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Citations for Cell Signaling Technology :
6351 - 6400 of 10000+ citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Immunoprecipitates were analyzed by Western blot as described above using the following antibodies: anti-HA rabbit polyclonal (1:1000) and anti-Shh rabbit monoclonal (1:1000, Cell signaling).
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Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survivalbioRxiv - Cancer Biology 2022Quote: ... Primary antibodies used: gp-anti-Alk (1:3000) (Loren et al., 2001) and rb anti-tubulin (1:5000; Cell Signaling, #11H10). After washing ...
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bioRxiv - Developmental Biology 2022Quote: ... Discs were incubated with primary antibodies overnight at 4°C: rabbit anti-Cleaved Drosophila cDcp-1 (1:200, Cell Signaling, #9578S), rabbit anti-fkh (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the following primary antibodies overnight: rabbit anti-RAD18 NB100-61063 (Novus) (1:200) and mouse anti-biotin (D5A7) (1:200) (Cell signaling). The rest of the protocol follows Proximity Ligation Assay as above.
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bioRxiv - Cell Biology 2021Quote: ... AMPK and pAMPK were stained using primary antibody polyclonal AMPK (1:1000, 2532, from cell signaling) and anti-phosphorylated AMPK (Thr 172) (1:1000, 2535, Cell Signaling), β-actin (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-chicken 488 (Life Technology A11039 at 1/200) and goat anti-rabbit 488 (Cell Signaling Technology #4412 1/200).
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-HA tag (C29F4) monoclonal antibody (1:1000 for NaV1.6 and 1:2000 for NF186; Cell Signaling, cat. no. 3724); mouse anti-ankyrin G antibody (1:50 ...
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bioRxiv - Pathology 2022Quote: ... Then membranes were incubated with peroxidase-conjugated secondary antibodies for 1 h at room temperature: anti-rabbit immunoglobulin G (IgG) (AB_2099233; Cat.#7074, 1:2000, Cell Signaling Technology), anti-mouse IgG (AB_330924 ...
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bioRxiv - Developmental Biology 2022Quote: Larval wing imaginal discs were stained using the following antibodies: Rabbit anti-cleaved Drosophila Dcp-1 (1:300, Cell Signaling Technology), Rabbit-anti-p-eIF2α (1:300 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were incubated overnight at room temperature (1/100 rat anti-BrdU OBT0030 AbD Serotec; 1/400 rabbit anti-Caspase #9661 Cell Signaling; 1/250 rabbit anti-OTX2 ab92326 Abcam ...
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bioRxiv - Immunology 2019Quote: ... The concentrated supernatant was mixed with SDS loading buffer for western blotting analysis with antibodies for detection of activated caspase-1 (D5782 1:500; Cell Signaling) or mature IL-1β (Asp116 1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... including the primary antibodies (mouse anti-Iba1 antibody, Abcam, 1:400; rabbit anti-iNOS antibody, Abcam, 1:50; mouse anti-GFAP antibody, Cell signaling, 1:400 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and incubated in 5% non-fat dry milk in 1× PBS and 0.1% Tween 20 with the primary antibodies (PHC1 Cell Signaling #13505 1:1000; Rybp Cell Signaling D8J7W 1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Nanobodies were incubated for 1 h at RT and detected using Mouse-anti-Myc antibody (1:1000, 9B11 clone, Cell Signaling). US28 was visualized with the rabbit-anti-US28 antibody (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... The drying product was dissolved in 100 μl PBS and mixed with SDS loading buffer for western blotting analysis with antibodies for detection of activated caspase-1 (D5782 1:500, Cell Signaling), mature IL-1β (Asp116 1:500 ...
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bioRxiv - Microbiology 2019Quote: ... and then incubated for 1 h with 1:1,000 dilution of secondary HRP-conjugated goat anti-rabbit IgG antibody (Cell Signaling Technology). Images of the immunoblots were developed with ECLTM Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... and 0.05% Tween 20] plus 5% BSA before incubation with the primary antibody (1:1000 for mouse monoclonal 12C11 anti-REST and 1:2000 for anti-LaminB1 rabbit polyclonal, Cell Signaling). Anti-rabbit IgG or anti-mouse IgG conjugated to horseradish peroxidase (1:2000 ...
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bioRxiv - Cell Biology 2019Quote: ... which was used at a 1:50 dilution in combination with a secondary anti-mouse HRP antibody (1:10,000, Cell Signaling #7076S). ECL Select (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated goat anti-mouse (1:4000) and goat anti-rabbit (1:4000) secondary antibodies were obtained from Cell Signaling Technology, Danvers ...
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bioRxiv - Cell Biology 2019Quote: ... anti-Mena (1:300, mouse monoclonal, a kind gift from Frank Gertler) and anti-Cre Recombinase (1:300, Cell Signaling 12830). Secondary antibodies used were goat anti-mouse or anti-rabbit IgG conjugated to Alexa Fluor-488 ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies against p-Akt Ser473 (1:100 dilution; 4060) and p-AMPK Thr172 (1:200 dilution; 50081) from Cell Signaling were attempted ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total IkB kinase antibody (p/total IkB 1:1000, 1:500, CST9246S, CST924, Cell Signaling Technology, Danvers, MA), anti-phospho and total panTrk (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... and 1:1000 dilution for c-Myc(c-Myc(9E10) Santacruz) and 1:1000 dilution for both Cek1/Mkc1(9102S Cell Signaling) and 1:1500 dilution for both phospho-Cek1/phosphor-Mkc1(4307T Cell Signaling) ...
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with primary antibody of interest in the blockage solution (c-fos,1:200, Abcam/ab208942; NFκB-p65, 1:200, Cell Signaling Technology/8242 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies include PKM1 and PKM2 (7067S, dilution 1:500, and 4053S, dilution 1:1000, respectively, Cell Signaling Technology, Danvers, MA), p63 and FOXA2 (ab735 and ab108422 ...
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bioRxiv - Physiology 2022Quote: ... GAPDH-HRP (GTX627408-01, 1:120000) and P84 (GTX70220-01, 1:1000) The secondary antibodies were Anti-rabbit IgG (Cell Signaling, 7074 ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies and concentrations were utilized: rabbit anti-Vimentin (Cell Signaling Technology, D21H3, WB 1:1000, IF 1:200), mouse anti-Vimentin (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... The washed cells were then resuspended in 200 μL of PBS containing 1% BSA and 0.1% Triton X-100 (PBSTx/BSA) containing primary antibodies [MCM2 (1:200, D7G11, Cell Signaling Technology) and ψH2AX (1:200 ...
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bioRxiv - Biochemistry 2024Quote: ... Afterwards cells were incubated in blocking solution for 1h at 37 °C and incubated in the primary antibody (Cx36 1:2000, Chemicon, MAB3045; Sec24B 1:1000; Cell Signaling; Grasp55 1:2000 ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-Nwd1 (rabbit polyclonal generated via immunization with the recombinant mouse Nwd1 protein [18] ...
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bioRxiv - Immunology 2023Quote: ... Tissues were then stained with the following fluorophore-conjugated anti-mouse antibodies for (1:100, 1.5 hr, room temperature, gentle agitation) in blocking buffer: Iba-1 (78060S, Cell Signaling Technology), F4/80 (123122 ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-Choline Acetyltransferase (ChAT) (1:500, MilliporeSigma, AB144P, RRID:AB_2079751) and rabbit anti-DARPP-32 (19A3) (1:400, Cell Signaling, 2306, RRID:AB_823479). All secondary antibodies used in this study were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Neuroscience 2023Quote: ... Respective expression was assessed by immunostaining using a mouse anti-HA antibody (1:500; Cell Signaling#2367, RRID AB_10691311 and a rabbit anti-Flag (1:500; Cell Signaling#14793 RRID AB_2572291 followed by Cy5-conjugated donkey anti-mouse (1.9 µg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were then incubated for 1 h with either the rabbit anti-HA antibody (1:500; Cell Signaling #3724, RRID AB_1549585) or the mouse anti-myc antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2 ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies were diluted in 5% blocking solution and membranes were incubated for 1 h at room temperature (1:1000 donkey anti-rabbit IgG, HRP-linked Antibody, #7074S, Cell Signaling). Proteins were detected based on HRP substrate-based enhanced chemiluminescence (#WBKLS0500 ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted onto nitrocellulose membranes, and probed with anti-ZSWIM8 (1:400; Cat #PA5-59492, Thermo) or anti-GAPDH (1:1000; Cat #2118, Cell Signaling) followed by HRP-conjugated anti-rabbit secondary (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... phospho-H2AX (S139) (20E3) rabbit mAb (1:400 for standard immunofluorescence, 1:100 for FFPE tissue staining, Cell Signaling Technology #9718); p21 (12D1 ...
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bioRxiv - Microbiology 2023Quote: ... A competitor solution was prepared by mixing soluble human IgG1-Fc fused Eph receptor/s (150 nM sEphB2-Fc or 1 μM sEphB3-Fc plus 1μM sEphB4-Fc) and anti-myc Alexa 647 (clone 9B11, 1/250 dilution, Cell Signaling Technology) in PBS-BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... each membrane was probed with the appropriate horseradish peroxidase-conjugated secondary antibody (anti-mouse dilution 1:5,000; GTX213112-01, Gene Tex; anti-rabbit dilution 1:3000; 7074S, Cell Signaling Technology), and incubated for 1□h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were labeled with mouse anti-GFP (1:500) antibody and rabbit anti-FOXP1 antibody (Cell Signaling Technology; 1:400) and detected by biotinylated secondary antibody/streptavidin-Alexa Fluor 488 (GFP ...
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bioRxiv - Immunology 2023Quote: ... 0,05% Tween-20 and 1% NGS in PBS) with diluted primary antibodies: (LSD1, 1:200, Cell Signaling Technology, Cat. No. 2184S), (MUC2 ...
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bioRxiv - Genomics 2023Quote: Antibodies against HA-Tag (3724S, 1:200 for immunofluorescence), citrate synthase (14309S, 1:1,000 for Western blot) were from Cell Signaling Technology; SLC25A12 (ab200201 ...
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bioRxiv - Cell Biology 2024Quote: ... WB 1:1000), LC3B (#2775, WB: 1:1000), GABARAPL1 (#26632, WB: 1:1000) and ATG4B (5299, WB :1000) were purchased from Cell Signaling. Beta-actin (A2228 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with horseradish peroxidase-conjugated secondary IgGs (1:1,000 for rabbit #170-6515, mouse #170-6516, Bio-Rad, CA or 1:2,000 for #7076s, Cell signaling, MA), for 1hour at RT ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used: p-PERK (Eli Lilly; 1:50) (73) and PERK (Cell Signaling Technology, 1:200, Cat. #3192).
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bioRxiv - Microbiology 2024Quote: ... Parasites were stained overnight with 13.1 mice monoclonal anti-P28 (1:1000) and rabbit anti-HA (1:250, Cell Signaling Technologies) antibodies ...
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bioRxiv - Neuroscience 2021Quote: Brains were homogenized using Qiagen Tissue-Lyser II (28 Hz, 3 min, 3 times) in lysis buffer (Cell Signaling #9803) containing protease inhibitor cocktail (Roche #4693159001 ...
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bioRxiv - Immunology 2023Quote: ... or fixed for caspase 3 analysis using Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor® 488 Conjugate) (Cell signaling, 9669S). Apoptosis and pyroptosis was assessed by adding Annexin V-FITC (Biolegend ...
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bioRxiv - Cell Biology 2019Quote: ... 1D4, DSHB), mouse anti-Fasciclin III (1:100; 7G10, DSHB) and rabbit anti-phospho-JNK/SAPK (1:100; 81E11 Cell Signaling Technology). Appropriate secondary antibodies conjugated either with Cy3 (1:200 ...