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Citations for Cell Signaling Technology :
451 - 500 of 506 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos/explants were then blocked in PBS+ 10% FBS and 1% Triton X-100 and incubated overnight at 4°C with an anti-pSmad2/3 antibody (Cell Signaling #8828) at 1:1000 in block ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then washed with CyTOF permeabilization buffer and the barcoded samples were combined before being incubated with antibodies in a solution containing 10% NGS (Cell Signaling #5425). Cells were then washed with CyTOF staining buffer and fixed with 10% formalin (Merck #F5554 ...
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bioRxiv - Immunology 2021Quote: ... For phosphoflow experiments cells were immediately fixed by FoxP3/Transcription Factor Staining Buffer Set (eBioscience) for 10 minutes at 37°C after stimulation and labeled by pErk-AF488 (Cell Signaling, #13214) and pS6-APC (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... the slides were rinsed with PBS and then incubated with ready-to-use IHC detection reagent (Cell signaling, Danvers, MA; 10 µl) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed for 10 minutes thrice with 1×TBST before incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Cancer Biology 2020Quote: ... and the whole fibrin gel channel was collected and immersed for 10 minutes on ice in 25 µL of cell lysis buffer (fresh stock of 10 mL prepared each time from 1X buffer (9803S, Cell Signaling Technologies) with the addition of 1 µL of Benzonase Nuclease (E8263 ...
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bioRxiv - Cancer Biology 2020Quote: ... Ten to 50 μg of proteins were separated by 10 % SDS-PAGE and visualized with primary Slug or E-cadherin antibody (C19G7, Cell Signaling Technology, #9585 ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen brain lysates or hippocampi isolates were then homogenized with a T 10 basic ULTRA-TURRAX (IKa) in ice-cold buffer lysis (Cell Signaling 9803) and protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... the slides were rinsed with PBS and then incubated with ready-to-use IHC detection reagent (Cell signaling, Danvers, MA; 10 μl) at room temperature for one hour ...
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bioRxiv - Microbiology 2019Quote: ... Beads were boiled for 10 minutes at 95 °C and supernatants were subjected to Western blotting for P-Tyr (Cell Signaling 8954).
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were separated by electrophoresis on 10% or 12% SDS-polyacrylamide gels and transferred to PVDF membranes that were probed with anti-pSmad1/5/8 (Cell Signaling 9511S), anti-BMP4 (Santa Cruz sc12721) ...
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bioRxiv - Genomics 2020Quote: ... The samples were blocked in 10% goat serum and incubated overnight at 4°C with the mouse Cas9 antibody (7A9-3A3 Cell Signaling Technologies) 1:800 dilution in 1% BSA (Supp ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were then resolved on 10% sodium dodecyl sulfate polyacrylamide gels and blots were probed using the following antibodies: rabbit anti-FLAG (Cell Signaling Technology), rabbit anti-V5 (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were permeabilized in 0.2% Triton X-100 for 10 min at room temperature following the incubation with anti-FLAG 1:1000 (Cell Signaling Technology, 14793s) and anti-Myc 1:1000 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were permeabilized in 0.2% Triton X-100 for 10 mins at room temperature then incubated with anti-FLAG 1:1000 (Cell Signaling Technology, 14793s) and anti-Myc 1:1000 (Cell Signaling Technology ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were labelled overnight with primary antibodies diluted in PBS 10% (w/v) BSA (for anti-RelA: rabbit monoclonal, Cell Signaling Technology #8242 ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were blocked with 1% BSA plus 10% goat serum for 1 hr and stained with the anti-phospho-paxillin (Tyr118) antibody (Cell Signaling Technology) at 4°C overnight ...
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bioRxiv - Physiology 2022Quote: ... The membranes were subsequently washed 4 times in TBS-T for 10 min before incubating in the appropriate secondary antibody anti-rabbit HRP (1:5000; 1677074P2, Cell Signaling Technology) or anti-mouse HRP (1:5000 ...
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bioRxiv - Developmental Biology 2023Quote: ... in PBS and incubated overnight at 4 °C with primary antibody or isotype control antibody diluted in PBS containing 10% donkey serum (Jackson) or goat serum (Cell Signaling Technology). Secondary antibodies (1:1000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µM of the prolyl hydroxylase inhibitor DMOG and 10 µM of the protease/phosphatase inhibitor MG-132 (Cell signaling; product 5872S) were used to prevent degradation of HIF ...
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bioRxiv - Cell Biology 2023Quote: ... washed with TBST (3 ×10 min), incubated (1 hr, 20°C) in TBST containing HRP-conjugated secondary antibody (1:3000 anti-mouse, Cell Signaling Technology Cat# 7076S ...
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bioRxiv - Bioengineering 2023Quote: ... 45 µL of cells suspension were incubated for 90 min at RT in a total volume of 55 µL with 10 nM BioGCSFR and 1:200 Myc-Tag (9B11) Mouse mAb (2276, Cell Signaling Technology). After one wash with 500 µL PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and samples were prepared by boiling at 99 °C for 10 minutes with Blue Loading Buffer (7722S; Cell Signaling Technology, Danvers, MA). Then ...
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bioRxiv - Genomics 2024Quote: ... Cell lysate was centrifuged at 21,000 x g for 10 minutes and supernatant was mixed with Blue Loading Buffer (Cell Signaling Technology #7722) per the manufacturer’s recommended ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... Final cell pellets were lysed on ice for 10 min in 1X RIPA buffer (cat. no. 9806S, Cell Signaling Technology, Danvers, MA, USA) with added protease inhibitors (cat ...
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bioRxiv - Bioengineering 2021Quote: ... washed three times for 10 min in PBS and incubated for 1 hour in blocking solution (5% normal goat serum (Cell Signaling Technology #5425), 0,3% Triton X-100 in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed with 1× TBST (3 × 10 min) and incubated with anti-mouse IgG horseradish-peroxidase secondary antibody conjugate (1:10000 dilution; Cell Signaling Technology, 7076S) in 1× TBST for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed with Bond Wash solution 2x 10s and blocked with a phosphate-buffered saline (PBS) solution containing 5% normal goat serum (5425S, Cell Signaling, Danvers, MA) and 0.5% TritonX-100 (T8787 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated and on 10% SDS-PAGE gel and blotted onto PVDF membrane and probed with polyclonal phospho-Ser/Thr antibodies (Cell Signaling Technology, USA). Signals were detected using anti-rabbit IgG conjugated with alkaline phosphatase and the color reaction substrates NBT-BCIP (Roche Biochemicals ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was washed 1× TBST three times for 10 min each followed by incubation with anti-rabbit IgG horseradish-peroxidase secondary antibody conjugate (1:3000 dilution; Cell Signaling Technology, 7074S) in 1× TBS for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bound beads were boiled with 1X SDS loading buffer and resolved in 10% SDS-PAGE and analyzed by with 1:1000 dilution of rabbit anti-PAX9 polyclonal antibody (Cell Signaling Technology, USA) or 1:1000 dilution of mouse anti-FLAGM2 monoclonal antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: Co-immunoprecipitation of endogenous PARP1 and SET8 were performed with 200 µg of total extract from crosslinked (1% formaldehyde for 10 min) HCT116 or HeLa cells using anti-PARP1 antibody (Cell Signaling Technology # 9532), anti-SET8 antibody (Santa Cruz Biotechnology # sc-515433 ...
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bioRxiv - Cancer Biology 2022Quote: ... Histone modification ChIPs were performed by using 10 µg of chromatin each incubated with the recommended amount of H3K27Ac (Cell Signaling #8173, 5ul), H3K4me1 (Cell Signaling #5326 ...
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bioRxiv - Neuroscience 2023Quote: ... with 10% horse serum, 1% penicillin-streptomycin (gibco, Cat# 15140-122) and 0.1 ug/mL nerve growth factor (NGF, Cell signaling Technologies, Cat# 84087S). An additional 2-3 mL of culture media was added ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were then suspended in 50 µl Na-Phosphate buffer and incubated at room temperature for 10 minutes with desired antibody [20 µl of CTCF antibody (Cell Signaling Technology #2899) or 11 µg of anti-rabbit IgG (Thermo)] ...
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bioRxiv - Genomics 2023Quote: ... Cells were proceeded for IF as described above using anti-CD56 mouse antibodies (1:10, Coulter #PN4235479-G) and anti-TCF7L2 rabbit antibodies (1:100, Cell Signaling Technology #C48H11), revealed by Cy3-coupled anti-mouse IgGs and Cy5-coupled anti-rabbit IgGs.
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bioRxiv - Molecular Biology 2023Quote: Cells were washed with D-PBS (-) (Wako, 045-29795) and lysed using 140 µL of 10× Cell Lysis buffer (Cell Signaling Technology, 9803S) with PMSF (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was then washed in TBS-Tween three times for 10 min at RT and incubated with the secondary antibody anti-mouse-HRP (1:5000) (Cell Signaling, Danvers, MA) for one hour at RT ...
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bioRxiv - Microbiology 2024Quote: ... buffer (1% FBS in 1x PBS) on ice for 10 minutes and then stained for viability (Ghost DyeTM Red 780; Cell Signaling, Cat# 18452S) on ice for 20 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... then transferring 0.5 mL volume to corresponding cell chambers (see Supplementary Table S1): 10 μM of etoposide (Cell Signaling Technology® Cat. No. 2200S); 0.5 μM of nocodazole (Sigma-Aldrich Cat ...
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bioRxiv - Microbiology 2023Quote: ... secondary antibody staining was performed for 10 minutes at 4°C with Anti-Rabbit IgG conjugated to Alexa Fluor® 488 fluorescent dye (Cell Signaling Technology, 4412S). Finally ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were incubated for 1 hr in blocking buffer (PBS-Triton, FBS 10%) then overnight at 4°C with anti-Yap antibody (Cell Signaling, #4912, 1:200) in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with primary antibody in 10 % Donkey Serum in PBS overnight at 4 °C: Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb (Cell Signaling Technology, Cat# 5364; RRID:AB_10694233). After HSCs were washed three times with PBS + 0.1 % Tween-20 ...
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bioRxiv - Bioengineering 2021Quote: ... The membrane was washed three times with X1 TBST for 10 min each and incubated with HRP□conjugated rabbit or mouse secondary antibodies (#7074 and #7076, Cell Signaling Technology Inc., Danvers, MA) at 1:5000 dilution in X1 TBST with 5% nonfat dry milk for 1 h at RT ...
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bioRxiv - Biophysics 2020Quote: ... M-phase was discriminated with the phospho-Histone H3 (Ser-10) primary antibody (1:25 in PBS with 1% of NGS, Cell Signaling Technology, USA [RRID:AB 331748]) and the secondary anti-mouse IgG antibody (1:500 ...
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bioRxiv - Systems Biology 2022Quote: ... an Odyssey blocking buffer solution containing 10% OptiPrep™ and 1:2000 Alexa 488-conjugated anti-phospho-histone H3 (S10) antibody (pH3) (clone D2C8, Cell Signaling Technology, Danvers, MA) to label M-phase cells and 2 µg/ml Hoechst 33342 to stain all nuclei ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting supernatants (10 μL) were analyzed by Western Blot with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling, 1679101S; dilution 1:1,000) primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were washed with TBST for 10 min three times before incubation with secondary antibodies [Anti-rabbit IgG-HRP (Cell Signaling Technology 7074S, 1:5000), Anti-mouse IgG-HRP (Cell Signaling Technology 7076S ...