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Citations for Jena Bioscience :
1 - 50 of 163 citations for Human Immunodeficiency Virus GP41 Protein HIV 1 Group O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Guanosine nucleotides labeled with fluorescent N-methyl-anthranoyl (mant) groups O-linked with 2’ or 3’ of ribose (Jena Biosciences), were used for binding studies ...
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bioRxiv - Immunology 2020Quote: ... purified naïve C57BL/6 B2 cells were preincubated with a mixture of NIP-OVA and HIV-1 p17/p24/gp120 fusion protein (Jena Biosciences) covalently bound to 1 μm beads (1:1 bead:Bcell ratio ...
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bioRxiv - Biophysics 2019Quote: ... 2’-(3’)-bis-O-(N-methylanthraniloyl)-GDP (Jena Biosciences). Rab5 was loaded with mant-GDP in Rab5 buffer + 10mM EDTA and 5-fold excess mant-GDP ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with O-propargyl-puromycin (OPP, 20μM, Jena Bioscience) for 30’ ...
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bioRxiv - Genomics 2023Quote: 3’-(O-Propargyl)-ATP (NU-945) was offered by Jena Biosciences. To complete the set ...
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bioRxiv - Biophysics 2021Quote: ... we functionalized PNA-cysteine (O - TTACATGACACTACTCCAC) with Azido-PEG3-maleimide (Jena Bioscience) according to the manufacturer’s instructions (Azido-PEG3-Maleimide Preparation Kit) ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 30 μM O-propargyl-puromycin (OPP) (Jena Bioscience, Jena, Germany), 10% FBS and either “heavy” amino acids [0.398 mM L-(13C6,15N4)-arginine (Arg“10” ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μl of 0.22 mM O-propargyl-puromycin (OP-puro, Jena Bioscience) dissolved in Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... Cy3 labeled 2’/3’-O-(2-aminoethyl-carbamoyl)-adenosine-5’-triphosphate (Jena Bioscience) was added into the imaging buffer instead of ATP.
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Tail Length and E525K Dilated Cardiomyopathy Mutant Alter Human β-Cardiac Myosin Super-Relaxed StatebioRxiv - Molecular Biology 2023Quote: The fluorescence of 3’-O-(N-Methyl-anthraniloyl)-2’-deoxyadenosine-5’-triphosphate (mantATP) (Jena Biosciences) was measured with 290 nm excitation and a 395 nm long-pass emission filter in a stopped-flow apparatus (Applied Photophysics ...
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bioRxiv - Biochemistry 2022Quote: Guanine nucleotide exchange activity was measured by following changes in the fluorescence of the GDP derivative mant-dGDP [2’-Deoxy-3’-O-(N-Methyl-anthraniloyl) guanosine-5’-diphosphate sodium salt (Jena Bioscience GmbH)] (Margarit et al. ...
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bioRxiv - Microbiology 2020Quote: ... Genome-labeled AdV was produced by growing the virus in A549 cells in the presence of 2.5 μM EdC (5-ethynyl-2’-deoxycytidine, Jena Biosciences) as described 52 ...
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bioRxiv - Genomics 2022Quote: ... human 22 with Atto-594-dUTPs and human 15 was labelled with Atto-425-dUTPs and Atto-594-dUTPs (Jena Bioscience). Hybridization was carried out overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... crescentus ParBΔCTD-CTPɣS complex crystal were also set up in sitting-drop vapour diffusion format in MRC2 96-well crystallization plates with drops comprised of 0.3 µL precipitant solution and 0.3 µL of protein solution (∼10 mg/mL) supplemented with 1 mM CTPɣS (Jena Biosciences) and 1 mM MgCl2 ...
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bioRxiv - Physiology 2022Quote: ... glycoproteins were enriched using Concanavalin A and azido-modifed proteins were immobilized on magnetic DBCO-beads (Jena Bioscience #CLK-1037-1) via click chemistry ...
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bioRxiv - Biochemistry 2023Quote: ... The chamber was pre-equilibrated with an oxygen scavenger cocktail 32 in 20 mM HEPES pH 7.4 with 150 mM NaCl and time-lapse images were acquired while flowing desired proteins in the same buffer in the absence or presence of 1 mM GTP (Jena Bioscience) and 1 mM MgCl2.
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bioRxiv - Biochemistry 2020Quote: ... transfected cells were treated with 20 ng/ml recombinant human TNFα (Jena Biosciences) for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified with 4% Glutathione Agarose (Jena Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were purified by Ni2+-NTA chromatography (Jena Bioscience) and eluted stepwise in binding buffer with 40–250 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... Extracted proteins were incubated overnight with Alkyne agarose beads (Jena Bioscience), and downstream preparations were done using Click Chemistry Capture Kit following the manufacturer’s guidelines ...
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bioRxiv - Biophysics 2019Quote: ... Control reactions for azide-bearing proteins were performed using TAMRA-DBCO (Jena Bioscience). Reaction mixtures were incubated for varying durations (0.5 hours to overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Cap-bound proteins were pulled down using γ-Aminophenyl-m7GTP beads (Jena Bioscience).
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bioRxiv - Microbiology 2022Quote: ... Then GST-tagged proteins were pulled down using Glutathione magnetic agarose (Jena Bioscience). Beads were washed with protein binding buffer and proteins are then eluted using Laemmli buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and 32μg of protein was clicked to 31μM biotin-alkyne (Jena biosciences, #CLK-TA105) mixed in PBS pH 7.8-PI (Calbiochem ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated with ~20× molar excess of Dibenzylcyclooctyne(DBCO)-Sulfo-Cy5 (Jena Bioscience) at 4°C overnight in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were then extracted and probes were clicked onto azide-functionalized magnetic beads (Jena Bioscience) as described above ...
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bioRxiv - Cell Biology 2021Quote: ... Total proteins (500μg) were incubated with 15μL Immobilized γ-Aminophenyl-m7GTP (Jena Bioscience, #AC-155) at 4°C for 3 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative protein occupancy was performed by qPCR according to the manufacturer’s instructions (Jena Bioscience, PCR-369). Mean values for the analyzed regions were expressed as fold enrichment compared to a negative control without protein occupation (SOX2 gene) ...
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bioRxiv - Developmental Biology 2020Quote: ... 250 µg of protein was pre-cleared using 50 µL of agarose beads (Jena Biosciences, AC-100S) in Cap Lysis Buffer for 30 min at 4°C with rocking ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alkyne labelled proteins were enriched and affinity purified by direct attachment to azide agarose beads (Jena Bioscience) using copper catalysed click chemistry ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 μg of protein was incubated overnight at 4°C with m7GTP agarose beads (Jena biosciences, #AC-155S). Beads were washed with cap binding buffer and suspended in 20 μL Laemmli buffer ...
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bioRxiv - Molecular Biology 2022Quote: C15-az and C17-az modified proteins were pulled down using the Click Chemistry Capture Kit (Jena Bioscience) and alkyne agarose beads (Jena Bioscience) ...
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bioRxiv - Microbiology 2023Quote: ... Eluted protein was incubated with an ATP-agarose resin (Immobilized γ-Aminophenyl-ATP C10-spacer from Jena Bioscience) overnight at 4 °C with gentle agitation ...
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bioRxiv - Biochemistry 2023Quote: ... 0.9 mL of DNase solution (15 mM MgSO4, 1 mM DTT and 1 mM PMSF, 1 x Turbo DNase (Jena biosciences)) was added and the samples were allowed to shake at 37 °C for 10 min before transferring to ice ...
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bioRxiv - Microbiology 2021Quote: To verify the functionality of mutated SoGGDEF proteins they were tested for GTP binding using MANT-GTP (Jena Bioscience, Germany). Proteins were diluted to 1 μM and 100 μl of each sample was added in triplicates into a white round bottom 96-well plate before MANT-GTP with a final concentration of 1 μM was added ...
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bioRxiv - Neuroscience 2022Quote: ... 250-300 μg of protein lysate together with 30 μl of 7-methyl GTP-agarose beads (Jena bioscience #AC-141) were incubated for 90 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... expression of protein was induced in 45 mL BHI medium supplemented with bleomycin and tetracyclin 10 mg/mL (Jena Bioscience). 5 ml of culture from nonclonal selection was added incubated for 72 h at 26°C with shaking at 100 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 20 μl of the prepared protein were mixed with 5 μl of 500-fold diluted JBS Thermofluor Dye (Jena Bioscience), prepared in the same buffer ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with SiR-Tet (1–2 µM, 1 h; Spirochrome, Stein am Rhein, Switzerland) or TAMRA-Tet (2 µM, 1 h; Jena BioScience, Germany) and washed again with fresh medium (3 × quick wash and 3 × 30 min wash ...
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bioRxiv - Cell Biology 2019Quote: ... 12 μM unlabeled tubulin + 1 μM Alexa-568 tubulin + 1 μM biotin tubulin was polymerized in BRB80 (80 mM Pipes, 1 mM EGTA, 1 mM MgCl2) in the presence of 1 mM GMPCPP (Jena Bioscience, NU-405L) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... as in 74 from a mixture of unlabeled, rhodamine- and biotin-labeled tubulins (8:1:1, total tubulin concentration 5 μM) supplemented with 1 mM GMPCPP (Jena Bioscience, NU-405L). A flow chamber was prepared as for assays with taxol-stabilized microtubules ...
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bioRxiv - Molecular Biology 2019Quote: ... Capturing of ATP binding proteins was performed on 75 μl of this mixture using 30 μl of Aminophenyl-ATP- or naked agarose beads (Jena Bioscience) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... GMPPNP binding Irgb6 crystals diffracting to 1.6 Å resolution were obtained from sitting drops with a 9 mg/ml protein solution containing 2 mM GMPPNP (Jena Bioscience) and a reservoir solution consisting of 0.1 M Sodium citrate buffer pH 5.6 ...
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bioRxiv - Microbiology 2023Quote: ... Protein-containing fractions were pooled and incubated with an ATP-agarose resin (Immobilized γ-Aminophenyl-ATP C10-spacer from Jena Bioscience) overnight at 4 °C with gentle agitation ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were measured using BCA and 300 µg of protein lysate was added to 50 µl of m7GTP beads (Jena Bioscience) for 2h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (500 mg of protein) were incubated with 50 μl of 7-methyl-GTP-Agarose slurry (Jena Biosciences, AC-155S) for 16 h at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM GTP (Roth, K056.4) or 1 mM GMP (Jena Biosciences, JBS-NU-1028), was added to the washed beads and incubated gently by rocking for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM GMPCPP (Jena Biosciences) and 4 mM MgCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...