Labshake search
Citations for Jena Bioscience :
1 - 50 of 70 citations for Recombinant Human IL6 Protein non tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
-
bioRxiv - Microbiology 2022Quote: ... Then GST-tagged proteins were pulled down using Glutathione magnetic agarose (Jena Bioscience). Beads were washed with protein binding buffer and proteins are then eluted using Laemmli buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... transfected cells were treated with 20 ng/ml recombinant human TNFα (Jena Biosciences) for 30 min ...
-
bioRxiv - Genomics 2022Quote: ... biotinylated dATP (Jena Bioscience) was used instead of dATP to increase ligation efficiency ...
-
bioRxiv - Immunology 2023Quote: ... biotinylated with desthiobiotin-dATP (Jena Bioscience) and 60 units DNA polymerase (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... biotinylated with desthiobiotin-ATP (Jena Bioscience) by 60 U Klenow fragment exo-(Thermo Scientific ...
-
bioRxiv - Genomics 2022Quote: ... and biotinylated dATP and dCTP (Jena Bioscience #NU-835-BIO14-S and #NU-809-BIOX-S ...
-
bioRxiv - Cell Biology 2023Quote: ... incorporating biotinylated UTP (Jena Bioscience, NU-821-BIO16). Biotinylated 3’ UTR was then incubated with cytoplasmic lysates in dialysis buffer (10 mM HEPES pH 7.4 ...
-
bioRxiv - Cancer Biology 2024Quote: ... 33uM biotinylated dUTP (Jena Bioscience, NU-803-BIOX), 1.5ul of Klenow Fragment (3’→5′ exo- ...
-
bioRxiv - Molecular Biology 2022Quote: ... Biotinylated cytidine (bis)phosphate (NU-1706-Bio, Jena Bioscience), Immun-Star AP substrate (1705018 ...
-
bioRxiv - Microbiology 2020Quote: ... and expression of the host Leishmania tarentolae strain T7-TR were performed according to the Jena Bioscience protocol for inducible expression of recombinant proteins secreted to medium (LEXSinduce Expression kit, Jena Bioscience, Germany). Expression of proKLK13 and proKLK14 was induced with 15 µg/ml of tetracycline (BioShop ...
-
bioRxiv - Microbiology 2020Quote: ... Another non-hydrolyzable analog CMP-PCP (Jena Bioscience) was unsuitable for our assays as Caulobacter ParB does not bind CMP-PCP (Figure 6-figure supplement 1).
-
bioRxiv - Microbiology 2023Quote: ... and 100 µM of non-hydrolysable UpNHpp (Jena Bioscience). The de novo reaction mix was incubated for 4 h at 30°C ...
-
bioRxiv - Genetics 2023Quote: ... The restriction overhangs were filled in using a biotinylated dATP (Jena Bioscience) and ligation was performed for 4 hours at 16°C (T4 DNA ligase ...
-
bioRxiv - Cell Biology 2022Quote: ... 7 μM of biotinylated tubulin and 0.5 mM GMPCPP (Jena Bioscience, NU-405S) in BRB80 buffer was incubated at 37°C for 40 min ...
-
bioRxiv - Biochemistry 2022Quote: ... NAD-capped RNA was then biotinylated (biotin-PEG3-azide, 250 mM, Jena Bioscience) via a copper-catalyzed azide-alkyne cycloaddition (CuAAC ...
-
bioRxiv - Cell Biology 2023Quote: The in vitro transcribed RNAs were 3’ biotinylated using pCp-Biotin (Jena Biosciences). Briefly ...
-
bioRxiv - Biochemistry 2023Quote: ... The pre-tRNAs were 3’ biotinylated with pCp-biotin (750 µM; Jena Bioscience) and T4 RNA ligase (2 units/µl) ...
-
bioRxiv - Molecular Biology 2024Quote: Recombinant PI3K-p85 regulatory subunit was obtained from Jena Bioscience GmbH (Jena ...
-
bioRxiv - Biochemistry 2020Quote: ... The non-hydrolysable analogs of aa-tRNAs were purchased from Jena Biosciences, Germany.
-
bioRxiv - Biochemistry 2022Quote: ... For loading of the non-hydrolyzable nucleotide analog mantGMPPNP (mGMPPNP, Jena Biosciences), KRAS protein was incubated with alkaline phosphatase beads (Sigma Aldrich ...
-
bioRxiv - Genomics 2020Quote: ... Digested fragments were labeled with biotinylated d-ATP from Jena Bioscience (NU-835-BIO14-S) and ligated ...
-
bioRxiv - Neuroscience 2023Quote: ... EPEA-displaying VLPs were first biotinylated using biotin-PEG4-NHS ester (Jena Bioscience, CLK-B103), after which it was immobilised on a 4PCP-STA streptavidin chip to a final density of 58 pg/mm2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... for GTP analog treated samples Non-hydrolyzable GTP Test Kit (Jena Bioscience #NK-102) containing 5 analogs – GTPαS ...
-
bioRxiv - Cancer Biology 2022Quote: ... the beads were treated with terminal deoxynucleotidyl transferase (EP0162) and biotinylated nucleotides (Biotin-11-dCTP, Jena Bioscience). The beads were then washed with IP buffer (50mM Tris–HCl pH 7.5 ...
-
bioRxiv - Immunology 2023Quote: ... 1 µg/ml of recombinant YF17D-DIII or TBEV-DIII (Jena Bioscience, PR-1450-S) was used ...
-
bioRxiv - Cell Biology 2021Quote: Microtubule seeds were prepared at 10 µM tubulin concentration (20 % ATTO-647-labelled tubulin and 80 % biotinylated tubulin) in BRB80 supplemented with 0.5 mM GMPCPP (Jena Bioscience) for 1 hour at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: Microtubule seeds were prepared at a final concentration of 10 µM tubulin (20 % ATTO-647-labelled tubulin and 80 % biotinylated tubulin) in BRB80 supplemented with 0.5 mM GMPCPP (Jena Bioscience) for 45 minutes at 37°C ...
-
bioRxiv - Genomics 2024Quote: ... and fragment ends filled-in and biotinylated by adding 27.5 µl of a cocktail containing 15 µL 1 mM biotin-14-dUTP (Jena BioScience), dCTP ...
-
bioRxiv - Microbiology 2020Quote: Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 μl of protein solution and 2 μl of crystallization mother liquor at 18°C ...
-
bioRxiv - Microbiology 2019Quote: Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 µl of protein solution and 2 µl of crystallization mother liquor at 18°C ...
-
bioRxiv - Genomics 2022Quote: ... human 22 with Atto-594-dUTPs and human 15 was labelled with Atto-425-dUTPs and Atto-594-dUTPs (Jena Bioscience). Hybridization was carried out overnight at 37°C ...
-
bioRxiv - Cell Biology 2019Quote: ... were generated by polymerization in the presence of the non-hydrolysable GTP analogue GMPCPP (NU-405S, Jena Bioscience) and then immobilized on coverslips incubated sequentially with the following solutions ...
-
bioRxiv - Cell Biology 2022Quote: ... Recombinant DFCP1 was loaded with nucleotides by incubation with either 400 µM ADP or ATPγS (Jena Bioscience) in exchange buffer (50 mM Tris ...
-
bioRxiv - Plant Biology 2023Quote: ... for degradation of bound GDP to GMP and Pi in the presence of 1.5-fold molar excess of non-hydrolyzable GTP analog GppCp (Jena Bioscience, Germany). After confirmation of GDP degradation by HPLC ...
-
bioRxiv - Biochemistry 2023Quote: Nucleotide binding was analyzed using the non-hydrolysable GTP analogue N- methylanthraniloyl guanosine-5’-[(β,γ)-imido]triphosphate (mant-GMPPNP, Jena Bioscience). Varying protein concentrations of non-farnesylated GBP1 ranging from 0.02 µM to 40 µM were prepared in a 96-well plate in 50 mM Tris-HCl pH 7.9 ...
-
bioRxiv - Biochemistry 2021Quote: ... recombinant full-length BGLF4 (EBV strain B95-8) was produced and purified from a commercially available Leishmania expression system (Jena Bioscience, Germany ...
-
bioRxiv - Biophysics 2020Quote: ... as a function of load in the presence of the non-hydrolyzable ATP analog adenosine-5’-[(β, γ)-imido] triphosphate (AMPPNP, Jena Bioscience). With AMPPNP ...
-
bioRxiv - Cell Biology 2021Quote: ... Biotin and Alexa-Fluor-633-labeled porcine tubulin were generated in the laboratory as described (Al-Bassam, 2014) and were polymerized using the non-hydrolysable GTP analog guanosine-5’-[(α,β)-methyleno] triphosphate (termed GMPCPP; Jena Biosciences) or using the MT stabilizing drug ...
-
bioRxiv - Biophysics 2019Quote: ... Biotin and Alexa-Fluor-633-labeled porcine tubulin were generated in the laboratory as described (Al-Bassam, 2014) and were polymerized using the non-hydrolyzable GTP analog guanosine-5’-[(α,β)-methyleno] triphosphate (termed GMPCPP; Jena Biosciences) or using the MT stabilizing drug ...
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified with 4% Glutathione Agarose (Jena Biosciences) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were purified by Ni2+-NTA chromatography (Jena Bioscience) and eluted stepwise in binding buffer with 40–250 mM imidazole ...
-
bioRxiv - Molecular Biology 2021Quote: ... Extracted proteins were incubated overnight with Alkyne agarose beads (Jena Bioscience), and downstream preparations were done using Click Chemistry Capture Kit following the manufacturer’s guidelines ...
-
bioRxiv - Biophysics 2019Quote: ... Control reactions for azide-bearing proteins were performed using TAMRA-DBCO (Jena Bioscience). Reaction mixtures were incubated for varying durations (0.5 hours to overnight ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cap-bound proteins were pulled down using γ-Aminophenyl-m7GTP beads (Jena Bioscience).
-
bioRxiv - Neuroscience 2023Quote: ... and 32μg of protein was clicked to 31μM biotin-alkyne (Jena biosciences, #CLK-TA105) mixed in PBS pH 7.8-PI (Calbiochem ...
-
bioRxiv - Biophysics 2021Quote: ... The protein was incubated with ~20× molar excess of Dibenzylcyclooctyne(DBCO)-Sulfo-Cy5 (Jena Bioscience) at 4°C overnight in the dark ...
-
bioRxiv - Cancer Biology 2020Quote: ... Proteins were then extracted and probes were clicked onto azide-functionalized magnetic beads (Jena Bioscience) as described above ...
-
bioRxiv - Cell Biology 2021Quote: ... Total proteins (500μg) were incubated with 15μL Immobilized γ-Aminophenyl-m7GTP (Jena Bioscience, #AC-155) at 4°C for 3 hours ...
-
bioRxiv - Molecular Biology 2022Quote: ... Quantitative protein occupancy was performed by qPCR according to the manufacturer’s instructions (Jena Bioscience, PCR-369). Mean values for the analyzed regions were expressed as fold enrichment compared to a negative control without protein occupation (SOX2 gene) ...