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Citations for Cell Signaling Technology :
551 - 600 of 716 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed with 1x TBS-T and then incubated with the secondary anti-mouse (Cat# 7076S, Cell Signaling) or anti-rabbit (Cat# 7074S ...
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bioRxiv - Developmental Biology 2024Quote: ... The membranes were then incubated with anti-mouse immunoglobulin G conjugated to horseradish peroxidase (HRP; 1:5000, Cell Signaling Technology) in 5% milk for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Cell extracts were separated on a 12% Tris-glycine polyacrylamide gel and transferred to a nitrocellulose membrane blotted with phospho-p38 MAPK antibody or p38 MAPK (Cell Signaling) as a loading control ...
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bioRxiv - Immunology 2022Quote: ... Membranes were rinsed and incubated with horseradish peroxidase conjugated secondary antibody (Anti-mouse IgG, HRP-linked Antibody (Cell Signaling Technology, 7076), Anti-rabbit IgG ...
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bioRxiv - Immunology 2020Quote: ... of each sample were resolved by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane and Western blotted with either Aim2 (1:1,000; Cell Signaling #63660) and horseradish peroxidase-conjugated b-actin monoclonal antibody (1:5,000 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 × 10 min with TBST and incubated for 1 h with the appropriate secondary antibody (Cell Signaling Technologies) diluted in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The slides post-PLA were further processed for immunofluorescent staining against the nuclear membrane marker (Lamin A/C, #8617, Cell Signaling). The slides were mounted with Duolink® In Situ Mounting Medium with DAPI (#DUO82040 ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were treated with rabbit anti-V5 antibody at a 1:2000 dilution (Clone D3H8Q, #13202S, Cell Signaling Technology, Danvers, MA) overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... the membrane was incubated overnight with the primary phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, #9101). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to nitrocellulose using standard methods and membranes were blocked in 5% non-fat dry milk (Cell Signaling Technology) in TBS with 0.2% Tween-20 (Boston BioProducts) ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were then incubated for 30 min with a horseradish peroxidase-linked secondary antibody (anti-rabbit or antimouse IgG, Cell Signaling). The Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated overnight at 4°C with the following primary antibody: rabbit anti-IKKα (Cell Signaling 2682; 1:1,000), rabbit anti-phospho-IKKα/β (Ser180/Ser181 ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated with antibody that recognizes proteins IDH 1 (1:1000) and IDH 2 (1:1000) (Cell Signaling, MA) and α-Tubulin (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed overnight at 4°C using primary antibodies (rabbit anti-p S6 (S235/236) 1:1000 (Cell Signaling #4858), rabbit anti-p-S6 (S240/244 ...
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed and then incubated with secondary antibody conjugated with IgG-HRP (cat#7074, Cell Signaling Technology, Boston, MA). Images were captured by ChemiDoc Touch Imaging System (Bio-rad ...
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bioRxiv - Immunology 2020Quote: ... The membranes were then washed and incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies from Cell Signaling Technology and GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were washed with PBS-T and incubated with HRP-coupled goat anti-mouse or anti-rabbit IgG antibodies (Cell Signaling). Detection was performed with Clarity Lightning ECL (Bio Rad ...
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bioRxiv - Cell Biology 2021Quote: ... transferred to nitrocellulose or PVDF membranes and western blotting was conducted using the following primary antibodies: eIF2α (Cell Signaling Technology #9722), p-eIF2α (Cell Signaling Technology #9721) ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies were diluted in 5% blocking solution and membranes were incubated for 1 h at room temperature (1:1000 donkey anti-rabbit IgG, HRP-linked Antibody, #7074S, Cell Signaling). Proteins were detected based on HRP substrate-based enhanced chemiluminescence (#WBKLS0500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with rabbit-anti-human ABCB1 (E1Y7B; mAb #13342; Cell signaling Technology; dilution 1:1000) and mouse-anti-human β-actin (sc-47778 ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted onto nitrocellulose membranes, and probed with anti-ZSWIM8 (1:400; Cat #PA5-59492, Thermo) or anti-GAPDH (1:1000; Cat #2118, Cell Signaling) followed by HRP-conjugated anti-rabbit secondary (1:10,000 ...
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bioRxiv - Physiology 2023Quote: ... non-fat milk in tris-buffered saline (TBS)-0.05% Tween (TBS-T), membranes were incubated overnight with primary antibodies against Drosophila pAkt-Ser505 (1:500, #4054) from Cell Signaling, or Actin5c (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to nitrocellulose membrane (Cytiva) and revealed using following antibodies: rabbit AGR2 (Cell Signaling Technologies, Clone D9V2F, 1/1000), mouse FLAG-M2-HRP (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were then incubated in the same buffer with anti-TAZ and anti-β -actin antibody (#8418, #5125, Cell Signaling) or anti-IHH antibody (#13388-1-AP ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was immunoblotted with primary antibody followed by either Anti-mouse IgG HRP-linked Antibody (Cat # 7076S, Cell Signaling Tech) or Anti-rabbit IgG HRP-linked antibody (Cat #7074S ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with the following primary antibodies in the blocking buffer: rabbit anti-GAPDH (1:2,500; Cell Signaling Technology #2118) and mouse anti-HiBiT (1.0 µg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membranes at 100 volts for 90 minutes in cold 1X transfer buffer (Cell Signaling Technology, 12539S). Membranes were blocked with 5% (w/v ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was incubated with primary antibodies (rabbit anti-EGFR D38B1 Cell Signaling Technologies, rabbit anti-HER2 2242 Cell Signaling Technologies, rabbit anti-PD-L1 E1L3N Cell Signaling Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated with primary antibodies at 4°C overnight: anti-HIF-1α (1:1000, Cat #36169, Cell Signaling Technology, USA) or anti-β-Actin (1:5000 ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was washed 3 times with TBS-T and incubated with horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology #7074) for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... The separated proteins on the gel were transferred onto PVDF membrane and were probed for specific antibodies against Rb (cat# 9309; Cell signaling) phospho-Rb (cat# 8516 ...
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bioRxiv - Biochemistry 2022Quote: ... The membranes were then probed with goat anti-rabbit and goat anti-mouse secondary antibodies (#7074, #7076, Cell Signaling, Danvers, MA), and blots were incubated with Clarity Western ECL Substrate (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was then transferred to a secondary antibody solution containing a 1:5000 dilution of α-mouse HRP (Cell Signaling) for one hour of shaking at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% skim milk and then treated with Trim28 primary and anti–rabbit IgG (Cell Signaling Technology) secondary antibodies ...
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bioRxiv - Genetics 2022Quote: ... Membranes were blocked in 4% BSA in 1X TBS and probed with S6 ribosomal protein 1:1000 (2217, Cell Signaling Technologies), phosphor-S6 ribosomal protein 1:1000 (2215 ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated overnight at 4°C with rabbit anti-phospho-acetyl CoA carboxylase (Ser79) antibody (#3661; Cell Signaling Technology Europe BV ...
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bioRxiv - Physiology 2023Quote: Expression of specific proteins in polysomal profiles (Fig. 6) was determined by probing the membrane with antibodies against RsL7a (dilution 1:4000) from Cell Signaling; 4EBP (dilution 1:4000) ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were reblocked with PBS Blocking Buffer and reprobed overnight at 4°C with anti-Akt antibody (Cell Signaling Technology #9272) diluted 1:1,000 in PBS antibody buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and corresponding polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with various antibodies: rabbit anti-Bcl-2 (1:500; #3498, Cell Signaling), rabbit anti-Bax (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed in PBS and incubated with fluorescent secondary antibody Goat anti-mouse Alexafluor 594 (8890, Cell signaling technology, USA). Membranes were mounted on slides with Fluoromount-G containing DAPI (00-4959-52 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000, CS7076, Cell Signaling, Danvers, MA, USA) and goat anti-rabbit IgG (1:5000 ...
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bioRxiv - Genetics 2023Quote: ... The presence of mitochondrial and cytoplasmic ribosomes in gradient fractions was estimated by incubating resulting membranes with anti-MRPL11 monoclonal antibody (D68F2, Cell Signaling) and anti-RPS6 rabbit monoclonal antibody (Cell Signaling).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the membranes were incubated overnight in a cold room with the following primary antibodies: anti-AKT (1:1000, Cell Signaling #9272S), anti-pAKT (1:1000 ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Membranes were then incubated with rabbit monoclonal anti-CtIP antibodies (1:3000 dilution in TBS-T; #9201, Cell Signaling Technology, USA), rabbit monoclonal anti-cleaved caspase-3 (1:3000 dilution in TBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... were separated on gradient polyacrylamide gels and transferred to nitrocellulose blotting membrane (0.45uM; GE healthcare Primary antibodies against phospho mTOR (Cell signaling), total mTOR (Cell signaling ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were washed for 15 minutes in TBST and incubated with horseradish peroxidase-conjugated anti-mouse IgG (Cell Signaling; cat#: 7076) or IgM (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed three times and incubated with anti-mouse IgG or anti-rabbit IgG HRP-linked antibodies (Cell Signaling Technology) both diluted in PBST (1:2,000) ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed for 10 minutes with PBS-T 3 times and then incubated in DyLight secondary antibodies (Cell Signaling Technologies) for one hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was then washed 3 times in 1x TBST and 1:3000 anti-rabbit secondary antibody (Cell Signaling Technology #7074) in 5% milk TBST was added ...
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bioRxiv - Cancer Biology 2024Quote: ... the membrane was washed in TBS-T and incubated for 1h with goat anti-rabbit (cat. no. 7074S, Cell Signaling Technology) or goat anti-mouse (cat ...