Labshake search
Citations for Jena Bioscience :
51 - 100 of 225 citations for Rat Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... The protein was incubated with ~20× molar excess of Dibenzylcyclooctyne(DBCO)-Sulfo-Cy5 (Jena Bioscience) at 4°C overnight in the dark ...
-
bioRxiv - Cancer Biology 2020Quote: ... Proteins were then extracted and probes were clicked onto azide-functionalized magnetic beads (Jena Bioscience) as described above ...
-
bioRxiv - Cell Biology 2021Quote: ... Total proteins (500μg) were incubated with 15μL Immobilized γ-Aminophenyl-m7GTP (Jena Bioscience, #AC-155) at 4°C for 3 hours ...
-
bioRxiv - Molecular Biology 2022Quote: ... Quantitative protein occupancy was performed by qPCR according to the manufacturer’s instructions (Jena Bioscience, PCR-369). Mean values for the analyzed regions were expressed as fold enrichment compared to a negative control without protein occupation (SOX2 gene) ...
-
bioRxiv - Developmental Biology 2020Quote: ... 250 µg of protein was pre-cleared using 50 µL of agarose beads (Jena Biosciences, AC-100S) in Cap Lysis Buffer for 30 min at 4°C with rocking ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alkyne labelled proteins were enriched and affinity purified by direct attachment to azide agarose beads (Jena Bioscience) using copper catalysed click chemistry ...
-
bioRxiv - Molecular Biology 2021Quote: ... 500 μg of protein was incubated overnight at 4°C with m7GTP agarose beads (Jena biosciences, #AC-155S). Beads were washed with cap binding buffer and suspended in 20 μL Laemmli buffer ...
-
bioRxiv - Microbiology 2023Quote: ... Eluted protein was incubated with an ATP-agarose resin (Immobilized γ-Aminophenyl-ATP C10-spacer from Jena Bioscience) overnight at 4 °C with gentle agitation ...
-
bioRxiv - Cell Biology 2022Quote: ... Total RNA Purification kit was from Jena Biosciences, Jena ...
-
bioRxiv - Cancer Biology 2021Quote: ... or the Digoxigenin NT Labeling Kit (Jena Bioscience). Hybridization was carried out in a humidified chamber at 37°C for 16 h ...
-
bioRxiv - Microbiology 2020Quote: ... ATP Affinity kit (no. AK-102, Jena Biosciences).
-
bioRxiv - Biochemistry 2023Quote: ... 0.9 mL of DNase solution (15 mM MgSO4, 1 mM DTT and 1 mM PMSF, 1 x Turbo DNase (Jena biosciences)) was added and the samples were allowed to shake at 37 °C for 10 min before transferring to ice ...
-
bioRxiv - Molecular Biology 2022Quote: ... the qPCR Green Core kit (Jena Biosciences, PCR-333L) or the GoTaq® qPCR kit (Promega ...
-
bioRxiv - Neuroscience 2022Quote: ... 250-300 μg of protein lysate together with 30 μl of 7-methyl GTP-agarose beads (Jena bioscience #AC-141) were incubated for 90 min at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... expression of protein was induced in 45 mL BHI medium supplemented with bleomycin and tetracyclin 10 mg/mL (Jena Bioscience). 5 ml of culture from nonclonal selection was added incubated for 72 h at 26°C with shaking at 100 rpm ...
-
bioRxiv - Microbiology 2023Quote: ... 20 μl of the prepared protein were mixed with 5 μl of 500-fold diluted JBS Thermofluor Dye (Jena Bioscience), prepared in the same buffer ...
-
bioRxiv - Cell Biology 2019Quote: ... incubated with SiR-Tet (1–2 µM, 1 h; Spirochrome, Stein am Rhein, Switzerland) or TAMRA-Tet (2 µM, 1 h; Jena BioScience, Germany) and washed again with fresh medium (3 × quick wash and 3 × 30 min wash ...
-
bioRxiv - Cell Biology 2019Quote: ... 12 μM unlabeled tubulin + 1 μM Alexa-568 tubulin + 1 μM biotin tubulin was polymerized in BRB80 (80 mM Pipes, 1 mM EGTA, 1 mM MgCl2) in the presence of 1 mM GMPCPP (Jena Bioscience, NU-405L) for 1 h at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... as in 74 from a mixture of unlabeled, rhodamine- and biotin-labeled tubulins (8:1:1, total tubulin concentration 5 μM) supplemented with 1 mM GMPCPP (Jena Bioscience, NU-405L). A flow chamber was prepared as for assays with taxol-stabilized microtubules ...
-
bioRxiv - Molecular Biology 2019Quote: ... Capturing of ATP binding proteins was performed on 75 μl of this mixture using 30 μl of Aminophenyl-ATP- or naked agarose beads (Jena Bioscience) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... GMPPNP binding Irgb6 crystals diffracting to 1.6 Å resolution were obtained from sitting drops with a 9 mg/ml protein solution containing 2 mM GMPPNP (Jena Bioscience) and a reservoir solution consisting of 0.1 M Sodium citrate buffer pH 5.6 ...
-
bioRxiv - Microbiology 2023Quote: ... Protein-containing fractions were pooled and incubated with an ATP-agarose resin (Immobilized γ-Aminophenyl-ATP C10-spacer from Jena Bioscience) overnight at 4 °C with gentle agitation ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were measured using BCA and 300 µg of protein lysate was added to 50 µl of m7GTP beads (Jena Bioscience) for 2h at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (500 mg of protein) were incubated with 50 μl of 7-methyl-GTP-Agarose slurry (Jena Biosciences, AC-155S) for 16 h at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 mM GTP (Roth, K056.4) or 1 mM GMP (Jena Biosciences, JBS-NU-1028), was added to the washed beads and incubated gently by rocking for 30 min at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 mM GMPCPP (Jena Biosciences) and 4 mM MgCl2 ...
-
bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
-
bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18.000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
-
bioRxiv - Microbiology 2022Quote: ... and 1 μL of 3-azido-7-hydroxycoumarin solution (1 mM in EtOH; Jena Bioscience) was added ...
-
bioRxiv - Neuroscience 2024Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GTP (Jena Bioscience, Jena, Germany) for 30 minutes at 37 °C prior to centrifugation at 18.000 x g for 30 min in a Microfuge 18 Centrifuge (Beckman Coulter ...
-
bioRxiv - Biophysics 2021Quote: ... 1 mM GMPCPP (Jena Bioscience, Germany), 1 mM MgCl2 and 4 mg mL-1 of porcine brain tubulin ...
-
bioRxiv - Cell Biology 2021Quote: ... with 1 mM GMPCPP (Jena Bioscience) in PEM80 (80 mM Pipes ...
-
bioRxiv - Biophysics 2023Quote: ... and Wizard 1&2 (Jena Bioscience) screens were used at 16 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... and 1 mM GTP (Jena Bioscience). A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich) ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were purified from the supernatant by Ni-NTA affinity chromatography using either Ni-NTA agarose beads (Jena Bioscience, for gravity-based purification) or 1 mL Ni-NTA HisTrap column (GE Healthcare ...
-
bioRxiv - Cancer Biology 2021Quote: To fluorescently label IVT RNA the ATTO647N RNA labelling kit (Jena Bioscience) was used ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA was isolated using the Total RNA Purification Kit (Jena Biosciences) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... primer extension was performed using the DNA cycle sequencing kit (Jena Bioscience), 150 ng digested DNA and 32P-end-labelled oligonucleotides according to the manufacturer protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... AF488-Azide (#CLK-1275-1, Jena Bioscience) was used to test success of bead – DBCO coupling ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 mM GMPPCP (NU-405S, Jena Bioscience) or 1 mM BeF4 (prepared by mixing 50 mM BeSO4 and NaF 100 mM to make 10 mM BeF3) ...
-
bioRxiv - Microbiology 2021Quote: ... Labeling was achieved by using a 1:1 molar ratio of dCTP:Biotin-11-dCTP (NU-809-BIOX, Jena Bioscience) in the dNTPs used for the PCR (GoTaq DNA polymerase ...
-
bioRxiv - Microbiology 2023Quote: ... and pellets were resuspended using 1/5 or 1/10 dilutions of detergents stocks from the JBScreen (Jena Bioscience) or Detergent Screen (Hampton) ...
-
bioRxiv - Microbiology 2023Quote: ... A sequencing ladder was generated using the DNA Cycle Sequencing kit (Jena Bioscience) according to the manufacturer’s recommendations with the CJnc230 sRNA region amplified from NCTC11168 WT (CSS-5295 ...
-
bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C in click reagents (1:1 DMSO/H2O with 20 μM picolyl-azide-sulfo-Cy5 (Jena Bioscience), 300 μM CuSO4 ...
-
bioRxiv - Biochemistry 2020Quote: ... JBScreen Classic 1-4 and 6 (Jena Bioscience) and Crystal Screen ...
-
bioRxiv - Cell Biology 2022Quote: ... was incubated with 1 mM GMPCPP (Jena Biosciences) at 37°C for 30 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... and 50 µg mL-1 nourseothricin (Jena Bioscience).
-
bioRxiv - Microbiology 2023Quote: ... JBScreen Classic 1-4 and 6 (Jena Bioscience) and Crystal Screen ...
-
bioRxiv - Cell Biology 2024Quote: ... was incubated with 1 mM GMPCPP (Jena Biosciences) at 37°C for 30 minutes ...
-
bioRxiv - Microbiology 2019Quote: The Click Chemistry labelling system “CuAAC Biomolecule Reaction Buffer Kit (THPTA based)” (Jena Bioscience) was used following manufacturer’s instructions [51] ...