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Citations for Jena Bioscience :
1 - 50 of 134 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: To verify the functionality of mutated SoGGDEF proteins they were tested for GTP binding using MANT-GTP (Jena Bioscience, Germany). Proteins were diluted to 1 μM and 100 μl of each sample was added in triplicates into a white round bottom 96-well plate before MANT-GTP with a final concentration of 1 μM was added ...
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bioRxiv - Immunology 2020Quote: ... purified naïve C57BL/6 B2 cells were preincubated with a mixture of NIP-OVA and HIV-1 p17/p24/gp120 fusion protein (Jena Biosciences) covalently bound to 1 μm beads (1:1 bead:Bcell ratio ...
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bioRxiv - Cancer Biology 2020Quote: ... Binding was measured between EDA-GTP-Cy5 (Jena Bioscience GmbH, Jena, Germany) and His-tagged labeled proteins in Buffer I ...
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bioRxiv - Microbiology 2020Quote: ... ATP binding assays were performed according to the manufacturer’s protocol (Jena Biosciences). Here we used 6AH-ATP-Agarose (N6 - (6-Amino ...
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bioRxiv - Biochemistry 2021Quote: ... ATP-FAM [g-(6-Aminohexyl)-ATP-6-FAM] was purchased from Jena Bioscience. Reactions were incubated for 30 minutes at room temperature before taking fluorescence polarization measurements on a Victor3Vplate reader (PerkinElmer) ...
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bioRxiv - Biochemistry 2020Quote: ... JBScreen Classic 1-4 and 6 (Jena Bioscience) and Crystal Screen ...
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bioRxiv - Molecular Biology 2022Quote: ... and supplements with 6 mM THTPA (Jena Bioscience).
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bioRxiv - Microbiology 2023Quote: ... JBScreen Classic 1-4 and 6 (Jena Bioscience) and Crystal Screen ...
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bioRxiv - Cell Biology 2024Quote: ... employing 2 mM 6-FAM-dC-Puromycin (Jena Bioscience), according to Wang et al ...
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bioRxiv - Molecular Biology 2022Quote: C15-az and C17-az modified proteins were pulled down using the Click Chemistry Capture Kit (Jena Bioscience) and alkyne agarose beads (Jena Bioscience) ...
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bioRxiv - Cell Biology 2020Quote: ... The incorporated EdU was visualized by click-it chemistry-mediated binding of Biotin (Azide-PEG3-Biotin Conjugate; Jena Biosciences) using the protocol and reagents from the Invitrogen Click-iT EdU Cell Proliferation Kit for Imaging (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: Nucleotide release and binding measurements were performed using the fluorescent nucleotide analogues MABA-ADP (Jena Bioscience, NU-893-MNT) and MABA-ATP (Jena Bioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... 60 μM UDP-6-N3-Glc (Jena Bioscience, CLK-076Motif) and 12.5 U T4 beta-glucosyltransferase (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 60 μM UDP-6-N3-Glc (Jena Bioscience, CLK-076) and 12.5 U T4 beta-glucosyltransferase (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 0,2 µM 5/6 TAMRA-PEG4-Alkyne tag (Jena Biosciences) and 0,2 mM freshly prepared CuSO4 ...
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bioRxiv - Cell Biology 2022Quote: Nucleotide binding assays were carried out by measuring the incorporation of 2,3-0-N-Methyl-anthraniloyl (Mant)-labelled nucleotides (Jena Bioscience) using an Synergy2 plate reader (BioTek Instruments Inc. ...
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bioRxiv - Genomics 2022Quote: ... human 22 with Atto-594-dUTPs and human 15 was labelled with Atto-425-dUTPs and Atto-594-dUTPs (Jena Bioscience). Hybridization was carried out overnight at 37°C ...
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bioRxiv - Biochemistry 2023Quote: Nucleotide binding was analyzed using the non-hydrolysable GTP analogue N- methylanthraniloyl guanosine-5’-[(β,γ)-imido]triphosphate (mant-GMPPNP, Jena Bioscience). Varying protein concentrations of non-farnesylated GBP1 ranging from 0.02 µM to 40 µM were prepared in a 96-well plate in 50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Molecular Biology 2022Quote: ... fluorescent puromycin (6-FAM-dC-Puromycin, Jena Bioscience #NU-925-6FM-L) was added to 4.6 µM ...
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bioRxiv - Microbiology 2020Quote: ... and expression of the host Leishmania tarentolae strain T7-TR were performed according to the Jena Bioscience protocol for inducible expression of recombinant proteins secreted to medium (LEXSinduce Expression kit, Jena Bioscience, Germany). Expression of proKLK13 and proKLK14 was induced with 15 µg/ml of tetracycline (BioShop ...
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bioRxiv - Biophysics 2019Quote: ... and 6-methyl-tetrazine-PEG5-NHS-ester (Jena Bioscience, Figure S12, Supplementary Information) were conjugated to 3’-amine modified DNA oligos (Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... For labeling pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl)phosphate (Jena bioscience), was ligated to the 3’ end of the 16 nt RNA using T4 RNA ligase (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... and 1× TranscriptAid enzyme mix and 6 mM cap analog m7GpppG (Jena Bioscience) or m7GpppA (prepared chemically according to a published protocol40) ...
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bioRxiv - Immunology 2023Quote: ... we site-specifically decorated PS-CFP2 with 6-Methyl-Tetrazine-PEG4Maleimide (Jena Bioscience) and mSav (A106C ...
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bioRxiv - Immunology 2019Quote: ... suis proteins were amplified and cloned into the vector pLEXSYsat2 of the Leishmania Expression System (LEXSYcon2 Expression Kit, Jena Bioscience GmbH, Jena, Germany). The cloning vector was sequenced for verification ...
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bioRxiv - Biophysics 2019Quote: ... we site-specifically conjugated PS-CFP2* with 6-Methyl-Tetrazine-PEG4-Maleimide (Jena Bioscience) and the monovalent streptavidin (mSav* ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 μM 5/6- TAMRA-PEG 4 -Alkyne (Jena Bioscience; Jena, Germany; CLK-TA108) and 0.2 mM freshly prepared CuSO4 in 1x PBS pH7.8 ...
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bioRxiv - Molecular Biology 2024Quote: Cells seeded in 6-well plates were treated with 20 μM OP-puro (Jena Bioscience) at 37°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... transfected cells were treated with 20 ng/ml recombinant human TNFα (Jena Biosciences) for 30 min ...
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bioRxiv - Genomics 2021Quote: ... 1 μL of 0.05 mM 7-propargylamino-7-deaza-ddATP-6-FAM (Jena Bioscience, Jena, Germany), 0.4 μL of internal standard solution (see Supplementary Methods) ...
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bioRxiv - Molecular Biology 2022Quote: ... 24% PEG 3350 and 1mM Anderson−Evans polyoxotungstate [TeW6O24]6− (TEW) (Jena Biosciences #X-TEW-5) to improve crystal diffraction ...
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bioRxiv - Immunology 2024Quote: ... Immunostaining was performed in four steps to avoid unspecific binding of the secondary antibodies: i) incubation overnight at 4LJ with the mouse monoclonal IgG2a J2 antibody anti-dsRNA (Jena Bioscience, cat. RNT-SCI-10010500, dilution 1:200), ii ...
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bioRxiv - Physiology 2023Quote: ... the supernatant was incubated with 150 μl 6% (v/v) Nickel-IDA agarose bead suspension (Jena Bioscience) for 2 hours at 4 °C under mild agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified with 4% Glutathione Agarose (Jena Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were purified by Ni2+-NTA chromatography (Jena Bioscience) and eluted stepwise in binding buffer with 40–250 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... Cytarabine-TP (NU- 1170S) and N6-(6-Aminohexyl)-ATP-6FAM (NU-805-6FM) were purchased from Jena Bioscience.
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bioRxiv - Biophysics 2019Quote: ... Monomeric PS-CFP2*-AVI-3C-12xHis was site-specifically conjugated with 6-Methyl-Tetrazine-PEG4-Maleimide (Jena Bioscience) to arrive at PS-CFP2*-tetrazine and further processed as described below.
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bioRxiv - Biochemistry 2020Quote: ... incubated with 50 μg/ml of lysozyme and 6 U of RNase-free DNase I (Jena Bioscience, Germany) at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were labeled at the 3’ end using pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl) phosphate) (Jena Biosciences). For each binding experiment ...
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bioRxiv - Molecular Biology 2021Quote: ... Extracted proteins were incubated overnight with Alkyne agarose beads (Jena Bioscience), and downstream preparations were done using Click Chemistry Capture Kit following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2022Quote: ... short oligo DNAs for each target (see Supplementary Table 6) were pooled and labelled with 5-Propargylamino-ddUTP-Cy3 or -Cy5 (Jena Biosciences) using the Terminal Deoxynucleotidyl Transferase (Thermo Fisher)52 ...
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bioRxiv - Cell Biology 2023Quote: On-gel mito-FUNCAT was performed as described previously.72,114 HeLa S3 and MPV17L2 KO #1 cells were cultured in 6-well plates in methionine-free medium with 50 µM HPG (Jena Bioscience) and 100 µg/ml anisomycin (Alomone Labs ...
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bioRxiv - Biophysics 2019Quote: ... Control reactions for azide-bearing proteins were performed using TAMRA-DBCO (Jena Bioscience). Reaction mixtures were incubated for varying durations (0.5 hours to overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Cap-bound proteins were pulled down using γ-Aminophenyl-m7GTP beads (Jena Bioscience).
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bioRxiv - Microbiology 2022Quote: ... Then GST-tagged proteins were pulled down using Glutathione magnetic agarose (Jena Bioscience). Beads were washed with protein binding buffer and proteins are then eluted using Laemmli buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and 32μg of protein was clicked to 31μM biotin-alkyne (Jena biosciences, #CLK-TA105) mixed in PBS pH 7.8-PI (Calbiochem ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... All exchange experiments reported in this article were initiated by incubation of G-actin (1 µM) with N6-(6-Amino)hexyl-ATP-ATTO-488 (0.1 µM; Jena Bioscience, Germany, ref. NU-805-488) in G+ME buffer (5mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated with ~20× molar excess of Dibenzylcyclooctyne(DBCO)-Sulfo-Cy5 (Jena Bioscience) at 4°C overnight in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were then extracted and probes were clicked onto azide-functionalized magnetic beads (Jena Bioscience) as described above ...