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Citations for Cell Signaling Technology :
5151 - 5200 of 10000+ citations for Mouse Anti Clostridium Difficile Toxin B Antibody TB7 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The membranes were then incubated overnight at 4 °C with either rabbit anti-p-MEK antibody against Ser217/Ser221 (1:1000) (Cell Signaling, #9121), rabbit anti-p-ERK antibody against Thr202/Tyr204 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... The final concentrations of the primary and secondary antibodies and blocking solutions used in the brain sections are as follows: Rabbit anti-cFos (Cell signaling 2250S), donkey anti-rabbit 647 (Jackson ImmunoResearch 711-605-152 ...
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bioRxiv - Neuroscience 2022Quote: ... slide mounted sections were incubated overnight at room temperature with a rabbit anti-cFos monoclonal primary antibody (9F6, Cell Signaling, 1:8000), diluted in 0.1M phosphate buffer at pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were further incubated with α-Syn rabbit polyclonal antibody 1:500 (US biological) followed by goat anti-rabbit secondary at 1:1000 (Cell signaling) after which treated with TMB substrate ...
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bioRxiv - Cancer Biology 2022Quote: ... Expression of γ-H2AX was measured by Western blotting using anti-γ-H2AX (Ser139, antibody # 2577; Cell Signaling Technology, Inc., Danvers, MA) as reported by us previously42,44,47.
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bioRxiv - Molecular Biology 2022Quote: ... the same amount of each cellular lysate was analyzed by gel electrophoresis and western blot using anti-WDR5 antibody (D9E1I; Cell Signaling #13105), anti-HSP90 antibody (F-8 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Immunofluorescent staining was performed as previously described (Ibrahim et al 2019) and the following antibodies were used: rabbit anti-insulin (C27C9, 1:200, Cell Signaling #3014), mouse anti-insulin (L6B10 ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were then incubated overnight on a plate rocker at 4° C in a 1:200 dilution of anti-phospho-Erk1/2 (Thr202/Tyr204) rabbit monoclonal antibody (Cell Signaling 4370) in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... GRP78) or anti-rabbit-HRP (TMPRSS2) conjugated secondary antibodies at 1:3000 for 2hrs at room temperature (Cell Signaling, Danvers, MA, USA). Visualization of TMPRSS2 ...
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bioRxiv - Systems Biology 2021Quote: ... one million cells from each library were stained with a 1:25 dilution of anti-HA AlexaFluor647 conjugated antibody (Cell Signaling Technology) in Buffer A (20 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... 20 µg of total brain protein were loaded and anti-GFAP antibody (D1F4Q) XP Rabbit mAb (1:3000; Cell Signaling Technology, 12389), anti-Iba1 antibody (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Neuroscience 2019Quote: ... The vibraslices were then incubated for overnight at RT with rabbit anti-HA antibody (1:1000; Cell Signaling Technology Cat# 3724, RRID:AB_1549585), followed by 1 hr with the Cy5-conjugated goat anti-rabbit IgG ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then stained overnight at 4°C with AlexaFluor 647-conjugated anti-β-catenin antibody L54E2 at 1:300 dilution supplemented with 3% BSA (2677S, Cell Signaling). Between and after each of these steps ...
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bioRxiv - Genetics 2019Quote: ... Membranes were then washed three times with TBS-T and incubated for 1 h at room temperature with anti-rabbit horseradish-peroxidase-conjugated secondary antibody (Cell Signaling, 7074) diluted 1:20000 ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were then resolved on 10% sodium dodecyl sulfate polyacrylamide gels and blots were probed using the following antibodies: rabbit anti-FLAG (Cell Signaling Technology), rabbit anti-V5 (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... The expression of PSMA protein in each cell line was investigated by western blot using anti-PSMA rabbit antibody (Cell Signaling Technology) [11].
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies (0.25% Triton-X, 2.5% normal goat serum, PBS; 24h) against Fos (1:800 rabbit anti-cFos, Cell Signaling #2250; RRID: AB_2247211) or tyrosine hydroxylase (1:400 mouse anti-TH ...
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bioRxiv - Plant Biology 2020Quote: ... or phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (anti-phospho-TEY, #9101, 1:200, Cell Signaling Technology, Danvers, MA, USA). Membranes were washed 3 times for 5 min with TBST and incubated for 1 h with the appropriate secondary antibody – goat anti-rabbit (1:20,000 ...
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bioRxiv - Systems Biology 2021Quote: ... we washed the cells 5 X 5 min with 5% BSA-PBS and then incubated the cells at room temperature for 1 hour in 5% BSA-PBS containing 1:500 goat anti-rabbit secondary antibody conjugated to Alexa Fluor 594 (Cell Signaling, 8889). After the secondary incubation ...
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bioRxiv - Neuroscience 2021Quote: ... Immunofluorescence staining was performed by a standard protocol using an anti-HA rabbit primary antibody (C29F4, Cell Signaling Technology, diluted 1:1600) and a secondary antibody goat anti-rabbit Alexa Fluor 633 (Invitrogen ...
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bioRxiv - Pathology 2021Quote: ... The separated proteins were transferred onto a nitrocellulose membrane for detection with polyclonal rabbit anti-Phospho-Myosin Light Chain 2 (Ser19) antibody (Cell Signaling Technologies) or monoclonal rabbit anti-Myosin Light Chain 2 (D18E2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The neutrophils were then incubated overnight at 4°C with primary antibodies: anti-histone H3 (1:50 dilution; Cell Signaling Technology, 3680) and either anti-NE (1:100 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were lysed in ODG buffer and the lysates were incubated with an anti-Myc-tag antibody (2276, Cell Signaling Technology). Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... The purity of recombinant Bl-Eng2 was assessed by SDS-PAGE and western blot using an anti-His antibody (Cell signaling Technology).
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated at 4°C overnight with the following primary antibodies: rabbit anti-ubiquitin (1:1000; Cell Signaling Technology no. 3933), rabbit anti-phospho-eIF2α (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C with the following primary antibodies diluted 1:1000 in 5% BSA/TBST: anti-pro-IL-1β (#12703, Cell Signaling Technology) and anti-NLRP3 (AG-20B-0014-C100 ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5% Tween-20) and incubated 1 hour at room temperature with 1/2500 of primary antibody anti-pERK (Cat.#4377; Cell Signaling Technology) diluted saline buffer ...
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bioRxiv - Immunology 2020Quote: ... To detect phosphorylated ERM proteins a rabbit anti-phospho–ezrin (Thr567)/radixin (Thr564)/moesin (Thr558) (pERM) antibody was used (Cell Signaling Technology). Isotype control staining was performed using rabbit IgG isotype mAb Alexa Fluor 647 (DA1E ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were labelled overnight with primary antibodies diluted in PBS 10% (w/v) BSA (for anti-RelA: rabbit monoclonal, Cell Signaling Technology #8242 ...
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bioRxiv - Cell Biology 2020Quote: ... Depletion of ATGL16L1 and LC3B was verified on ALI 7 days by immunoblot analysis using antibodies to rabbit anti-ATG16L1 (1:1000 vol;vol ATG16L1 1:1000 vol;vol (Cell Signaling D6D5) or rabbit anti-LC3B (1:500 vol;vol Sigma-Aldrich L7543) ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked for 1 hour at room temperature with blocking buffer (Licor) and incubated overnight at 4°C with anti-K48-linked poly-ubiquitin antibody (Cell Signaling Technologies) and anti-β-actin antibody (Santa Cruz Biotechnologies) ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in antibody solution containing 2% normal donkey serum and 0.3% Triton X-100 in PBS with primary rabbit anti-activated caspase 3 antibody (Cell Signaling, Cat# 9661). Sections were washed five times for five minutes in PBS ...
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bioRxiv - Cancer Biology 2022Quote: The cells surface expression of MDR1 in parental and resistant Y79 cells was detected using an anti-MDR1 antibody (1:500, cat#13342, Cell Signaling Technology). Parental and resistant Y79 cells post-drug exposure was incubated in 200µl of PBS containing 1% FBS and 2ug of MDR1 antibody at 4°C for 1hour in an intermittent shaker ...
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bioRxiv - Biochemistry 2022Quote: ... PDGFRβ was immunoprecipitated from the supernatant with 25 μL of monoclonal anti-HA antibody conjugated to Sepharose beads (Cell Signaling, no. C29F4). KIT and PDGFRβ immunocomplexes were washed three times with washing buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The total p53 and its phosphorylation at Thr55 were detected by anti-p53 antibody (Cell signaling Technology, 1C12, Cat#: 2524, 1:1000) and anti-p53 pThr55 antibody (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... the membrane was incubated overnight at 4°C or with primary antibody solution (1:1000 dilution for anti-Alix [E4T7U, Cell Signaling Technology] ...
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bioRxiv - Biochemistry 2022Quote: ... Unroofed RBCs were blocked for 30 mins at room temperature in blocking buffer and then incubated with 1:200 rabbit anti-HA primary antibody (3724; Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized with 0.3% Triton X-100 in PBS (PBST) prior to blocking and staining with rabbit anti-acetylated alpha tubulin antibody (Cell Signaling #5335). Confocal 3D images of whole islets were used for cilia frequency and length quantitation ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were blocked with 1% BSA plus 10% goat serum for 1 hr and stained with the anti-phospho-paxillin (Tyr118) antibody (Cell Signaling Technology) at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... and a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (1:1000 in blocking buffer for 1 hour, Cell Signaling Technology 7074S) with the peroxidase substrate KPL TrueBlue (30 minutes ...
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bioRxiv - Synthetic Biology 2022Quote: ... monoclonal population were expanded and screened for the activation of the GFP ligand after stimulation with anti-myc antibodies (Cell Signaling Technology) bound to an A/G plate (Thermo Scientific).
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bioRxiv - Neuroscience 2022Quote: ... for 1h at room temperature and incubated over night at 4°C with the following antibodies diluted in blocking solution: anti-RSK2 (1/1000, Cell Signaling Technology); anti-RPS6 (1/1000 ...
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bioRxiv - Microbiology 2022Quote: ... membranes were washed with TBST and incubated for 1 hour at room temperature with anti-β-Actin antibody (Cell Signaling Technology #3700) diluted 1:1,000 in PBS antibody buffer ...
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bioRxiv - Microbiology 2022Quote: ... Our experimental sample used TgBDP13xHA parasites and 1ul of a 1:50 dilution of the rabbit anti-HA primary antibody (Cell Signaling 3724T). The negative control sample used ΔKu80 parasites (the parental line ...
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bioRxiv - Physiology 2022Quote: ... The membranes were subsequently washed 4 times in TBS-T for 10 min before incubating in the appropriate secondary antibody anti-rabbit HRP (1:5000; 1677074P2, Cell Signaling Technology) or anti-mouse HRP (1:5000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Dry peptides were then resuspended in an IAP buffer (Cell Signalling #59322) and incubated with anti-diGly antibody-conjugated slurry (Cell Signaling #59322) at 4°C overnight ...
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bioRxiv - Physiology 2024Quote: ... The membranes were blocked with 10% non-fat dry milk in Tris-buffered saline with Tween-20 for 1 h at room temperature and then incubated with primary antibodies overnight at 4℃ (anti-phospho-PKA, 1:1000, #5661, Cell Signaling Technology, USA ...
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bioRxiv - Molecular Biology 2024Quote: ... To normalize the phospho (Thr389)-p70 S6 Kinase signals membranes were stripped and reprobed with a phosphorylation-independent anti-p70 S6 Kinase antibody (clone 49D7, Cell Signaling Technology). The expression of the “classical” housekeeping genes β-actin and GAPDH is regulated during myogenesis (Hildyard & Wells ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were diluted in PBS-T containing 5% BSA and used 1:1000 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058), 1:1000 for anti-phospho-Akt(Thr308 ...