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Citations for Jena Bioscience :
1 - 50 of 53 citations for Rabbit Anti West Nile Virus Envelope Protein Antibody 9E2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Endogenous and overexpressed PML was immunofluorescently labelled with anti-PML antibody (rabbit, ABD-030, Jena Bioscience, Germany, 1:500), followed by secondary antibody coupled with STAR 580 STED dye (goat-anti-rabbit ...
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bioRxiv - Immunology 2023Quote: ... anti-dsRNA monoclonal antibody (Jena Bioscience, Germany ...
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bioRxiv - Microbiology 2021Quote: ... The mouse anti-dsRNA antibody (J2) was from Jena Bioscience and rabbit anti-Calnexin from Elabscience ...
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bioRxiv - Microbiology 2020Quote: ... Genome-labeled AdV was produced by growing the virus in A549 cells in the presence of 2.5 μM EdC (5-ethynyl-2’-deoxycytidine, Jena Biosciences) as described 52 ...
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bioRxiv - Molecular Biology 2023Quote: ... Each fraction was then incubated with anti-dsRNA monoclonal antibody J2 (SCICONS/Jena Bioscience RNT-SCI-10010200 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated with anti-dsRNA monoclonal antibody J2 (SCICONS/Jena Bioscience RNT-SCI-10010200) at a concentration of 2.5 µg/mL overnight at 4 °C followed by anti-mouse secondary antibody.
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bioRxiv - Immunology 2024Quote: ... Immunostaining was performed in four steps to avoid unspecific binding of the secondary antibodies: i) incubation overnight at 4LJ with the mouse monoclonal IgG2a J2 antibody anti-dsRNA (Jena Bioscience, cat. RNT-SCI-10010500, dilution 1:200), ii ...
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bioRxiv - Immunology 2020Quote: ... Vero E6 cells were incubated with a 1:500 dilution of an anti-dsRNA J2 antibody (Jena Bioscience) in PBS supplemented with 1% FCS at 4°C overnight with shaking ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified with 4% Glutathione Agarose (Jena Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were purified by Ni2+-NTA chromatography (Jena Bioscience) and eluted stepwise in binding buffer with 40–250 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... Extracted proteins were incubated overnight with Alkyne agarose beads (Jena Bioscience), and downstream preparations were done using Click Chemistry Capture Kit following the manufacturer’s guidelines ...
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bioRxiv - Biophysics 2019Quote: ... Control reactions for azide-bearing proteins were performed using TAMRA-DBCO (Jena Bioscience). Reaction mixtures were incubated for varying durations (0.5 hours to overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Cap-bound proteins were pulled down using γ-Aminophenyl-m7GTP beads (Jena Bioscience).
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bioRxiv - Microbiology 2022Quote: ... Then GST-tagged proteins were pulled down using Glutathione magnetic agarose (Jena Bioscience). Beads were washed with protein binding buffer and proteins are then eluted using Laemmli buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and 32μg of protein was clicked to 31μM biotin-alkyne (Jena biosciences, #CLK-TA105) mixed in PBS pH 7.8-PI (Calbiochem ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated with ~20× molar excess of Dibenzylcyclooctyne(DBCO)-Sulfo-Cy5 (Jena Bioscience) at 4°C overnight in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were then extracted and probes were clicked onto azide-functionalized magnetic beads (Jena Bioscience) as described above ...
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bioRxiv - Cell Biology 2021Quote: ... Total proteins (500μg) were incubated with 15μL Immobilized γ-Aminophenyl-m7GTP (Jena Bioscience, #AC-155) at 4°C for 3 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative protein occupancy was performed by qPCR according to the manufacturer’s instructions (Jena Bioscience, PCR-369). Mean values for the analyzed regions were expressed as fold enrichment compared to a negative control without protein occupation (SOX2 gene) ...
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bioRxiv - Developmental Biology 2020Quote: ... 250 µg of protein was pre-cleared using 50 µL of agarose beads (Jena Biosciences, AC-100S) in Cap Lysis Buffer for 30 min at 4°C with rocking ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alkyne labelled proteins were enriched and affinity purified by direct attachment to azide agarose beads (Jena Bioscience) using copper catalysed click chemistry ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 μg of protein was incubated overnight at 4°C with m7GTP agarose beads (Jena biosciences, #AC-155S). Beads were washed with cap binding buffer and suspended in 20 μL Laemmli buffer ...
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bioRxiv - Molecular Biology 2022Quote: C15-az and C17-az modified proteins were pulled down using the Click Chemistry Capture Kit (Jena Bioscience) and alkyne agarose beads (Jena Bioscience) ...
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bioRxiv - Microbiology 2023Quote: ... Eluted protein was incubated with an ATP-agarose resin (Immobilized γ-Aminophenyl-ATP C10-spacer from Jena Bioscience) overnight at 4 °C with gentle agitation ...
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bioRxiv - Microbiology 2021Quote: ... crescentus ParBΔCTD-CTPɣS complex crystal were also set up in sitting-drop vapour diffusion format in MRC2 96-well crystallization plates with drops comprised of 0.3 µL precipitant solution and 0.3 µL of protein solution (∼10 mg/mL) supplemented with 1 mM CTPɣS (Jena Biosciences) and 1 mM MgCl2 ...
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bioRxiv - Microbiology 2021Quote: To verify the functionality of mutated SoGGDEF proteins they were tested for GTP binding using MANT-GTP (Jena Bioscience, Germany). Proteins were diluted to 1 μM and 100 μl of each sample was added in triplicates into a white round bottom 96-well plate before MANT-GTP with a final concentration of 1 μM was added ...
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bioRxiv - Neuroscience 2022Quote: ... 250-300 μg of protein lysate together with 30 μl of 7-methyl GTP-agarose beads (Jena bioscience #AC-141) were incubated for 90 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... expression of protein was induced in 45 mL BHI medium supplemented with bleomycin and tetracyclin 10 mg/mL (Jena Bioscience). 5 ml of culture from nonclonal selection was added incubated for 72 h at 26°C with shaking at 100 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 20 μl of the prepared protein were mixed with 5 μl of 500-fold diluted JBS Thermofluor Dye (Jena Bioscience), prepared in the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... or anti-dsRNA J2 (Jena Bioscience, RNT-SCI-10010500), followed by secondary goat anti-mouse antibodies conjugated with Alexa Fluor 488 (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-long dsRNA J2 (Jena Bioscience RNT-SCI-10010200); anti-α-tubulin 12G10 (Developmental Studies Hybridoma Bank of the University of Iowa) ...
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bioRxiv - Immunology 2020Quote: ... purified naïve C57BL/6 B2 cells were preincubated with a mixture of NIP-OVA and HIV-1 p17/p24/gp120 fusion protein (Jena Biosciences) covalently bound to 1 μm beads (1:1 bead:Bcell ratio ...
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bioRxiv - Molecular Biology 2019Quote: ... Capturing of ATP binding proteins was performed on 75 μl of this mixture using 30 μl of Aminophenyl-ATP- or naked agarose beads (Jena Bioscience) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... GMPPNP binding Irgb6 crystals diffracting to 1.6 Å resolution were obtained from sitting drops with a 9 mg/ml protein solution containing 2 mM GMPPNP (Jena Bioscience) and a reservoir solution consisting of 0.1 M Sodium citrate buffer pH 5.6 ...
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bioRxiv - Physiology 2022Quote: ... glycoproteins were enriched using Concanavalin A and azido-modifed proteins were immobilized on magnetic DBCO-beads (Jena Bioscience #CLK-1037-1) via click chemistry ...
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bioRxiv - Microbiology 2023Quote: ... Protein-containing fractions were pooled and incubated with an ATP-agarose resin (Immobilized γ-Aminophenyl-ATP C10-spacer from Jena Bioscience) overnight at 4 °C with gentle agitation ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were measured using BCA and 300 µg of protein lysate was added to 50 µl of m7GTP beads (Jena Bioscience) for 2h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (500 mg of protein) were incubated with 50 μl of 7-methyl-GTP-Agarose slurry (Jena Biosciences, AC-155S) for 16 h at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The chamber was pre-equilibrated with an oxygen scavenger cocktail 32 in 20 mM HEPES pH 7.4 with 150 mM NaCl and time-lapse images were acquired while flowing desired proteins in the same buffer in the absence or presence of 1 mM GTP (Jena Bioscience) and 1 mM MgCl2.
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bioRxiv - Microbiology 2020Quote: ... and expression of the host Leishmania tarentolae strain T7-TR were performed according to the Jena Bioscience protocol for inducible expression of recombinant proteins secreted to medium (LEXSinduce Expression kit, Jena Bioscience, Germany). Expression of proKLK13 and proKLK14 was induced with 15 µg/ml of tetracycline (BioShop ...
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bioRxiv - Immunology 2019Quote: ... suis proteins were amplified and cloned into the vector pLEXSYsat2 of the Leishmania Expression System (LEXSYcon2 Expression Kit, Jena Bioscience GmbH, Jena, Germany). The cloning vector was sequenced for verification ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were purified from the supernatant by Ni-NTA affinity chromatography using either Ni-NTA agarose beads (Jena Bioscience, for gravity-based purification) or 1 mL Ni-NTA HisTrap column (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-dsRNA J2 (1:100, RNT-SCI-10010200; Jena Bioscience), sheep anti-SARS-CoV-2 nsp3 (1:200 ...
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bioRxiv - Biophysics 2020Quote: ... antibodies were reacted with 20-fold excess of a bifunctional DBCO-sulfo-NHS ester (Jena Biosciences, cat: CLK-A124-10). Unreacted linker was removed using Zeba Spin Desalting columns (0.5 ml ...
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bioRxiv - Cell Biology 2024Quote: ... the antibody was incubated with 10-fold excess of bifunctional DBCO-sulfo-NHS ester (Jena Bioscience, Cat.# CLK-A124-10) for 2 hours at +40C ...
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bioRxiv - Biochemistry 2020Quote: ... Additives as indicated were added during the primary antibody incubation step at a final concentration of 1 µM for adenosine (Jena Bioscience), AMP (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... concentrated secondary antibodies were incubated with a 20-fold molar excess of DBCO-sulfo-NHS ester (Jena Bioscience, CLK-A124-10) for 90 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... primary amines of the antibody were reacted with a DBCO-sulfo-NHS ester cross-linker (Jena Bioscience, Cat. No. CLK-A124-10) for two hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... in the presence of [α-32P]UTP and 7 mM “anti-reverse” cap analog (m7,3’-OGpppG; Jena Bioscience). All radiolabeled RNAs were gel-purified ...