membranes were probed overnight at 4°C with rabbit monoclonal antibodies against inducible nitric oxide synthase (iNOS; Cell Signaling Technology, 2982) or arginase-1 (Arg-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... and then the supernatant fraction was incubated with anti-BCR-ABL antibody overnight at 4°C and subsequently with protein A magnetic beads (73778, Cell Signaling Technology) for another 4 h at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% skim milk and probed with primary antibodies overnight at 4°C: either rabbit monoclonal anti-TET2 (Cell Signaling Technology) or monoclonal mouse anti-GAPDH (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then cut and incubated with different primary antibodies diluted in 5% milk at 4°C overnight: αH3K27me3 (Cell Signaling C36B11) was used at 1:5000 dilution ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked in 5% non- fat milk or bovine serum albumin (BSA) for 1h and incubated overnight at 4°C with primary antibodies against Cyclin T1 (Cell Signaling Technologies), p24 (Abcam) ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked for 1 hour at room temperature with blocking buffer (Licor) and incubated overnight at 4°C with anti-K48-linked poly-ubiquitin antibody (Cell Signaling Technologies) and anti-β-actin antibody (Santa Cruz Biotechnologies) ...
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bioRxiv - Biophysics 2022Quote: ... 15 μg of total protein was loaded onto an SDS-PAGE (4-12%) to assess LRP6 and β-catenin protein levels using phospho- and total LRP6 (Cell Signaling), active- and total β-catenin (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 20% swine serum in PBS and then incubated with primary antibodies overnight at 4 °C (γH2AX ((#9718, Cell Signaling Technologies). The following day ...
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bioRxiv - Developmental Biology 2022Quote: ... Magnetic beads were allowed to bind with 5 μg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) by incubating for 8 hours at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated nuclei from FT282 and KURAMOCHI were bound to Concalavin A beads and incubated overnight at 4℃ with primary antibody for H3K27me3 (Cell Signaling, RRID:AB_2798370), H3K27ac (Diagenode ...
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bioRxiv - Cancer Biology 2023Quote: ... which were subsequently incubated overnight at 4°C with the following primary antibodies: anti ATP citrate lysase antibody D1X6P (Cell Signaling 13390S), Anti Phospho ATP citrate Lysase antibody Ser455 (Cell Signaling 4331S) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were sorted for the coexpression of each component via fluorescence-activated cell sorting on a FACS ARIA II (Beckton-Dickinson) by staining with appropriate fluorescently tagged anti-Myc and anti-Flag antibody for 30 minutes at 4°C (Cell Signaling Technologies) or expression of the transgenes ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for more than 3 hours with a protein complex of anti-HA antibody (Cell signaling technology) and Dynabeads Protein A (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... which were blocked by 5% non-fat milk (americanBIO, AB10109-01000) and then incubated overnight at 4°C with the following primary antibodies: Nucleocapsid protein (Cell Signaling, #33717), Strep-tagged Nucleocapsid protein (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... and then were blocked with the 10% normal goat serum for 45 minutes at room temperature before incubating with the primary antibodies (anti-γH2AX: Beyotime, no. C2035S-4; anti-pSTING: Cell Signaling Technology, no ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitation was carried out overnight at 4 °C using 5 μl of rabbit polyclonal FosB antibody (2251S, lot 3, Cell Signaling Technology) or 2 μg of rabbit polyclonal IgG isotype (3900S ...
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bioRxiv - Plant Biology 2024Quote: ... overnight at 4 °C and then with horseradish peroxidase (HRP)-conjugated anti-mouse immunoglobulin G (IgG) antibody (7076S; Cell Signaling Technology, USA) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was then incubated under soft agitation overnight at 4°C with the primary antibodies for DNMT1 (Cell Signaling Technology, 5032) and β-actin (Cell Signaling Technology ...
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bioRxiv - Microbiology 2024Quote: ... Samples containing 10 μg of DNA were then incubated overnight at 4°C with either anti-RNA pol II antibody (mouse anti-Rpb1 CTD, Cell Signaling, 2629S), mouse anti-SP1 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2024Quote: ... tissue sections were incubated with 5% goat serum in PBS-T for one hour and then incubated at 4°C overnight with the following antibodies: SOX2 (23064, Cell Signaling Technology), Ki67 (ab16667 ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were fixed in 4% PFA for 10 minutes and processed for immunostaining by standard methods using combinations of rabbit (Cell Signaling, 71D10) or mouse anti-Myc (Cell Signaling ...
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bioRxiv - Genomics 2021Quote: ... Nuclear stain solution was prepared for 4 slides at a time: 20 µLSYTO13 (Nanostring) and 180 µL 1x TBS (Cell Signaling Technologies, 12498S) for a total volume of 200 µL/slide ...
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bioRxiv - Neuroscience 2020Quote: ... were lysed in complete RIP lysis buffer and the cell lysates were divided into equal parts and incubated (overnight with rotation, at 4°C) with anti-Pol II (Cell Signaling Technology (CST), Cat ...
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bioRxiv - Cell Biology 2020Quote: SMC cultured on glass coverslips were fixed in 4% PFA prior to immunostaining with phospho-histone H2AX (γH2AX) antibody (Cell Signaling Technologies #2577) as described previously (19) ...
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bioRxiv - Molecular Biology 2022Quote: ... The blocked membrane was incubated overnight at 4°C with primary antibodies diluted in 1x Tris buffered saline with Tween-20 (TBST, Cell Signaling Technology: 9997S). The membrane was washed twice using TBST ...
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bioRxiv - Systems Biology 2022Quote: ... blocked in 5% dry milk (American bioInc) for 60 min followed by incubation overnight (at 4°C) with primary antibody according to manufacturer’s instruction (Cell Signaling Technology, pSMAD3 (9520S) SMAD3 (9513S) ...
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bioRxiv - Cell Biology 2020Quote: ... of embedded organoids or whole-mounted organoids were incubated overnight at 4°C with rabbit monoclonal anti-p63a antibody (D2K8X, Cell Signaling Technology, MA), polyclonal guinea pig anti-cytokeratin 8/18 (03-GP11 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane was blotted with 5% nonfat milk in TBST at RT for 1h and then incubated with the following antibodies at 4°C overnight: TWIST1(Cell Signaling Technology, 46702S), E-cadherin (Cell Signaling Technology ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... Cells were fixed with 4% paraformaldehyde for 5 min and subsequently permeabilized with 0.2% Triton-X and stained with anti-relB (Cell Signaling Technology, Danvers, USA) followed by goat-anti-rabbit Alexa 546 (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... chemically cross-linked to protein G-agarose beads at 4°C on a mini-rotator. Rabbit anti-GFP (TorryPines, cat. # TP401,) or normal rabbit IgG (Cell signaling, cat. # 2729) chemically cross-linked to protein G agarose beads were used as controls ...
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bioRxiv - Neuroscience 2022Quote: ... and then immunoprecipitated for 3 hours at 4°C by agarose beads preincubated with rabbit anti-HTT D7F7 antibody (Cell Signaling, Cat#5656). To remove the non-specific binding ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated overnight at 4°C in 0.2% BSA/TBS with the following antibodies: anti-Myc mouse IgG (Cell Signaling, cat. No. 2276) for mDGKε-Myc staining and anti-STIM1 rabbit IgG (Cell Signaling ...