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Citations for Jena Bioscience :
1 - 50 of 195 citations for Retinol Binding Protein RBP Multi format ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: To verify the functionality of mutated SoGGDEF proteins they were tested for GTP binding using MANT-GTP (Jena Bioscience, Germany). Proteins were diluted to 1 μM and 100 μl of each sample was added in triplicates into a white round bottom 96-well plate before MANT-GTP with a final concentration of 1 μM was added ...
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bioRxiv - Biochemistry 2023Quote: Nucleotide binding was analyzed using the non-hydrolysable GTP analogue N- methylanthraniloyl guanosine-5’-[(β,γ)-imido]triphosphate (mant-GMPPNP, Jena Bioscience). Varying protein concentrations of non-farnesylated GBP1 ranging from 0.02 µM to 40 µM were prepared in a 96-well plate in 50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Plant Biology 2023Quote: ... then 5×106 cells were plated on ESAW agar plates containing 450 μg mL-1 nourseothricin (Jena Bioscience), and incubated in constant light at 18 °C.
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bioRxiv - Cancer Biology 2020Quote: ... Binding was measured between EDA-GTP-Cy5 (Jena Bioscience GmbH, Jena, Germany) and His-tagged labeled proteins in Buffer I ...
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bioRxiv - Microbiology 2020Quote: ... ATP binding assays were performed according to the manufacturer’s protocol (Jena Biosciences). Here we used 6AH-ATP-Agarose (N6 - (6-Amino ...
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bioRxiv - Microbiology 2023Quote: ... 20 μl of the prepared protein were mixed with 5 μl of 500-fold diluted JBS Thermofluor Dye (Jena Bioscience), prepared in the same buffer ...
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bioRxiv - Molecular Biology 2022Quote: C15-az and C17-az modified proteins were pulled down using the Click Chemistry Capture Kit (Jena Bioscience) and alkyne agarose beads (Jena Bioscience) ...
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bioRxiv - Cell Biology 2020Quote: ... The incorporated EdU was visualized by click-it chemistry-mediated binding of Biotin (Azide-PEG3-Biotin Conjugate; Jena Biosciences) using the protocol and reagents from the Invitrogen Click-iT EdU Cell Proliferation Kit for Imaging (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: Nucleotide release and binding measurements were performed using the fluorescent nucleotide analogues MABA-ADP (Jena Bioscience, NU-893-MNT) and MABA-ATP (Jena Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... The assay was performed as per kit instructions using a Tecan Spark 10M multimode plate reader and ATP (nM) was determined using the ATP (Jena Bioscience) standard curve produced.
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bioRxiv - Genetics 2021Quote: ... the PCR labelling Cy-3 and Cy-5 kits (Jena Bioscience, ref# PP-301L-Cy3 and #APP-101-Cy5) according to the manufacturer’s instructions with M13 universal primers ...
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bioRxiv - Cell Biology 2022Quote: Nucleotide binding assays were carried out by measuring the incorporation of 2,3-0-N-Methyl-anthraniloyl (Mant)-labelled nucleotides (Jena Bioscience) using an Synergy2 plate reader (BioTek Instruments Inc. ...
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bioRxiv - Biophysics 2021Quote: ... initial crystallization conditions were screened on sitting-drop Greiner 609120 96-well plates using a Honeybee963 robot (Digilab) and commercial kits from Jena Bioscience (Jena) and Hampton Research (Aliso Viejo ...
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bioRxiv - Biophysics 2020Quote: ... of λ-DNA using the following primers: 5’-CGAACTCTTCAAATTCTTCTTCCA-3’ and 5’-GATTGCTCTTCTGTAAGGTTTTG-3’ with a 5:1 ratio of dTTP:biotin-11-dUTP (Jena Bioscience). Similarly ...
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bioRxiv - Biochemistry 2022Quote: Initial crystallization conditions for P9-1ΔC-arm were screened at room temperature on 96-well sitting-drop Greiner 609120 plates using a Digilab Honeybee963 robot (Marlborough, USA) and commercial kits from Jena Bioscience (Jena, Germany) and Hampton Research (Aliso Viejo ...
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bioRxiv - Molecular Biology 2023Quote: ... Additional 5-Ethynyl-uridine (5-EU) was purchased from Jena Bioscience (CLK-N002) and resuspended to an initial stock concentration of 400 mM in DMSO.
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bioRxiv - Molecular Biology 2021Quote: ... The 100% m6A modified full-length PAN transcript was synthesized using the MEGAscript T7 Transcription Kit following the manufacturer’s protocol but substituting adenosine-triphosphate with N6-methyladenosine-5’-triphosphate (Jena Bioscience NU-1101S). RNAs were purified using the MEGAclear Transcription Clean-Up Kit (ThermoFisher AM1908).
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bioRxiv - Microbiology 2020Quote: ... and expression of the host Leishmania tarentolae strain T7-TR were performed according to the Jena Bioscience protocol for inducible expression of recombinant proteins secreted to medium (LEXSinduce Expression kit, Jena Bioscience, Germany). Expression of proKLK13 and proKLK14 was induced with 15 µg/ml of tetracycline (BioShop ...
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bioRxiv - Immunology 2019Quote: ... suis proteins were amplified and cloned into the vector pLEXSYsat2 of the Leishmania Expression System (LEXSYcon2 Expression Kit, Jena Bioscience GmbH, Jena, Germany). The cloning vector was sequenced for verification ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 % CO2 for 1 h after a final concentration of 500 µM 5-Ethynyluridine (5EU, Jena Bioscience) was added ...
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bioRxiv - Microbiology 2019Quote: ... 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM AF647-Picolyl-azide (Jena Biosciences) and 10 mM sodium ascorbate (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... 2’-deoxythymidine-5’-[α-thio]-triphosphate (dTTPαS) and 2’-deoxyguanosine-5’-[α-thio]-triphosphate (dGTPαS) were obtained from Jena Biosciences. N-methylanthraniloyl guanosine-5’-triphosphate (mant-GTP ...
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bioRxiv - Cell Biology 2019Quote: ... nascent RNA was labelled by incubating the cells with 400 μM 5-ethynyluridine (5-EU; Jena Bioscience; CLK-N002-10,), which was then visualized with a click-iT mix consisting of 50mM Tris buffer pH8 ...
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bioRxiv - Molecular Biology 2022Quote: ... in the presence of 0.4μL 5-(3-Aminoallyl)-uridine-5’-triphosphate labeled with ATTO 680(Aminoallyl-UTP-ATTO-680, NU-821-680, Jena bioscience) in each reaction ...
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bioRxiv - Plant Biology 2019Quote: ... 5-Ethynyl-UTP was purchased from Jena Bioscience. A fraction of the reaction was analyzed using a standard agarose gel ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience) at 2 mM and MgCl2 at 4 mM was incubated for 15 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Immunostaining was performed in four steps to avoid unspecific binding of the secondary antibodies: i) incubation overnight at 4LJ with the mouse monoclonal IgG2a J2 antibody anti-dsRNA (Jena Bioscience, cat. RNT-SCI-10010500, dilution 1:200), ii ...
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bioRxiv - Biochemistry 2020Quote: ... 5’-guanylyl imidodiphosphate (GDPNP; Jena Bioscience NU-401-50) was added to the wells 16 minutes after the start of the reaction for five minutes at a concentration of 100uM followed by a five-minute pretreatment of either 208uM emetine (Cayman Chemical 21048 ...
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bioRxiv - Cell Biology 2022Quote: ... and 5 mM ATP (Jena Biosciences; NU-1010-SOL)) supplemented with 220 nM Sytox Orange at 100 μl/min ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 μM ppGpp (Guanosine-3’,5’-bisdiphosphate) (Jena Bioscience).
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bioRxiv - Immunology 2023Quote: ... 10 μM EdU (5-ethynyl-2’-deoxyuridine, Jena Bioscience) was added to the cell culture medium for 10 min at the end of 72 h culture on anti-CD3ε- and anti-CD28-coated cell culture plates ...
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bioRxiv - Microbiology 2023Quote: ... 5 µl biotin-11-CTP (1 mM) (Jena Bioscience), 0.8 µl DTT (0.5 M ...
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bioRxiv - Cell Biology 2023Quote: ... 5-fold molar excess of DBCO-AF647 (Jena Bioscience) was added to the azide-coupled Nb followed by incubation for 2 hours at room temperature and subsequently purified by dialysis and hydrophobic interaction chromatography (HIC).
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bioRxiv - Genomics 2023Quote: ... N1-Methylpseudouridine-5’-Triphosphate (m1meΨTP, Jena Bioscience, NU-890S) instead of UTP or N4-Acetyl-cytidine-5’-triphosphate (N4-Acetyl-CTP ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified with 4% Glutathione Agarose (Jena Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were purified by Ni2+-NTA chromatography (Jena Bioscience) and eluted stepwise in binding buffer with 40–250 mM imidazole ...
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bioRxiv - Zoology 2020Quote: ... in which a third of the dTTP had been replaced with Digoxigenin-X-(5-aminoallyl)-2’-deoxyuridine-5’-triphosphate (Jena Bioscience, Jena, Germany) for 40 cycles ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyguanosine-5’-triphosphate (3’-dGTP, Jena Bioscience) and 4 mM MgCl2 ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience), 4 mM MgCl2 and 2 μM G-actin ...
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bioRxiv - Neuroscience 2023Quote: ... 0,2 µM 5/6 TAMRA-PEG4-Alkyne tag (Jena Biosciences) and 0,2 mM freshly prepared CuSO4 ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM of m7GP3G monomethylated cap analogue (Jena Bioscience, NU-852) was soaked into mature NSP14 crystals (grown in 1.26 M sodium phosphate monobasic ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with 5’ethynyluridine (EU) (0.5 mM) (Jena Bioscience) and Click-iT Alexa Fluor 647 labelling kit (Thermofisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Extracted proteins were incubated overnight with Alkyne agarose beads (Jena Bioscience), and downstream preparations were done using Click Chemistry Capture Kit following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: Cells seeded in 6-well plates were treated with 20 μM OP-puro (Jena Bioscience) at 37°C for 30 min ...
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bioRxiv - Genomics 2022Quote: ... with 50% supplement of 5-Methyl-CTP and Pseudo-UTP (Jena Biosciences), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... and N1-methyladenoside 5’-phosphate (N1-methyl-AMP) was from Jena Bioscience. MTase-Glo™ Methyltransferase Assay Kit containing SAM and SAH were purchased from Promega ...
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bioRxiv - Cancer Biology 2023Quote: EDA-m7GTP-5-FAM (NU-824-5FM) was purchased from Jena Bioscience and used as received ...
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bioRxiv - Immunology 2023Quote: ... Cells were pulsed with OPP (20μM, Jena Bioscience, cat: NU-931-5) in complete RPMI supplemented with the cytoplasmic translation inhibitor harringtonine (1μM ...
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bioRxiv - Microbiology 2022Quote: ... Transformants were selected onto YPD agar plates containing 200 μg/mL nourseothricin (Jena Bioscience GmbH, Germany) and screened by colony PCR to confirm the presence of the mutation (Table S2) ...