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Citations for Cell Signaling Technology :
5201 - 5250 of 10000+ citations for Human Breast Cancer Metastasis Suppressor 1 BRMS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... #9188),Ki-67 (1:1,000 dilution; Novocastra; NCL-KI-67-P) and Cleaved Caspase 3 (1:200 dilution; Cell Signaling, #9661). p-CREB 1:100 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... The washed cells were then resuspended in 200 μL of PBS containing 1% BSA and 0.1% Triton X-100 (PBSTx/BSA) containing primary antibodies [MCM2 (1:200, D7G11, Cell Signaling Technology) and ψH2AX (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with 5% non-fat dry milk in TBST and incubated with primary antibodies against Perilipin-2 (1:5000 gift from Dr. Ronal Kuhnlein) 13 and Actin (1:1000, Cell Signaling). The western blot images were captured by a BIO-RAD workstation.
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bioRxiv - Molecular Biology 2024Quote: ... anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-Nwd1 (rabbit polyclonal generated via immunization with the recombinant mouse Nwd1 protein [18] ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies include PKM1 and PKM2 (7067S, dilution 1:500, and 4053S, dilution 1:1000, respectively, Cell Signaling Technology, Danvers, MA), p63 and FOXA2 (ab735 and ab108422 ...
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bioRxiv - Cell Biology 2024Quote: ... following incubation with the primary antibodies anti-p53 (1:500, mouse, (Ab-6) Pantropic OP43 Sigma-Aldrich) and anti-GAPDH (1:1000, rabbit, 14C10 Cell Signaling) for 1h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Afterwards cells were incubated in blocking solution for 1h at 37 °C and incubated in the primary antibody (Cx36 1:2000, Chemicon, MAB3045; Sec24B 1:1000; Cell Signaling; Grasp55 1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... Respective expression was assessed by immunostaining using a mouse anti-HA antibody (1:500; Cell Signaling#2367, RRID AB_10691311 and a rabbit anti-Flag (1:500; Cell Signaling#14793 RRID AB_2572291 followed by Cy5-conjugated donkey anti-mouse (1.9 µg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were then incubated for 1 h with either the rabbit anti-HA antibody (1:500; Cell Signaling #3724, RRID AB_1549585) or the mouse anti-myc antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2 ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies were diluted in 5% blocking solution and membranes were incubated for 1 h at room temperature (1:1000 donkey anti-rabbit IgG, HRP-linked Antibody, #7074S, Cell Signaling). Proteins were detected based on HRP substrate-based enhanced chemiluminescence (#WBKLS0500 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with PBS-0.1% Tween 20 (PBS-T) containing 5% milk and were incubated with rabbit monoclonal anti-CYLD (1:500, #8462 Cell Signaling) and mouse monoclonal anti-β-actin (1:5,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted onto nitrocellulose membranes, and probed with anti-ZSWIM8 (1:400; Cat #PA5-59492, Thermo) or anti-GAPDH (1:1000; Cat #2118, Cell Signaling) followed by HRP-conjugated anti-rabbit secondary (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... phospho-H2AX (S139) (20E3) rabbit mAb (1:400 for standard immunofluorescence, 1:100 for FFPE tissue staining, Cell Signaling Technology #9718); p21 (12D1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking solution was replaced with 1° antibody (Ab) (Phospho-Smad1/5/9 Rabbit mAb #13820, Cell Signaling Technology 13820T, 1:300) in 10%NGS/PBS-Tx and the slides were incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000, 71G4B7, Cell Signaling; beta actin 1:5000 sc-4778 ...
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bioRxiv - Microbiology 2023Quote: ... 3% BSA overnight at 4 □C (rabbit α-LC3B 1:1000 – Cell Signaling/ #4108, rabbit α-Atg5 1:1000 – Cell Signaling/#2630 ...
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bioRxiv - Microbiology 2023Quote: ... A competitor solution was prepared by mixing soluble human IgG1-Fc fused Eph receptor/s (150 nM sEphB2-Fc or 1 μM sEphB3-Fc plus 1μM sEphB4-Fc) and anti-myc Alexa 647 (clone 9B11, 1/250 dilution, Cell Signaling Technology) in PBS-BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... each membrane was probed with the appropriate horseradish peroxidase-conjugated secondary antibody (anti-mouse dilution 1:5,000; GTX213112-01, Gene Tex; anti-rabbit dilution 1:3000; 7074S, Cell Signaling Technology), and incubated for 1□h at room temperature ...
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bioRxiv - Genomics 2023Quote: Antibodies against HA-Tag (3724S, 1:200 for immunofluorescence), citrate synthase (14309S, 1:1,000 for Western blot) were from Cell Signaling Technology; SLC25A12 (ab200201 ...
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bioRxiv - Immunology 2023Quote: ... Tissues were then stained with the following fluorophore-conjugated anti-mouse antibodies for (1:100, 1.5 hr, room temperature, gentle agitation) in blocking buffer: Iba-1 (78060S, Cell Signaling Technology), F4/80 (123122 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were labeled with mouse anti-GFP (1:500) antibody and rabbit anti-FOXP1 antibody (Cell Signaling Technology; 1:400) and detected by biotinylated secondary antibody/streptavidin-Alexa Fluor 488 (GFP ...
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bioRxiv - Immunology 2023Quote: ... 0,05% Tween-20 and 1% NGS in PBS) with diluted primary antibodies: (LSD1, 1:200, Cell Signaling Technology, Cat. No. 2184S), (MUC2 ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-Choline Acetyltransferase (ChAT) (1:500, MilliporeSigma, AB144P, RRID:AB_2079751) and rabbit anti-DARPP-32 (19A3) (1:400, Cell Signaling, 2306, RRID:AB_823479). All secondary antibodies used in this study were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used: p-PERK (Eli Lilly; 1:50) (73) and PERK (Cell Signaling Technology, 1:200, Cat. #3192).
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bioRxiv - Neuroscience 2024Quote: ... and incubated with horseradish peroxidase-conjugated secondary IgGs (1:1,000 for rabbit #170-6515, mouse #170-6516, Bio-Rad, CA or 1:2,000 for #7076s, Cell signaling, MA), for 1hour at RT ...
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bioRxiv - Cell Biology 2024Quote: ... WB 1:1000), LC3B (#2775, WB: 1:1000), GABARAPL1 (#26632, WB: 1:1000) and ATG4B (5299, WB :1000) were purchased from Cell Signaling. Beta-actin (A2228 ...
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bioRxiv - Microbiology 2024Quote: ... Parasites were stained overnight with 13.1 mice monoclonal anti-P28 (1:1000) and rabbit anti-HA (1:250, Cell Signaling Technologies) antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... 1D4, DSHB), mouse anti-Fasciclin III (1:100; 7G10, DSHB) and rabbit anti-phospho-JNK/SAPK (1:100; 81E11 Cell Signaling Technology). Appropriate secondary antibodies conjugated either with Cy3 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... The Developmental Studies Hybridoma Bank at the University of Iowa) and Rabbit anti-cleaved Drosophila Dcp-1 (Asp216) (1:100; Cell Signaling Technologies). Secondary antibodies were used at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... The primary antibody against phosphorylated RSKS-1 is anti-phospho-p70 S6 kinase (Thr389) (1A5) Mouse mAb (Cell Signaling, #9206, 1:1,000), which detects the conserved residue Thr404 in worms and was tested for its specificity using wildtype and rsks-1 deletion mutant lysates ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated with the following primary antibodies for 1 hour at RT: rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S), mouse anti-SMA 1:300 (Sigma A5228 ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were washed with chilled FACS buffer (DPBS with 1% FBS and 2mM EDTA) and then incubated with the mouse anti-SSEA4 antibody (1: 50; 4755S, Cell Signaling Technology) on ice for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 hour at room temperature before transferred to the anti-pH3 antibody solution (1:200 in blocking solution; #9706, Cell Signaling; RRID:AB_331748) at 4°C for 3 days ...
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bioRxiv - Molecular Biology 2021Quote: ... the same amount of protein digestion products was subjected to Western blotting analysis probing with PEP7h antibody (1:5000) or Gapdh-HRP (D16H11, 1:5000, Cell Signaling Technology).
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bioRxiv - Neuroscience 2019Quote: ... 11A5(Anderson et al., 2006) (mouse monoclonal 11A5 kindly provided by Imago Pharmaceuticals, 1:25,000) and D1R1R (rabbit mAb #20706S, Cell signaling. 1:1000), NF-L (mouse mAb #2835 ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit polyclonal SDHA (#11998, 1:1000) and rabbit monoclonal COXIV (3E11 clone, #4850, 1:1000) antibodies were purchased from Cell Signaling Technology. The donkey anti-rabbit (NA934 ...
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bioRxiv - Cell Biology 2019Quote: ... 1:100 for IF) and Rabbit anti-GFP (#2956, 1:100 for IF and 1:1000 for WB, respectively) were from Cell Signaling Technology.
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bioRxiv - Cell Biology 2020Quote: ... WB 1:1000), rabbit anti-TYRO3 (5585, WB 1:1000), rabbit anti-phospho-AXL (Tyr702) (5724, WB 1:1000) all from Cell Signaling Technology; mouse anti-α-tubulin (T5168 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-ERK1/2 (dilution 1:2000) and rabbit α-tubulin (dilution 1:2000) all purchased from Cell Signaling Technology (Danvers, MA). The phospho-ERK1/2 expression was evaluated first using a 680-nm secondary anti-mouse antibody ...
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bioRxiv - Cell Biology 2020Quote: ... Protein separation was achieved via SDS-PAGE and proteins were transferred to nitrocellulose for immunoblotting with the following primary antibody dilutions: rabbit monoclonal anti–Caveolin-1 (1:1000; Cell Signaling #3267), mouse monoclonal anti–Actin (1:1000 ...
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bioRxiv - Biophysics 2021Quote: ... and stained with 1:50 RBD-sfGFP expression medium (prepared as previously described and 1:250 anti-myc Alexa 647 (clone 9B11, Cell Signaling Technology) in PBS-BSA ...
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bioRxiv - Bioengineering 2020Quote: ... and blocked with 1% BSA for 1 hour prior to incubation with primary antibodies raised against OCT4 (Cell Signaling, Cat. # 28901:800), NANOG (Cell Signaling ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained with APC-conjugated CD45 (1:25 dilution; Miltenyi) and Alexa Fluor 488-conjugated pan-keratin (1:25 dilution, Cell Signaling Technology) antibodies for 10 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mCherry (AB0081; 1:300; SICGEN) for neuronal morphology and anti-phospho-Iϰβ-α (14D4 rabbit monoclonal antibody; 1:500; Cell Signaling) for labeling the AIS ...
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bioRxiv - Cell Biology 2021Quote: ... and Cleaved Caspase 3 (#9664, 1:400 dilution for IF) and anti-Rsk2 (#5528; 1:200 dilution for IF) were purchased from Cell Signaling Technology. Anti-Tubulin α (66031-1-Ig ...
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bioRxiv - Zoology 2021Quote: ... followed by sequential incubations at 4°C in one of the primary antibodies (1:1000 in blocking buffer) and an anti-rabbit HRP-conjugated secondary antibody (1:2000 in blocking buffer (Cell Signaling Technology). Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... 1:1,000, 9251), anti-c-jun (60A8, 1:1,000, 9165), anti-phospho-c-jun (S63, 1:1,000, 9261) (Cell Signaling, Beverly, MA, USA); anti-BECN1/Beclin-1 (1:1,000 ...
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bioRxiv - Microbiology 2019Quote: ... 1:3000 diluted in 1% BSA) and mouse monoclonal anti- HA (6E2) tag antibody (1:1000 dilution in 3% bovine serum albumin, Cell signaling, #2367) for 1hr at RT ...