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Citations for Cell Signaling Technology :
551 - 600 of 1336 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... 50 µg total protein was resolved in 8% non-reducing SDS-PAGE gel and blot with PKM2 antibody (Cell Signaling, 3198s).
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bioRxiv - Genetics 2021Quote: ... The blots were incubated overnight at 4 degrees Celcius in a 1:2000 dilution of anti-AUF1 antibody (Cell Signaling D6O4F). The blots were washed (3 × 10 mins ...
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bioRxiv - Synthetic Biology 2019Quote: ... Blots were incubated with primary antibodies (HA-Tag #2367, dilution 1:1000; myc-Tag #2276, dilution 1:1000; Cell Signaling Technology; Vincullin ab129002 ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed in TBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (1:2000; Cell Signaling Tech) diluted in blocking buffer for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were subsequently reblocked and probed with a rabbit monoclonal anti-GAPDH antibody (#2118; 1:5000, Cell Signaling Technology Inc.). The ratios of D3R/GAPDH ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed with PBST for 30 min before incubating with either the fluorescently conjugated secondary antibodies (1:20000) (Li-COR) or horseradish-peroxidase conjugated goat anti-rabbit secondary antibody for the Nrf2 blot (1:5000, #7074) (Cell Signaling) for 1 h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... protein blots were incubated with the following secondary antibodies Anti-Mouse IgG HRP Linked Antibody (Cell Signaling, Cat # 7076S, 1:2500) and Anti-Rabbit IgG HRP Linked Antibody (Cell Signaling ...
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bioRxiv - Pathology 2022Quote: ... blots were transferred to a solution containing anti-rabbit IgG HRP-linked secondary antibody (1:1000 dilution; Cell Signaling Technology 7074S) and incubated for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Samples were lysed in RIPA buffer (Cell Signaling) supplemented with protease inhibitors (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were lysed with lysis buffer (Cell Signaling) and immunoprecipitated with antibodies against Flag (Sigma ...
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bioRxiv - Neuroscience 2020Quote: Cells were harvested in lysis buffer (Cell Signaling) and stored at −80°C until analysis ...
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bioRxiv - Microbiology 2020Quote: ... lysed with 1× lysis buffer (Cell Signaling Technology), and then lysates were centrifugated and incubated with AfRip3-His protein and anti-His-tag mAb-Magnetic Beads for 4 h ...
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bioRxiv - Genetics 2021Quote: ... in 1X RIPA buffer (Cell Signaling, Cat# 9806) supplemented with Complete Mini EDTA-free Protease inhibitor tablets (46548400 ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 1X ChIP buffer (#7008, Cell Signaling) containing PIC ...
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bioRxiv - Cell Biology 2022Quote: Cells were lysed in RIPA buffer (Cell Signaling) and a cocktail of protease inhibitors (Roche ...
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bioRxiv - Immunology 2022Quote: ... and 200μL of Lysis Buffer (Cell Signaling Technologies) supplemented with 1X Halt Protease Inhibitor (ThermoFisher ...
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bioRxiv - Systems Biology 2022Quote: ... Cell lysis buffer (Cell Signaling Technology, Great Britain) was added and incubated for 5 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... sonicated in RIPA lysis buffer (Cell Signaling #9806) supplemented with cOmplete protease inhibitor cocktail (Sigma #11873580001) ...
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bioRxiv - Cell Biology 2024Quote: ... based on commercially available lysis buffer (Cell Signaling) supplemented with benzonase (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended in RIPA buffer (Cell Signaling, 8906) with protease inhibitors (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... cells were lysed in RIPA buffer (Cell Signaling) and mixed with LDS loading buffer (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... kidney, and brain were homogenized at 4°C (Precellys Cryolys Evolution, Bertin Instruments) in lysis buffer (RIPA buffer, 1X, Cell Signaling). Tissue total protein concentration was measured by BCA assay (Pierce BCA) ...
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bioRxiv - Cancer Biology 2020Quote: Human tissue samples and cell preparations were harvested in Complete RIPA Buffer - 1× radio immunoprecipitation buffer (10× RIPA; 9806, Cell Signaling) and one tablet each of PhoStop and Complete Mini (04906845001 and 04693124001 ...
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bioRxiv - Neuroscience 2023Quote: ... and the grey matter was Dounce homogenized in 5 volumes per weight (v/w) buffer containing phosphate buffer saline (PBS) and 1X protease inhibitor (#5871, Cell signaling) in a 15 ml glass homogenizer with 30 up and down strokes at 70% power by hand on ice ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed after RA treatment for 48h with 1X lysis buffer (Cell Lysis Buffer 10 ×, Cell Signaling Technology, Danvers, USA), with protease and phosphatase inhibitors ...
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bioRxiv - Cancer Biology 2021Quote: ... Single clones were picked out after 3 weeks and Cas9 expression level in each clone was determined by western blotting with Cas9 antibody (Cell Signaling, #14697). Cas9 activity was tested using the CRISPRtest™ Functional Cas9 Activity Kit for Human Cells (Cellecta ...
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bioRxiv - Molecular Biology 2021Quote: ... the same amount of protein digestion products was subjected to Western blotting analysis probing with PEP7h antibody (1:5000) or Gapdh-HRP (D16H11, 1:5000, Cell Signaling Technology).
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bioRxiv - Biophysics 2019Quote: ... We also probed for the presence of eRF1 using Western blotting after the ribosome pelleting step (anti-eRF1 antibody from Cell Signaling Technology).
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bioRxiv - Cell Biology 2020Quote: ... Co-immunoprecipitated proteins were eluted from the beads using 150 µl of 1x Laemmli sample buffer for 5 min at +75□C and analysed by western blotting as described above except the HRP-conjugated mouse anti-rabbit native IgG secondary antibody (Cell Signaling, #5127) was used to prevent visualisation of heavy and light chains of co-eluted antibody.
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bioRxiv - Genomics 2022Quote: ... acid-extracted histone lysate was prepared accordingly (Shechter et al., 2007) and subjected to western blotting using anti-H3K9ac (Cell Signaling, 9649), H3K14ac (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... Expression of γ-H2AX was measured by Western blotting using anti-γ-H2AX (Ser139, antibody # 2577; Cell Signaling Technology, Inc., Danvers, MA) as reported by us previously42,44,47.
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bioRxiv - Cell Biology 2019Quote: ... Antibody used in for western-blotting have been previously described (Montagne et al. 2010) or commercially provided for Akt (Cell signaling 4054).
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bioRxiv - Cell Biology 2019Quote: ... samples were transferred to PVDF membranes and western blotting performed as described previously [37] using a primary antibody for phospho-p38 MAPK (#9215; Cell Signaling Technology). Membranes were re-probed with p38α antibody (#9228 ...
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bioRxiv - Microbiology 2019Quote: ... Beads were boiled for 10 minutes at 95 °C and supernatants were subjected to Western blotting for P-Tyr (Cell Signaling 8954).
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bioRxiv - Molecular Biology 2022Quote: ... Proteins captured on the magnetic beads were eluted with 2X SDS loading dye for 5 min and analyzed by Western Blotting using rabbit anti-FLAG (14793S; Cell Signaling Technology) and anti-GFP (A-6455 ...
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bioRxiv - Molecular Biology 2023Quote: ... Pulled-down proteins were visualized by anti-FLAG Western blotting as described above (30, 58) (using anti-FLAG antiserum at 1:1000, #2368S, Cell Signaling Technology). To assess the amounts and sizes of the purified GST proteins used in the pulldowns ...
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bioRxiv - Microbiology 2023Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with antibodies against mouse anti-HA clone 6E2 (Cell Signaling Technology) or rabbit anti-TgSKP1 UOK75 [19].
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bioRxiv - Genomics 2024Quote: ... The extent and kinetics of IKAROS1 degradation was determined by western blotting of cell lysates with an IKZF1 antibody (clone D10E5, Cell Signaling Technology).
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bioRxiv - Cell Biology 2020Quote: ... Transferred blots were blocked for 1 hour in 5% w/v non-fat dry milk in 1X TBST (Cell Signaling Cat# 9997). Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858) ...
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bioRxiv - Biochemistry 2019Quote: ... After washing with TBST blots were then incubated with a 1:1000 dilution of the primary antibody (p-CRAF (S259) (Cell Signaling Technology), p-CRAF (S298/296 ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were stained against β-catenin to determine WNT signaling levels and GAPDH as a loading control (1:1000, Cell signaling #5174S). Secondary horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were saturated and blots were probed with the following primary antibodies: 1:700 rabbit anti-βArr2 (Cell signaling Cat. No. 3857); 1:1000 rabbit anti-GSK3β (Cell signaling Cat ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were then resolved on 10% sodium dodecyl sulfate polyacrylamide gels and blots were probed using the following antibodies: rabbit anti-FLAG (Cell Signaling Technology), rabbit anti-V5 (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... Blots were then incubated in HRP-linked secondary antibody against mouse or rabbit IgG (1:3000, Cell Signaling, Cat# 7074S and 7076S) in 4% BSA for 1 hr at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... blots were incubated overnight at 4°C in the following primary antibodies: β-III-Tubulin (TUJ1, 1:1000; Cell Signaling Technology, 5568), SUFU (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were washed and incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody generated in Rabbit (1:2000; Cell Signaling Technology #7074) for 1.5 hours at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The blots were subsequently incubated overnight at 4°C with primary antibodies against p53 (DO-7 mouse mAb, Cell Signaling, Cat # 48818) and β actin (13E5 rabbit mAb ...
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bioRxiv - Bioengineering 2023Quote: ... The blots were then washed and incubated for 1 h in goat anti-rabbit HRP conjugated secondary antibody (Cell Signaling Technology, 7074) diluted 1:3000 in TBS-T ...
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bioRxiv - Zoology 2021Quote: ... followed by sequential incubations at 4°C in one of the primary antibodies (1:1000 in blocking buffer) and an anti-rabbit HRP-conjugated secondary antibody (1:2000 in blocking buffer (Cell Signaling Technology). Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... and the whole fibrin gel channel was collected and immersed for 10 minutes on ice in 25 µL of cell lysis buffer (fresh stock of 10 mL prepared each time from 1X buffer (9803S, Cell Signaling Technologies) with the addition of 1 µL of Benzonase Nuclease (E8263 ...