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Citations for Cell Signaling Technology :
501 - 550 of 952 citations for Total Phenols Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: The following antibodies were used in the immunostaining assays: Alexa Fluor® 488 Phalloidin (Cat# 8878, Cell Signaling, 1:200), anti-Myosin (Cat# A4.1025 ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysate was obtained by incubating treated and untreated DLD-1 cells in 1X RIPA (Cell Signaling Tech, Beverly MA), with phosphatase inhibitor cocktail and protease inhibitor tablets (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 µg total protein was resolved in 8% non-reducing SDS-PAGE gel and blot with PKM2 antibody (Cell Signaling, 3198s).
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bioRxiv - Developmental Biology 2021Quote: ... The bead-immobilized GTP-bound RhoA and total RhoA in the lysates were probed by immunoblotting with anti-RhoA antibody (Cell Signaling).
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight with primary rabbit antibodies raised against total or active β-Catenin (Cell Signaling Technology, #8480 and #8814 respectively), and primary mouse antibodies raised against α-tubulin (Santa Cruz Biotechnology #sc-5286 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then washed three times with PBS and total cells were labeled with a 1:1,000 dilution of DRAQ5 (Cell Signaling Technology) for 5 min at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... For primary antibodies against [p] ERK (diluted 1:500) and total ERK (tERK; diluted 1:500; Cell Signaling Technology, Danvers, MA), antibodies were diluted in 0.1% TBST with 5% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was incubated with rabbit monoclonal antibody against total ERK (Ref: #4695) or rabbit monoclonal antibody against phospho-ERK (Ref: #4370) both from Cell Signaling, and then with Amersham ECL Rabbit IgG ...
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bioRxiv - Systems Biology 2022Quote: ... cells were lysed in RIPA buffer and analyzed by SDS-PAGE followed by western blotting with antibodies to phospho and total Smad3 (Cell Signaling). The blots were quantified using an Odyssey fluorescent imaging system.
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bioRxiv - Neuroscience 2020Quote: ... One equal volume was kept for total extracts and the rest was incubated with antibody against mGlu5 (rabbit clone D6E7B from Cell Signaling) with rotation overnight at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... and bound Myc bead (1/2 total amount) samples were analyzed by SDS-PAGE and immunoblotting with rabbit anti-Myc monoclonal antibody (1:2000; Cell Signaling) to detect Myc-tagged EXOSC5 and EXOSC2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... MCF7 cell lysate as prepared above was diluted to 0.8 mg/mL total protein in 1:3 lysis buffer:dilution buffer with 0.5 nM rabbit anti-BRD4 antibody (Cell Signaling Technology; E2A7X) and 1 nM CoraFluor-1-labeled anti-rabbit nano secondary ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then washed three times with PBS and total cells were labeled with a 1:1,000 dilution of DRAQ5 (Cell Signaling Technology) for 5 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... NFκB phospho (rabbit-anti-NFκB p65 total, Cell Signaling Technology #8242; mouse anti-NFκB p65 p-Ser536, Cell Signaling Technology #13346), mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT ...
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bioRxiv - Microbiology 2022Quote: ... The cells were harvested at 48 h pt and equal amounts of proteins were analyzed by western blot for p-eIF2α or total eIF2α (both antibodies were obtained from Cell Signaling). Expression of E was detected using an HA-tag antibody (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... aliquots of total cell lysate were subjected to SDS-PAGE and Western blot analysis with anti-phosphotyrosine (1:3000, Cell signaling) antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were stained for β-galactosidase using the activity kit (Kit 9860S) from Cell Signaling Technologies according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The pull-down assay of ZFP982-MYC tagged protein was performed through co-Immunoprecipitation (co-IP) using MYC-Tag (9B11) antibody (#2276, Cell Signaling). For MYC-co-IP ...
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bioRxiv - Immunology 2021Quote: ... anti-β-actin (3700) and the secondary antibodies used for western blot and immunofluorescence assay were purchased from Cell Signaling Technology. Anti-SYVN1 (ab170901 ...
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bioRxiv - Neuroscience 2021Quote: Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... For immunoprecipitation assays, cell lysates were incubated with Protein G Agarose (cat # 20398, Pierce) and anti-Flag antibody (cat # 2368, Cell Signaling) at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... were used in immunofluorescence staining and Western blot assays: Ki-67 (AB_2797703) (mouse mAb, Cell Signaling Technology, Cat# 9449, 1:800), p-S6S235/S236 (AB_10695457 ...
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bioRxiv - Synthetic Biology 2024Quote: Antibodies used for live cell flow cytometry assays include Alexa Fluor 647-Anti-Myc tag conjugated antibody (Cell Signaling Technologies #2233S) used at 1:100 ...
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bioRxiv - Immunology 2024Quote: ... In vitro ADC cytotoxicity against these cell lines was assessed using a 72-hour XTT viability assay per the manufacturer’s recommendations (Cell Signaling Technology) and as described12.
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bioRxiv - Molecular Biology 2020Quote: ... Blots were washed three times (total 30 min) and incubated with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technologies) for 1 h at room temperature with gentle rocking ...
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bioRxiv - Immunology 2020Quote: ... p-Akt (Ser473) (Clone D9E) and total Akt (clone 4604) and c-Myc antibody (Clone 9E10) were obtained from Cell Signaling Technology. Alfa-tubulin antibody was purchased from Abcam (Clone DM1A) ...
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bioRxiv - Biophysics 2022Quote: ... Cellular protein tyrosine phosphorylation was performed by immunoblotting of total cell lysate (20 μg) using anti-pTyr antibody (4G10, a gift form Prof. Mangeat; CRBM) and anti-Actin (Cell Signaling Technology). Signal quantification and analyses were performed using ImageJ and GraphPad Prism respectively ...
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bioRxiv - Immunology 2022Quote: ... intracellular immunostaining for total phospho-Tyrosine was performed by incubating with phospho-tyrosine mAb (P-Tyr-100 Alexa Fluor 647, Cell Signaling Technology). Cells were washed twice with and resuspended in FACS buffer and analyzed using a BD FACS Canto II flow cytometer (BD Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... p44/42 MAPK (Total Erk) (4695S) and phospho-p44/42 MAPK (p-Erk)(Thr202/Tyr204) (4370S) were obtained from Cell Signaling Technologies. β-Actin (SC-47778 HRP ...
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bioRxiv - Neuroscience 2020Quote: ... 20 µg of total brain protein were loaded and anti-GFAP antibody (D1F4Q) XP Rabbit mAb (1:3000; Cell Signaling Technology, 12389), anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cat no.: 4370; Dilution 1:1000) and total p44/42 MAPK (Cat no.: 4695; Dilution 1:1000) were obtained from Cell Signaling Technology. Horseradish peroxidase (HRP)-conjugated anti-rabbit (Cat no. ...
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bioRxiv - Biophysics 2022Quote: ... 15 μg of total protein was loaded onto an SDS-PAGE (4-12%) to assess LRP6 and β-catenin protein levels using phospho- and total LRP6 (Cell Signaling), active- and total β-catenin (Cell Signaling ...
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bioRxiv - Biochemistry 2022Quote: ... The total p53 and its phosphorylation at Thr55 were detected by anti-p53 antibody (Cell signaling Technology, 1C12, Cat#: 2524, 1:1000) and anti-p53 pThr55 antibody (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... 30-50 μg of chromatin was used per sample and incubated with antibodies in a total volume of 600 μL overnight at 4° C with H3K27me3 antibody (Cell Signaling, 9733) or H4K20me0 antibody (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then prepared for Western blot analysis and total protein was incubated with E-Cadherin (1:1000, Cell Signaling Technology, #3195), Fibronectin (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... Western blot analysis was performed to assess total and phosphorylated p70S6K1 (70kDa ribosomal protein S6 kinase) (T389, Cell Signaling Technology; Boston, MA) as well as 4EBP1 (4E-binding protein 1 ...
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MIIP downregulation promotes colorectal cancer progression via inducing adjacent adipocytes browningbioRxiv - Cancer Biology 2023Quote: ... Protein lysates containing 1 mg total protein were precleaned and precipitated with indicated antibody or IgG (Cell Signaling Technology, Danvers, MA, US) for 6 h at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were combined with QD-fibril complexes in a total volume of 200 μL with 1% milk and 1:1000 mouse anti-Myc antibody (Cell Signaling, 2276S) and allowed to incubate with end-over-end mixing at room temperature for approximately 3 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were resuspended in 1ml total volume cold Co-IP Buffer containing rabbit IgG or rabbit anti-FLAG antibody (Cell Signaling #14793S), 4μg per sample ...
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bioRxiv - Bioengineering 2023Quote: ... 45 µL of cells suspension were incubated for 90 min at RT in a total volume of 55 µL with 10 nM BioGCSFR and 1:200 Myc-Tag (9B11) Mouse mAb (2276, Cell Signaling Technology). After one wash with 500 µL PBS ...
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bioRxiv - Systems Biology 2023Quote: ... followed by overnight incubation at room temperature with the primary antibody (ThermoFisher SolA15 for KI67, Cell Signaling Technology 7F5 for total p53). The following morning ...
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bioRxiv - Molecular Biology 2023Quote: ... Then were processed with commercial SA-β-galactosidase staining kits following provider instructions (Kit #9860 from Cell signaling).
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bioRxiv - Cancer Biology 2022Quote: ... EMT kit (Cell Signaling Technology, 1:2000), beta-actin (Santa Cruz Biotech,1:4000) ...
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bioRxiv - Immunology 2023Quote: ... with the SimpleChIP Plus kit (Cell Signaling). Sequencing libraries were prepared from isolated plasmids as for MPRA and sequencing on a MiSeq (50bp ...
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bioRxiv - Immunology 2023Quote: ... with the SimpleChIP Plus kit (Cell Signaling). The ratio of rs2836882 alleles in the PU.1-bound DNA was quantified in duplicate by TaqMan genotyping (assay C 2601507_20) ...
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bioRxiv - Immunology 2024Quote: ... using an immunofluorescence kit (Cell Signaling Technologies). Samples were stained with DAPI (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... 80 μg of total cell lysates were analyzed by Western blot using the following antibodies and concentrations: LRP6 (1:1000, Cell Signaling Technology 3395), LRP5 (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: Co-immunoprecipitation of endogenous PARP1 and SET8 were performed with 200 µg of total extract from crosslinked (1% formaldehyde for 10 min) HCT116 or HeLa cells using anti-PARP1 antibody (Cell Signaling Technology # 9532), anti-SET8 antibody (Santa Cruz Biotechnology # sc-515433 ...
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bioRxiv - Developmental Biology 2021Quote: Protein lysates from 28 hpf control and ncl-/- zebrafish were used (n = 25 per biological replicate; total three biological replicates) for pre-conjugation with Mdm2 antibody (Cell Signaling Technology, #86934) and IgG with magnetic beads ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were later stripped by incubating in 0.5M NaOH for 10 min and then re-probed for total Akt levels by incubating with anti-Akt (C67E7) antibody (1:1000 dilution, Cell Signaling Technology 4691T). The density of each immunoreactive phospho-protein band was expressed as a fraction of the band for the total AKT protein in the same lane.