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Citations for Cell Signaling Technology :
451 - 500 of 10000+ citations for 5 5 7 7 Tetramethyl 1 5 6 7 tetrahydrocyclopenta f indazol 4 amine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... which was then blocked with 5% milk and blotted using anti-mTOR antibody (Cell Signaling Technologies) as primary antibody ...
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bioRxiv - Bioengineering 2021Quote: ... washed three times for 10 min in PBS and incubated for 1 hour in blocking solution (5% normal goat serum (Cell Signaling Technology #5425), 0,3% Triton X-100 in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Histological sections (5 mm) were stained for immunohistochemistry using a standard protocol with antibodies for LC3A/B (1:200; #12741S; Cell Signaling Technology, USA).
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% skimmed milk and incubated with primary antibodies (rabbit anti-PPAR-γ antibody, Cell Signaling Technology, 1:1000) and HRP-conjugated goat anti-rabbit IgG ...
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bioRxiv - Synthetic Biology 2023Quote: ... Primary staining was performed on separate blots with the following antibody dilutions in 5% BSA/TBST: rabbit anti-EZH2 1:1000 (Cell Signaling Technology, 5246S), rabbit anti-CBX2 1:500 (Bethyl Laboratories ...
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bioRxiv - Physiology 2023Quote: ... was heated at 90°C for 5 minutes and loaded into acrylamide gels before incubated with primary antibodies against catalase (CAT, #14097, Cell Signaling, 1:5000), 3-nitrotyrosine (3-NT ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in 5% skim milk in TBST: horse anti-mouse IgG (1:20,000, Cell Signaling Technology, cat# 7076), donkey anti-sheep IgG (1:20,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... and blocked in room temperature with 5% BSA (HiMedia, India) solution for an hour and incubated with respective primary antibodies [FOXM1 (1:2000) (Cell Signaling Technologies, USA); active + pro Caspase-3 (1:3000 ...
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bioRxiv - Biochemistry 2023Quote: The antibodies used in this study for immunoblotting (Figure 5) were (1) GST-Tag monoclonal antibody 26H1 (Cell Signaling Technologies catalog number 2624S); (2 ...
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bioRxiv - Cell Biology 2024Quote: ... and blocked with 1x TBST + 5% normal goat serum (NGS) cocktail for 60 min at RT before applying the NFκB primary antibody (Cell Signaling, 6956, 1:1000) in 1x TBST cocktail containing 1% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: ... The membranes were blocked with PBS-Milk 5% for 30 min at RT before staining with primary antibody against HA-tag (C29F4, Cell signaling, 1:500) and Actin (ab49900 ...
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bioRxiv - Neuroscience 2024Quote: ... Blocking was performed with 5% non-fat milk dissolved in TBST for 1 hour followed by incubation with primary antibodies PRKN (Cell Signaling Technology, 2132) at 1:2000 dilution in 5% BSA in TBST at 4 °C overnight and VCL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The sections were washed twice with PBS and incubated in 2% BSA plus TBS with primary antibody [1:80 dilution rabbit anti-5-mC antibody (Cell Signaling Technology, #D3S2Z) at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... and proteins of interest were analyzed using primary antibodies (1:1000 in 5% BSA in TBS-T) as follows: anti-LKB1 (Cell Signaling Technology; 3050S), anti-phospho-LKB1 (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were blocked with 5% skim milk in TBST for 1 h at room temperature and incubated with anti-human p53 antibody (Cell Signaling Technologies, #9282, rabbit polyclonal, 1:1000) in TBST containing 5% skim milk (4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 0.2% TritonX-100 and 5% Normal Goat Serum) of rabbit anti-c-Fos (Cell Signaling, #2250,1:700) for two days at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... blocked in 5% milk and subjected to Western blot analysis using antibodies for Notch2 (Cell Signaling, D76A6) and GAPDH (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with PBS then permeabilized and blocked with 5% normal goat serum (Cell Signaling Technology) and 1% BSA in PBS containing 0.3% Triton X-100 (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... Spheroids were then washed with DPBS and blocked with 5% Normal Goat Serum (NGS) (Cell Signaling Technology) for 1 h ...
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bioRxiv - Immunology 2020Quote: Cell lysates from 5-10 × 106 cells were generated using CHAPS Cell Extract Buffer (Cell Signaling Technology) supplemented with 1X Halt protease inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were diluted in 5% BSA/TBS as follows: rabbit anti-Cx43 (Cat. No. 3512; Cell Signaling) at 1:2000 and mouse anti-Vinculin (Cat ...
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bioRxiv - Immunology 2023Quote: ... blocked with 5% BSA and incubated with monoclonal rabbit anti-HIF-1α (Cell Signaling Technology, clone D1S7W). Detection was carried out with a goat anti-rabbit horse radish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 7.4) supplemented with 5 % BSA and HRP-conjugated rabbit anti-mouse antibody (Cell Signaling Technologies, USA) diluted 1:2000 as a secondary antibody.
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked in 5% milk following an incubation with anti-HA antibody (Cell Signaling Technology, #3724) to detect EPHB2 ubiquitination levels ...
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bioRxiv - Genomics 2024Quote: ... solubilized chromatin was recovered and incubated with 5 μg of Pol II antibody (#14958, Cell Signaling Technology) bound to Dynabeads M-280 sheep anti-Rabbit IgG (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2021Quote: ... which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST) and incubated with rabbit anti-pAkt antibody (Cell Signaling Technology, 4060S, 1:1,000 dilution) or rabbit anti-AKT antibody (Cell Signaling Technology 9272S ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-Actin (Cell Signaling Technology, #3700, 1:4000, Danvers, MA, USA), c-Myc (Cell Signaling Technology ...
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bioRxiv - Biophysics 2020Quote: ... Cells were then resuspended in 100 μl PBS-FC containing 5% FBS with 1:100 diluted PE-conjugated anti-myc (9B11) mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA) and incubated for 30 min in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 5% BSA in TBS-T before incubation with primary antibodies: phospho-AMPKα Thr172 (1:1000, Cell signaling, MA, USA #2535), β-actin (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was blocked for 2-hours in 1% NGS and 0.3% Triton X-100 followed by 2-3 days of 4C° incubation in 1xPBS (5% NGS, 0.3% Triton X-100) with primary antibody(ies): pS6 S235-236 (Cell Signaling, Catalog: 4858, 1:500), pS6 S244-247(ThermoFisher ...
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FGF signalling is involved in cumulus migration in the common house spider Parasteatoda tepidariorumbioRxiv - Developmental Biology 2021Quote: ... Fluorescent pMad antibody staining was performed using the Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Cell Signaling Technology, Inc.; antibody concentration: 1:1000) as primary and an Alexa488 anti rabbit AB (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... the membranes were washed 3 times in TBS-T for 5 minutes and incubated for 1 hour in HRP-conjugated anti-rabbit antibodies (Cell Signaling Technology, London, UK) at dilution of 1:5000-1:10,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated with the following primary antibodies for 2 hours at room temperature in 5% BSA: 1:1000 anti-SMARCC1 (Cell Signaling Technologies cat. #11956T), 1:1000 anti-SMARCA4 (Cell Signaling Technologies cat ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: 5 μM thick paraffin-embedded liver sections were used for hematoxylin and eosin (H&E) staining and Ki67 (Cell signaling technology 12202S, 1:400) immunohistochemical staining ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked in Tris-buffered saline/0.1% Tween (TBST)/5% milk and probed with antibodies against E-Cadherin (Cell Signaling Technology, 3195S, 1:1000 dilution), Vimentin (Cell Signaling Technology ...
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bioRxiv - Physiology 2024Quote: ... membranes were washed 3 times for 5 minutes and incubated for 1 hour in HRP-conjugated anti-rabbit antibody (Cell Signaling Technology, London, UK) at a dilution of 1:20,000 in non-fat dry milk ...
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bioRxiv - Biochemistry 2024Quote: ... Anti- mouse, Cat. No. 7076; 1: 10 000 in 5% skim milk/TBS-T, Anti-rabbit, Cat. No. 7074S, Cell Signaling Technology, Massachusetts, US) and Pierce ECL Western Blotting Substrate (Cat ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then washed three times for 5 minutes each with 1X TBST and incubated with a secondary anti-rabbit horseradish peroxidase-conjugated antibody (Cell Signaling, Cat # 7074S, 1:2000) in 5% BSA for two hours at room temperature with gentle rocking ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was washed again 4 times in TBST for 5 m each and blocked again before incubating with primary antibodies: total Akt (1:1000, Cell Signaling Technology, Cat. #9272S, RRID:AB_329827) & total S6RP (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody dilutions were prepared in 5% BSA-TBST and incubated overnight with gentle agitation at the following dilutions phospho-AKT T308 1:2000 (Cell Signaling Technology Cat# 13038, RRID:AB_2629447), AKT 1:1000 (Cell Signaling Technology Cat# 9272 ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were washed three times in TBST for 5 minutes and incubated for 1 hour with horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology Cat# 7074, RRID:AB_2099233) or anti-mouse IgG (Cell Signaling Technology Cat# 7076 ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated for 2 h in a solution of TBST (0.05%) and LI-COR blocking buffer (5%) containing the following antibodies: anti-ERK1/2 (Cell Signaling Technologies; dilution factor: 1/2000) and streptavidin IRdye680 (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed three times with TBST for 5 minutes and immunoblotted with the appropriate HRP-conjugated secondary antibodies: anti-rabbit (Cell Signaling, Cat#7074, 1:2000) or anti-mouse (Cell Signaling ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was washed with TBST and incubated in TBST + 5% (w/v) nonfat milk and anti-rabbit IgG HRP-linked antibody (Cell Signaling catalog #7074, 1:1000) for 1 hour then washed in TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... we developed our RiboAb cocktail (20 µL used at a dilution of 1:1000) containing 5 µL rpl5 (Cell Signaling, Danvers, MA, USA, cat #: 14568S), 5 µL rpl11 (Cell Signaling ...
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bioRxiv - Biochemistry 2020Quote: ... cells were permeabilized and blocked for 1h with DPBS containing 5% goat serum (Cell Signaling Technology, Cat# 5425S) and 0.1% Triton X-100 and then incubated with anti-HA antibodies at 1:500 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-actin (Cell Signaling Technology, #3700 ...
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bioRxiv - Immunology 2022Quote: ... Alexa Fluor 647 (Ax647)- or cyanin 5 (Cy5)-labeled goat- or anti-rabbit antibodies purchased from Cell Signaling.
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked with a blocking buffer of 5% non-fat milk powder (Cell Signaling Technology, Danvers, MA) in phosphate buffered saline with 0.05% tween-20 (PBS-T ...
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bioRxiv - Molecular Biology 2020Quote: ... The chromatin was then incubated with 5 mg of STAT1 antibody (Cell Signaling Technologies, Danvers, MA; CST-9172) for every ½ plate of cells at 4 °C overnight.