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Citations for Jena Bioscience :
1 - 50 of 107 citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Nucleotide binding was analyzed using the non-hydrolysable GTP analogue N- methylanthraniloyl guanosine-5’-[(β,γ)-imido]triphosphate (mant-GMPPNP, Jena Bioscience). Varying protein concentrations of non-farnesylated GBP1 ranging from 0.02 µM to 40 µM were prepared in a 96-well plate in 50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Immunology 2024Quote: ... Immunostaining was performed in four steps to avoid unspecific binding of the secondary antibodies: i) incubation overnight at 4LJ with the mouse monoclonal IgG2a J2 antibody anti-dsRNA (Jena Bioscience, cat. RNT-SCI-10010500, dilution 1:200), ii ...
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bioRxiv - Microbiology 2020Quote: ... For phasing the crystals were soaked overnight in 50 mM 5-amino-2,4,6-triiodoisophthalic acid (I3C)/LiOH (Magic Triangle, Jena Biosciences)42 in cryobuffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Binding was measured between EDA-GTP-Cy5 (Jena Bioscience GmbH, Jena, Germany) and His-tagged labeled proteins in Buffer I ...
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bioRxiv - Microbiology 2020Quote: ... ATP binding assays were performed according to the manufacturer’s protocol (Jena Biosciences). Here we used 6AH-ATP-Agarose (N6 - (6-Amino ...
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bioRxiv - Cell Biology 2020Quote: ... The incorporated EdU was visualized by click-it chemistry-mediated binding of Biotin (Azide-PEG3-Biotin Conjugate; Jena Biosciences) using the protocol and reagents from the Invitrogen Click-iT EdU Cell Proliferation Kit for Imaging (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: Nucleotide release and binding measurements were performed using the fluorescent nucleotide analogues MABA-ADP (Jena Bioscience, NU-893-MNT) and MABA-ATP (Jena Bioscience ...
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bioRxiv - Genetics 2021Quote: ... or under amino acid-selective conditions in complete supplemental medium (CSM) (Difco yeast nitrogen extract without amino acids, amino acid powder from Sunrise Science Products, 2% μg/ml nourseothricin (Jena Bioscience, Jena, Germany) and 2 mM methionine was used to select strains containing pBV65 vector derivatives (42) ...
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bioRxiv - Microbiology 2021Quote: To verify the functionality of mutated SoGGDEF proteins they were tested for GTP binding using MANT-GTP (Jena Bioscience, Germany). Proteins were diluted to 1 μM and 100 μl of each sample was added in triplicates into a white round bottom 96-well plate before MANT-GTP with a final concentration of 1 μM was added ...
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bioRxiv - Cell Biology 2022Quote: Nucleotide binding assays were carried out by measuring the incorporation of 2,3-0-N-Methyl-anthraniloyl (Mant)-labelled nucleotides (Jena Bioscience) using an Synergy2 plate reader (BioTek Instruments Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... 3125 µM ascorbic acid and 125 µM Picolyl-Alexa647 (Jena Bioscience) were added to the mix and incubated for two hours at 37°C while smoothly shaking at 400 rpm ...
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bioRxiv - Biophysics 2020Quote: ... of λ-DNA using the following primers: 5’-CGAACTCTTCAAATTCTTCTTCCA-3’ and 5’-GATTGCTCTTCTGTAAGGTTTTG-3’ with a 5:1 ratio of dTTP:biotin-11-dUTP (Jena Bioscience). Similarly ...
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bioRxiv - Molecular Biology 2023Quote: ... Additional 5-Ethynyl-uridine (5-EU) was purchased from Jena Bioscience (CLK-N002) and resuspended to an initial stock concentration of 400 mM in DMSO.
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bioRxiv - Molecular Biology 2019Quote: ... 5 % CO2 for 1 h after a final concentration of 500 µM 5-Ethynyluridine (5EU, Jena Bioscience) was added ...
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bioRxiv - Microbiology 2019Quote: ... 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM AF647-Picolyl-azide (Jena Biosciences) and 10 mM sodium ascorbate (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... 2’-deoxythymidine-5’-[α-thio]-triphosphate (dTTPαS) and 2’-deoxyguanosine-5’-[α-thio]-triphosphate (dGTPαS) were obtained from Jena Biosciences. N-methylanthraniloyl guanosine-5’-triphosphate (mant-GTP ...
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bioRxiv - Cell Biology 2019Quote: ... nascent RNA was labelled by incubating the cells with 400 μM 5-ethynyluridine (5-EU; Jena Bioscience; CLK-N002-10,), which was then visualized with a click-iT mix consisting of 50mM Tris buffer pH8 ...
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bioRxiv - Molecular Biology 2022Quote: ... in the presence of 0.4μL 5-(3-Aminoallyl)-uridine-5’-triphosphate labeled with ATTO 680(Aminoallyl-UTP-ATTO-680, NU-821-680, Jena bioscience) in each reaction ...
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bioRxiv - Plant Biology 2019Quote: ... 5-Ethynyl-UTP was purchased from Jena Bioscience. A fraction of the reaction was analyzed using a standard agarose gel ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience) at 2 mM and MgCl2 at 4 mM was incubated for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 5’-guanylyl imidodiphosphate (GDPNP; Jena Bioscience NU-401-50) was added to the wells 16 minutes after the start of the reaction for five minutes at a concentration of 100uM followed by a five-minute pretreatment of either 208uM emetine (Cayman Chemical 21048 ...
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bioRxiv - Cell Biology 2022Quote: ... and 5 mM ATP (Jena Biosciences; NU-1010-SOL)) supplemented with 220 nM Sytox Orange at 100 μl/min ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 μM ppGpp (Guanosine-3’,5’-bisdiphosphate) (Jena Bioscience).
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bioRxiv - Immunology 2023Quote: ... 10 μM EdU (5-ethynyl-2’-deoxyuridine, Jena Bioscience) was added to the cell culture medium for 10 min at the end of 72 h culture on anti-CD3ε- and anti-CD28-coated cell culture plates ...
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bioRxiv - Microbiology 2023Quote: ... 5 µl biotin-11-CTP (1 mM) (Jena Bioscience), 0.8 µl DTT (0.5 M ...
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bioRxiv - Cell Biology 2023Quote: ... 5-fold molar excess of DBCO-AF647 (Jena Bioscience) was added to the azide-coupled Nb followed by incubation for 2 hours at room temperature and subsequently purified by dialysis and hydrophobic interaction chromatography (HIC).
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bioRxiv - Genomics 2023Quote: ... N1-Methylpseudouridine-5’-Triphosphate (m1meΨTP, Jena Bioscience, NU-890S) instead of UTP or N4-Acetyl-cytidine-5’-triphosphate (N4-Acetyl-CTP ...
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bioRxiv - Zoology 2020Quote: ... in which a third of the dTTP had been replaced with Digoxigenin-X-(5-aminoallyl)-2’-deoxyuridine-5’-triphosphate (Jena Bioscience, Jena, Germany) for 40 cycles ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyguanosine-5’-triphosphate (3’-dGTP, Jena Bioscience) and 4 mM MgCl2 ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience), 4 mM MgCl2 and 2 μM G-actin ...
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bioRxiv - Neuroscience 2023Quote: ... 0,2 µM 5/6 TAMRA-PEG4-Alkyne tag (Jena Biosciences) and 0,2 mM freshly prepared CuSO4 ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM of m7GP3G monomethylated cap analogue (Jena Bioscience, NU-852) was soaked into mature NSP14 crystals (grown in 1.26 M sodium phosphate monobasic ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with 5’ethynyluridine (EU) (0.5 mM) (Jena Bioscience) and Click-iT Alexa Fluor 647 labelling kit (Thermofisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatants from the centrifugation of the disrupted cells were incubated with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) agarose (Jena Bioscience) on ice for 30 min ...
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bioRxiv - Immunology 2023Quote: ... anti-dsRNA monoclonal antibody (Jena Bioscience, Germany ...
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bioRxiv - Genomics 2022Quote: ... with 50% supplement of 5-Methyl-CTP and Pseudo-UTP (Jena Biosciences), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... and N1-methyladenoside 5’-phosphate (N1-methyl-AMP) was from Jena Bioscience. MTase-Glo™ Methyltransferase Assay Kit containing SAM and SAH were purchased from Promega ...
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bioRxiv - Cancer Biology 2023Quote: EDA-m7GTP-5-FAM (NU-824-5FM) was purchased from Jena Bioscience and used as received ...
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bioRxiv - Immunology 2023Quote: ... Cells were pulsed with OPP (20μM, Jena Bioscience, cat: NU-931-5) in complete RPMI supplemented with the cytoplasmic translation inhibitor harringtonine (1μM ...
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bioRxiv - Biophysics 2019Quote: ... Cy3 labeled 2’/3’-O-(2-aminoethyl-carbamoyl)-adenosine-5’-triphosphate (Jena Bioscience) was added into the imaging buffer instead of ATP.
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bioRxiv - Biochemistry 2021Quote: ... For labeling pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl)phosphate (Jena bioscience), was ligated to the 3’ end of the 16 nt RNA using T4 RNA ligase (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 30 min incubation with 50μM OPP (Jena Bioscience, NU-913-5). Cultures were then washed with room temperature PBS and fixed with 4 % of PFA ...
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bioRxiv - Cell Biology 2023Quote: ... This media was changed and ATP (5 mM) (Jena Biosciences cat. NU-1010) was added for 24 h prior to collection of conditioned media ...
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bioRxiv - Cell Biology 2023Quote: ... This media was changed and ATP (5 mM) (Jena Biosciences cat. NU-1010) was added for 24 h prior to collection of conditioned media ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM of each triphosphate ribonucleotide (standard nucleotides were purchased from Jena Bioscience GmBH and pseudouridine and N1-methylpseudouridine from BOC sciences) ...
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bioRxiv - Neuroscience 2020Quote: ... AMP-CPP (α,β-methyleneadenosine 5’-triphosphate lithium salt, Jena Bioscience #NU-421-25), AMP-PCP (β,γ-Methyleneadenosine 5′-triphosphate disodium salt ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 μM 5/6- TAMRA-PEG 4 -Alkyne (Jena Bioscience; Jena, Germany; CLK-TA108) and 0.2 mM freshly prepared CuSO4 in 1x PBS pH7.8 ...
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bioRxiv - Plant Biology 2023Quote: ... DNA oligonucleotides used as probes were extended with 5-Azido-PEG4-dCTP (Jena Bioscience) and terminal transferase (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 % of UTP (resp. GTP) was substituted with either 5-ethynyl-UTP (5EU, Jena Bioscience), 5-bromo-UTP (5BrU ...