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Citations for Jena Bioscience :
1 - 50 of 52 citations for Recombinant Human Carboxylesterase 3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
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bioRxiv - Biochemistry 2020Quote: ... transfected cells were treated with 20 ng/ml recombinant human TNFα (Jena Biosciences) for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... His-Cdc42 was instead dialyzed with 20 µM GppNHp (Jena Bioscience) in the presence of 5 U of Quick-CIP alkaline phosphatase (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... Then GST-tagged proteins were pulled down using Glutathione magnetic agarose (Jena Bioscience). Beads were washed with protein binding buffer and proteins are then eluted using Laemmli buffer ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience) at 2 mM and MgCl2 at 4 mM was incubated for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: Recombinant PI3K-p85 regulatory subunit was obtained from Jena Bioscience GmbH (Jena ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3’deoxy GTP (3′dGTP) from Jena Bioscience.
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyguanosine-5’-triphosphate (3’-dGTP, Jena Bioscience) and 4 mM MgCl2 ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience), 4 mM MgCl2 and 2 μM G-actin ...
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bioRxiv - Immunology 2023Quote: ... 1 µg/ml of recombinant YF17D-DIII or TBEV-DIII (Jena Bioscience, PR-1450-S) was used ...
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bioRxiv - Microbiology 2020Quote: Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 μl of protein solution and 2 μl of crystallization mother liquor at 18°C ...
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bioRxiv - Microbiology 2019Quote: Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 µl of protein solution and 2 µl of crystallization mother liquor at 18°C ...
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bioRxiv - Genomics 2022Quote: ... human 22 with Atto-594-dUTPs and human 15 was labelled with Atto-425-dUTPs and Atto-594-dUTPs (Jena Bioscience). Hybridization was carried out overnight at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant DFCP1 was loaded with nucleotides by incubation with either 400 µM ADP or ATPγS (Jena Bioscience) in exchange buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... ≥95% purity for 3’-dGTP (Jena Bioscience)] and incubated 2 min at 37°C (to “chase,” and thereby to stabilize ...
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bioRxiv - Molecular Biology 2022Quote: ... ≥95% purity for 3’-dGTP (Jena Bioscience)] and incubated 2 min at 37°C (to “chase” non-terminated complexes) ...
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bioRxiv - Cell Biology 2023Quote: ... 3 µM Picolyl-Alexa647-Azide (Jena Bioscience) and 80 µM ascorbic acid (Merck) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were labeled at the 3’ end using pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl) phosphate) (Jena Biosciences). For each binding experiment ...
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bioRxiv - Biophysics 2023Quote: ... The final gene construct was completed from the 3’-terminus by KpnI restriction site followed by 9×His-tag nucleotide sequence and inserted into the integrative inducible expression vector pLEXSY_I-blecherry3 (Jena Bioscience, Germany) via the BglII(5’ ...
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bioRxiv - Biophysics 2020Quote: ... of λ-DNA using the following primers: 5’-CGAACTCTTCAAATTCTTCTTCCA-3’ and 5’-GATTGCTCTTCTGTAAGGTTTTG-3’ with a 5:1 ratio of dTTP:biotin-11-dUTP (Jena Bioscience). Similarly ...
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bioRxiv - Biophysics 2020Quote: ... 5’-TAGTCCAGAACGAGACCGCAACAGCACAACCCAAACTG-3’ and 5’-AATCTGCTGCAATGCCACAG-3’ (underline section indicates overhang) with a 10:1 ratio of dTTP:digoxigenin-11-dUTP (Jena Bioscience). The labelled fragments were purified using a PCR purification kit (QIAquick ...
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bioRxiv - Biophysics 2020Quote: ... and 3 mM GMPCPP (NU-405S, Jena Bioscience) was mixed on ice ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... and 3-azido-7-hydroxycoumarin (Jena Bioscience, CLK-FA047). LD540 dye was a kind gift from Christoph Thiele (University of Bonn ...
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bioRxiv - Biophysics 2019Quote: ... 2’-(3’)-bis-O-(N-methylanthraniloyl)-GDP (Jena Biosciences). Rab5 was loaded with mant-GDP in Rab5 buffer + 10mM EDTA and 5-fold excess mant-GDP ...
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bioRxiv - Biochemistry 2019Quote: ... 3’ end-labelled using Aminoallyl-UTP-Cy5 (Jena Bioscience) incubated with TdT.
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bioRxiv - Molecular Biology 2023Quote: ... 250 μM ppGpp (Guanosine-3’,5’-bisdiphosphate) (Jena Bioscience).
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bioRxiv - Genetics 2019Quote: ... EdU was coupled to a cleavable biotin-azide linker (Azide-PEG(3+3)-S-S-biotin) (Jena Biosciences, Cat. No. CLK-A2112-10) using the reagents of the Click-it Kit (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant full-length BGLF4 (EBV strain B95-8) was produced and purified from a commercially available Leishmania expression system (Jena Bioscience, Germany ...
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bioRxiv - Microbiology 2020Quote: ... and expression of the host Leishmania tarentolae strain T7-TR were performed according to the Jena Bioscience protocol for inducible expression of recombinant proteins secreted to medium (LEXSinduce Expression kit, Jena Bioscience, Germany). Expression of proKLK13 and proKLK14 was induced with 15 µg/ml of tetracycline (BioShop ...
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bioRxiv - Genomics 2023Quote: 3’-(O-Propargyl)-ATP (NU-945) was offered by Jena Biosciences. To complete the set ...
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bioRxiv - Biophysics 2019Quote: ... Cy3 labeled 2’/3’-O-(2-aminoethyl-carbamoyl)-adenosine-5’-triphosphate (Jena Bioscience) was added into the imaging buffer instead of ATP.
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bioRxiv - Biochemistry 2021Quote: ... For labeling pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl)phosphate (Jena bioscience), was ligated to the 3’ end of the 16 nt RNA using T4 RNA ligase (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: The in vitro transcribed RNAs were 3’ biotinylated using pCp-Biotin (Jena Biosciences). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... The pre-tRNAs were 3’ biotinylated with pCp-biotin (750 µM; Jena Bioscience) and T4 RNA ligase (2 units/µl) ...
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bioRxiv - Biochemistry 2022Quote: ... To assemble microtubules stabilized with guanylyl-(a,(3)-methylene-diphosphonate (GMP-CPP) (Jena Bioscience), tubulin was diluted to 2 mg/mL in BRB80 buffer supplemented with 0.5 mM GMP-CPP ...
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bioRxiv - Genomics 2020Quote: ... Female genomic probe was labelled with Cy3-dUTP (cyanine 3-deoxyuridine triphosphate; Jena Bioscience, Germany) by nick translation ...
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bioRxiv - Microbiology 2022Quote: ... and 1 μL of 3-azido-7-hydroxycoumarin solution (1 mM in EtOH; Jena Bioscience) was added ...
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Tail Length and E525K Dilated Cardiomyopathy Mutant Alter Human β-Cardiac Myosin Super-Relaxed StatebioRxiv - Molecular Biology 2023Quote: The fluorescence of 3’-O-(N-Methyl-anthraniloyl)-2’-deoxyadenosine-5’-triphosphate (mantATP) (Jena Biosciences) was measured with 290 nm excitation and a 395 nm long-pass emission filter in a stopped-flow apparatus (Applied Photophysics ...
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bioRxiv - Biophysics 2024Quote: ... a 3:1 BrdU/BrdC mix (Sigma-Aldrich, #B5002, and Jena Bioscience, #N-DN-6496) was added to the cells ...
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bioRxiv - Biochemistry 2020Quote: ... 3’dGTP and cytidine-5’-[(α,β)-methyleno]triphosphate (CMPCPP) were from Jena Bioscience (Jena, Germany); 2’dGTP ...
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bioRxiv - Immunology 2021Quote: ... XCL1-K(N3) and sdAb-K(N3) were subsequently reacted with DBCO-Cy5.5 (3 equiv.) (CLK-1046, Jena BioSciences) overnight at RT on an end-over-end shaker ...
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bioRxiv - Genetics 2021Quote: ... the PCR labelling Cy-3 and Cy-5 kits (Jena Bioscience, ref# PP-301L-Cy3 and #APP-101-Cy5) according to the manufacturer’s instructions with M13 universal primers ...
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bioRxiv - Biochemistry 2020Quote: Guanosine nucleotides labeled with fluorescent N-methyl-anthranoyl (mant) groups O-linked with 2’ or 3’ of ribose (Jena Biosciences), were used for binding studies ...
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bioRxiv - Biochemistry 2021Quote: ... RNAs were fluorescently labeled with cyanine 5 at the 3’ end with the help of pCp-Cy5 (Jena Bioscience, 84179) and T4 RNA ligase I (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... in the presence of 0.4μL 5-(3-Aminoallyl)-uridine-5’-triphosphate labeled with ATTO 680(Aminoallyl-UTP-ATTO-680, NU-821-680, Jena bioscience) in each reaction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... In-vitro transcription was conducted with HighYield T7 Cap 1 AG (3‘-OMe) mRNA Synthesis Kit (m5CTP) (Jena Bioscience, Germany) using 800 ng of amplified template followed by Turbo DNase treatment (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: T7 promoter containing double stranded DNA (Supplementary Table 3) was used as a template for in vitro transcription with HighYield T7 mRNA Synthesis Kit (ac4CTP) (Jena Bioscience).
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Dried lipid films were click reacted in a 40 μl reaction mix containing 0.45 mM fluorogenic dye 3-azido-7-hydroxycoumarin (Jena Bioscience, CLK-FA047), 1.4 mM Cu(I)tetra(acetonitrile ...
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bioRxiv - Biochemistry 2022Quote: Guanine nucleotide exchange activity was measured by following changes in the fluorescence of the GDP derivative mant-dGDP [2’-Deoxy-3’-O-(N-Methyl-anthraniloyl) guanosine-5’-diphosphate sodium salt (Jena Bioscience GmbH)] (Margarit et al. ...
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bioRxiv - Evolutionary Biology 2024Quote: ... strains with MYO3 or MYO5 locus fused at its 3’ to the DHFR F[1,2] with a nourseothricin-resistance marker (NAT 100 μg/ml, Jena Bioscience GmbH, Germany). To insert the variant libraries instead of the wild-type SH3 domains ...