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Citations for Cell Signaling Technology :
451 - 500 of 10000+ citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... blots were incubated overnight at 4°C in the following primary antibodies: β-III-Tubulin (TUJ1, 1:1000; Cell Signaling Technology, 5568), SUFU (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with primary anti-RAB5 antibody at 4 °C overnight diluted in 8% BSA in PBST (1:500 dilution, Cat. #3547, lot 7, Cell Signaling Technology). Secondary anti-rabbit Alexa Fluor 568 antibody (1:1000 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... the membrane was incubated overnight at 4°C or with primary antibody solution (1:1000 dilution for anti-Alix [E4T7U, Cell Signaling Technology] ...
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bioRxiv - Neuroscience 2022Quote: ... for 1h at room temperature and incubated over night at 4°C with the following antibodies diluted in blocking solution: anti-RSK2 (1/1000, Cell Signaling Technology); anti-RPS6 (1/1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1 ...
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bioRxiv - Biophysics 2023Quote: ... Cells were incubated overnight at 4°C with the primary antibody against VE-Cad (rabbit mAb, D87F2, Cell Signaling Technology, 1:200) diluted in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... and stained 1h/RT or overnight/4°C with following primary antibodies in 0,2% Triton-X100 in PBS: PAX6 (rabbit, CS60433, Cell Signaling, 1:300), ZO-1-1A12 (mouse ...
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bioRxiv - Pathology 2024Quote: ... and incubated overnight at 4°C with the following primary antibodies: rabbit anti-STING (1:1000; #13647, Cell Signaling; Danvers, MA, USA), rabbit anti-TBK1 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... after which they were incubated overnight at 4°C with rabbit anti-c-fos (1:500, Cell Signaling Technology, catalog no. 2250S). Sections were then washed with PBS ...
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bioRxiv - Bioengineering 2024Quote: Membranes were subsequently incubated overnight at 4°C with the following primary antibodies: rabbit anti-GAPDH (1:1,000, Cell Signaling Technology #2118) or rabbit anti-HTT ...
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bioRxiv - Immunology 2024Quote: ... The cells were stained overnight at 4 °C with the following primary antibodies: anti-NFAT1 (1:200, cat. 4389, Cell Signaling Technology), anti-NFAT2 (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in 4°C overnight in primary antibody for c-Fos (1:750, Rabbit mAb Cell Signaling Technology, cat #2250). The following day ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were incubated overnight at 4 °C with antibodies specific to phospho-histone H3 (Ser10) at a dilution of 1:100 (Cell Signaling Technology). After the primary antibody incubation ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were blocked with 0.2% bovine serum albumin and incubated overnight at 4°C with the anti-EEA (1:200; Cell Signaling Technology, 3288) or anti-LAMP1 (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then incubated at 4 °C overnight with the following primary antibodies at 1:100 dilutions: anti-Ikzf1 (rabbit, D6N9Y, Cell Signaling Technology), anti-CD31 (mouse ...
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bioRxiv - Cancer Biology 2024Quote: ... The blots were then incubated separately with respective primary antibodies for overnight at 4°C [ZEB1 (1:3000) (Cell Signaling Technologies, USA); GPX4 (1:3000 ...
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bioRxiv - Molecular Biology 2024Quote: ... and for Caspase 3/7 1:300 [Cleaved Caspase-3 (Asp175) (5A1E) (Cell Signaling)] ...
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bioRxiv - Neuroscience 2020Quote: ... Blocking buffer was manually removed and replaced with either anti-Asymmetric Di-Methyl Arginine antibody (1:500, Cell Signaling Technology 13522) or anti-Symmetric Di-Methyl Arginine antibody (1:800 Cell Signaling Technology 13222 ...
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bioRxiv - Neuroscience 2024Quote: ... stained for 2-3 days with the total ERK antibody (Cell Signaling, #4696) and phospho-Erk antibody (Cell Signaling #4370) ...
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bioRxiv - Microbiology 2024Quote: ... For visualization of SARS-CoV-2 infected cells via detection of N protein in Calu-3 cells we used anti SARS-CoV-1/2 Nucleocapsid Mouse anti-N clone (Cell Signaling, 1C7C7) 1:1000 50uL/well.
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bioRxiv - Neuroscience 2020Quote: ... The membranes were probed overnight at 4°C using primary antibodies against p-eIF2α S51 (rabbit, Cell Signaling cat no: 9721, 1:500), anti-t-eIF2α (rabbit ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were then fixed with 4% paraformaldehyde and processed for immunostaining with EGFR (D38B1) XP rabbit mAb (4267, Cell Signaling Technology, 1:100), anti-rabbit secondary antibody Alexa Fluor 555 Conjugate (4413 ...
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bioRxiv - Cancer Biology 2022Quote: Whole cell protein was extracted from cells treated for 24 h with 1 mM aspirin and 4 μM regorafenib using radio-immunoprecipitation assay buffer (Cell Signaling Cat# 9806) supplemented with 1 mM phenylmethanesulfonyl fluoride (Cell Signaling Cat# 8553) ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked followed by overnight incubation at 4°C with primary antibodies: C-terminal Parkin antibody (PRK8; Cell Signaling, 1:40,000 dilution) and N-terminal Parkin antibody (https://mrcppureagents.dundee.ac.uk/reagents-view-antibodies/589302 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated for 48h at 4°C with the following primary antibodies: rabbit anti-phospho-S6 (1:1000, Cell Signaling, Cat# 5364), mouse anti-NeuN (1:400 ...
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bioRxiv - Cancer Biology 2020Quote: ... for 2 hours and blotted at 4°C overnight with primary antibodies as follows: rabbit anti-phospho-GSK-3β (Ser9) (5B3) (1/500 dilution; Cell Signaling; cat #9323), rabbit anti-GSK-3β (1/1000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were probed overnight at 4°C in Odyssey Blocking Buffer with antibodies recognizing PS6 (Ser240/244; rabbit monoclonal; 1:1000; Cell Signaling, Danvers, MA), total S6 ribosomal protein (mouse monoclonal ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... and blocked with 5% skimmed milk in TBST for 1 h followed by O/N incubation at 4 °C with appropriate primary antibody: pSTAT3 (D3A7; Cell Signaling Technology, 9145S) and STAT3 (124H6 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rabbit anti-Ezh2 (Cell Signaling Technologies; 1:1000), rabbit anti-H3K27me3 (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4°C for 18-22 hr in a cocktail comprising anti-GSK3β mouse monoclonal antibody (1:1500; #9832, Cell Signaling, Rockford, IL), anti-phospho GSK3β (Ser9 ...
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bioRxiv - Neuroscience 2022Quote: ... 200,000 Bead-bound cells were incubated with primary antibody overnight at 4°C (1:100 Acetyl-Histone H3 (Lys27) Rabbit mAb (Cell Signaling Technology #8173), 1:50 Acetyl-Histone H3 (Lys9 ...
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bioRxiv - Cell Biology 2024Quote: ... were then added to the membrane and incubated overnight at 4°C: anti-Calnexin (1:1,000 dilution, clone C5C9, Cell Signaling Technology, Danvers, MA), anti-Mitofilin (1:500 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated overnight at 4°C with the following primary antibodies: anti-AMPKα (Cell Signaling Technology, USA; diluted at 1:1000), anti-p- AMPKα (Thr172 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then immunoblotted over night at 4°C with the indicated primary antibodies diluted in blocking buffer: Phosphorylated Stat1 (Tyr701) (D4A7) (Cell Signaling, 1:1000), STAT1 (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... The incubation was performed using the free-floating method at 4°C (48 h) with rabbit anti-p75NTR(1:500; Cell signaling, MA, USA) with 0.6% Triton X-100 in PBS with 5% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with hematoxylin-eosin staining for 10min or overnight at 4℃ using the following primary antibodies: β-catenin (1:200, #8480, Cell Signaling Technology), Ki67 (1:400 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked in 5% BSA with 1% Tween 20 for 1 hour then probed overnight at 4°C with the following primary antibodies at a 1:1,000 dilution: Phospho-STAT1 (Cell Signaling Technologies, 9167S, monoclonal), SOCS1 (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with anti-HA antibody (Cell Signaling Technology, 1:100). The next day ...
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bioRxiv - Physiology 2024Quote: ... for one hour at room temperature before being incubated overnight at 4°C with the following primary antibodies: anti-phospho-p38 (1:1,000, #9211, Cell Signaling Technology, Billerica, MA); anti-p38 (1:1,000 ...
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bioRxiv - Neuroscience 2024Quote: ... All sections were incubated overnight at 4⁰ C with rabbit anti-c-Fos primary antibody (5348, 1:2000; Cell Signaling Technology, RRID: AB_10557109) diluted in 2% NHS in PBS-Tx.
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bioRxiv - Cancer Biology 2024Quote: ... Then 300 µl of lysate was immunoprecipitated by rotating overnight at 4° C with rabbit anti-HA epitope (Cell Signaling #3724, 1:100) for exogenous cyclin B ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated overnight at 4 °C with rabbit anti-c-fos primary antibody (1:500; Cell Signaling Technology, catalog no. 2250, USA) in a permeable buffer containing 2% donkey serum ...
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bioRxiv - Bioengineering 2024Quote: ... for an hour with rocking at room temperature and were incubated with the following primary antibodies for overnight with rocking at 4 °C: anti-phosphoSRC(Tyr416) (1:1000, Cell Signaling Technology, #2101), anti-p110α (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were incubated with primary antibody in TBST overnight at 4°C: Phospho-Akt (Ser473) antibody (Cell Signaling Technology, #9271, 1:1000), Akt (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... incubated with primary antibodies overnight at 4 (pS6 Ser240/244 Cell Signaling Technologies (CST) #5364 dilution 1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibody incubation occurred overnight at 4°C for DR4 (Cell Signaling Technology, 42533) and DR5 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... it was incubated overnight at 4°C with an IRE1α antibody (Cell Signaling, #3294S) at 1:1000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with overnight in 4°C using vimentin antibody (Cell Signaling Technologies). Cells were rinsed with 0.2% BSA 3 times for 5 minutes and were then incubated with a fluorescent secondary antibody in 1% BSA and 0.025% saponin buffer for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Three replicates of extract received 4 μg of anti-SIRT6 antibody (Cell Signaling #1248) or anti-LaminA (Lamin A/C-Millipore 05-714 ...
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bioRxiv - Genetics 2020Quote: ... Protein lysates were rotated overnight at 4 °C with anti-myc (Cell Signaling; 9B11), anti-HA antibodies (Cell Signaling ...