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Citations for Jena Bioscience :
1 - 50 of 133 citations for Recombinant Human Aldehyde Dehydrogenase 1 Family Member A3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
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bioRxiv - Biochemistry 2020Quote: ... transfected cells were treated with 20 ng/ml recombinant human TNFα (Jena Biosciences) for 30 min ...
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bioRxiv - Immunology 2023Quote: ... 1 µg/ml of recombinant YF17D-DIII or TBEV-DIII (Jena Bioscience, PR-1450-S) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... His-Cdc42 was instead dialyzed with 20 µM GppNHp (Jena Bioscience) in the presence of 5 U of Quick-CIP alkaline phosphatase (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... Then GST-tagged proteins were pulled down using Glutathione magnetic agarose (Jena Bioscience). Beads were washed with protein binding buffer and proteins are then eluted using Laemmli buffer ...
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bioRxiv - Molecular Biology 2024Quote: Recombinant PI3K-p85 regulatory subunit was obtained from Jena Bioscience GmbH (Jena ...
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bioRxiv - Microbiology 2020Quote: Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 μl of protein solution and 2 μl of crystallization mother liquor at 18°C ...
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bioRxiv - Microbiology 2019Quote: Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 µl of protein solution and 2 µl of crystallization mother liquor at 18°C ...
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bioRxiv - Genomics 2022Quote: ... human 22 with Atto-594-dUTPs and human 15 was labelled with Atto-425-dUTPs and Atto-594-dUTPs (Jena Bioscience). Hybridization was carried out overnight at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant DFCP1 was loaded with nucleotides by incubation with either 400 µM ADP or ATPγS (Jena Bioscience) in exchange buffer (50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... The final gene construct was completed from the 3’-terminus by KpnI restriction site followed by 9×His-tag nucleotide sequence and inserted into the integrative inducible expression vector pLEXSY_I-blecherry3 (Jena Bioscience, Germany) via the BglII(5’ ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant full-length BGLF4 (EBV strain B95-8) was produced and purified from a commercially available Leishmania expression system (Jena Bioscience, Germany ...
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bioRxiv - Microbiology 2020Quote: ... and expression of the host Leishmania tarentolae strain T7-TR were performed according to the Jena Bioscience protocol for inducible expression of recombinant proteins secreted to medium (LEXSinduce Expression kit, Jena Bioscience, Germany). Expression of proKLK13 and proKLK14 was induced with 15 µg/ml of tetracycline (BioShop ...
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bioRxiv - Biochemistry 2023Quote: ... 0.9 mL of DNase solution (15 mM MgSO4, 1 mM DTT and 1 mM PMSF, 1 x Turbo DNase (Jena biosciences)) was added and the samples were allowed to shake at 37 °C for 10 min before transferring to ice ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with SiR-Tet (1–2 µM, 1 h; Spirochrome, Stein am Rhein, Switzerland) or TAMRA-Tet (2 µM, 1 h; Jena BioScience, Germany) and washed again with fresh medium (3 × quick wash and 3 × 30 min wash ...
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bioRxiv - Cell Biology 2019Quote: ... 12 μM unlabeled tubulin + 1 μM Alexa-568 tubulin + 1 μM biotin tubulin was polymerized in BRB80 (80 mM Pipes, 1 mM EGTA, 1 mM MgCl2) in the presence of 1 mM GMPCPP (Jena Bioscience, NU-405L) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... as in 74 from a mixture of unlabeled, rhodamine- and biotin-labeled tubulins (8:1:1, total tubulin concentration 5 μM) supplemented with 1 mM GMPCPP (Jena Bioscience, NU-405L). A flow chamber was prepared as for assays with taxol-stabilized microtubules ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM GTP (Roth, K056.4) or 1 mM GMP (Jena Biosciences, JBS-NU-1028), was added to the washed beads and incubated gently by rocking for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM GMPCPP (Jena Biosciences) and 4 mM MgCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18.000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... and 1 μL of 3-azido-7-hydroxycoumarin solution (1 mM in EtOH; Jena Bioscience) was added ...
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bioRxiv - Neuroscience 2024Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GTP (Jena Bioscience, Jena, Germany) for 30 minutes at 37 °C prior to centrifugation at 18.000 x g for 30 min in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Biophysics 2021Quote: ... 1 mM GMPCPP (Jena Bioscience, Germany), 1 mM MgCl2 and 4 mg mL-1 of porcine brain tubulin ...
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bioRxiv - Cell Biology 2021Quote: ... with 1 mM GMPCPP (Jena Bioscience) in PEM80 (80 mM Pipes ...
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bioRxiv - Biophysics 2023Quote: ... and Wizard 1&2 (Jena Bioscience) screens were used at 16 °C ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 mM GTP (Jena Bioscience). A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... AF488-Azide (#CLK-1275-1, Jena Bioscience) was used to test success of bead – DBCO coupling ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM GMPPCP (NU-405S, Jena Bioscience) or 1 mM BeF4 (prepared by mixing 50 mM BeSO4 and NaF 100 mM to make 10 mM BeF3) ...
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bioRxiv - Microbiology 2021Quote: ... Labeling was achieved by using a 1:1 molar ratio of dCTP:Biotin-11-dCTP (NU-809-BIOX, Jena Bioscience) in the dNTPs used for the PCR (GoTaq DNA polymerase ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were resuspended using 1/5 or 1/10 dilutions of detergents stocks from the JBScreen (Jena Bioscience) or Detergent Screen (Hampton) ...
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bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C in click reagents (1:1 DMSO/H2O with 20 μM picolyl-azide-sulfo-Cy5 (Jena Bioscience), 300 μM CuSO4 ...
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bioRxiv - Biochemistry 2020Quote: ... JBScreen Classic 1-4 and 6 (Jena Bioscience) and Crystal Screen ...
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bioRxiv - Cell Biology 2022Quote: ... was incubated with 1 mM GMPCPP (Jena Biosciences) at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and 50 µg mL-1 nourseothricin (Jena Bioscience).
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bioRxiv - Microbiology 2023Quote: ... JBScreen Classic 1-4 and 6 (Jena Bioscience) and Crystal Screen ...
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bioRxiv - Cell Biology 2024Quote: ... was incubated with 1 mM GMPCPP (Jena Biosciences) at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The assay was carried out at 37°C and the reaction was started by addition of 1 mM ATP and 1 mM GTP (Jena Bioscience), and samples for the nucleotide measurement were taken after 15 min ...
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bioRxiv - Immunology 2023Quote: ... and first PCR reaction was performed on 1 µl of a 1 in 100 diluted freeze-thawed cell pellet using the SCRIPT HF RT-PCR Kit (Jena Bioscience), according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... 2 mg mL−1 BSA (Jena Bioscience, BU-102) in H2O) ...
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bioRxiv - Genomics 2020Quote: ... anchored oligo-dT primer (1 µM, TTTTTTTTTTTTTTTTTTTTVN, Jena Bioscience), buffer ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM Mg-GTP or Mg-GMPCPP (Jena Biosciences), and an oxygen scavenging system (80 μg/mL Catalase (Sigma C1345-1G) ...
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bioRxiv - Biophysics 2022Quote: Cy3ATP was purchased from Jena Bioscience (1 mM stock). The 2’-deoxy-ATP labeled with N-Methylanthraniloyl at the 3’-ribose position (mantATP ...
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bioRxiv - Biophysics 2022Quote: ... 1.5-1.8 µL 1 mM AF647-dUTP (Jena BioScience) and 0.75 µL 25 µM LacI-AF488 and incubated in the flow channel for between 10 and 15 min ...
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bioRxiv - Plant Biology 2023Quote: ... or 100 μg mL−1 nourseothricin (Jena Bioscience, Germany).
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bioRxiv - Microbiology 2023Quote: ... 5 µl biotin-11-CTP (1 mM) (Jena Bioscience), 0.8 µl DTT (0.5 M ...
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bioRxiv - Cell Biology 2020Quote: ... from a mixture of unlabeled and HiLyte647-labeled tubulin (5:1, total tubulin concentration 100 μM) and 1 mM GMPCPP (Jena Bioscience, Jena, Germany) incubated at 37°C for 20 min ...
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bioRxiv - Developmental Biology 2021Quote: CRISPR-UMI cassettes were PCR amplified and multiplexed with 25 cycles of 1:1 KlenTag/Phusion PCR (Jena Bioscience and in house generated) using primers specified in (Michlits et al ...
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bioRxiv - Cell Biology 2019Quote: ... Two crystallization screens were used JBScreen Basic 1 (Jena Bioscience) and JCSG plus (Molecular Dimensions) ...
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bioRxiv - Biochemistry 2022Quote: ... 150 mM KCl buffer containing 1 mM GTP (Jena Bioscience) and 1 mM MgCl2 and the released inorganic phosphate was measured using a malachite green-based colorimetric assay (Baykov et al. ...