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Citations for Cell Signaling Technology :
551 - 600 of 605 citations for Recombinant Cynomolgus CD3E & CD3D His Fc & Flag Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The antibodies used in this study for immunoblotting (Figure 5) were (1) GST-Tag monoclonal antibody 26H1 (Cell Signaling Technologies catalog number 2624S); (2 ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µl of eluates were loaded on SDS-PAGE gels for western blot analysis using the following antibodies: anti-HA Tag (Cell Signaling Technology, #3724), anti-YWHA (pan ...
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bioRxiv - Cell Biology 2020Quote: ... they were incubated with an HRP-linked secondary antibody (for anti-flag and anti-GFP with Cell Signaling Technology, anti-mouse IgG ...
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bioRxiv - Cell Biology 2022Quote: ... and in primary antibody (α-tRFP, [mKate antibody, Evrogen, AB233], α-FLAG, [Sigma, F1804], α-V5, [Cell Signaling Technologies, 13202S] ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then incubated with rabbit anti-His6 and anti-FLAG antibodies (from Abcam and Cell Signaling Technologies, respectively) at a final concentration of 1:5000 to avoid cross-reactivity with the anti-FLAG-M2 beads ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with a rabbit anti-FLAG M2 antibody (142 ng/mL) (Cell Signaling Technology, Danvers, MA, USA) in 1% BSA–PBS for 1 h at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of MBP-FoxP3ΔN was validated by Western blot using Mouse MBP Tag antibody(8G1) (Cell Signaling Technology, Cat#2396, 1:2000 dilution).
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bioRxiv - Cell Biology 2021Quote: ... 1: 200 for IF) and rabbit anti-Flag mAb (#14793; 1:1000 for WB) were purchased from Cell Signaling Technology. Rabbit anti-Nup133 mAb (#ab155990 ...
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bioRxiv - Genetics 2020Quote: ... The antibodies used for Western Blot and IP-Western Blot analyses included: rabbit anti-FLAG (1:2000, Cell Signaling Technology); rabbit anti-EZH2 (1:1000 Cell Signaling Technology) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of fusion proteins and 8 μg of FLAG-PKD1-CA were incubated in kinase buffer (Cell Signaling Technology) for 30 min at 30°C in the presence of 200 μM ATP ...
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bioRxiv - Microbiology 2022Quote: ... the blot was incubated at 4 °C with primary antibodies (rabbit anti-Flag, Cell Signaling, Cat #: 14793, 1:3,000 dilution) (rabbit anti-Calnexin ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were incubated with a 1:800 dilution of rabbit anti-Flag antibody (2368, Cell Signaling Technology, MA, USA) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: S2R+ cells expressing Flag-dwg were subjected to ChIP assays using SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated in Rabbit anti-FLAG primary antibody (1:400 dilution, Cat #14793, Clone D6W5B, RRID: AB_2572291, Cell Signaling Technology) overnight at 4°C in a humid chamber ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... every sixth section of the LPB was incubated in a primary antibody solution containing rabbit anti HA-Tag (C29F4) monoclonal antibody (1:1000; 3724s, Cell Signaling Technologies, Danvers, MA, USA) overnight followed by incubation in a solution containing a secondary Alexa-Fluor Plus 488 donkey anti-rabbit antibody (1:3000 ...
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bioRxiv - Immunology 2020Quote: ... The lysates were then resolved by SDS-PAGE and immunoblotted with indicated antibodies (Wapl and V5-tag antibodies were purchased from Thermo Fisher Scientific #PA5-38024 and #R960-25, and β-actin antibody was purchased from Cell Signaling Technology #3700).
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bioRxiv - Molecular Biology 2019Quote: ... 1.0% Tween-20) for 1 hour at room temperature and probed with primary antibody (1:1000 HA-Tag Rabbit mAb, Cell Signaling, #3724, in 5% milk) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25; ETV6, Santa Cruz, # sc-166835x; ERG, Cell Signaling Technologies # 97249). Subsequent chromatin capture ...
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bioRxiv - Molecular Biology 2022Quote: ... For immunoprecipitation assays, cell lysates were incubated with Protein G Agarose (cat # 20398, Pierce) and anti-Flag antibody (cat # 2368, Cell Signaling) at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: Cells with stable expression of FLAG-tagged human PDE6D were lysed in 1X cell lysis buffer (#9803, Cell Signaling, Danvers, MA) and incubated with anti-FLAG M2 Affinity Gel (A2220 ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged and myc-tagged proteins were detected with rabbit anti-DYKDDDDK (FLAG) monoclonal antibody and rabbit anti-myc monoclonal antibody (Cell signaling), respectively ...
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bioRxiv - Microbiology 2022Quote: ... After washing 3 times cells were blocked with 2% BSA for 1h at 37 followed by incubation with 1/100 dilution of anti-Flag monoclonal antibody (Cell Signaling) for 1.5h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Respective expression was assessed by immunostaining using a mouse anti-HA antibody (1:500; Cell Signaling#2367, RRID AB_10691311 and a rabbit anti-Flag (1:500; Cell Signaling#14793 RRID AB_2572291 followed by Cy5-conjugated donkey anti-mouse (1.9 µg/ml ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 1h with primary antibodies (1:4,000 dilution, anti-flag mouse -Sigma #F1804 or anti-myc mouse - Cell Signaling #2276). Membranes were then incubated for 1h with a secondary antibody (anti-mouse HRP conjugate at 1:10,000 dilution -Cell Signaling ...
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bioRxiv - Molecular Biology 2023Quote: ... #2639), anti-GAPDH (clone 14C10, #2118), rabbit anti-HA-tag (clone C29F4, #3724) antibodies for immunoblots were purchased from Cell Signaling Technology (Frankfurt am Main, Germany). Mouse anti-β-actin (clone AC-15 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde and processed for immunostaining by standard methods using mouse anti-Flag (9A3, Cell Signaling, 1:400), rabbit anti-Myc (71D10 ...
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bioRxiv - Bioengineering 2021Quote: ... Protein level could also be measured by immunofluorescence using fluorescently labeled antibodies (anti-Flag-Alexa 647, #15009; Cell Signaling, Danvers, MA, USA). These antibodies were flowed into the device and incubated with the immobilized proteins under the ‘button’ for 20 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 3% BSA or 5% milk and probed with the following primary antibodies-1:1000 rabbit anti-Flag (Cell Signaling technology); 1:100 chicken anti-E.histolytica CSN5 (custom) ...
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bioRxiv - Cell Biology 2019Quote: ... Filters were incubated at 4 °C with primary antiserum diluted in 5% milk TBST as follows: FLAG antiserum (1:1000, Cell Signaling Technology), α-tubulin antiserum (1 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were probed with appropriate primary antibodies (anti-FLAG M2 [Sigma-Aldrich], anti-γH2AX, anti-RPA32 [GeneTex], or anti-53BP1 [Cell Signaling]), then washed in PBST (0.05% Tween-20 in PBS) ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were then resolved on 10% sodium dodecyl sulfate polyacrylamide gels and blots were probed using the following antibodies: rabbit anti-FLAG (Cell Signaling Technology), rabbit anti-V5 (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were permeabilized in 0.2% Triton X-100 for 10 min at room temperature following the incubation with anti-FLAG 1:1000 (Cell Signaling Technology, 14793s) and anti-Myc 1:1000 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were permeabilized in 0.2% Triton X-100 for 10 mins at room temperature then incubated with anti-FLAG 1:1000 (Cell Signaling Technology, 14793s) and anti-Myc 1:1000 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed and processed for immunofluorescence as described above with a rabbit ɑ-FLAG antibody (1:1000, Cell Signaling Technologies, D6W5B) to visualize BicD2-KIF fusions ...
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bioRxiv - Cell Biology 2022Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Molecular Biology 2022Quote: ... The remainder of the chromatin solution was incubated with Dynabeads previously coupled with 10 μg ChIP grade antibodies (POGZ, Abcam, ab167408;Flag, Sigma, F1804/F3165; H3K4me3, Abcam, ab1012; H3K27ac, Millipore, MABE647; H3K9me3, Abcam, ab176916; H4K20me3, Cell Signaling Technology, 5737s ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100uM glycine for 30 minutes at room temperature and labeled overnight at 4C with primary antibodies (rabbit anti-FOXP2 Abcam #16046 1:500, rat anti-Flag 1:500 Novus NBP1-06712, mouse anti-NeuN Ab 1:500, Cell Signaling #94403S). For secondary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins captured on the magnetic beads were eluted with 2X SDS loading dye for 5 min and analyzed by Western Blotting using rabbit anti-FLAG (14793S; Cell Signaling Technology) and anti-GFP (A-6455 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were sorted for the coexpression of each component via fluorescence-activated cell sorting on a FACS ARIA II (Beckton-Dickinson) by staining with appropriate fluorescently tagged anti-Myc and anti-Flag antibody for 30 minutes at 4°C (Cell Signaling Technologies) or expression of the transgenes ...
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bioRxiv - Immunology 2023Quote: ... The cells were then suspended in 50 µl antibody buffer pre-mixed with an anti-Flag antibody (1:50; Catalog:14793, Cell signaling Technology) or with anti IgG control (Catalog:2729S ...
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bioRxiv - Immunology 2023Quote: ... Rabbit monoclonal anti-FLAG (D6W5B) antibodies (Cat # 14793S) and Mouse monoclonal anti-MYC (9B11) antibodies (Cat# 2276S) were obtained from Cell Signaling Technology. Mouse polyclonal anti-V5 antibodies were purchased from MBL Life Science (Cat# PM003) ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were resuspended in 1ml total volume cold Co-IP Buffer containing rabbit IgG or rabbit anti-FLAG antibody (Cell Signaling #14793S), 4μg per sample ...
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bioRxiv - Microbiology 2024Quote: ... and 5% non-fat milk for 60 minutes at room temperature and incubated with primary monoclonal antibody (1:1000 rabbit anti-FLAG, anti-HA, Cell Signaling Technologies) overnight at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit monoclonal anti-CREB1 1:2,000 diluted in the corresponding blocking solution (5% BSA-TBST) (Cat# 9197, RRID: AB_331277, immunogen: recombinant protein specific to the amino terminus of human CREB1 protein, Cell Signaling Technology, Danvers, MA, USA); rabbit affinity isolated anti-actin 1:3,500 diluted in PBST (Cat# A2066 ...
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bioRxiv - Neuroscience 2023Quote: ... penicillin/streptomycin and 2% Fetal bovine serum (for vehicle group) or the same media with 100ng/ml recombinant colony-stimulating factor 1 (Csf1, Cell Signaling, Boston, MA, USA). Csf1 stock was diluted in saline.
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bioRxiv - Cell Biology 2024Quote: ... An equal amount of monosomes per sample was incubated overnight with 100 µl of anti-FLAG Sepharose beads (Cell signaling Technology, Massachusetts, USA) at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was incubated with 1 µg/ml primary antibody (rabbit polyclonal anti-RAD21, abcam ab154769, rabbit polyclonal anti-WAPL, Proteintech 16370-1-AP, rabbit polyclonal anti-CTCF, Cell Signaling Technologies #2899S, rabbit anti-PK-tag (V5), abcam ab15828 ...
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bioRxiv - Developmental Biology 2019Quote: ... resuspended in 250μl of blocking buffer and respective antibodies were added (1μg/μl Rabbit anti-IgG Millipore – CA92590; 1μg/μl Rabbit anti-H3K27ac Abcam – ab4729; 2.5μg Rabbit anti-H3K27me3, Cell signaling – C36B11; 3μg anti-Flag, Sigma – F3165). Antibody and beads were incubated overnight rotating at 4°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the cell lysate and the FLAG-immunoprecipitated protein complexes were separated on a 66-440 kDa Separation Matrix (Proteinsimple, CAT# SM-W008) and detected using anti-FLAG (Cell Signaling Technology, D6W5B, CAT# 14793S) and anti-BRCA2 (Cell Signaling Technology ...