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Citations for Jena Bioscience :
1 - 50 of 54 citations for Recombinant Cyan Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... Then GST-tagged proteins were pulled down using Glutathione magnetic agarose (Jena Bioscience). Beads were washed with protein binding buffer and proteins are then eluted using Laemmli buffer ...
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bioRxiv - Microbiology 2020Quote: ... and expression of the host Leishmania tarentolae strain T7-TR were performed according to the Jena Bioscience protocol for inducible expression of recombinant proteins secreted to medium (LEXSinduce Expression kit, Jena Bioscience, Germany). Expression of proKLK13 and proKLK14 was induced with 15 µg/ml of tetracycline (BioShop ...
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bioRxiv - Genomics 2021Quote: ... a fluorescent ATTO-550-UTP (Jena biosciences GMBH) in the same quantity was added in the nucleotide mixture ...
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bioRxiv - Molecular Biology 2021Quote: ... His-Cdc42 was instead dialyzed with 20 µM GppNHp (Jena Bioscience) in the presence of 5 U of Quick-CIP alkaline phosphatase (New England Biolabs) ...
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bioRxiv - Biochemistry 2021Quote: ... or fluorescent trinitrophenyl adenosine triphosphate (TNP-ATP, Jena Bioscience) were added to the bath solution to assess current inhibition and/or nucleotide binding ...
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bioRxiv - Molecular Biology 2024Quote: Recombinant PI3K-p85 regulatory subunit was obtained from Jena Bioscience GmbH (Jena ...
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bioRxiv - Molecular Biology 2022Quote: ... fluorescent puromycin (6-FAM-dC-Puromycin, Jena Bioscience #NU-925-6FM-L) was added to 4.6 µM ...
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bioRxiv - Biochemistry 2020Quote: ... transfected cells were treated with 20 ng/ml recombinant human TNFα (Jena Biosciences) for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... E60AzF and E143AzF mutants were labeled with AF647 fluorescent dye (Jena Bioscience, Jena, Germany) by mixing the protein with 5x molar excess of DBCO-AF647 ...
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bioRxiv - Immunology 2023Quote: ... 1 µg/ml of recombinant YF17D-DIII or TBEV-DIII (Jena Bioscience, PR-1450-S) was used ...
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bioRxiv - Microbiology 2020Quote: Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 μl of protein solution and 2 μl of crystallization mother liquor at 18°C ...
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bioRxiv - Microbiology 2019Quote: Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 µl of protein solution and 2 µl of crystallization mother liquor at 18°C ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant DFCP1 was loaded with nucleotides by incubation with either 400 µM ADP or ATPγS (Jena Bioscience) in exchange buffer (50 mM Tris ...
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bioRxiv - Plant Biology 2019Quote: Fluorescent labeling of dsRNA was performed using the Atto 488 RNA Labeling Kit (Jena Bioscience, Jena, Germany) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... TTN5T30N and TTN5Q70L were prepared by incubation of nucleotide-free forms with fluorescent nucleotides (Jena Bioscience, Germany) in a molar ratio of 1 to 1.2 ...
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bioRxiv - Biophysics 2023Quote: ... The final gene construct was completed from the 3’-terminus by KpnI restriction site followed by 9×His-tag nucleotide sequence and inserted into the integrative inducible expression vector pLEXSY_I-blecherry3 (Jena Bioscience, Germany) via the BglII(5’ ...
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bioRxiv - Plant Biology 2023Quote: ... 2005) with the corresponding NBD(NitroBenzoxadiazol)- modified fluorescent prenyl substrates (respectively NBD-FPP or NBD-GGPP, Jena Biosciences). GSTs equipped with a farnesylatable CAIM (GST-CAIM ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μl of 40 μM PE* primer and 0.8 μl of 100 μM EvaGreen Fluorescent DNA Stain (Jena Bioscience). The reactions were amplified in a CFX96 Real-Time PCR instrument (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: Nucleotide release and binding measurements were performed using the fluorescent nucleotide analogues MABA-ADP (Jena Bioscience, NU-893-MNT) and MABA-ATP (Jena Bioscience ...
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bioRxiv - Biochemistry 2020Quote: Guanosine nucleotides labeled with fluorescent N-methyl-anthranoyl (mant) groups O-linked with 2’ or 3’ of ribose (Jena Biosciences), were used for binding studies ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant full-length BGLF4 (EBV strain B95-8) was produced and purified from a commercially available Leishmania expression system (Jena Bioscience, Germany ...
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bioRxiv - Plant Biology 2022Quote: ... Purified PCR products and plasmid DNAs were labeled with ATTO488-dUTP or ATTO550-dUTP using Fluorescent Nick Translation Labeling kits (Jena Bioscience, Germany).
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bioRxiv - Biophysics 2021Quote: ... we labeled the vimentin monomers with the fluorescent dye ATTO647N (AD 647N-41, AttoTech, Siegen, Germany) and with biotin via malemeide (B1267-25MG, Jena Bioscience, Jena, Germany) as described in Refs ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified with 4% Glutathione Agarose (Jena Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were purified by Ni2+-NTA chromatography (Jena Bioscience) and eluted stepwise in binding buffer with 40–250 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... Extracted proteins were incubated overnight with Alkyne agarose beads (Jena Bioscience), and downstream preparations were done using Click Chemistry Capture Kit following the manufacturer’s guidelines ...
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bioRxiv - Biophysics 2019Quote: ... Control reactions for azide-bearing proteins were performed using TAMRA-DBCO (Jena Bioscience). Reaction mixtures were incubated for varying durations (0.5 hours to overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Cap-bound proteins were pulled down using γ-Aminophenyl-m7GTP beads (Jena Bioscience).
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bioRxiv - Neuroscience 2023Quote: ... and 32μg of protein was clicked to 31μM biotin-alkyne (Jena biosciences, #CLK-TA105) mixed in PBS pH 7.8-PI (Calbiochem ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated with ~20× molar excess of Dibenzylcyclooctyne(DBCO)-Sulfo-Cy5 (Jena Bioscience) at 4°C overnight in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were then extracted and probes were clicked onto azide-functionalized magnetic beads (Jena Bioscience) as described above ...
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bioRxiv - Cell Biology 2021Quote: ... Total proteins (500μg) were incubated with 15μL Immobilized γ-Aminophenyl-m7GTP (Jena Bioscience, #AC-155) at 4°C for 3 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative protein occupancy was performed by qPCR according to the manufacturer’s instructions (Jena Bioscience, PCR-369). Mean values for the analyzed regions were expressed as fold enrichment compared to a negative control without protein occupation (SOX2 gene) ...
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bioRxiv - Developmental Biology 2020Quote: ... 250 µg of protein was pre-cleared using 50 µL of agarose beads (Jena Biosciences, AC-100S) in Cap Lysis Buffer for 30 min at 4°C with rocking ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alkyne labelled proteins were enriched and affinity purified by direct attachment to azide agarose beads (Jena Bioscience) using copper catalysed click chemistry ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 μg of protein was incubated overnight at 4°C with m7GTP agarose beads (Jena biosciences, #AC-155S). Beads were washed with cap binding buffer and suspended in 20 μL Laemmli buffer ...
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bioRxiv - Molecular Biology 2022Quote: C15-az and C17-az modified proteins were pulled down using the Click Chemistry Capture Kit (Jena Bioscience) and alkyne agarose beads (Jena Bioscience) ...
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bioRxiv - Microbiology 2023Quote: ... Eluted protein was incubated with an ATP-agarose resin (Immobilized γ-Aminophenyl-ATP C10-spacer from Jena Bioscience) overnight at 4 °C with gentle agitation ...
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bioRxiv - Microbiology 2021Quote: ... crescentus ParBΔCTD-CTPɣS complex crystal were also set up in sitting-drop vapour diffusion format in MRC2 96-well crystallization plates with drops comprised of 0.3 µL precipitant solution and 0.3 µL of protein solution (∼10 mg/mL) supplemented with 1 mM CTPɣS (Jena Biosciences) and 1 mM MgCl2 ...
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bioRxiv - Microbiology 2021Quote: To verify the functionality of mutated SoGGDEF proteins they were tested for GTP binding using MANT-GTP (Jena Bioscience, Germany). Proteins were diluted to 1 μM and 100 μl of each sample was added in triplicates into a white round bottom 96-well plate before MANT-GTP with a final concentration of 1 μM was added ...
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bioRxiv - Neuroscience 2022Quote: ... 250-300 μg of protein lysate together with 30 μl of 7-methyl GTP-agarose beads (Jena bioscience #AC-141) were incubated for 90 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... expression of protein was induced in 45 mL BHI medium supplemented with bleomycin and tetracyclin 10 mg/mL (Jena Bioscience). 5 ml of culture from nonclonal selection was added incubated for 72 h at 26°C with shaking at 100 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 20 μl of the prepared protein were mixed with 5 μl of 500-fold diluted JBS Thermofluor Dye (Jena Bioscience), prepared in the same buffer ...
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bioRxiv - Immunology 2020Quote: ... purified naïve C57BL/6 B2 cells were preincubated with a mixture of NIP-OVA and HIV-1 p17/p24/gp120 fusion protein (Jena Biosciences) covalently bound to 1 μm beads (1:1 bead:Bcell ratio ...
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bioRxiv - Molecular Biology 2019Quote: ... Capturing of ATP binding proteins was performed on 75 μl of this mixture using 30 μl of Aminophenyl-ATP- or naked agarose beads (Jena Bioscience) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... GMPPNP binding Irgb6 crystals diffracting to 1.6 Å resolution were obtained from sitting drops with a 9 mg/ml protein solution containing 2 mM GMPPNP (Jena Bioscience) and a reservoir solution consisting of 0.1 M Sodium citrate buffer pH 5.6 ...
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bioRxiv - Physiology 2022Quote: ... glycoproteins were enriched using Concanavalin A and azido-modifed proteins were immobilized on magnetic DBCO-beads (Jena Bioscience #CLK-1037-1) via click chemistry ...
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bioRxiv - Microbiology 2023Quote: ... Protein-containing fractions were pooled and incubated with an ATP-agarose resin (Immobilized γ-Aminophenyl-ATP C10-spacer from Jena Bioscience) overnight at 4 °C with gentle agitation ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were measured using BCA and 300 µg of protein lysate was added to 50 µl of m7GTP beads (Jena Bioscience) for 2h at 4°C ...